##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552880_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	644375
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.103042483026186	31.0	31.0	33.0	28.0	34.0
2	31.39591231813773	31.0	31.0	34.0	30.0	34.0
3	31.60931600387973	31.0	31.0	34.0	30.0	34.0
4	35.54560620756547	37.0	35.0	37.0	33.0	37.0
5	34.8854657613967	35.0	35.0	37.0	32.0	37.0
6	34.980421338506304	35.0	35.0	37.0	32.0	37.0
7	35.04649311348206	36.0	35.0	37.0	32.0	37.0
8	35.38414277400582	37.0	35.0	37.0	33.0	37.0
9	36.941400581959265	39.0	37.0	39.0	33.0	39.0
10	36.29389873908826	38.0	35.0	39.0	32.0	39.0
11	36.453326091173615	38.0	35.0	39.0	32.0	39.0
12	36.08748011639185	37.0	35.0	39.0	32.0	39.0
13	35.83944597478177	37.0	35.0	39.0	31.0	39.0
14	36.80967914645975	39.0	35.0	40.0	31.0	41.0
15	36.7471518913676	38.0	35.0	40.0	31.0	41.0
16	36.685670611057226	38.0	35.0	40.0	31.0	41.0
17	36.634618040737145	38.0	35.0	40.0	31.0	41.0
18	36.51248419010669	38.0	35.0	40.0	31.0	41.0
19	36.673270999030066	38.0	35.0	40.0	31.0	41.0
20	36.52406750727449	38.0	35.0	40.0	31.0	41.0
21	36.632358486905915	38.0	35.0	40.0	31.0	41.0
22	36.65322832201746	38.0	35.0	40.0	31.0	41.0
23	36.55700019398642	38.0	35.0	40.0	31.0	41.0
24	36.12041125121242	38.0	34.0	40.0	30.0	41.0
25	36.35802444228904	38.0	35.0	40.0	30.0	41.0
26	36.21287138700291	38.0	35.0	40.0	30.0	41.0
27	36.08912046556741	38.0	35.0	40.0	30.0	41.0
28	36.138129194956356	38.0	35.0	40.0	30.0	41.0
29	35.813611639185254	38.0	34.0	40.0	29.0	41.0
30	35.84950999030068	38.0	34.0	40.0	30.0	41.0
31	35.82347546071775	38.0	34.0	40.0	30.0	41.0
32	35.76394490785645	38.0	34.0	40.0	29.0	41.0
33	35.62911658583899	38.0	34.0	40.0	29.0	41.0
34	35.64897303588749	38.0	34.0	40.0	29.0	41.0
35	35.47056450048497	38.0	34.0	40.0	28.0	41.0
36	35.48978467507275	38.0	34.0	40.0	28.0	41.0
37	35.338939282250244	38.0	34.0	40.0	27.0	41.0
38	35.2979879728419	38.0	34.0	40.0	27.0	41.0
39	35.27229641125121	38.0	34.0	40.0	27.0	41.0
40	35.00255906886518	37.0	34.0	40.0	26.0	41.0
41	35.147681086323956	37.0	34.0	40.0	27.0	41.0
42	35.10379204655674	37.0	34.0	40.0	27.0	41.0
43	35.13976333656644	37.0	34.0	40.0	27.0	41.0
44	35.06001319107663	37.0	34.0	40.0	27.0	41.0
45	34.887466149369544	37.0	34.0	40.0	26.0	41.0
46	34.81364578079535	37.0	33.0	40.0	26.0	41.0
47	34.68469602327837	37.0	33.0	40.0	26.0	41.0
48	34.73030611057226	37.0	33.0	40.0	26.0	41.0
49	34.618113676042675	37.0	33.0	40.0	26.0	41.0
50	34.535224054316195	37.0	33.0	40.0	26.0	41.0
51	33.26368962172648	35.0	31.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	9.0
11	6.0
12	21.0
13	23.0
14	41.0
15	75.0
16	142.0
17	256.0
18	500.0
19	949.0
20	1502.0
21	2306.0
22	3042.0
23	3674.0
24	4448.0
25	5097.0
26	6037.0
27	7398.0
28	9291.0
29	11840.0
30	15498.0
31	20833.0
32	28415.0
33	40515.0
34	62420.0
35	66165.0
36	74218.0
37	98712.0
38	115169.0
39	65764.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.62199806013579	33.692647914645974	20.266925315227937	25.418428709990298
2	24.321551891367605	31.815945683802134	20.05385063045587	23.808651794374395
3	21.336023278370515	32.33520853540252	20.543782735208534	25.78498545101843
4	20.803103782735207	32.245819592628514	19.947856450048494	27.003220174587778
5	17.700407371483994	34.26420950533463	19.4039185257032	28.63146459747818
6	20.765548011639183	36.35584869059166	21.595033947623666	21.28356935014549
7	77.59860329776916	7.942424830261882	8.099165858389913	6.359806013579049
8	79.63049466537342	6.34537342386033	6.735984481086324	7.288147429679922
9	73.90168768186227	9.12403491755577	11.540795344325899	5.433482056256063
10	37.85000969932105	30.187390882638216	14.84709990300679	17.115499515033946
11	31.233055286129968	26.410708050436472	24.721629485935985	17.634607177497575
12	30.5094083414161	23.604888457807956	27.862812803103782	18.02289039767216
13	26.14797284190107	29.052337536372452	24.67693501454898	20.122754607177498
14	18.549291949563532	30.76034917555771	29.969194956353057	20.721163918525704
15	17.48360814742968	30.57800193986421	30.188942774005817	21.74944713870029
16	20.277322987390882	27.130940834141608	30.70230843840931	21.889427740058196
17	19.03286129970902	27.99612027158099	28.27592628516004	24.695092143549953
18	21.531871968962175	26.88605237633366	27.344636275460715	24.237439379243455
19	23.035033947623667	31.922250242483024	26.787507274490785	18.255208535402524
20	24.682987390882637	29.735945683802136	25.689388942774006	19.89167798254122
21	22.572570320077595	30.804578079534433	25.203026188166827	21.419825412221144
22	20.141377303588747	28.259165858389913	27.26983511154219	24.329621726479147
23	22.06960232783705	28.594839961202716	24.209350145489815	25.126207565470416
24	19.254005819592628	29.601241513094084	27.65470417070805	23.490048496605237
25	20.320620756547044	27.282250242483023	26.292764306498544	26.10436469447139
26	20.758719689621728	29.806401551891366	25.021765276430653	24.413113482056257
27	16.998486905916586	29.65074684772066	27.98680892337536	25.36395732298739
28	18.859980601357908	26.894898157129	28.430805043646945	25.814316197866148
29	19.34851600387973	28.91235693501455	27.209621726479146	24.529505334626574
30	20.746614936954412	27.67037827352085	28.254510184287103	23.328496605237632
31	24.182657613967024	27.273714839961205	22.380601357904947	26.16302618816683
32	23.263317167798252	26.502735208535405	26.798991270611054	23.434956353055288
33	22.285160038797283	27.79390882638215	25.01493695441319	24.90599418040737
34	20.260407371483996	25.584481086323958	26.743899127061106	27.411212415130944
35	18.671270611057224	28.50514064015519	27.93745877788555	24.886129970902036
36	22.40605237633366	27.704830261881668	27.872124151309407	22.016993210475267
37	19.968341416100873	30.709291949563532	25.847526673132883	23.474839961202715
38	21.09066925315228	29.356818622696412	27.136682832201746	22.415829291949564
39	20.932531522793404	27.500135790494667	28.019243452958293	23.54808923375364
40	23.368845780795343	28.386886517943744	24.40054316197866	23.84372453928225
41	20.794568380213384	24.39914645974782	28.609117361784676	26.197167798254124
42	23.31002909796314	26.553792434529583	24.65132880698351	25.48484966052376
43	19.551193016488845	24.862230843840933	28.111425800193985	27.475150339476233
44	21.09113482056256	23.65516973811833	27.513637245392825	27.740058195926288
45	22.823821532492726	26.469524733268674	26.477129000969935	24.22952473326867
46	19.53055286129971	26.487837051406405	30.302541222114453	23.67906886517944
47	20.801862269641124	26.668787584869058	26.380756547041706	26.148593598448112
48	21.195887487875847	23.34556741028128	32.737148399612025	22.721396702230845
49	21.537458777885547	22.961474296799224	29.88259941804074	25.618467507274488
50	19.7380407371484	23.63235693501455	27.96306498545102	28.666537342386032
51	19.52496605237633	23.362638215324928	31.91899127061106	25.19340446168768
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4465.0
1	3507.5
2	2550.0
3	1746.0
4	942.0
5	1370.5
6	1799.0
7	1608.0
8	1417.0
9	1293.5
10	1170.0
11	1183.0
12	1196.0
13	1178.5
14	1161.0
15	1470.0
16	1779.0
17	1664.5
18	1550.0
19	1774.5
20	1999.0
21	2037.0
22	2075.0
23	2500.5
24	2926.0
25	3401.5
26	4801.5
27	5726.0
28	6264.0
29	6802.0
30	8479.5
31	10157.0
32	11096.5
33	12036.0
34	13512.5
35	14989.0
36	15988.0
37	16987.0
38	19648.5
39	22310.0
40	23750.0
41	25190.0
42	30434.0
43	35678.0
44	44497.5
45	53317.0
46	57117.0
47	60917.0
48	66457.5
49	71998.0
50	66681.5
51	61365.0
52	55278.5
53	49192.0
54	42899.5
55	36607.0
56	33525.0
57	30443.0
58	27198.0
59	23953.0
60	23023.5
61	22094.0
62	19471.5
63	16849.0
64	14951.5
65	13054.0
66	10507.0
67	7960.0
68	6661.0
69	5362.0
70	4730.0
71	4098.0
72	3773.5
73	3449.0
74	2724.0
75	1741.5
76	1484.0
77	1032.0
78	580.0
79	467.5
80	355.0
81	313.0
82	271.0
83	182.5
84	94.0
85	60.0
86	26.0
87	31.0
88	36.0
89	31.0
90	26.0
91	31.0
92	36.0
93	30.0
94	24.0
95	14.5
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	644375.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.27134076504574
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.72711932437274	25.86085473726577
2	9.458491135657928	6.293933633952789
3	3.3954305238638	3.3891157801053082
4	1.6268581268258049	2.165110044560214
5	0.9575736919521164	1.59298803062909
6	0.6552229893326671	1.3080088413107456
7	0.4734579128916726	1.1026805690408328
8	0.3927151506786169	1.0452927681459636
9	0.2854832934323914	0.8548570744664976
>10	4.258436163117954	33.760563179883505
>50	0.7070201045996317	15.041820176173124
>100	0.05377467726758715	3.3808817914960305
>500	0.004676058892833665	1.0237081675046653
>1k	0.0032732412249835657	2.0859998647268316
>5k	4.6760588928336657E-4	1.0941853407386493
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGCT	7033	1.0914451988360816	Illumina Single End Adapter 1 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3888	0.6033753637245393	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCC	2028	0.3147235693501455	No Hit
AATCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCT	1871	0.2903588748787585	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGT	1804	0.27996120271580993	No Hit
AAACTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCT	1695	0.26304558680892337	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTT	1072	0.1663627546071775	No Hit
CCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGC	1050	0.1629485935984481	Illumina Single End Adapter 1 (95% over 21bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	883	0.13703200775945684	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	839	0.13020368574199806	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	832	0.1291173617846751	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	704	0.10925315227934043	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.655674102812803E-4	0.0	0.0	1.3431619786614937	0.0
2	4.655674102812803E-4	0.0	0.0	1.7668283220174588	0.0
3	4.655674102812803E-4	0.0	0.0	2.3054898157129	0.0
4	4.655674102812803E-4	0.0	0.0	4.74164888457808	0.0
5	4.655674102812803E-4	0.0	0.0	5.053579049466538	0.0
6	4.655674102812803E-4	0.0	0.0	6.859980601357905	0.0
7	4.655674102812803E-4	0.0	0.0	8.172570320077595	0.0
8	4.655674102812803E-4	0.0	0.0	8.946032977691562	0.0
9	6.20756547041707E-4	0.0	0.0	10.43662463627546	0.0
10	6.20756547041707E-4	0.0	0.0	12.128341416100874	0.0
11	6.20756547041707E-4	0.0	0.0	14.299127061105722	0.0
12	6.20756547041707E-4	0.0	0.0	15.214122211445199	0.0
13	6.20756547041707E-4	0.0	0.0	15.569970902036857	0.0
14	6.20756547041707E-4	0.0	0.0	16.02172647914646	0.0
15	6.20756547041707E-4	0.0	0.0	16.37943743937924	0.0
16	6.20756547041707E-4	0.0	0.0	17.106653734238602	0.0
17	6.20756547041707E-4	0.0	0.0	17.93800193986421	0.0
18	6.20756547041707E-4	0.0	0.0	19.009582929194956	0.0
19	6.20756547041707E-4	0.0	0.0	19.50168768186227	0.0
20	6.20756547041707E-4	0.0	0.0	20.02917555771096	0.0
21	6.20756547041707E-4	0.0	0.0	20.61858389912706	0.0
22	6.20756547041707E-4	0.0	0.0	21.204578079534432	0.0
23	6.20756547041707E-4	0.0	0.0	21.82952473326867	0.0
24	6.20756547041707E-4	0.0	0.0	22.276003879728417	0.0
25	6.20756547041707E-4	0.0	0.0	22.665683802133852	0.0
26	6.20756547041707E-4	0.0	0.0	23.0184287099903	0.0
27	6.20756547041707E-4	0.0	0.0	23.38653734238603	0.0
28	6.20756547041707E-4	0.0	0.0	23.761939864209506	0.0
29	6.20756547041707E-4	0.0	0.0	24.144015518913676	0.0
30	6.20756547041707E-4	0.0	0.0	24.61051406401552	0.0
31	6.20756547041707E-4	0.0	0.0	24.984985451018428	0.0
32	6.20756547041707E-4	0.0	0.0	25.348128031037827	0.0
33	6.20756547041707E-4	0.0	0.0	25.739825412221144	0.0
34	6.20756547041707E-4	0.0	0.0	26.113365664403492	0.0
35	6.20756547041707E-4	0.0	0.0	26.52290979631426	0.0
36	6.20756547041707E-4	0.0	0.0	26.874568380213386	0.0
37	6.20756547041707E-4	0.0	0.0	27.250436469447138	0.0
38	6.20756547041707E-4	0.0	0.0	27.630029097963142	0.0
39	6.20756547041707E-4	0.0	0.0	28.05074684772066	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTACAG	30	2.1640099E-6	45.000004	1
TCCCGCG	30	2.1640099E-6	45.000004	15
GTTCACG	30	2.1640099E-6	45.000004	1
CGAGACG	30	2.1640099E-6	45.000004	1
ATGCACG	30	2.1640099E-6	45.000004	1
GTACCTA	20	7.030978E-4	45.0	21
TACCCCG	25	3.8888917E-5	45.0	1
CTATCCG	20	7.030978E-4	45.0	1
ATTCGCT	20	7.030978E-4	45.0	23
CGATTGA	20	7.030978E-4	45.0	15
TAACGCG	20	7.030978E-4	45.0	1
TAGCCGT	20	7.030978E-4	45.0	44
AGTATTC	25	3.8888917E-5	45.0	19
ATCGTAG	20	7.030978E-4	45.0	12
GTAATCG	20	7.030978E-4	45.0	35
AATGCGC	20	7.030978E-4	45.0	34
ACGATAG	40	6.8066583E-9	45.0	1
ATTCACG	35	1.2108103E-7	45.0	1
CTAAACG	20	7.030978E-4	45.0	1
ACGTGTA	25	3.8888917E-5	45.0	28
>>END_MODULE