##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552878_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	325910
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.144911171795894	31.0	31.0	34.0	28.0	34.0
2	31.408594397226228	31.0	31.0	34.0	30.0	34.0
3	31.63484704366236	31.0	31.0	34.0	30.0	34.0
4	35.52375195606149	37.0	35.0	37.0	33.0	37.0
5	34.873176643858734	35.0	35.0	37.0	32.0	37.0
6	34.98491608112669	36.0	35.0	37.0	32.0	37.0
7	34.874391703230955	36.0	35.0	37.0	32.0	37.0
8	35.316421711515446	37.0	35.0	37.0	33.0	37.0
9	36.80906385198367	39.0	37.0	39.0	32.0	39.0
10	36.26681906047682	38.0	35.0	39.0	32.0	39.0
11	36.40775674265902	38.0	35.0	39.0	32.0	39.0
12	36.057957718388515	37.0	35.0	39.0	32.0	39.0
13	35.78813782946212	37.0	35.0	39.0	30.0	39.0
14	36.80820778742598	39.0	35.0	40.0	31.0	41.0
15	36.82928722653493	38.0	35.0	40.0	31.0	41.0
16	36.74215274155441	38.0	35.0	40.0	31.0	41.0
17	36.646331809395235	38.0	35.0	40.0	31.0	41.0
18	36.49760363290479	38.0	35.0	40.0	31.0	41.0
19	36.5838943266546	38.0	35.0	40.0	31.0	41.0
20	36.41547052867356	38.0	35.0	40.0	30.0	41.0
21	36.51526801877819	38.0	35.0	40.0	31.0	41.0
22	36.495400570709705	38.0	35.0	40.0	31.0	41.0
23	36.36192507133871	38.0	35.0	40.0	30.0	41.0
24	35.876426007179894	38.0	34.0	40.0	29.0	41.0
25	36.065515019483904	38.0	34.0	40.0	30.0	41.0
26	35.92165321714584	38.0	34.0	40.0	30.0	41.0
27	35.787122211653525	38.0	34.0	40.0	29.0	41.0
28	35.807026479702984	38.0	34.0	40.0	29.0	41.0
29	35.459387560983096	38.0	34.0	40.0	27.0	41.0
30	35.55966677917216	38.0	34.0	40.0	28.0	41.0
31	35.425132705348105	38.0	34.0	40.0	27.0	41.0
32	35.391792212574025	38.0	34.0	40.0	27.0	41.0
33	35.30908533030591	38.0	34.0	40.0	27.0	41.0
34	35.12860605688687	38.0	34.0	40.0	26.0	41.0
35	35.13697646589549	38.0	34.0	40.0	26.0	41.0
36	35.08531496425393	38.0	34.0	40.0	26.0	41.0
37	34.93842471848056	38.0	34.0	40.0	25.0	41.0
38	34.92293884814826	37.0	34.0	40.0	26.0	41.0
39	34.82757509741953	37.0	34.0	40.0	25.0	41.0
40	34.54829247338222	37.0	33.0	40.0	24.0	41.0
41	34.594032094750084	37.0	33.0	40.0	24.0	41.0
42	34.59339081341474	37.0	33.0	40.0	25.0	41.0
43	34.63098401399159	37.0	33.0	40.0	25.0	41.0
44	34.454564143475196	37.0	33.0	40.0	24.0	41.0
45	34.264026878586115	37.0	33.0	40.0	23.0	41.0
46	34.24403056058421	36.0	33.0	40.0	24.0	41.0
47	34.09421619465497	36.0	33.0	40.0	23.0	41.0
48	34.107759810990764	36.0	33.0	40.0	23.0	41.0
49	33.99756067626032	36.0	33.0	39.0	24.0	41.0
50	33.90597404191341	36.0	33.0	39.0	23.0	41.0
51	32.60868337884692	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	6.0
11	12.0
12	10.0
13	18.0
14	26.0
15	59.0
16	121.0
17	186.0
18	374.0
19	737.0
20	1235.0
21	1775.0
22	2269.0
23	2708.0
24	3060.0
25	3418.0
26	3813.0
27	4415.0
28	5168.0
29	6489.0
30	8089.0
31	10727.0
32	14316.0
33	19814.0
34	30568.0
35	32715.0
36	35107.0
37	47350.0
38	57119.0
39	34200.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.293946181461138	36.7810131631432	16.656438894173238	26.26860176122242
2	21.51575588352613	35.90684544812986	17.690773526433677	24.886625141910343
3	21.02328863796754	32.65993679236599	21.578656684360713	24.738117885305762
4	22.12880856678224	31.824736890552607	19.111411125770918	26.93504341689423
5	17.775152649504463	36.57942376729772	18.785861127305083	26.859562455892732
6	20.237795710472216	35.50888282041054	21.933049001258016	22.320272467859226
7	73.97441011322144	12.346046454542666	8.010186861403454	5.669356570832439
8	76.2876254180602	8.873615415298703	6.3385597250774754	8.500199441563622
9	71.70783345095272	11.64278481789451	10.677487649964714	5.971894081188058
10	37.55484642999601	31.855420208032893	14.223558651161364	16.366174710809734
11	29.81835476051671	27.346199871130068	24.015525758645026	18.8199196097082
12	29.439415789635177	24.678899082568808	28.619250713387128	17.262434414408887
13	24.028412751986743	32.108864410420054	23.58810714614464	20.27461569144856
14	17.221932435334907	33.24537448988985	29.249792887607008	20.282900187168238
15	15.941824430057377	31.595225675800066	29.868675401184376	22.594274492958178
16	17.651805713233713	28.309656040011046	31.718265778896015	22.320272467859226
17	16.82734497253843	29.82725292258599	26.610720751127616	26.73468135374797
18	20.699272805375717	29.83983308275291	25.45825534656807	24.002638765303306
19	21.280721671627138	32.94958730937989	25.643582584148998	20.126108434843974
20	23.91089564603725	31.446411586020677	23.810561197876716	20.832131570065354
21	20.98063882666994	30.84655272928109	25.490165996747567	22.6826424473014
22	19.660642508668037	30.67472615139149	25.64787824859624	24.01675309134424
23	21.725936608266085	30.63851983676475	22.225767850019945	25.409775704949215
24	19.15682243564174	32.68724494492344	25.93139210211408	22.224540517320733
25	19.471326439814675	30.226442883004513	25.11061335951643	25.191617317664385
26	21.643398484244116	32.79248872388082	22.73480408701789	22.82930870485717
27	18.673253352152436	30.86588321929367	26.559172777760732	23.901690650793164
28	20.0214783222362	28.618637047037527	28.08812248780338	23.271762142922892
29	19.21051824123224	30.004295664447238	28.986836856800956	21.79834923751956
30	21.76398392194164	28.940505047405722	24.93234328495597	24.363167745696664
31	23.133687214261606	30.285968518916267	22.337148292473383	24.243195974348748
32	23.780798379920835	27.774538983154855	25.47727900340585	22.967383633518455
33	22.463870393666962	28.359976680678717	22.537817188794453	26.63833573685987
34	18.614034549415486	30.12549476849437	26.875824614157285	24.384646067932863
35	21.0407781289313	26.222576785001994	27.846031112883924	24.89061397318278
36	22.73081525574545	26.93749808229266	28.076155993986067	22.25553066797582
37	20.792243257340985	29.248872388082596	26.90896259703599	23.049921757540424
38	21.770120585437695	27.415237335460713	26.83286796968488	23.98177410941671
39	20.52990089288454	26.114264674296585	29.346752170844713	24.009082261974164
40	22.543340185940906	27.72851400693443	25.3434997391918	24.384646067932863
41	19.100365131478014	25.401491209229544	31.03556196495965	24.46258169433279
42	23.486852198459697	25.776134515663834	25.99337240342426	24.743640882452212
43	21.921696173790313	25.52913380994753	27.770856985057225	24.778313031204934
44	22.75536190972968	26.012396060262034	25.658617409714342	25.573624620293945
45	23.24997698751189	27.617133564480987	25.771225184866985	23.36166426314013
46	20.28535485256666	26.23822527691694	30.989843821914025	22.486576048602373
47	21.943788162376116	27.50115062440551	26.36402687858611	24.19103433463226
48	22.25737166702464	24.210057991470038	31.471265073179712	22.06130526832561
49	21.64922831456537	23.614494799177688	29.63977785278144	25.0964990334755
50	19.729373139823878	24.703445736553036	27.759197324414714	27.80798379920837
51	19.428369795342274	25.026541069620446	30.364210978490995	25.18087815654629
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4240.0
1	3403.0
2	2566.0
3	1723.0
4	880.0
5	1159.5
6	1439.0
7	1291.5
8	1144.0
9	1063.5
10	983.0
11	1013.5
12	1044.0
13	1096.0
14	1148.0
15	1158.0
16	1168.0
17	1200.5
18	1233.0
19	1237.0
20	1241.0
21	1305.5
22	1370.0
23	1720.0
24	2070.0
25	2173.5
26	2620.0
27	2963.0
28	3322.0
29	3681.0
30	4348.5
31	5016.0
32	5811.0
33	6606.0
34	7194.0
35	7782.0
36	8158.0
37	8534.0
38	9492.0
39	10450.0
40	11178.0
41	11906.0
42	14779.5
43	17653.0
44	23132.5
45	28612.0
46	28711.0
47	28810.0
48	29446.0
49	30082.0
50	28746.0
51	27410.0
52	24821.0
53	22232.0
54	20485.0
55	18738.0
56	16699.0
57	14660.0
58	13540.5
59	12421.0
60	12012.5
61	11604.0
62	10806.0
63	10008.0
64	8580.0
65	7152.0
66	6071.0
67	4990.0
68	4301.5
69	3613.0
70	3141.5
71	2670.0
72	2210.0
73	1750.0
74	1616.5
75	1235.5
76	988.0
77	899.5
78	811.0
79	529.5
80	248.0
81	193.0
82	138.0
83	89.5
84	41.0
85	35.0
86	29.0
87	19.5
88	10.0
89	8.5
90	7.0
91	8.0
92	9.0
93	4.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	325910.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.43498679410386
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.1743188105063	24.707130246686287
2	9.362320891953233	6.073335089853314
3	3.3214324628851433	3.2319185421356234
4	1.6165839935997788	2.0973552193587395
5	1.0440044695475559	1.6931135591380184
6	0.7337981002774185	1.428043901522194
7	0.6011823081294035	1.3649538157518253
8	0.5224183794567862	1.3555706590942396
9	0.46758406766832855	1.3649474753960036
>10	5.720675901877597	40.99013318595372
>50	0.371873842239629	7.478915101699633
>100	0.05383696375587122	3.257311994959374
>500	0.004984904051469557	1.074237113794802
>1k	0.004984904051469557	3.883034094656226
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3604	1.1058267619895061	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCC	2245	0.68884047743242	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGT	2159	0.662452824399374	No Hit
AATCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCT	2156	0.6615323248749655	No Hit
CTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCTGCT	1844	0.5658003743364732	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	894	0.27430885827375656	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	654	0.2006688963210702	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	629	0.19299806695099875	No Hit
AAACTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCT	615	0.1887024025037587	No Hit
AATGACTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTT	530	0.16262158264551563	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCT	500	0.15341658740142985	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTT	441	0.13531343008806113	No Hit
AACTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCTG	434	0.1331655978644411	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	403	0.12365376944555245	No Hit
CCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCTGC	385	0.11813077229910099	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTGCTCATTC	358	0.10984627657942377	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCTGCTCATTCGTAT	355	0.10892577705501519	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	343	0.10524377895738087	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0015341658740142985	0.0	0.0	0.7600257739866835	0.0
2	0.0015341658740142985	0.0	0.0	1.086803105151729	0.0
3	0.0015341658740142985	0.0	0.0	1.6019760056457304	0.0
4	0.0015341658740142985	0.0	0.0	3.8940198214230923	0.0
5	0.0015341658740142985	0.0	0.0	4.191954834156669	0.0
6	0.0015341658740142985	0.0	0.0	5.643275750974196	0.0
7	0.0015341658740142985	0.0	0.0	6.698781872296033	0.0
8	0.0015341658740142985	0.0	0.0	7.219784603111289	0.0
9	0.0015341658740142985	0.0	0.0	8.837409100671964	0.0
10	0.0015341658740142985	0.0	0.0	10.097266116412506	0.0
11	0.0015341658740142985	0.0	0.0	12.33377312755055	0.0
12	0.0015341658740142985	0.0	0.0	13.06986591390261	0.0
13	0.0015341658740142985	0.0	0.0	13.4119849038078	0.0
14	0.0015341658740142985	0.0	0.0	13.852290509649903	0.0
15	0.0015341658740142985	0.0	0.0	14.13733852904176	0.0
16	0.0015341658740142985	0.0	0.0	14.618452947132644	0.0
17	0.0015341658740142985	0.0	0.0	15.195912982111626	0.0
18	0.0015341658740142985	0.0	0.0	16.051363873461998	0.0
19	0.0015341658740142985	0.0	0.0	16.425700346721488	0.0
20	0.0015341658740142985	0.0	0.0	16.79420698965972	0.0
21	0.0015341658740142985	0.0	0.0	17.197078948175875	0.0
22	0.0015341658740142985	0.0	0.0	17.590439078273143	0.0
23	0.0015341658740142985	0.0	0.0	17.977662544874352	0.0
24	0.0015341658740142985	0.0	0.0	18.250437237274095	0.0
25	0.0015341658740142985	0.0	0.0	18.506336105059678	0.0
26	0.0015341658740142985	0.0	0.0	18.734006320763402	0.0
27	0.0015341658740142985	0.0	0.0	18.993280353471818	0.0
28	0.0015341658740142985	0.0	0.0	19.22309840139916	0.0
29	0.0015341658740142985	0.0	0.0	19.50323708999417	0.0
30	0.0015341658740142985	0.0	0.0	19.851492743395415	0.0
31	0.0015341658740142985	0.0	0.0	20.0966524500629	0.0
32	0.0015341658740142985	0.0	0.0	20.353778650547696	0.0
33	0.0015341658740142985	0.0	0.0	20.590960694670308	0.0
34	0.0015341658740142985	0.0	0.0	20.841950231659048	0.0
35	0.0015341658740142985	0.0	0.0	21.09999693166825	0.0
36	0.0015341658740142985	0.0	0.0	21.340247307538892	0.0
37	0.0015341658740142985	0.0	0.0	21.56883802276702	0.0
38	0.0015341658740142985	0.0	0.0	21.80571323371483	0.0
39	0.0015341658740142985	0.0	0.0	22.053020772605933	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCAG	25	3.8846345E-5	45.0	25
CTTCGAC	20	7.0258405E-4	45.0	13
ATCTACG	25	3.8846345E-5	45.0	1
CGCATAG	25	3.8846345E-5	45.0	1
TAGAACG	20	7.0258405E-4	45.0	1
CCTCGCA	25	3.8846345E-5	45.0	37
TAGCCGT	25	3.8846345E-5	45.0	44
CCGTCAA	20	7.0258405E-4	45.0	13
GTTACCG	25	3.8846345E-5	45.0	1
CTCGGGT	20	7.0258405E-4	45.0	4
AGGCACG	20	7.0258405E-4	45.0	1
TGCGACG	20	7.0258405E-4	45.0	1
CCATACG	20	7.0258405E-4	45.0	1
CATACGA	80	0.0	45.0	18
CTCGCGG	45	3.8380676E-10	45.0	2
TATAGTT	20	7.0258405E-4	45.0	23
TTCGGTC	20	7.0258405E-4	45.0	34
ACGTATA	20	7.0258405E-4	45.0	13
ACCAACG	20	7.0258405E-4	45.0	1
ATCGGTC	25	3.8846345E-5	45.0	25
>>END_MODULE