##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552883_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	551556
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.06479487123701	31.0	31.0	33.0	28.0	34.0
2	31.35372836121808	31.0	31.0	34.0	30.0	34.0
3	31.54990064472148	31.0	31.0	34.0	30.0	34.0
4	35.49659508735287	37.0	35.0	37.0	33.0	37.0
5	34.84333957023403	35.0	35.0	37.0	32.0	37.0
6	34.963688183974064	35.0	35.0	37.0	32.0	37.0
7	34.959605189681554	36.0	35.0	37.0	32.0	37.0
8	35.37758450637832	37.0	35.0	37.0	33.0	37.0
9	36.857450558057565	39.0	37.0	39.0	33.0	39.0
10	36.23826229793529	38.0	35.0	39.0	32.0	39.0
11	36.46286505812646	38.0	35.0	39.0	32.0	39.0
12	36.174471857798665	37.0	35.0	39.0	32.0	39.0
13	35.871425566941525	37.0	35.0	39.0	31.0	39.0
14	36.88436894893719	39.0	35.0	40.0	31.0	41.0
15	36.85600192908789	38.0	35.0	40.0	32.0	41.0
16	36.7549315028755	38.0	35.0	40.0	31.0	41.0
17	36.678148728324956	38.0	35.0	40.0	31.0	41.0
18	36.618849219299584	38.0	35.0	40.0	31.0	41.0
19	36.719237937761534	38.0	35.0	40.0	31.0	41.0
20	36.57752612608692	38.0	35.0	40.0	31.0	41.0
21	36.734766732661775	38.0	35.0	40.0	31.0	41.0
22	36.699693231512306	39.0	35.0	40.0	31.0	41.0
23	36.65152949111241	38.0	35.0	40.0	31.0	41.0
24	36.158857849429616	38.0	34.0	40.0	30.0	41.0
25	36.433997635779505	38.0	35.0	40.0	31.0	41.0
26	36.24167264974001	38.0	35.0	40.0	30.0	41.0
27	36.20016462516952	38.0	35.0	40.0	30.0	41.0
28	36.23125122381045	38.0	35.0	40.0	30.0	41.0
29	35.630392924743816	38.0	34.0	40.0	28.0	41.0
30	35.87594006773564	38.0	34.0	40.0	30.0	41.0
31	35.71911102408459	38.0	34.0	40.0	29.0	41.0
32	35.6658652974494	38.0	34.0	40.0	29.0	41.0
33	35.73293736266127	38.0	34.0	40.0	29.0	41.0
34	35.58091834736636	38.0	34.0	40.0	28.0	41.0
35	35.584763831777735	38.0	34.0	40.0	29.0	41.0
36	35.524033824307956	38.0	34.0	40.0	28.0	41.0
37	35.43907055675217	38.0	34.0	40.0	28.0	41.0
38	35.35835345821639	38.0	34.0	40.0	28.0	41.0
39	35.319374641922124	38.0	34.0	40.0	28.0	41.0
40	35.20748210517155	38.0	34.0	40.0	27.0	41.0
41	35.323764042091824	38.0	34.0	40.0	28.0	41.0
42	35.26446815917151	38.0	34.0	40.0	28.0	41.0
43	35.25840349846616	38.0	34.0	40.0	28.0	41.0
44	35.202354792623055	37.0	34.0	40.0	28.0	41.0
45	34.99037450413013	37.0	34.0	40.0	27.0	41.0
46	34.9980273988498	37.0	34.0	40.0	27.0	41.0
47	34.88934940423094	37.0	34.0	40.0	27.0	41.0
48	34.93480988331194	37.0	34.0	40.0	27.0	41.0
49	34.81394273654897	37.0	34.0	40.0	27.0	41.0
50	34.743275388174546	37.0	34.0	40.0	27.0	41.0
51	33.518101516437135	35.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	2.0
10	10.0
11	12.0
12	21.0
13	24.0
14	38.0
15	60.0
16	136.0
17	215.0
18	466.0
19	761.0
20	1306.0
21	1850.0
22	2635.0
23	3119.0
24	3570.0
25	4325.0
26	5033.0
27	6147.0
28	7612.0
29	9918.0
30	13144.0
31	17551.0
32	23938.0
33	33620.0
34	51916.0
35	56118.0
36	64123.0
37	86160.0
38	99496.0
39	58225.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.70552400844157	34.851583520077746	20.172022423833663	25.27087004764702
2	23.25348650001088	34.331781360369575	19.271479233296347	23.1432529063232
3	20.305100479371088	30.51657492620876	22.780642400771637	26.397682193648514
4	22.567246118254538	31.239620274278586	19.380262384961817	26.81287122250506
5	17.129538977003243	36.633451544358145	18.462132584905248	27.774876893733364
6	20.196136022452844	36.08790403875581	22.26174676732734	21.454213171464005
7	76.27022460094713	9.671184793565839	8.045783202430941	6.012807403056081
8	78.38823256387384	6.265184314919972	6.413854622196115	8.932728499010073
9	73.44041221562271	9.437301017485078	11.026260252812046	6.096026514080166
10	36.90831030756623	31.80674310496124	14.25820768879316	17.026738898679373
11	29.206644474903726	25.61408089115158	25.466498415392092	19.712776218552605
12	29.71031046711485	23.563155871751917	28.80378420323594	17.922749457897293
13	24.602760191168258	29.374351833721324	24.55543952019378	21.467448454916635
14	18.220633988207908	30.483758675456347	30.191675913234555	21.10393142310119
15	16.532682084865364	30.112082907265993	29.610955188593724	23.744279819274926
16	19.39531072094221	27.64923235355975	30.349048872643934	22.606408052854107
17	18.131069193336668	28.432471045551132	28.36556940727687	25.070890353835328
18	20.370189065117593	27.915207159381822	26.76663838304723	24.94796539245335
19	21.50606647375788	31.78698083240868	27.00831828499735	19.698634408836092
20	23.607938269187535	30.927956544757013	25.480277614603054	19.9838275714524
21	21.695711768161345	29.601889925954932	25.83726765731857	22.86513064856515
22	19.628469276011863	28.914017796923613	27.1869039589815	24.270608968083025
23	22.064305347054514	28.362305912726903	23.979251426872338	25.594137313346245
24	18.82891311127066	30.73994299762853	27.408821588379055	23.022322302721754
25	20.167489792514267	28.005678480516938	25.728847116158647	26.097984610810144
26	22.14788706858415	31.406421106832305	23.394723291923214	23.050968532660328
27	19.115919326414723	28.6737883369957	26.613254139198922	25.597038197390653
28	20.318879678582046	27.849030742118664	27.638172733140426	24.193916846158867
29	19.008405311518686	32.425175322179435	26.386985183734744	22.179434182567135
30	23.32872817991283	26.934708352370386	24.813255589640942	24.923307878075843
31	24.32808273321295	29.18923917063725	23.65924765572308	22.82343044042672
32	23.962208733111414	28.561379080274712	23.70276091638927	23.7736512702246
33	23.434791752786662	25.50819862353052	25.277034426241396	25.779975197441416
34	20.653931785711695	28.69046842025107	25.231708113047453	25.42389168098978
35	21.061687299204433	26.2435727287891	27.50183118305303	25.19290878895343
36	23.62588748921234	28.18226979672055	27.53646048633321	20.655382227733902
37	22.40715358005352	30.375700744801982	24.65515740922046	22.56198826592404
38	21.840574665129196	27.403563736048557	28.238837035586595	22.517024563235648
39	21.715111430208356	27.358600033360165	26.506102734808433	24.420185801623045
40	23.358280936115282	25.537751379732974	25.1736904321592	25.930277251992546
41	21.139467252645243	22.78807591613544	30.813371625002723	25.259085206216596
42	24.113054703420865	25.19580967299785	25.9605552292061	24.730580394375185
43	20.92770271740313	24.458622515211513	28.510250998991943	26.10342376839342
44	22.340251941779258	23.70620571619201	26.625945506893228	27.32759683513551
45	24.622703768973594	26.18084111132868	26.618149381023866	22.57830573867386
46	20.083726765731857	25.582896387674143	30.95660277469559	23.376774071898414
47	21.179354408255914	26.091820232215767	26.568834352268855	26.159991007259464
48	21.51204954709948	22.70467549985858	33.3889940459355	22.394280907106438
49	22.648470871498088	22.817628672337893	29.707953498828772	24.825946957335248
50	20.33465323557354	22.897040373053688	27.715046160317357	29.053260231055418
51	19.63626540188122	23.281407508938347	31.829950177316533	25.252376911863887
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3782.0
1	3004.0
2	2226.0
3	1557.0
4	888.0
5	1126.5
6	1365.0
7	1282.5
8	1200.0
9	1081.5
10	963.0
11	1018.5
12	1074.0
13	1052.0
14	1030.0
15	1067.0
16	1104.0
17	1114.0
18	1124.0
19	1381.5
20	1639.0
21	1562.0
22	1485.0
23	2053.5
24	2622.0
25	3073.0
26	3863.5
27	4203.0
28	5224.0
29	6245.0
30	7428.0
31	8611.0
32	10011.5
33	11412.0
34	12179.0
35	12946.0
36	13763.0
37	14580.0
38	16001.0
39	17422.0
40	20012.5
41	22603.0
42	26004.5
43	29406.0
44	38704.5
45	48003.0
46	51543.0
47	55083.0
48	57392.5
49	59702.0
50	54834.0
51	49966.0
52	45507.0
53	41048.0
54	36543.5
55	32039.0
56	28136.0
57	24233.0
58	23010.0
59	21787.0
60	19683.5
61	17580.0
62	16647.0
63	15714.0
64	13155.0
65	10596.0
66	8673.5
67	6751.0
68	6234.5
69	5718.0
70	4832.5
71	3947.0
72	3518.5
73	3090.0
74	2412.5
75	1424.5
76	1114.0
77	921.0
78	728.0
79	805.5
80	883.0
81	563.0
82	243.0
83	158.5
84	74.0
85	58.5
86	43.0
87	28.5
88	14.0
89	9.0
90	4.0
91	3.0
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	1.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	551556.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.48358753107197
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.83818957410428	25.284636442905896
2	9.355708792704547	6.078139709660757
3	3.245399664829828	3.1626667225743406
4	1.4908081394535124	1.9370718675989087
5	0.90145780333474	1.4641291730095947
6	0.6274681893521571	1.222945071107042
7	0.463448447866904	1.0538127755696813
8	0.37803222390085744	0.982387426771944
9	0.3268755292486124	0.95562808795015
>10	4.74490403533442	37.599454743159065
>50	0.5642062938850761	11.312815936687842
>100	0.05450997062435494	3.545739866622957
>500	0.005619584600448963	1.3307477155931506
>1k	0.0028097923002244813	3.0012103144124813
>5k	5.619584600448963E-4	1.0686141463761734
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTGCT	5854	1.061360949749436	Illumina Single End Adapter 2 (95% over 21bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCC	4418	0.8010066067634112	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGT	3908	0.7085409278477616	No Hit
AATCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCT	3683	0.6677472459732103	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3251	0.5894233767740719	No Hit
AAACTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCT	1181	0.21412150352820022	No Hit
AATGACTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTT	942	0.170789548114788	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCT	791	0.1434124549456447	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	785	0.14232462342899	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	756	0.13706677109849227	No Hit
CCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTGC	749	0.13579763432906178	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTT	713	0.12927064522913356	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCAGTACGTTCGTAT	689	0.12491931916251478	No Hit
AACTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTG	682	0.12365018239308428	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	625	0.11331578298486462	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	558	0.10116833104888716	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.439157583273503E-4	0.0	0.0	1.3046725989745376	0.0
2	5.439157583273503E-4	0.0	0.0	1.6640196099761402	0.0
3	5.439157583273503E-4	0.0	0.0	2.205034484259078	0.0
4	5.439157583273503E-4	0.0	0.0	4.84030633335509	0.0
5	5.439157583273503E-4	0.0	0.0	5.154870946921074	0.0
6	5.439157583273503E-4	0.0	0.0	6.78770605341978	0.0
7	5.439157583273503E-4	0.0	0.0	7.988128132048241	0.0
8	5.439157583273503E-4	0.0	0.0	8.643727926085475	0.0
9	5.439157583273503E-4	0.0	0.0	10.525676449898107	0.0
10	5.439157583273503E-4	0.0	0.0	12.015280406703942	0.0
11	5.439157583273503E-4	0.0	0.0	14.59271588016448	0.0
12	5.439157583273503E-4	0.0	0.0	15.46987069309372	0.0
13	5.439157583273503E-4	0.0	0.0	15.841909071789628	0.0
14	5.439157583273503E-4	0.0	0.0	16.37422129393933	0.0
15	5.439157583273503E-4	0.0	0.0	16.719607800477196	0.0
16	5.439157583273503E-4	0.0	0.0	17.409292982036277	0.0
17	5.439157583273503E-4	0.0	0.0	18.186729905938837	0.0
18	5.439157583273503E-4	0.0	0.0	19.24428344537998	0.0
19	5.439157583273503E-4	0.0	0.0	19.75157554264662	0.0
20	5.439157583273503E-4	0.0	0.0	20.239105367360704	0.0
21	5.439157583273503E-4	0.0	0.0	20.791542472568516	0.0
22	5.439157583273503E-4	0.0	0.0	21.33600214665419	0.0
23	5.439157583273503E-4	0.0	0.0	21.897323209248018	0.0
24	5.439157583273503E-4	0.0	0.0	22.311424406587907	0.0
25	5.439157583273503E-4	0.0	0.0	22.676029269920008	0.0
26	5.439157583273503E-4	0.0	0.0	22.986786473177702	0.0
27	5.439157583273503E-4	0.0	0.0	23.34069432659603	0.0
28	5.439157583273503E-4	0.0	0.0	23.672845549681266	0.0
29	5.439157583273503E-4	0.0	0.0	24.0394447707939	0.0
30	5.439157583273503E-4	0.0	0.0	24.475302598466882	0.0
31	5.439157583273503E-4	0.0	0.0	24.81561255792703	0.0
32	5.439157583273503E-4	0.0	0.0	25.160999064464896	0.0
33	5.439157583273503E-4	0.0	0.0	25.498045529375077	0.0
34	5.439157583273503E-4	0.0	0.0	25.82512020538259	0.0
35	5.439157583273503E-4	0.0	0.0	26.195164226297965	0.0
36	5.439157583273503E-4	0.0	0.0	26.520969765536048	0.0
37	5.439157583273503E-4	0.0	0.0	26.866718882579466	0.0
38	5.439157583273503E-4	0.0	0.0	27.21917629397559	0.0
39	7.252210111031337E-4	0.0	0.0	27.57000195809673	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTG	20	7.03009E-4	45.000004	1
TCGTTAC	20	7.03009E-4	45.000004	14
GCTCTAG	30	2.1634405E-6	45.000004	1
CAAGGCA	20	7.03009E-4	45.000004	19
TCGCTAG	20	7.03009E-4	45.000004	15
TACCTAG	20	7.03009E-4	45.000004	1
ACCGTAA	20	7.03009E-4	45.000004	10
CGCCGAA	20	7.03009E-4	45.000004	43
GATACTA	25	3.8881582E-5	45.0	9
TTCAACG	35	1.2103919E-7	45.0	1
TCTAGCG	25	3.8881582E-5	45.0	1
CACTACG	35	1.2103919E-7	45.0	1
CGCTAAG	35	1.2103919E-7	45.0	1
CATACCG	25	3.8881582E-5	45.0	1
CGTAACG	45	3.8380676E-10	45.0	1
GCAATAG	35	1.2103919E-7	45.0	1
TGTACGA	25	3.8881582E-5	45.0	16
CTCGATC	155	0.0	43.54839	16
TCGATCA	160	0.0	42.187504	17
ACGGGCT	305	0.0	42.049183	5
>>END_MODULE