FastQCFastQC Report
Sat 18 Jun 2016
SRR3552883_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552883_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences551556
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTGCT58541.061360949749436Illumina Single End Adapter 2 (95% over 21bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCC44180.8010066067634112No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGT39080.7085409278477616No Hit
AATCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCT36830.6677472459732103No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32510.5894233767740719No Hit
AAACTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCT11810.21412150352820022No Hit
AATGACTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTT9420.170789548114788No Hit
AATGATCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCT7910.1434124549456447No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7850.14232462342899No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG7560.13706677109849227No Hit
CCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTGC7490.13579763432906178No Hit
AAAAACTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTT7130.12927064522913356No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCAGTACGTTCGTAT6890.12491931916251478No Hit
AACTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTG6820.12365018239308428No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6250.11331578298486462No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5580.10116833104888716No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTG207.03009E-445.0000041
TCGTTAC207.03009E-445.00000414
GCTCTAG302.1634405E-645.0000041
CAAGGCA207.03009E-445.00000419
TCGCTAG207.03009E-445.00000415
TACCTAG207.03009E-445.0000041
ACCGTAA207.03009E-445.00000410
CGCCGAA207.03009E-445.00000443
GATACTA253.8881582E-545.09
TTCAACG351.2103919E-745.01
TCTAGCG253.8881582E-545.01
CACTACG351.2103919E-745.01
CGCTAAG351.2103919E-745.01
CATACCG253.8881582E-545.01
CGTAACG453.8380676E-1045.01
GCAATAG351.2103919E-745.01
TGTACGA253.8881582E-545.016
CTCGATC1550.043.5483916
TCGATCA1600.042.18750417
ACGGGCT3050.042.0491835