FastQCFastQC Report
Sat 18 Jun 2016
SRR3552873_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552873_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences494329
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGCT55241.1174743945833645No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50021.011876705594857No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCC22420.4535440971498739No Hit
AATCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCT20000.40458884669926304No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGT19870.40195901919571786No Hit
AAACTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCT16960.3430913420009751No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12560.2540817957271372No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10560.21362291105721087No Hit
AAAAACTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTT10160.20553113412322563No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG8360.16911813792029196No Hit
CCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGC7570.15313687847567106No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6260.12663630901686934No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA5700.11530782130928997No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCT5480.11085734399559807No Hit
AATGACTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTT5270.10660916110525581No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5080.10276556706161281No Hit
AACTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTG5040.10195638936821429No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACA351.2100463E-745.00000431
GGTACGA650.045.0000049
GTAGCAC253.8875678E-545.00000435
ATATACG253.8875678E-545.0000041
CGTAGCG253.8875678E-545.0000041
CGTAGCA253.8875678E-545.00000434
CACTACG253.8875678E-545.0000041
CATCGCC253.8875678E-545.00000423
GGCATAT253.8875678E-545.0000048
TCATACG253.8875678E-545.0000041
GTAAGCG253.8875678E-545.0000041
CAATACG502.1827873E-1145.0000041
GCACTAG253.8875678E-545.0000041
TTCTACG406.8012014E-945.01
AATCCCG207.029382E-445.01
CTCACGA207.029382E-445.024
TGCCAAC207.029382E-445.012
CGACGGT207.029382E-445.028
CGACGAT207.029382E-445.022
CCGATCA207.029382E-445.017