##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552873_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	494329
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.10403799898448	31.0	31.0	33.0	28.0	34.0
2	31.38778627189584	31.0	31.0	34.0	30.0	34.0
3	31.583427231661506	31.0	31.0	34.0	30.0	34.0
4	35.52944091890219	37.0	35.0	37.0	33.0	37.0
5	34.90132280323429	35.0	35.0	37.0	32.0	37.0
6	35.006076924477426	35.0	35.0	37.0	32.0	37.0
7	34.94826927006103	36.0	35.0	37.0	32.0	37.0
8	35.402375745707815	37.0	35.0	37.0	33.0	37.0
9	36.859508545927916	39.0	37.0	39.0	33.0	39.0
10	36.325961050231726	38.0	35.0	39.0	32.0	39.0
11	36.51287907446255	38.0	35.0	39.0	32.0	39.0
12	36.15574243064842	37.0	35.0	39.0	32.0	39.0
13	35.74876853269786	37.0	35.0	39.0	30.0	39.0
14	36.79866445221705	39.0	35.0	40.0	31.0	41.0
15	36.830157648044114	38.0	35.0	40.0	32.0	41.0
16	36.77117466302806	38.0	35.0	40.0	32.0	41.0
17	36.687643249738535	38.0	35.0	40.0	32.0	41.0
18	36.62236890815631	38.0	35.0	40.0	31.0	41.0
19	36.68254947615859	38.0	35.0	40.0	31.0	41.0
20	36.57859037199921	38.0	35.0	40.0	31.0	41.0
21	36.646061631019016	38.0	35.0	40.0	31.0	41.0
22	36.65324308304793	38.0	35.0	40.0	31.0	41.0
23	36.556250998828716	38.0	35.0	40.0	31.0	41.0
24	36.07677477954965	38.0	34.0	40.0	30.0	41.0
25	36.34608732241078	38.0	35.0	40.0	31.0	41.0
26	36.27222558255737	38.0	35.0	40.0	31.0	41.0
27	36.15067090945504	38.0	35.0	40.0	30.0	41.0
28	36.22256230162503	38.0	35.0	40.0	30.0	41.0
29	35.74606587920191	38.0	34.0	40.0	29.0	41.0
30	35.80745414491159	38.0	34.0	40.0	30.0	41.0
31	35.717659696275156	38.0	34.0	40.0	29.0	41.0
32	35.66561945586846	38.0	34.0	40.0	29.0	41.0
33	35.62108433856804	38.0	34.0	40.0	29.0	41.0
34	35.55226579868873	38.0	34.0	40.0	29.0	41.0
35	35.52353189879614	38.0	34.0	40.0	29.0	41.0
36	35.380287217622275	38.0	34.0	40.0	28.0	41.0
37	35.241225985123265	38.0	34.0	40.0	27.0	41.0
38	35.10814862166695	37.0	34.0	40.0	27.0	41.0
39	35.08616528668154	37.0	34.0	40.0	27.0	41.0
40	34.89891752254066	37.0	34.0	40.0	26.0	41.0
41	35.00189752169102	37.0	34.0	40.0	27.0	41.0
42	34.95881892423871	37.0	34.0	40.0	27.0	41.0
43	34.929575242399295	37.0	34.0	40.0	27.0	41.0
44	34.84601955377896	37.0	34.0	40.0	26.0	41.0
45	34.6417365762478	37.0	33.0	40.0	26.0	41.0
46	34.58023300271682	37.0	33.0	40.0	26.0	41.0
47	34.46729202616071	36.0	33.0	40.0	25.0	41.0
48	34.49471505818999	36.0	33.0	40.0	26.0	41.0
49	34.38347335479003	36.0	33.0	40.0	26.0	41.0
50	34.27209611412642	36.0	33.0	39.0	25.0	41.0
51	33.10462667575643	35.0	31.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	2.0
9	4.0
10	3.0
11	16.0
12	14.0
13	11.0
14	39.0
15	67.0
16	114.0
17	224.0
18	426.0
19	799.0
20	1308.0
21	2063.0
22	2749.0
23	3372.0
24	3739.0
25	4169.0
26	4769.0
27	5559.0
28	6719.0
29	8775.0
30	11305.0
31	15357.0
32	21209.0
33	30393.0
34	49401.0
35	52925.0
36	57277.0
37	76669.0
38	86983.0
39	47864.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.849187484448613	35.17616809857403	20.194243105300316	25.780401311677043
2	23.380582567480364	34.548448502920124	19.7091410781079	22.36182785149162
3	19.78601295898076	33.19246898320754	20.841180671172438	26.180337386639263
4	21.326282698364853	33.049446825899345	19.256203864228073	26.368066611507718
5	17.235282574965254	36.083660881720476	18.207711868006935	28.473344675307338
6	19.64946422321976	37.57457078180726	20.71333059561547	22.062634399357513
7	75.65710286064544	10.2933471432993	7.810587685529273	6.238962310525985
8	78.16312617710068	7.835267605177928	6.482929385085641	7.518676832635755
9	72.66354998391759	10.809804806110911	10.884451448326923	5.642193761644573
10	37.18758154993942	32.6375753799595	13.299442274274826	16.875400795826263
11	31.25347693540132	28.164845679699148	22.524472567864724	18.05720481703481
12	30.07996698555011	25.841696521951977	26.410953029257843	17.667383463240068
13	23.93062110456801	34.13293575735998	22.44294791525482	19.49349522281719
14	17.672845412670508	34.420800721786506	27.436181166793776	20.470172698749213
15	15.407350165577984	31.735342251820143	30.009163937377735	22.84814364522413
16	17.247622534789585	28.851432952547796	30.602898069908907	23.298046442753712
17	16.561642145210982	29.422510109663808	26.987694430227645	27.02815331489757
18	21.07523531898796	28.166666329509294	25.690380293286452	25.067718058216286
19	21.591086098529523	32.47149165838945	25.10615399865272	20.831268244428305
20	25.19455666165651	30.144498906598642	23.471210469140996	21.189733962603853
21	20.66498222843491	31.81969902635694	24.90750087492338	22.60781787028477
22	20.203750943197747	29.08144171189633	24.73615749834624	25.97864984655968
23	20.700788341367794	31.270065078115994	22.159128839295285	25.87001774122093
24	17.557739885784567	31.86258544410706	26.982434775220554	23.597239894887817
25	18.33293211606036	29.82730125078642	26.838198851372262	25.00156778178096
26	21.676656639606414	32.59934173394642	23.357521003218505	22.36648062322866
27	17.94088552360877	31.71915869795217	25.781817372640486	24.55813840579857
28	20.177452668162296	29.083464656129827	30.057107715711602	20.68197495999628
29	17.606290547388483	30.78233322342003	28.31130684220428	23.30006938698721
30	20.145490149273055	30.444703830849495	26.485195082627154	22.924610937250293
31	26.305153045845987	29.92662781265109	22.40977162982548	21.358447511677443
32	23.34760857647437	27.972059094246948	23.785373708602975	24.894958620675702
33	22.330269921449077	27.766123371277025	24.00911134082767	25.894495366446236
34	18.9396130916859	29.599922318941434	25.955992871144524	25.504471718228146
35	21.913745703772182	27.337259193775804	27.63099069647947	23.11800440597254
36	23.61119821009894	29.019337323927992	26.957754855571896	20.41170961040117
37	21.64752624264407	31.62205737474435	22.386710065563623	24.343706317047957
38	23.50701658207388	29.55116936291417	24.18955796645554	22.752256088556408
39	22.371335689389053	25.69240323751995	25.75875580837863	26.17750526471237
40	24.17721800663121	26.44089260391359	22.923801759556895	26.45808762989831
41	20.612183383940653	23.990904842726202	28.596137390280564	26.800774383052584
42	26.35613124053009	25.558079740415796	23.84302761925762	24.242761399796493
43	22.78846679033599	25.107772354039515	27.557760115226905	24.546000740397588
44	23.03000633181545	24.94654369862986	24.988014055416535	27.035435914138155
45	25.511552023045382	27.12019727752165	24.541145674237196	22.82710502519577
46	20.62027516087464	25.335353580307853	29.367283732089355	24.67708752672815
47	20.94880130439444	27.435371989100375	25.464417422404917	26.151409284100264
48	23.132569604453714	24.07890291688329	30.914229187443993	21.874298291219006
49	23.356914119948456	23.61888539818623	28.149269009101225	24.87493147276409
50	20.37246449227134	24.803521541321672	26.6537063372774	28.17030762912959
51	20.362552065527208	24.333389301457125	30.078146335739962	25.2259122972757
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5746.0
1	4664.5
2	3583.0
3	2430.0
4	1277.0
5	1462.0
6	1647.0
7	1515.0
8	1383.0
9	1266.5
10	1150.0
11	1198.5
12	1247.0
13	1285.5
14	1324.0
15	1366.0
16	1408.0
17	1545.5
18	1683.0
19	1669.0
20	1655.0
21	1691.5
22	1728.0
23	2099.5
24	2471.0
25	2621.5
26	3389.5
27	4007.0
28	4339.0
29	4671.0
30	5113.0
31	5555.0
32	6340.0
33	7125.0
34	8768.0
35	10411.0
36	10189.5
37	9968.0
38	10883.5
39	11799.0
40	15208.0
41	18617.0
42	23882.5
43	29148.0
44	36867.5
45	44587.0
46	46335.0
47	48083.0
48	51204.5
49	54326.0
50	50794.0
51	47262.0
52	41889.5
53	36517.0
54	32810.5
55	29104.0
56	26357.5
57	23611.0
58	21281.5
59	18952.0
60	17435.5
61	15919.0
62	14397.0
63	12875.0
64	10957.0
65	9039.0
66	8382.0
67	7725.0
68	6379.0
69	5033.0
70	4131.0
71	3229.0
72	3204.0
73	3179.0
74	2674.0
75	1589.0
76	1009.0
77	833.0
78	657.0
79	539.5
80	422.0
81	282.5
82	143.0
83	97.5
84	52.0
85	44.5
86	37.0
87	25.0
88	13.0
89	9.0
90	5.0
91	4.5
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	494329.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.15739137578351
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.56525753507346	24.47888477559508
2	9.475533746645434	5.904658268773522
3	3.411641929225931	3.188935884687763
4	1.5667604663503656	1.9526467616873373
5	0.8738038973205007	1.3612725007249895
6	0.5839076677743711	1.091582383930023
7	0.3904709014659313	0.8516238288480313
8	0.3207288830756899	0.7994460268405744
9	0.26049355653008077	0.7304669722509762
>10	3.342103918519573	25.48760699220449
>50	1.0748585964580348	22.71246241250448
>100	0.12334442910174172	6.02043534449527
>500	0.005220928215946739	0.9914767204728092
>1k	0.004568312188953397	2.288163973642437
>5k	0.0013052320539866848	2.1403371533422453
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGCT	5524	1.1174743945833645	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5002	1.011876705594857	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCC	2242	0.4535440971498739	No Hit
AATCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCT	2000	0.40458884669926304	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGT	1987	0.40195901919571786	No Hit
AAACTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCT	1696	0.3430913420009751	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1256	0.2540817957271372	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1056	0.21362291105721087	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTT	1016	0.20553113412322563	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	836	0.16911813792029196	No Hit
CCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGC	757	0.15313687847567106	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	626	0.12663630901686934	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	570	0.11530782130928997	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCT	548	0.11085734399559807	No Hit
AATGACTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTT	527	0.10660916110525581	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	508	0.10276556706161281	No Hit
AACTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTG	504	0.10195638936821429	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.0458884669926305E-4	0.0	0.0	1.3250284729400865	0.0
2	4.0458884669926305E-4	0.0	0.0	1.7221324259754132	0.0
3	4.0458884669926305E-4	0.0	0.0	2.247491043414406	0.0
4	4.0458884669926305E-4	0.0	0.0	4.714269241739813	0.0
5	4.0458884669926305E-4	0.0	0.0	5.100651590337609	0.0
6	4.0458884669926305E-4	0.0	0.0	6.846857052691629	0.0
7	4.0458884669926305E-4	0.0	0.0	8.233787619176702	0.0
8	4.0458884669926305E-4	0.0	0.0	9.045999728925473	0.0
9	4.0458884669926305E-4	0.0	0.0	10.547631233449788	0.0
10	4.0458884669926305E-4	0.0	0.0	12.248320450550139	0.0
11	4.0458884669926305E-4	0.0	0.0	14.530606134780683	0.0
12	4.0458884669926305E-4	0.0	0.0	15.49211152896148	0.0
13	4.0458884669926305E-4	0.0	0.0	15.89953249758764	0.0
14	4.0458884669926305E-4	0.0	0.0	16.358336249744603	0.0
15	4.0458884669926305E-4	0.0	0.0	16.70082070847553	0.0
16	4.0458884669926305E-4	0.0	0.0	17.38012538208359	0.0
17	4.0458884669926305E-4	0.0	0.0	18.17736770450449	0.0
18	4.0458884669926305E-4	0.0	0.0	19.316285307962914	0.0
19	4.0458884669926305E-4	0.0	0.0	19.788035903214254	0.0
20	4.0458884669926305E-4	0.0	0.0	20.222564324569266	0.0
21	4.0458884669926305E-4	0.0	0.0	20.72506367216975	0.0
22	4.0458884669926305E-4	0.0	0.0	21.192768378954096	0.0
23	4.0458884669926305E-4	0.0	0.0	21.72864630640727	0.0
24	4.0458884669926305E-4	0.0	0.0	22.068096348787954	0.0
25	4.0458884669926305E-4	0.0	0.0	22.384687121330128	0.0
26	4.0458884669926305E-4	0.0	0.0	22.667090136326213	0.0
27	4.0458884669926305E-4	0.0	0.0	22.972150126737457	0.0
28	4.0458884669926305E-4	0.0	0.0	23.270332106754815	0.0
29	4.0458884669926305E-4	0.0	0.0	23.58530452391019	0.0
30	4.0458884669926305E-4	0.0	0.0	24.060696418781824	0.0
31	4.0458884669926305E-4	0.0	0.0	24.34229025608451	0.0
32	4.0458884669926305E-4	0.0	0.0	24.635212581094777	0.0
33	4.0458884669926305E-4	0.0	0.0	24.967986907504923	0.0
34	4.0458884669926305E-4	0.0	0.0	25.263741354442082	0.0
35	4.0458884669926305E-4	0.0	0.0	25.607034990866406	0.0
36	4.0458884669926305E-4	0.0	0.0	25.866376441600636	0.0
37	4.0458884669926305E-4	0.0	0.0	26.14331750716628	0.0
38	4.0458884669926305E-4	0.0	0.0	26.41803333407508	0.0
39	6.068832700488946E-4	0.0	0.0	26.712371720048793	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATACA	35	1.2100463E-7	45.000004	31
GGTACGA	65	0.0	45.000004	9
GTAGCAC	25	3.8875678E-5	45.000004	35
ATATACG	25	3.8875678E-5	45.000004	1
CGTAGCG	25	3.8875678E-5	45.000004	1
CGTAGCA	25	3.8875678E-5	45.000004	34
CACTACG	25	3.8875678E-5	45.000004	1
CATCGCC	25	3.8875678E-5	45.000004	23
GGCATAT	25	3.8875678E-5	45.000004	8
TCATACG	25	3.8875678E-5	45.000004	1
GTAAGCG	25	3.8875678E-5	45.000004	1
CAATACG	50	2.1827873E-11	45.000004	1
GCACTAG	25	3.8875678E-5	45.000004	1
TTCTACG	40	6.8012014E-9	45.0	1
AATCCCG	20	7.029382E-4	45.0	1
CTCACGA	20	7.029382E-4	45.0	24
TGCCAAC	20	7.029382E-4	45.0	12
CGACGGT	20	7.029382E-4	45.0	28
CGACGAT	20	7.029382E-4	45.0	22
CCGATCA	20	7.029382E-4	45.0	17
>>END_MODULE