##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552871_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	561822
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.17807597424095	31.0	31.0	33.0	28.0	34.0
2	31.458780539031935	31.0	31.0	34.0	30.0	34.0
3	31.622592208920263	31.0	31.0	34.0	30.0	34.0
4	35.562930963899596	37.0	35.0	37.0	33.0	37.0
5	34.950779784344505	35.0	35.0	37.0	32.0	37.0
6	35.05490351036449	36.0	35.0	37.0	32.0	37.0
7	35.12267586530965	37.0	35.0	37.0	32.0	37.0
8	35.51951685765243	37.0	35.0	37.0	33.0	37.0
9	36.99741021177526	39.0	37.0	39.0	34.0	39.0
10	36.34228100715173	38.0	35.0	39.0	32.0	39.0
11	36.588389205121906	38.0	35.0	39.0	32.0	39.0
12	36.38832405993357	38.0	35.0	39.0	32.0	39.0
13	36.151256091787076	38.0	35.0	39.0	32.0	39.0
14	37.238265856445636	39.0	36.0	40.0	32.0	41.0
15	37.18149698659006	39.0	36.0	40.0	32.0	41.0
16	37.05867338765659	39.0	35.0	40.0	32.0	41.0
17	37.013495377539506	38.0	35.0	40.0	32.0	41.0
18	36.95031344447174	38.0	36.0	40.0	32.0	41.0
19	37.06551897220116	39.0	35.0	40.0	32.0	41.0
20	36.95789591721221	39.0	35.0	40.0	31.0	41.0
21	37.07642456151593	39.0	35.0	40.0	32.0	41.0
22	37.071652587474325	39.0	35.0	40.0	32.0	41.0
23	36.990966889869036	39.0	35.0	40.0	32.0	41.0
24	36.56871215438342	38.0	35.0	40.0	31.0	41.0
25	36.78723332300978	39.0	35.0	40.0	31.0	41.0
26	36.676272912061116	39.0	35.0	40.0	31.0	41.0
27	36.626513379682535	39.0	35.0	40.0	31.0	41.0
28	36.64605871610581	39.0	35.0	40.0	31.0	41.0
29	36.17785882361317	38.0	35.0	40.0	30.0	41.0
30	36.30702250890852	38.0	35.0	40.0	30.0	41.0
31	36.22758275752818	38.0	35.0	40.0	30.0	41.0
32	36.17115207307653	38.0	35.0	40.0	30.0	41.0
33	36.13152920319959	38.0	35.0	40.0	30.0	41.0
34	36.126018561038904	38.0	35.0	40.0	30.0	41.0
35	35.98613439843936	38.0	35.0	40.0	30.0	41.0
36	35.95747585534208	38.0	35.0	40.0	30.0	41.0
37	35.88626112896967	38.0	35.0	40.0	30.0	41.0
38	35.777043262812775	38.0	34.0	40.0	29.0	41.0
39	35.75268501411479	38.0	34.0	40.0	29.0	41.0
40	35.59630274357359	38.0	34.0	40.0	29.0	41.0
41	35.69428039485816	38.0	34.0	40.0	29.0	41.0
42	35.60841690072657	38.0	34.0	40.0	29.0	41.0
43	35.60211063290508	38.0	34.0	40.0	29.0	41.0
44	35.5765509360616	38.0	34.0	40.0	29.0	41.0
45	35.37526298364963	38.0	34.0	40.0	28.0	41.0
46	35.34111337754663	38.0	34.0	40.0	28.0	41.0
47	35.22235690307606	37.0	34.0	40.0	28.0	41.0
48	35.23788673280861	37.0	34.0	40.0	28.0	41.0
49	35.145699171623754	37.0	34.0	40.0	27.0	41.0
50	35.04578496392096	37.0	34.0	40.0	27.0	41.0
51	33.818164828006026	36.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	5.0
10	14.0
11	10.0
12	14.0
13	26.0
14	38.0
15	66.0
16	105.0
17	173.0
18	343.0
19	655.0
20	1051.0
21	1535.0
22	2121.0
23	2697.0
24	3219.0
25	3774.0
26	4195.0
27	5228.0
28	6590.0
29	8550.0
30	11371.0
31	15473.0
32	21442.0
33	30837.0
34	48764.0
35	55881.0
36	67659.0
37	92571.0
38	111728.0
39	65683.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.37264471665403	33.08788192701603	20.456301106044265	26.08317225028568
2	24.452228641811818	31.846563502319242	19.85664498720235	23.84456286866659
3	20.49688335451442	31.608587773351704	21.263318275183245	26.631210596950634
4	21.08870781137086	32.32376090647928	19.21836453538665	27.369166746763206
5	18.091495171068416	34.59173902054387	18.697914997988686	28.61885081039902
6	20.19483039112032	36.47899156672398	20.875473014584692	22.45070502757101
7	80.34359637038065	7.584430655972889	6.895244401251642	5.176728572394816
8	82.06051026837682	5.563327886768407	5.787242222625672	6.588919622229104
9	76.34944875779162	8.56481234269929	9.85080683917682	5.234932060332276
10	34.150318072272	35.353902125584256	13.610716561473208	16.885063240670533
11	26.705433393494737	26.678022576545597	27.201141998711336	19.41540203124833
12	27.083133091975753	24.714233333689318	29.894165767805465	18.30846780652947
13	24.55368426298721	30.05346889228261	25.763676039742123	19.629170804988057
14	18.41544118955826	31.369010113523498	29.98387389600265	20.23167480091559
15	16.691407598848034	30.717380237868934	30.68284972820573	21.908362435077304
16	19.354172673907392	29.189850166066833	29.649782315395267	21.806194844630504
17	18.685099551103374	28.646617611983867	28.89651882624746	23.7717640106653
18	21.7543990801357	27.812367618213596	26.791047698381337	23.642185603269365
19	21.52870482109992	31.909216798202987	26.215954519402946	20.346123861294146
20	23.864320015948113	29.34719537504761	26.39590475275087	20.392579856253406
21	21.914592166202105	30.128937635051674	26.763814873750047	21.192655324996174
22	20.86052165988516	27.946039848920122	27.6870610264461	23.50637746474862
23	20.80125021804059	30.710438537472722	23.948154397656197	24.54015684683049
24	18.39390411909822	30.295894429196434	28.421991306855197	22.88821014485015
25	19.85183919462036	29.14517409428609	27.3111412511436	23.69184545994995
26	21.555403668777657	32.233518801328536	24.437811264065843	21.773266265827967
27	19.41397809270552	29.564346002826518	26.545062315110478	24.47661358935748
28	20.952365695896567	29.315868727105737	29.015061709936603	20.7167038670611
29	19.81392683091798	31.382003552726662	26.679624507406256	22.124445108949097
30	23.119422165739326	28.326765416804612	26.973489824179186	21.580322593276875
31	26.128204306702123	29.690898540818978	22.8314305954555	21.3494665570234
32	23.402251958805458	28.93852501326043	24.652825984030528	23.006397043903583
33	23.870549747072918	28.162122522791915	25.96142550487521	22.00590222525996
34	21.481002879915703	28.265358067145822	26.029596562612355	24.224042490326116
35	21.242137189358907	30.047595145793508	27.065867837144147	21.644399827703438
36	24.958972770735215	29.784522500008897	25.899306185945015	19.357198543310872
37	23.297414483590888	30.476022654862216	23.51314117282698	22.713421688719915
38	23.320909469547292	29.97497428011007	25.428338512909782	21.27577773743285
39	21.730548109543594	27.513696508858676	25.72540769140404	25.030347690193693
40	23.922345511567723	28.57987049278953	24.481775366575178	23.01600862906757
41	22.129784878484646	25.698708843726305	29.081630836813083	23.08987544097597
42	24.060467550220533	28.74291145594156	24.76994492917686	22.42667606466105
43	21.64831565869617	27.558906557592977	26.822196354005364	23.970581429705494
44	23.305068153258503	25.293954312931856	25.949322027261303	25.451655506548338
45	24.559913994112016	27.39408567126243	26.28145569237232	21.764544642253238
46	20.907689624115825	27.12531727130657	28.80307285937539	23.163920245202217
47	21.655613343728085	28.51045348882742	26.12749233743072	23.706440830013776
48	22.11412155451371	25.785213110202164	30.478336554994286	21.62232878028984
49	22.882870375314603	25.322433083788106	28.174937969677227	23.619758571220068
50	21.03940393932598	25.256931910818732	27.18565666705825	26.51800748279704
51	20.76155793115969	24.882614066376895	30.197464677424524	24.15836332503889
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3229.0
1	2604.0
2	1979.0
3	1392.0
4	805.0
5	1184.5
6	1564.0
7	1424.0
8	1284.0
9	1214.5
10	1145.0
11	1138.5
12	1132.0
13	1193.5
14	1255.0
15	1338.5
16	1422.0
17	1471.5
18	1521.0
19	1729.5
20	1938.0
21	2030.5
22	2123.0
23	2397.5
24	2672.0
25	2844.5
26	3719.0
27	4421.0
28	6045.5
29	7670.0
30	8293.5
31	8917.0
32	10390.5
33	11864.0
34	12970.5
35	14077.0
36	16257.0
37	18437.0
38	19228.0
39	20019.0
40	22982.0
41	25945.0
42	29464.0
43	32983.0
44	40028.5
45	47074.0
46	50813.5
47	54553.0
48	57945.0
49	61337.0
50	57134.0
51	52931.0
52	48057.5
53	43184.0
54	37296.0
55	31408.0
56	28129.0
57	24850.0
58	22209.5
59	19569.0
60	17809.0
61	16049.0
62	14144.0
63	12239.0
64	10723.0
65	9207.0
66	7684.5
67	6162.0
68	5252.5
69	4343.0
70	3974.0
71	3605.0
72	2915.0
73	2225.0
74	1850.0
75	1179.0
76	883.0
77	720.5
78	558.0
79	398.0
80	238.0
81	247.0
82	256.0
83	174.5
84	93.0
85	61.0
86	29.0
87	47.0
88	65.0
89	48.5
90	32.0
91	23.0
92	14.0
93	8.5
94	3.0
95	3.0
96	3.0
97	4.0
98	5.0
99	9.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	561822.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.13929627120497
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.63743088776432	26.504872548125864
2	9.202170117261502	6.283112239424388
3	3.2661359649560637	3.3451075010901894
4	1.5991516024239218	2.183756413508898
5	0.9732056995670381	1.6612278855172202
6	0.7038198041104228	1.4416747688440301
7	0.5511604713535393	1.317136143716082
8	0.43814287732636203	1.1966311598530308
9	0.3865975503625457	1.187835147859413
>10	4.6748155590261256	37.09120046354457
>50	0.5054936830661546	10.86648560985767
>100	0.05453458821460433	3.4414147932307357
>500	0.004719339364725374	1.107396582965321
>1k	0.002621855202625208	2.3721487424625716
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGCT	4353	0.7748005596078473	Illumina Single End Adapter 1 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2747	0.48894489713823946	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCC	2199	0.3914051069555838	No Hit
AATCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCT	1979	0.35224679702824024	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGT	1973	0.3511788431211309	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	930	0.16553285560195222	No Hit
AAACTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCT	906	0.16126103997351474	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	775	0.13794404633496019	No Hit
CCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGC	695	0.12370466090683525	Illumina Single End Adapter 1 (95% over 21bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	667	0.11872087600699154	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTT	590	0.1050154675324213	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7799231785156153E-4	0.0	0.0	0.9289419068672996	0.0
2	1.7799231785156153E-4	0.0	0.0	1.2701531801887431	0.0
3	1.7799231785156153E-4	0.0	0.0	1.7363150606419826	0.0
4	1.7799231785156153E-4	0.0	0.0	3.76151165315705	0.0
5	1.7799231785156153E-4	0.0	0.0	4.084745702375486	0.0
6	1.7799231785156153E-4	0.0	0.0	5.689168455489461	0.0
7	1.7799231785156153E-4	0.0	0.0	6.929596918596993	0.0
8	1.7799231785156153E-4	0.0	0.0	7.7339442029682	0.0
9	1.7799231785156153E-4	0.0	0.0	9.203092794514989	0.0
10	1.7799231785156153E-4	0.0	0.0	10.841868064974316	0.0
11	1.7799231785156153E-4	0.0	0.0	12.97279209429321	0.0
12	1.7799231785156153E-4	0.0	0.0	13.893012377585784	0.0
13	3.5598463570312306E-4	0.0	0.0	14.289579261759062	0.0
14	3.5598463570312306E-4	0.0	0.0	14.747909480226834	0.0
15	3.5598463570312306E-4	0.0	0.0	15.099444307983667	0.0
16	3.5598463570312306E-4	0.0	0.0	15.741996575427805	0.0
17	3.5598463570312306E-4	0.0	0.0	16.5904859546262	0.0
18	3.5598463570312306E-4	0.0	0.0	17.672679247163693	0.0
19	5.339769535546846E-4	0.0	0.0	18.154148466952165	0.0
20	5.339769535546846E-4	0.0	0.0	18.662494526736225	0.0
21	5.339769535546846E-4	0.0	0.0	19.18828383366974	0.0
22	5.339769535546846E-4	0.0	0.0	19.76408898191954	0.0
23	5.339769535546846E-4	0.0	0.0	20.361253208311528	0.0
24	5.339769535546846E-4	0.0	0.0	20.790748671287346	0.0
25	5.339769535546846E-4	0.0	0.0	21.141215545137072	0.0
26	5.339769535546846E-4	0.0	0.0	21.46480557899121	0.0
27	5.339769535546846E-4	0.0	0.0	21.82612998422988	0.0
28	5.339769535546846E-4	0.0	0.0	22.22963856879937	0.0
29	5.339769535546846E-4	0.0	0.0	22.614991936948	0.0
30	5.339769535546846E-4	0.0	0.0	23.139891282292258	0.0
31	5.339769535546846E-4	0.0	0.0	23.4980118258096	0.0
32	5.339769535546846E-4	0.0	0.0	23.869125808530104	0.0
33	5.339769535546846E-4	0.0	0.0	24.260352923167837	0.0
34	5.339769535546846E-4	0.0	0.0	24.603344119667796	0.0
35	5.339769535546846E-4	0.0	0.0	24.996351157484042	0.0
36	5.339769535546846E-4	0.0	0.0	25.337028453851932	0.0
37	5.339769535546846E-4	0.0	0.0	25.683935481344626	0.0
38	5.339769535546846E-4	0.0	0.0	26.011085361555796	0.0
39	5.339769535546846E-4	0.0	0.0	26.390564983215324	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCCG	20	7.030205E-4	45.0	1
CTATACG	25	3.8882514E-5	45.0	1
GCCGGAT	20	7.030205E-4	45.0	20
CGATTGA	20	7.030205E-4	45.0	34
ATGTCGC	20	7.030205E-4	45.0	35
CGTTCGA	20	7.030205E-4	45.0	14
CTAACGG	75	0.0	45.0	2
ACTAGCG	35	1.2104465E-7	45.0	1
ATAACGG	85	0.0	45.0	2
ATACGAG	25	3.8882514E-5	45.0	1
ATTGCGG	35	1.2104465E-7	45.0	2
TATCGGG	25	3.8882514E-5	45.0	3
CGATATG	35	1.2104465E-7	45.0	1
TCAACGT	20	7.030205E-4	45.0	15
ATCGAAT	20	7.030205E-4	45.0	32
TCTATCG	20	7.030205E-4	45.0	37
ACGCATG	30	2.1635133E-6	44.999996	1
TAGCACG	30	2.1635133E-6	44.999996	1
TATTAGG	30	2.1635133E-6	44.999996	2
TAACGGG	240	0.0	43.124996	3
>>END_MODULE