FastQCFastQC Report
Sat 18 Jun 2016
SRR3552878_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552878_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences325910
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36041.1058267619895061No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCC22450.68884047743242No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGT21590.662452824399374No Hit
AATCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCT21560.6615323248749655No Hit
CTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCTGCT18440.5658003743364732No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8940.27430885827375656No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6540.2006688963210702No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6290.19299806695099875No Hit
AAACTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCT6150.1887024025037587No Hit
AATGACTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTT5300.16262158264551563No Hit
AATGATCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCT5000.15341658740142985No Hit
AAAAACTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTT4410.13531343008806113No Hit
AACTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCTG4340.1331655978644411No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4030.12365376944555245No Hit
CCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCTGC3850.11813077229910099No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTGCTCATTC3580.10984627657942377No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCTGCTCATTCGTAT3550.10892577705501519No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA3430.10524377895738087No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCAG253.8846345E-545.025
CTTCGAC207.0258405E-445.013
ATCTACG253.8846345E-545.01
CGCATAG253.8846345E-545.01
TAGAACG207.0258405E-445.01
CCTCGCA253.8846345E-545.037
TAGCCGT253.8846345E-545.044
CCGTCAA207.0258405E-445.013
GTTACCG253.8846345E-545.01
CTCGGGT207.0258405E-445.04
AGGCACG207.0258405E-445.01
TGCGACG207.0258405E-445.01
CCATACG207.0258405E-445.01
CATACGA800.045.018
CTCGCGG453.8380676E-1045.02
TATAGTT207.0258405E-445.023
TTCGGTC207.0258405E-445.034
ACGTATA207.0258405E-445.013
ACCAACG207.0258405E-445.01
ATCGGTC253.8846345E-545.025