##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552877_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	635708
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.055262793609646	31.0	31.0	33.0	28.0	34.0
2	31.35210820062041	31.0	31.0	34.0	28.0	34.0
3	31.532274251700468	31.0	31.0	34.0	30.0	34.0
4	35.49078822352401	37.0	35.0	37.0	33.0	37.0
5	34.87394527047009	35.0	35.0	37.0	32.0	37.0
6	34.97541166699176	35.0	35.0	37.0	32.0	37.0
7	35.02319146526393	36.0	35.0	37.0	32.0	37.0
8	35.435122729303394	37.0	35.0	37.0	33.0	37.0
9	36.881332309802616	39.0	37.0	39.0	33.0	39.0
10	36.18155505357806	38.0	35.0	39.0	32.0	39.0
11	36.461711980972396	38.0	35.0	39.0	32.0	39.0
12	36.24848987270885	37.0	35.0	39.0	32.0	39.0
13	35.967782378072954	38.0	35.0	39.0	31.0	39.0
14	37.02097661190358	39.0	36.0	40.0	32.0	41.0
15	36.97610380866687	39.0	35.0	40.0	32.0	41.0
16	36.87257199846471	38.0	35.0	40.0	32.0	41.0
17	36.82133463791552	38.0	35.0	40.0	32.0	41.0
18	36.7534009325036	38.0	35.0	40.0	31.0	41.0
19	36.914813090286735	39.0	35.0	40.0	31.0	41.0
20	36.77646340772807	39.0	35.0	40.0	31.0	41.0
21	36.91906661549013	39.0	35.0	40.0	31.0	41.0
22	36.91392746355245	39.0	35.0	40.0	31.0	41.0
23	36.86178402662858	39.0	35.0	40.0	31.0	41.0
24	36.403141064765585	38.0	35.0	40.0	30.0	41.0
25	36.64535289787135	38.0	35.0	40.0	31.0	41.0
26	36.55759562566461	38.0	35.0	40.0	31.0	41.0
27	36.424896336053656	38.0	35.0	40.0	30.0	41.0
28	36.50303755812417	38.0	35.0	40.0	30.0	41.0
29	36.06421973610526	38.0	35.0	40.0	30.0	41.0
30	36.10078998533918	38.0	35.0	40.0	30.0	41.0
31	36.13536403506012	38.0	35.0	40.0	30.0	41.0
32	36.09710905006701	38.0	35.0	40.0	30.0	41.0
33	36.089268972547146	38.0	35.0	40.0	30.0	41.0
34	36.08009180315491	38.0	35.0	40.0	30.0	41.0
35	35.99807773380231	38.0	35.0	40.0	30.0	41.0
36	35.9757420073367	38.0	35.0	40.0	30.0	41.0
37	35.81747437502753	38.0	35.0	40.0	29.0	41.0
38	35.773583154530066	38.0	34.0	40.0	29.0	41.0
39	35.792164012408215	38.0	34.0	40.0	29.0	41.0
40	35.61416719625992	38.0	34.0	40.0	29.0	41.0
41	35.745609619510844	38.0	34.0	40.0	29.0	41.0
42	35.6660479339571	38.0	34.0	40.0	29.0	41.0
43	35.64612054591102	38.0	34.0	40.0	29.0	41.0
44	35.56877214066836	38.0	34.0	40.0	29.0	41.0
45	35.405143556475615	38.0	34.0	40.0	28.0	41.0
46	35.365302937826804	38.0	34.0	40.0	28.0	41.0
47	35.2645098063891	37.0	34.0	40.0	28.0	41.0
48	35.305221894328845	37.0	34.0	40.0	28.0	41.0
49	35.187394212437155	37.0	34.0	40.0	27.0	41.0
50	35.08549050822076	37.0	34.0	40.0	27.0	41.0
51	33.91146564145803	36.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	10.0
10	14.0
11	14.0
12	24.0
13	33.0
14	80.0
15	69.0
16	124.0
17	231.0
18	414.0
19	688.0
20	1174.0
21	1729.0
22	2409.0
23	2951.0
24	3519.0
25	4379.0
26	5169.0
27	6445.0
28	8184.0
29	10647.0
30	13919.0
31	18817.0
32	25405.0
33	36218.0
34	56146.0
35	62891.0
36	74068.0
37	101058.0
38	123584.0
39	75292.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.06896247962901	33.516331397434044	20.331032486613353	26.083673636323596
2	24.075676253877567	31.782988416065237	20.0431644717386	24.0981708583186
3	21.276435092841368	31.078576956715974	21.72931597525908	25.915671975183574
4	20.94404978386303	31.78141536680363	20.00965852246629	27.264876326867054
5	18.18602251348103	34.553757385466284	18.968929130984666	28.29129097006802
6	20.70510360102437	35.916175350947285	21.4076274012597	21.97109364676864
7	79.24125541915471	8.045360448507806	7.141171732933989	5.5722123994035
8	81.19057806414266	5.529110849635368	6.013138107432972	7.267172978789003
9	75.4896902351394	9.1496410301585	10.230325872885034	5.130342861817061
10	35.4963285030234	35.39565335028031	13.561886904050288	15.546131242645995
11	28.23434658679771	25.774569456417098	27.558721928935924	18.432362027849265
12	29.26375002359574	23.599671547314173	29.297098667941885	17.8394797611482
13	24.671075399397207	28.520012332706212	25.861244470731847	20.947667797164737
14	18.25051753320707	30.369289044655723	30.454233704782702	20.92595971735451
15	16.931201117494197	30.65967393834905	30.267040842651028	22.142084101505723
16	19.662486550428813	27.538272288534987	31.15911707891044	21.640124082125755
17	18.715668199865345	27.567688309727107	29.351368867467453	24.365274622940092
18	20.659327867511497	27.28705632145576	27.707375084158137	24.3462407268746
19	21.394256482536004	31.109566027169706	27.413057567310776	20.08311992298351
20	24.26318372586156	29.48806684830142	26.092168102336295	20.156581323500728
21	21.8411597777596	29.29646944823724	26.615993506452646	22.24637726755051
22	20.49274195070693	27.973535019222663	27.487462797384964	24.046260232685444
23	21.521830777652635	28.9398591806301	24.747525593511487	24.79078444820578
24	19.008727277303418	29.327301213764812	28.788689146589313	22.875282362342457
25	20.019411427888276	28.16308745524675	26.539071397559887	25.27842971930509
26	22.225927627149574	30.102657194812714	23.993248472569167	23.67816670546855
27	18.1251455070567	29.853643496699746	26.84738905283558	25.17382194340798
28	18.910097088600427	28.103626193157865	27.91863559999245	25.067641118249256
29	19.314685358686692	29.503325426139043	27.05440233566354	24.12758687951072
30	20.17294103582148	28.285156077947736	27.46544010772241	24.076462778508372
31	22.264624638985193	27.956231477344947	24.267588263794067	25.511555619875793
32	22.818652588924472	26.932491017888715	27.150358340621793	23.098498052565013
33	21.719720374763256	26.837006927708952	26.555903024659123	24.887369672868676
34	18.41427196134074	25.67924267116349	29.431437074883437	26.47504829261233
35	21.31639054408628	25.64023104947555	28.121244344887902	24.92213406155027
36	20.464269759071776	27.45773216634052	28.975882008720987	23.102116065866717
37	20.321436886117525	29.598494906466488	26.419676958603638	23.66039124881235
38	21.321109691871108	27.29523617761614	28.734418947063745	22.649235183449004
39	20.098693110673455	24.31194825297149	31.521547628785545	24.06781100756951
40	22.842248327848637	25.075978279335796	28.1495592315969	23.932214161218674
41	19.62347492874087	22.66150496768957	32.70904251637544	25.005977587194124
42	23.749425837019512	24.453679991442613	26.23389984080741	25.56299433073046
43	21.013106646447742	25.082427781308397	27.890320713283458	26.014144858960403
44	21.09695017209159	24.760739207309015	27.036784183933506	27.10552643666589
45	23.272477300899155	25.399239902596786	27.04228985634914	24.285992940154912
46	19.154706248780887	25.67011898544615	31.836786700812326	23.33838806496064
47	19.839611897286176	26.488576516262185	28.35610059964638	25.31571098680526
48	20.881127813398603	23.401467340351232	32.512883273452594	23.204521572797574
49	21.8148898550907	23.07568883827166	29.849868178471876	25.259553128165763
50	19.83756693324608	24.19805948643088	28.52064155241085	27.443732027912187
51	18.685780263894745	23.81392085674555	31.805011105727786	25.695287773631918
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3256.0
1	2583.5
2	1911.0
3	1294.0
4	677.0
5	1112.0
6	1547.0
7	1337.5
8	1128.0
9	1013.0
10	898.0
11	923.5
12	949.0
13	988.0
14	1027.0
15	1135.0
16	1243.0
17	1209.0
18	1175.0
19	1387.5
20	1600.0
21	1883.5
22	2167.0
23	2552.5
24	2938.0
25	3672.5
26	4965.5
27	5524.0
28	6508.5
29	7493.0
30	8944.5
31	10396.0
32	12135.5
33	13875.0
34	16258.5
35	18642.0
36	19724.5
37	20807.0
38	23956.0
39	27105.0
40	28616.0
41	30127.0
42	33555.5
43	36984.0
44	45385.0
45	53786.0
46	57395.5
47	61005.0
48	63935.5
49	66866.0
50	61560.5
51	56255.0
52	50075.5
53	43896.0
54	38745.5
55	33595.0
56	31033.5
57	28472.0
58	25878.5
59	23285.0
60	21159.5
61	19034.0
62	18007.5
63	16981.0
64	14309.5
65	11638.0
66	10113.5
67	8589.0
68	7222.5
69	5856.0
70	4901.0
71	3946.0
72	3471.0
73	2996.0
74	2284.5
75	1183.0
76	793.0
77	731.0
78	669.0
79	492.0
80	315.0
81	237.5
82	160.0
83	116.0
84	72.0
85	50.0
86	28.0
87	18.5
88	9.0
89	7.0
90	5.0
91	2.5
92	0.0
93	0.0
94	0.0
95	2.0
96	4.0
97	4.0
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	635708.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.26037663527925
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.38597498726465	24.965006993774573
2	9.346171835126082	6.03022046998413
3	3.3942767387975326	3.285019379939273
4	1.6202710389604362	2.0908221587239555
5	0.9464407496829219	1.5266267523873558
6	0.6321610106363289	1.2236251378360046
7	0.4770054237575566	1.077186223924283
8	0.3896393380056493	1.00559294367865
9	0.3413446285309689	0.9910715650954691
>10	4.721675010857389	36.57063086051488
>50	0.677263750643865	13.75174169152816
>100	0.0594263334502167	3.552209790009528
>500	0.005402393950019699	1.100994907706343
>1k	0.0024556336136453178	1.9445259033357243
>5k	4.911267227290636E-4	0.8847252215616951
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGCT	5584	0.8783907076834019	TruSeq Adapter, Index 20 (95% over 22bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCC	3098	0.4873306612469876	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGT	2803	0.4409257080294726	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2746	0.43195932723829183	No Hit
AATCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCT	2597	0.4085208932402927	No Hit
AAACTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCT	1029	0.16186676901973862	TruSeq Adapter, Index 27 (95% over 21bp)
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	956	0.1503835094099807	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	783	0.12316975718411599	No Hit
CCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGC	778	0.12238323255331063	TruSeq Adapter, Index 27 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.0775387442033135	0.0
2	0.0	0.0	0.0	1.4033172462828847	0.0
3	0.0	0.0	0.0	1.8662656439749068	0.0
4	0.0	0.0	0.0	3.982803425472072	0.0
5	0.0	0.0	0.0	4.269727610789859	0.0
6	0.0	0.0	0.0	5.670056063475684	0.0
7	0.0	0.0	0.0	6.692852693374946	0.0
8	0.0	0.0	0.0	7.298162049242734	0.0
9	0.0	0.0	0.0	8.733412195536316	0.0
10	0.0	0.0	0.0	10.116594411270583	0.0
11	0.0	0.0	0.0	12.150547106533189	0.0
12	0.0	0.0	0.0	12.926689612211897	0.0
13	0.0	0.0	0.0	13.24680513694967	0.0
14	0.0	0.0	0.0	13.67844985433564	0.0
15	0.0	0.0	0.0	13.986452899759009	0.0
16	0.0	0.0	0.0	14.608751187652192	0.0
17	1.5730492616106766E-4	0.0	0.0	15.368533981010149	0.0
18	1.5730492616106766E-4	0.0	0.0	16.334386227639104	0.0
19	1.5730492616106766E-4	0.0	0.0	16.802053773115958	0.0
20	1.5730492616106766E-4	0.0	0.0	17.24250756636695	0.0
21	1.5730492616106766E-4	0.0	0.0	17.77467013156984	0.0
22	1.5730492616106766E-4	0.0	0.0	18.361574811076785	0.0
23	1.5730492616106766E-4	0.0	0.0	18.927086020625822	0.0
24	1.5730492616106766E-4	0.0	0.0	19.357472298602502	0.0
25	1.5730492616106766E-4	0.0	0.0	19.723206251926985	0.0
26	1.5730492616106766E-4	0.0	0.0	20.02727667419633	0.0
27	1.5730492616106766E-4	0.0	0.0	20.35258326149742	0.0
28	1.5730492616106766E-4	0.0	0.0	20.70384516161508	0.0
29	1.5730492616106766E-4	0.0	0.0	21.079332020361548	0.0
30	1.5730492616106766E-4	0.0	0.0	21.52576340080666	0.0
31	1.5730492616106766E-4	0.0	0.0	21.878441045259773	0.0
32	1.5730492616106766E-4	0.0	0.0	22.240871595134873	0.0
33	1.5730492616106766E-4	0.0	0.0	22.596066118406565	0.0
34	1.5730492616106766E-4	0.0	0.0	22.945125749557974	0.0
35	1.5730492616106766E-4	0.0	0.0	23.336343100920548	0.0
36	1.5730492616106766E-4	0.0	0.0	23.701605139466547	0.0
37	1.5730492616106766E-4	0.0	0.0	24.04059725534365	0.0
38	1.5730492616106766E-4	0.0	0.0	24.40035362147401	0.0
39	1.5730492616106766E-4	0.0	0.0	24.76136842701366	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCGCG	35	1.2107739E-7	45.000004	1
TCACGCG	20	7.030905E-4	45.0	1
AACCCGC	25	3.888832E-5	45.0	45
GACACGA	25	3.888832E-5	45.0	25
CGCATCG	25	3.888832E-5	45.0	21
AAGCGAC	20	7.030905E-4	45.0	14
ATCGTAG	25	3.888832E-5	45.0	1
CTAACCG	25	3.888832E-5	45.0	1
TGACCGG	40	6.8066583E-9	45.0	2
TATCTCG	20	7.030905E-4	45.0	1
CGGGCTA	40	6.8066583E-9	45.0	6
GAGCCGT	20	7.030905E-4	45.0	23
CGTAACG	45	3.8380676E-10	45.0	1
TTACGAG	20	7.030905E-4	45.0	1
AACTCGA	20	7.030905E-4	45.0	11
ACTCCCG	25	3.888832E-5	45.0	1
TCGACGG	40	6.8066583E-9	45.0	2
GCATCGC	40	6.8066583E-9	45.0	22
AATCGGC	20	7.030905E-4	45.0	27
TATCCGG	50	2.1827873E-11	45.0	2
>>END_MODULE