FastQCFastQC Report
Sat 18 Jun 2016
SRR3552871_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552871_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences561822
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGCT43530.7748005596078473Illumina Single End Adapter 1 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27470.48894489713823946No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCC21990.3914051069555838No Hit
AATCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCT19790.35224679702824024No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGT19730.3511788431211309No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG9300.16553285560195222No Hit
AAACTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCT9060.16126103997351474No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7750.13794404633496019No Hit
CCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGC6950.12370466090683525Illumina Single End Adapter 1 (95% over 21bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6670.11872087600699154No Hit
AAAAACTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTT5900.1050154675324213No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCCG207.030205E-445.01
CTATACG253.8882514E-545.01
GCCGGAT207.030205E-445.020
CGATTGA207.030205E-445.034
ATGTCGC207.030205E-445.035
CGTTCGA207.030205E-445.014
CTAACGG750.045.02
ACTAGCG351.2104465E-745.01
ATAACGG850.045.02
ATACGAG253.8882514E-545.01
ATTGCGG351.2104465E-745.02
TATCGGG253.8882514E-545.03
CGATATG351.2104465E-745.01
TCAACGT207.030205E-445.015
ATCGAAT207.030205E-445.032
TCTATCG207.030205E-445.037
ACGCATG302.1635133E-644.9999961
TAGCACG302.1635133E-644.9999961
TATTAGG302.1635133E-644.9999962
TAACGGG2400.043.1249963