FastQCFastQC Report
Sat 18 Jun 2016
SRR3552877_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552877_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences635708
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGCT55840.8783907076834019TruSeq Adapter, Index 20 (95% over 22bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCC30980.4873306612469876No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGT28030.4409257080294726No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27460.43195932723829183No Hit
AATCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCT25970.4085208932402927No Hit
AAACTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCT10290.16186676901973862TruSeq Adapter, Index 27 (95% over 21bp)
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG9560.1503835094099807No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7830.12316975718411599No Hit
CCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGC7780.12238323255331063TruSeq Adapter, Index 27 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG351.2107739E-745.0000041
TCACGCG207.030905E-445.01
AACCCGC253.888832E-545.045
GACACGA253.888832E-545.025
CGCATCG253.888832E-545.021
AAGCGAC207.030905E-445.014
ATCGTAG253.888832E-545.01
CTAACCG253.888832E-545.01
TGACCGG406.8066583E-945.02
TATCTCG207.030905E-445.01
CGGGCTA406.8066583E-945.06
GAGCCGT207.030905E-445.023
CGTAACG453.8380676E-1045.01
TTACGAG207.030905E-445.01
AACTCGA207.030905E-445.011
ACTCCCG253.888832E-545.01
TCGACGG406.8066583E-945.02
GCATCGC406.8066583E-945.022
AATCGGC207.030905E-445.027
TATCCGG502.1827873E-1145.02