##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552879_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	321395
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.033998662082485	31.0	31.0	33.0	28.0	34.0
2	31.303841067844864	31.0	31.0	34.0	28.0	34.0
3	31.52164159367756	31.0	31.0	34.0	30.0	34.0
4	35.44958695685994	37.0	35.0	37.0	33.0	37.0
5	34.81448062353179	35.0	35.0	37.0	32.0	37.0
6	34.93062430965012	35.0	35.0	37.0	32.0	37.0
7	34.80508719799624	36.0	35.0	37.0	32.0	37.0
8	35.30523499121019	37.0	35.0	37.0	33.0	37.0
9	36.720070318455484	39.0	37.0	39.0	32.0	39.0
10	36.189791378210614	38.0	35.0	39.0	32.0	39.0
11	36.37065915773425	38.0	35.0	39.0	32.0	39.0
12	36.053980304609595	37.0	35.0	39.0	32.0	39.0
13	35.702842296862116	37.0	35.0	39.0	30.0	39.0
14	36.745061373076744	39.0	35.0	40.0	31.0	41.0
15	36.77950185908306	38.0	35.0	40.0	31.0	41.0
16	36.677764122030524	38.0	35.0	40.0	31.0	41.0
17	36.581598967003224	38.0	35.0	40.0	31.0	41.0
18	36.463806219760734	38.0	35.0	40.0	31.0	41.0
19	36.52870144215063	38.0	35.0	40.0	31.0	41.0
20	36.37292428320291	38.0	35.0	40.0	30.0	41.0
21	36.5096003360351	38.0	35.0	40.0	31.0	41.0
22	36.44594346520637	38.0	35.0	40.0	30.0	41.0
23	36.35119712503306	38.0	35.0	40.0	30.0	41.0
24	35.84288492353646	38.0	34.0	40.0	29.0	41.0
25	36.07357612906237	38.0	34.0	40.0	30.0	41.0
26	35.959775354314786	38.0	34.0	40.0	30.0	41.0
27	35.869506370665384	38.0	34.0	40.0	29.0	41.0
28	35.939043855691594	38.0	34.0	40.0	30.0	41.0
29	35.475679459854696	38.0	34.0	40.0	27.0	41.0
30	35.562893013270276	38.0	34.0	40.0	28.0	41.0
31	35.43447471180323	38.0	34.0	40.0	27.0	41.0
32	35.397065915773425	38.0	34.0	40.0	27.0	41.0
33	35.3315421832947	38.0	34.0	40.0	27.0	41.0
34	35.198711865461505	38.0	34.0	40.0	26.0	41.0
35	35.198562516529506	38.0	34.0	40.0	26.0	41.0
36	35.13873271208326	38.0	34.0	40.0	26.0	41.0
37	35.009570777392305	38.0	34.0	40.0	26.0	41.0
38	34.94648018793074	37.0	34.0	40.0	25.0	41.0
39	34.826294746340174	37.0	34.0	40.0	25.0	41.0
40	34.60161172389116	37.0	33.0	40.0	24.0	41.0
41	34.65614897555967	37.0	33.0	40.0	25.0	41.0
42	34.62424430996126	37.0	33.0	40.0	25.0	41.0
43	34.635078330403395	37.0	33.0	40.0	25.0	41.0
44	34.505452791736026	37.0	33.0	40.0	24.0	41.0
45	34.32713016692855	37.0	33.0	40.0	24.0	41.0
46	34.32510151060222	37.0	33.0	40.0	24.0	41.0
47	34.16673563683318	36.0	33.0	40.0	23.0	41.0
48	34.19525194853685	36.0	33.0	40.0	23.0	41.0
49	34.09071391900932	36.0	33.0	40.0	24.0	41.0
50	33.979592090729476	36.0	33.0	40.0	23.0	41.0
51	32.770351125562	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	8.0
11	15.0
12	13.0
13	20.0
14	42.0
15	59.0
16	91.0
17	230.0
18	448.0
19	765.0
20	1133.0
21	1722.0
22	2281.0
23	2704.0
24	3124.0
25	3303.0
26	3682.0
27	4146.0
28	5015.0
29	6365.0
30	8084.0
31	10459.0
32	14077.0
33	19598.0
34	30554.0
35	31776.0
36	34850.0
37	46844.0
38	56426.0
39	33556.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.38208435103222	36.64711647660978	16.613512966909877	26.357286205448126
2	21.56971950403709	35.79769442586226	17.82355045971468	24.809035610385973
3	21.10207066071345	32.72857387327121	21.33387264892111	24.835482817094228
4	22.064748984893978	31.82470169106551	19.092394094494313	27.0181552295462
5	17.6913144261734	36.49341153409356	18.87490471226995	26.94036932746309
6	20.233979993465983	35.453880738654924	22.09430762768556	22.21783164019353
7	73.95821341340096	12.252524152522597	8.045240280651536	5.744022153424913
8	76.16795531977785	8.862614539740818	6.444717559389536	8.524712581091803
9	71.71580142814916	11.627436643382753	10.619642495994027	6.037119432474059
10	37.67544610214845	31.754694379190717	14.209617448933557	16.36024206972728
11	29.70145770780504	27.394016708411765	23.909830582305265	18.994695001477933
12	29.529084148788876	24.60430311610324	28.51537827284183	17.35123446226606
13	23.979526750571726	32.02414474400659	23.643491653572706	20.35283685184897
14	17.20655268439148	33.369218562827676	29.04650041226528	20.377728340515567
15	16.01705066973662	31.4522627918916	30.00948988005414	22.521196658317646
16	17.721184212573313	28.22321442461768	31.6902876522659	22.3653137105431
17	16.82664633861759	29.515393830022248	26.618024549230697	27.03993528212947
18	20.790927052381026	29.564243376530435	25.677748564850106	23.96708100623843
19	21.375254748829324	32.97468846746216	25.57538231770874	20.07467446599978
20	23.799996888563918	31.293890695250393	23.93409978375519	20.9720126324305
21	21.015883881205372	30.79046033696853	25.607118965758644	22.586536816067458
22	19.95550646400846	30.457536676052833	25.51813189377557	24.06882496616313
23	21.596477854353676	30.749700524276978	22.40482894880132	25.248992672568022
24	19.22307440999393	32.51015106022185	26.034319139999067	22.232455389785155
25	19.3599775976602	30.261205059195074	25.273884161234616	25.10493318191011
26	21.473264985454037	32.762799670187775	22.865943776349976	22.897991568008212
27	18.700975435212122	30.878825121734938	26.61055710263072	23.809642340422222
28	19.95768446926679	28.70362015588295	28.27050825308421	23.06818712176605
29	19.28156940836043	29.855162650321255	29.017252913082032	21.84601502823628
30	21.59834471600367	29.096905676815133	25.06759594891022	24.237153658270973
31	23.127615550957543	30.24813702764511	22.44309961262621	24.18114780877114
32	23.75830364504737	27.815616297702206	25.47892779912569	22.94715225812474
33	22.33295477527653	28.537469469033432	22.48572628696775	26.64384946872229
34	18.73520123212869	30.00855644922914	26.907076961371523	24.34916535727065
35	21.008105290997058	26.225361315515176	27.978966692076728	24.787566701411038
36	22.77166726302525	27.024689245321177	27.8962024922603	22.30744099939327
37	20.620420355014858	29.16846870673159	27.043669005429454	23.167441932824097
38	21.600522721261996	27.463401733069897	26.904898956113193	24.03117658955491
39	20.570015090465006	25.862879011807898	29.236609156956394	24.330496740770702
40	22.39238320446802	27.64977675446102	25.563247716983774	24.394592324087185
41	19.035765957777812	25.351981206926055	31.097559078392635	24.5146937569035
42	23.488853280231492	25.818696619424696	26.086902409807244	24.605547690536568
43	21.876818245461195	25.58316090791705	27.86477698781873	24.675243858803032
44	22.74210862023367	25.906127973366104	25.68708287310008	25.664680533300142
45	23.086544594657667	27.36756950170351	26.057032623407334	23.488853280231492
46	20.381150920207222	26.29256833491498	30.913050918651503	22.413229826226296
47	22.018388587252446	27.413307612128378	26.422937506806264	24.14536629381291
48	22.165559513993685	24.34418705953733	31.450707073849937	22.039546352619052
49	21.626969927970254	23.510322189206427	29.515393830022248	25.347314052801067
50	19.750462826117392	24.833615955444234	27.83397377059382	27.581947447844552
51	19.448031238818274	25.10213288943512	30.337435243236516	25.11240062851009
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4273.0
1	3365.5
2	2458.0
3	1677.5
4	897.0
5	1196.5
6	1496.0
7	1332.5
8	1169.0
9	1076.5
10	984.0
11	1025.5
12	1067.0
13	1099.5
14	1132.0
15	1141.5
16	1151.0
17	1180.5
18	1210.0
19	1195.0
20	1180.0
21	1252.0
22	1324.0
23	1652.5
24	1981.0
25	2098.5
26	2588.0
27	2960.0
28	3309.5
29	3659.0
30	4320.0
31	4981.0
32	5735.0
33	6489.0
34	7003.0
35	7517.0
36	7929.0
37	8341.0
38	9361.5
39	10382.0
40	11058.0
41	11734.0
42	14567.0
43	17400.0
44	22651.5
45	27903.0
46	28001.0
47	28099.0
48	29104.0
49	30109.0
50	28511.5
51	26914.0
52	24462.5
53	22011.0
54	20230.5
55	18450.0
56	16703.0
57	14956.0
58	13563.5
59	12171.0
60	11812.5
61	11454.0
62	10614.0
63	9774.0
64	8277.0
65	6780.0
66	5860.0
67	4940.0
68	4256.0
69	3572.0
70	3179.0
71	2786.0
72	2250.0
73	1714.0
74	1562.5
75	1235.0
76	1059.0
77	932.5
78	806.0
79	527.5
80	249.0
81	194.0
82	139.0
83	95.0
84	51.0
85	37.5
86	24.0
87	16.5
88	9.0
89	8.5
90	8.0
91	6.5
92	5.0
93	2.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	321395.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.8778306473272
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.20995909673715	25.056181288222568
2	9.354899063120932	6.151375742402598
3	3.35700325647308	3.311129536465435
4	1.6146582854638405	2.123458466511354
5	1.0580584559329986	1.739333336456882
6	0.7458358738514108	1.4712879330712787
7	0.5972545948030683	1.374550479489132
8	0.5144946241012255	1.3532373696128288
9	0.43273358858380045	1.2804607476781515
>10	5.74817703023504	41.77461693859754
>50	0.31009246371443294	6.441313387310261
>100	0.04785993009097357	3.173942501701764
>500	0.003988327507581131	0.9398567602126582
>1k	0.004985409384476414	3.809255512267558
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3627	1.1285178674217085	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCC	2136	0.6646027473980616	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGT	2069	0.643756125639789	No Hit
AATCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCT	1985	0.6176200625398652	No Hit
CTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCTGCT	1803	0.5609919258233638	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	834	0.2594937693492431	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	682	0.21219994088271443	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	679	0.21126651005771713	No Hit
AAACTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCT	672	0.20908850479939017	No Hit
AATGACTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTT	482	0.14997121921622925	No Hit
AACTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCTG	454	0.14125919818292135	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTT	437	0.13596975684127008	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCT	419	0.1303691718912864	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	415	0.1291245974579567	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCTGCTCATTCGTAT	391	0.12165715085797849	No Hit
CCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCTGC	364	0.11325627343300301	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTGCTCATTC	344	0.1070334012663545	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	332	0.10329967796636537	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.222872166648517E-4	0.0	0.0	0.7305651923645359	0.0
2	6.222872166648517E-4	0.0	0.0	1.038286221005305	0.0
3	6.222872166648517E-4	0.0	0.0	1.5550957544454642	0.0
4	6.222872166648517E-4	0.0	0.0	3.7822617028889685	0.0
5	6.222872166648517E-4	0.0	0.0	4.08780472627141	0.0
6	6.222872166648517E-4	0.0	0.0	5.514709314083915	0.0
7	6.222872166648517E-4	0.0	0.0	6.532148913330948	0.0
8	6.222872166648517E-4	0.0	0.0	7.046469297904448	0.0
9	6.222872166648517E-4	0.0	0.0	8.629879120708162	0.0
10	6.222872166648517E-4	0.0	0.0	9.909301638171097	0.0
11	6.222872166648517E-4	0.0	0.0	12.099130353614711	0.0
12	6.222872166648517E-4	0.0	0.0	12.860498763204157	0.0
13	6.222872166648517E-4	0.0	0.0	13.205245881236484	0.0
14	6.222872166648517E-4	0.0	0.0	13.645514087026868	0.0
15	6.222872166648517E-4	0.0	0.0	13.937989078859347	0.0
16	6.222872166648517E-4	0.0	0.0	14.445775447657866	0.0
17	6.222872166648517E-4	0.0	0.0	15.046904898956113	0.0
18	6.222872166648517E-4	0.0	0.0	15.885748067020334	0.0
19	6.222872166648517E-4	0.0	0.0	16.25227523763593	0.0
20	6.222872166648517E-4	0.0	0.0	16.636537593926477	0.0
21	6.222872166648517E-4	0.0	0.0	17.031067689291994	0.0
22	9.334308249972775E-4	0.0	0.0	17.46137929961574	0.0
23	9.334308249972775E-4	0.0	0.0	17.854975964156257	0.0
24	9.334308249972775E-4	0.0	0.0	18.12100374928048	0.0
25	9.334308249972775E-4	0.0	0.0	18.370540923163087	0.0
26	9.334308249972775E-4	0.0	0.0	18.584918869304126	0.0
27	9.334308249972775E-4	0.0	0.0	18.84036777174505	0.0
28	9.334308249972775E-4	0.0	0.0	19.083993217069338	0.0
29	9.334308249972775E-4	0.0	0.0	19.334152678168607	0.0
30	9.334308249972775E-4	0.0	0.0	19.681388945067596	0.0
31	9.334308249972775E-4	0.0	0.0	19.923458672350222	0.0
32	9.334308249972775E-4	0.0	0.0	20.18481930334946	0.0
33	9.334308249972775E-4	0.0	0.0	20.44151278022371	0.0
34	9.334308249972775E-4	0.0	0.0	20.67207019399804	0.0
35	9.334308249972775E-4	0.0	0.0	20.930008245305622	0.0
36	9.334308249972775E-4	0.0	0.0	21.166788531246596	0.0
37	9.334308249972775E-4	0.0	0.0	21.398901663062585	0.0
38	9.334308249972775E-4	0.0	0.0	21.64688311890353	0.0
39	0.0012445744333297033	0.0	0.0	21.895175718352807	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCCGG	35	1.2082819E-7	45.0	2
AGATCCG	20	7.025694E-4	45.0	17
CGACGGT	20	7.025694E-4	45.0	28
ACGTTAG	20	7.025694E-4	45.0	1
ATTACGG	35	1.2082819E-7	45.0	2
ACCGAAC	20	7.025694E-4	45.0	10
CATTACG	20	7.025694E-4	45.0	1
CCACTCG	20	7.025694E-4	45.0	31
TAACATA	20	7.025694E-4	45.0	14
CGCTACG	20	7.025694E-4	45.0	1
CCATATG	20	7.025694E-4	45.0	1
ACGCTAT	20	7.025694E-4	45.0	37
TACTCGA	20	7.025694E-4	45.0	41
ACATGCG	20	7.025694E-4	45.0	1
ACGTCGA	20	7.025694E-4	45.0	28
CCGAACT	20	7.025694E-4	45.0	11
GTGACGT	20	7.025694E-4	45.0	44
ATCGCTA	20	7.025694E-4	45.0	28
GGGTCTA	25	3.8845144E-5	44.999996	8
CGTGAAT	25	3.8845144E-5	44.999996	27
>>END_MODULE