FastQCFastQC Report
Sat 18 Jun 2016
SRR3552869_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552869_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345647
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGCT31320.9061267709541816Illumina Paired End PCR Primer 2 (95% over 23bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCC31110.9000512083136841No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGT26190.7577094550220311No Hit
AATCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCT24220.7007148912040319RNA PCR Primer, Index 23 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20850.6032165764493833No Hit
AAACTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCT7800.22566375521847434RNA PCR Primer, Index 23 (95% over 21bp)
AATGACTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTT6350.18371344174837334No Hit
AAAAACTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTT5770.16693331636033296No Hit
AATGATCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCT5040.14581350337193727No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5020.14523487835855656No Hit
AACTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTG4910.1420524407849627RNA PCR Primer, Index 1 (95% over 21bp)
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG4360.12614025291699335No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGCAGGATATCGTAT4300.12440437787685124No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4270.12353644035678019No Hit
CCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC3930.11369981512930821RNA PCR Primer, Index 1 (95% over 22bp)
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3820.11051737755571435No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCAGGATATC3790.10964944003564331No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG253.8851253E-545.0000041
TATGCCA502.1827873E-1145.00000410
TCTAGCG253.8851253E-545.0000041
CGGGCTA253.8851253E-545.0000046
CGTAATG253.8851253E-545.0000041
TATAGCG253.8851253E-545.0000041
CAGTACG253.8851253E-545.0000041
ACGCGAG207.026433E-445.01
AGACTCG207.026433E-445.038
AACCGCG351.2086275E-745.01
CTGTCCG207.026433E-445.01
GCGCGTA207.026433E-445.023
TAAACCG406.7921064E-945.01
ATTAACG207.026433E-445.01
TAACGTA207.026433E-445.013
TGACGTG207.026433E-445.020
TAACGCG207.026433E-445.01
CGACAAT351.2086275E-745.020
ACGGGTA700.045.05
CGTTCCT207.026433E-445.038
Produced by FastQC (version 0.11.5)