FastQCFastQC Report
Sat 18 Jun 2016
SRR3552880_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552880_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences644375
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGCT70331.0914451988360816Illumina Single End Adapter 1 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38880.6033753637245393No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCC20280.3147235693501455No Hit
AATCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCT18710.2903588748787585No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGT18040.27996120271580993No Hit
AAACTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCT16950.26304558680892337No Hit
AAAAACTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTT10720.1663627546071775No Hit
CCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGC10500.1629485935984481Illumina Single End Adapter 1 (95% over 21bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8830.13703200775945684No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG8390.13020368574199806No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8320.1291173617846751No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7040.10925315227934043No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACAG302.1640099E-645.0000041
TCCCGCG302.1640099E-645.00000415
GTTCACG302.1640099E-645.0000041
CGAGACG302.1640099E-645.0000041
ATGCACG302.1640099E-645.0000041
GTACCTA207.030978E-445.021
TACCCCG253.8888917E-545.01
CTATCCG207.030978E-445.01
ATTCGCT207.030978E-445.023
CGATTGA207.030978E-445.015
TAACGCG207.030978E-445.01
TAGCCGT207.030978E-445.044
AGTATTC253.8888917E-545.019
ATCGTAG207.030978E-445.012
GTAATCG207.030978E-445.035
AATGCGC207.030978E-445.034
ACGATAG406.8066583E-945.01
ATTCACG351.2108103E-745.01
CTAAACG207.030978E-445.01
ACGTGTA253.8888917E-545.028