##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552874_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	824245
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.23107935140644	31.0	31.0	34.0	28.0	34.0
2	31.518102020637066	31.0	31.0	34.0	30.0	34.0
3	31.693999963603055	31.0	31.0	34.0	30.0	34.0
4	35.608852950275704	37.0	35.0	37.0	35.0	37.0
5	34.961965192388185	37.0	35.0	37.0	32.0	37.0
6	35.06647538049973	36.0	35.0	37.0	32.0	37.0
7	35.19340123385644	37.0	35.0	37.0	33.0	37.0
8	35.53071113564535	37.0	35.0	37.0	33.0	37.0
9	37.13081790001759	39.0	37.0	39.0	34.0	39.0
10	36.35793605056749	38.0	35.0	39.0	32.0	39.0
11	36.56390272309811	38.0	35.0	39.0	32.0	39.0
12	36.390285655357324	38.0	35.0	39.0	32.0	39.0
13	36.2318946429763	38.0	35.0	39.0	32.0	39.0
14	37.286549508944546	39.0	36.0	40.0	32.0	41.0
15	37.202192309325504	39.0	36.0	40.0	32.0	41.0
16	37.08362319455987	39.0	35.0	40.0	32.0	41.0
17	37.043580488810974	38.0	36.0	40.0	32.0	41.0
18	36.95009614859659	38.0	36.0	40.0	32.0	41.0
19	37.12402137713908	39.0	35.0	40.0	32.0	41.0
20	36.958478365049224	39.0	35.0	40.0	31.0	41.0
21	37.09894266874534	39.0	35.0	40.0	32.0	41.0
22	37.09311187814303	39.0	35.0	40.0	32.0	41.0
23	37.007028250095544	39.0	35.0	40.0	32.0	41.0
24	36.56865373766295	38.0	35.0	40.0	31.0	41.0
25	36.79680798791622	39.0	35.0	40.0	31.0	41.0
26	36.67965471431431	39.0	35.0	40.0	31.0	41.0
27	36.570398364563935	39.0	35.0	40.0	31.0	41.0
28	36.609059199631176	39.0	35.0	40.0	31.0	41.0
29	36.36251478625894	38.0	35.0	40.0	30.0	41.0
30	36.405195057294854	38.0	35.0	40.0	30.0	41.0
31	36.346583843396076	38.0	35.0	40.0	30.0	41.0
32	36.32839507670656	38.0	35.0	40.0	30.0	41.0
33	36.19341943232898	38.0	35.0	40.0	30.0	41.0
34	36.216903954528085	38.0	35.0	40.0	30.0	41.0
35	36.12683122129949	38.0	35.0	40.0	30.0	41.0
36	36.03997355155324	38.0	35.0	40.0	30.0	41.0
37	35.993505571765674	38.0	35.0	40.0	30.0	41.0
38	35.86400402792859	38.0	35.0	40.0	30.0	41.0
39	35.84590746683328	38.0	35.0	40.0	30.0	41.0
40	35.61702406444686	38.0	34.0	40.0	29.0	41.0
41	35.70652779210065	38.0	34.0	40.0	29.0	41.0
42	35.68844457655187	38.0	34.0	40.0	29.0	41.0
43	35.664541489484314	38.0	34.0	40.0	29.0	41.0
44	35.58406663067413	38.0	34.0	40.0	29.0	41.0
45	35.41788424558232	38.0	34.0	40.0	28.0	41.0
46	35.36423757499287	38.0	34.0	40.0	28.0	41.0
47	35.2594083069961	38.0	34.0	40.0	28.0	41.0
48	35.289088802479846	38.0	34.0	40.0	28.0	41.0
49	35.15372249755837	37.0	34.0	40.0	27.0	41.0
50	35.04507033709638	37.0	34.0	40.0	27.0	41.0
51	33.77577540658421	36.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	3.0
10	4.0
11	18.0
12	26.0
13	20.0
14	40.0
15	76.0
16	135.0
17	245.0
18	470.0
19	820.0
20	1452.0
21	2150.0
22	3040.0
23	3742.0
24	4628.0
25	5284.0
26	6063.0
27	7571.0
28	9706.0
29	12300.0
30	16412.0
31	22400.0
32	31442.0
33	44915.0
34	70011.0
35	81441.0
36	98803.0
37	137445.0
38	165923.0
39	97655.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.136731190362088	33.33207966078047	20.96548962990373	25.56569951895371
2	23.52286031459093	32.36816723183034	20.17446269009821	23.93450976348052
3	20.671402313632477	30.55499275094177	21.917633713277	26.855971222148757
4	20.584898907485034	32.106109227232196	19.73369568514216	27.57529618014061
5	17.467985853720677	34.98171053509576	19.550497728224013	27.999805882959556
6	20.683291982359613	35.42102166224848	21.743656315779898	22.152030039612008
7	80.89196780083591	6.798464048917493	7.169227596163761	5.140340554082827
8	82.96404588441544	5.131726610413166	5.713349792840721	6.190877712330678
9	76.59227535502187	8.83281063276089	9.817469320408375	4.7574446918088675
10	33.30854296962675	37.516515113831446	13.633810335519172	15.541131581022633
11	25.062572414755323	26.732221608866297	29.826568556679145	18.37863741969924
12	27.16316143864992	24.65383472147238	30.030148802843815	18.15285503703389
13	23.984009608793503	29.522290095784626	26.664887260462606	19.828813034959268
14	18.453251157119546	31.055086776383234	30.100273583703874	20.391388482793342
15	16.69467209385559	31.728308937269865	30.09566330399335	21.481355664881193
16	18.37220729273456	28.696564734999907	30.784414828115423	22.1468131441501
17	17.94818288251673	28.603631201887787	29.25076888546488	24.197417030130605
18	20.444891992065468	28.24318012241506	28.142117938234385	23.169809947285092
19	19.87794891082142	32.49907491097914	27.600531395398214	20.02244478280123
20	23.234717832683245	31.174954048856833	25.9694629630753	19.620865155384625
21	20.685718445365154	30.37931682934079	27.349756443775817	21.585208281518238
22	20.665214832968353	28.095165879077218	27.533318370144798	23.70630091780963
23	20.260116834193717	30.232637140655992	25.223932204623623	24.283313820526665
24	17.62073169991932	31.512111083476395	29.0703613610031	21.796795855601186
25	18.88673877305898	29.698451309986716	27.642145235943193	23.772664681011108
26	21.260668854527477	31.729764815073185	25.33203113152036	21.677535198878974
27	17.803929656837468	30.20291296883815	28.00684262567562	23.986314748648763
28	18.583673543667235	28.718160255749204	31.97119788412426	20.726968316459306
29	18.89413948522587	29.145945683625623	29.25198211696765	22.707932714180856
30	21.889608065563028	28.430381743292347	27.992526493942943	21.68748369720168
31	23.089736668102322	28.98895352716729	26.792519214553923	21.128790590176465
32	22.997531073891864	27.853490163725592	25.982687186455482	23.166291575927062
33	22.401713082881912	28.329683528562505	27.661435616837228	21.60716777171836
34	20.448046393972668	26.630674132084515	28.825409920593998	24.095869553348823
35	21.58678548247184	26.87962923645275	29.745767338594714	21.787817942480693
36	23.82180055687326	26.955698851676384	30.212133528259194	19.010367063191165
37	22.385819750195633	28.919192715758058	26.20919750802249	22.485790026023817
38	21.811839926235525	28.660167789916834	27.92228038993261	21.605711893915036
39	21.010257872355915	26.770074431752693	27.789188894078826	24.43047880181257
40	24.516496915358903	28.015335246195	24.95471613415914	22.513451704286954
41	23.09471091726368	24.54779828813035	30.28953769813587	22.067953096470102
42	25.423023494228055	26.31753908121978	25.25135123658621	23.008086187965958
43	21.031974716255483	26.527792100649684	28.444576551874746	23.995656631220086
44	22.546330278012	25.215682230404795	27.137683577091764	25.100303914491445
45	23.893381215536643	27.31226759034025	27.053606633949855	21.74074456017325
46	20.373675302852913	26.637710874800575	30.48777972568836	22.500834096658153
47	21.509138666294607	27.991191939289894	27.217878179424808	23.281791214990687
48	22.778421464491746	24.488349944494658	31.80401458304266	20.92921400797093
49	22.47511358879945	24.01992126127547	29.970215166607016	23.53474998331807
50	20.8468355889329	24.75805130756025	27.702806811081654	26.69230629242519
51	20.13394075790572	24.832301075529728	30.894454925416593	24.13930324114796
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4473.0
1	3547.0
2	2621.0
3	1869.0
4	1117.0
5	1884.5
6	2652.0
7	2335.0
8	2018.0
9	1912.5
10	1807.0
11	1885.5
12	1964.0
13	2063.0
14	2162.0
15	2205.5
16	2249.0
17	2451.5
18	2654.0
19	2837.5
20	3021.0
21	3170.5
22	3320.0
23	4003.5
24	4687.0
25	5333.5
26	6779.5
27	7579.0
28	8979.5
29	10380.0
30	12162.5
31	13945.0
32	15787.0
33	17629.0
34	20237.0
35	22845.0
36	25934.5
37	29024.0
38	31237.0
39	33450.0
40	37485.5
41	41521.0
42	47432.5
43	53344.0
44	60646.0
45	67948.0
46	74893.0
47	81838.0
48	87108.5
49	92379.0
50	86370.0
51	80361.0
52	70819.5
53	61278.0
54	52515.0
55	43752.0
56	37952.5
57	32153.0
58	27970.0
59	23787.0
60	22349.0
61	20911.0
62	18388.0
63	15865.0
64	13485.5
65	11106.0
66	9087.5
67	7069.0
68	6065.5
69	5062.0
70	4322.0
71	3582.0
72	2861.0
73	2140.0
74	2013.0
75	1558.5
76	1231.0
77	968.5
78	706.0
79	582.5
80	459.0
81	306.5
82	154.0
83	106.5
84	59.0
85	47.5
86	36.0
87	24.5
88	13.0
89	12.5
90	12.0
91	8.5
92	5.0
93	5.0
94	5.0
95	4.0
96	3.0
97	2.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	824245.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.314848735703805
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.18593557801606	26.486237038834005
2	9.931352133654038	6.815856924146951
3	3.5742048742919468	3.679448988352302
4	1.7036288457280366	2.3383906457175705
5	1.00066135745504	1.7168771558366867
6	0.6689513123027607	1.3772977855931878
7	0.5437275449447471	1.306054992075021
8	0.41772729063106245	1.1467399032624233
9	0.3369630528060469	1.0406552567904426
>10	3.8697153910661712	30.360810669777216
>50	0.6887240385532822	15.576147607791249
>100	0.07063836085663713	4.29001847018824
>500	0.004591493455681391	1.0873815779452716
>1k	0.002825534434265472	2.0218122473565563
>5k	3.53191804283184E-4	0.7562707363328685
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGCT	6240	0.7570564577279814	Illumina Single End Adapter 1 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3814	0.4627264951561732	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCC	2493	0.30245861364036175	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGT	2408	0.2921461458668236	No Hit
AATCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCT	2298	0.27880059933636236	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1735	0.21049566573045636	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1411	0.17118696504073425	No Hit
CCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC	1265	0.1534737851003039	Illumina Single End Adapter 1 (95% over 21bp)
AAACTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCT	1258	0.1526245230483655	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	842	0.10215409253316672	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.6396945083076027E-4	0.0	0.0	0.9066479020194238	0.0
2	4.8529260110768036E-4	0.0	0.0	1.272194553803784	0.0
3	4.8529260110768036E-4	0.0	0.0	1.7304320923997112	0.0
4	4.8529260110768036E-4	0.0	0.0	3.923226710504765	0.0
5	4.8529260110768036E-4	0.0	0.0	4.227262525098727	0.0
6	4.8529260110768036E-4	0.0	0.0	5.855297878664717	0.0
7	4.8529260110768036E-4	0.0	0.0	7.075808770450534	0.0
8	4.8529260110768036E-4	0.0	0.0	7.743571389574702	0.0
9	4.8529260110768036E-4	0.0	0.0	9.182463951858974	0.0
10	4.8529260110768036E-4	0.0	0.0	10.798366990397273	0.0
11	4.8529260110768036E-4	0.0	0.0	12.991646901103435	0.0
12	4.8529260110768036E-4	0.0	0.0	13.953618159649134	0.0
13	4.8529260110768036E-4	0.0	0.0	14.31928613458377	0.0
14	4.8529260110768036E-4	0.0	0.0	14.763571510897853	0.0
15	4.8529260110768036E-4	0.0	0.0	15.117592463405904	0.0
16	4.8529260110768036E-4	0.0	0.0	15.811682206140164	0.0
17	4.8529260110768036E-4	0.0	0.0	16.75060206613325	0.0
18	4.8529260110768036E-4	0.0	0.0	17.767411388604117	0.0
19	4.8529260110768036E-4	0.0	0.0	18.32392067892435	0.0
20	4.8529260110768036E-4	0.0	0.0	18.816371345898368	0.0
21	4.8529260110768036E-4	0.0	0.0	19.435240735460937	0.0
22	4.8529260110768036E-4	0.0	0.0	20.15444437030252	0.0
23	6.066157513846005E-4	0.0	0.0	20.823541544079735	0.0
24	6.066157513846005E-4	0.0	0.0	21.320481167614	0.0
25	6.066157513846005E-4	0.0	0.0	21.730553415549988	0.0
26	6.066157513846005E-4	0.0	0.0	22.134923475422962	0.0
27	6.066157513846005E-4	0.0	0.0	22.529102390672676	0.0
28	6.066157513846005E-4	0.0	0.0	22.931652603291496	0.0
29	6.066157513846005E-4	0.0	0.0	23.37314754714921	0.0
30	6.066157513846005E-4	0.0	0.0	23.902359128657135	0.0
31	6.066157513846005E-4	0.0	0.0	24.32965926393245	0.0
32	6.066157513846005E-4	0.0	0.0	24.741066066521483	0.0
33	7.279389016615205E-4	0.0	0.0	25.153443454312736	0.0
34	7.279389016615205E-4	0.0	0.0	25.5447106139558	0.0
35	7.279389016615205E-4	0.0	0.0	26.00998489526779	0.0
36	7.279389016615205E-4	0.0	0.0	26.435343860138673	0.0
37	7.279389016615205E-4	0.0	0.0	26.846993309028264	0.0
38	7.279389016615205E-4	0.0	0.0	27.265558177483637	0.0
39	7.279389016615205E-4	0.0	0.0	27.66871500585384	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATCG	20	7.0321246E-4	45.0	1
AAATCCG	20	7.0321246E-4	45.0	1
CGTATAG	20	7.0321246E-4	45.0	1
CGACGTG	25	3.889842E-5	45.0	13
AGATACG	25	3.889842E-5	45.0	1
ATTACCG	45	3.8380676E-10	45.0	1
CACGCTA	20	7.0321246E-4	45.0	42
TCCTCGC	25	3.889842E-5	45.0	11
ATTAACG	40	6.8102963E-9	45.0	1
TCGGCCA	20	7.0321246E-4	45.0	14
ATCGTAG	25	3.889842E-5	45.0	1
CGCTATC	20	7.0321246E-4	45.0	18
CTACGCG	20	7.0321246E-4	45.0	1
GTCGTCG	20	7.0321246E-4	45.0	25
GCTAGCG	40	6.8102963E-9	45.0	1
TACTCGA	20	7.0321246E-4	45.0	32
TCATCGA	25	3.889842E-5	45.0	16
CGTCGAA	20	7.0321246E-4	45.0	31
CCGAACT	20	7.0321246E-4	45.0	11
TAAGCGA	45	3.8380676E-10	45.0	13
>>END_MODULE