##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552869_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	345647
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.098380717900053	31.0	31.0	33.0	28.0	34.0
2	31.369203262287826	31.0	31.0	34.0	30.0	34.0
3	31.56789441250756	31.0	31.0	34.0	30.0	34.0
4	35.510882489939156	37.0	35.0	37.0	33.0	37.0
5	34.84892679525643	35.0	35.0	37.0	32.0	37.0
6	34.99854765121641	35.0	35.0	37.0	32.0	37.0
7	34.98681602907012	36.0	35.0	37.0	32.0	37.0
8	35.40659111752742	37.0	35.0	37.0	33.0	37.0
9	36.87562455337382	39.0	37.0	39.0	33.0	39.0
10	36.25089469892694	38.0	35.0	39.0	32.0	39.0
11	36.456862058689936	38.0	35.0	39.0	32.0	39.0
12	36.13160825929344	37.0	35.0	39.0	32.0	39.0
13	35.78257875809713	37.0	35.0	39.0	30.0	39.0
14	36.82824963040327	39.0	35.0	40.0	31.0	41.0
15	36.87245658142556	38.0	35.0	40.0	32.0	41.0
16	36.76171354011463	38.0	35.0	40.0	31.0	41.0
17	36.70718102572856	38.0	35.0	40.0	31.0	41.0
18	36.63939510541101	38.0	35.0	40.0	31.0	41.0
19	36.71926560913302	38.0	35.0	40.0	31.0	41.0
20	36.63148240835303	38.0	35.0	40.0	31.0	41.0
21	36.75257994427841	38.0	35.0	40.0	31.0	41.0
22	36.74364308094675	39.0	35.0	40.0	31.0	41.0
23	36.69691621799119	38.0	35.0	40.0	31.0	41.0
24	36.182709527350156	38.0	35.0	40.0	30.0	41.0
25	36.446116992191456	38.0	35.0	40.0	31.0	41.0
26	36.31667568357312	38.0	35.0	40.0	30.0	41.0
27	36.19130789504899	38.0	35.0	40.0	30.0	41.0
28	36.30994627466751	38.0	35.0	40.0	30.0	41.0
29	35.87975014971922	38.0	34.0	40.0	29.0	41.0
30	35.84681192083253	38.0	34.0	40.0	29.0	41.0
31	35.857154842946706	38.0	34.0	40.0	30.0	41.0
32	35.76482075643648	38.0	34.0	40.0	29.0	41.0
33	35.66270501407505	38.0	34.0	40.0	29.0	41.0
34	35.680005901975136	38.0	34.0	40.0	29.0	41.0
35	35.51348051624924	38.0	34.0	40.0	28.0	41.0
36	35.56585186621032	38.0	34.0	40.0	29.0	41.0
37	35.36367739341004	38.0	34.0	40.0	28.0	41.0
38	35.25021192141115	38.0	34.0	40.0	27.0	41.0
39	35.26029156914424	37.0	34.0	40.0	27.0	41.0
40	35.01997702858697	37.0	34.0	40.0	26.0	41.0
41	35.171064698955874	37.0	34.0	40.0	27.0	41.0
42	35.121846855317706	37.0	34.0	40.0	27.0	41.0
43	35.09635263722816	37.0	34.0	40.0	27.0	41.0
44	34.98338767586584	37.0	34.0	40.0	27.0	41.0
45	34.79389955648393	37.0	33.0	40.0	26.0	41.0
46	34.76239631763041	37.0	33.0	40.0	26.0	41.0
47	34.651786360072556	37.0	33.0	40.0	26.0	41.0
48	34.68671795213035	37.0	33.0	40.0	26.0	41.0
49	34.5786336927559	36.0	33.0	40.0	26.0	41.0
50	34.42493642357665	36.0	33.0	40.0	26.0	41.0
51	33.16188481311858	35.0	31.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	2.0
11	9.0
12	5.0
13	17.0
14	19.0
15	38.0
16	73.0
17	151.0
18	260.0
19	461.0
20	792.0
21	1097.0
22	1446.0
23	1931.0
24	2364.0
25	2698.0
26	3270.0
27	3904.0
28	4950.0
29	6368.0
30	8609.0
31	11404.0
32	15312.0
33	21422.0
34	33502.0
35	35939.0
36	39565.0
37	52471.0
38	61298.0
39	36265.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.118213090233677	34.372350982360615	19.78405714500632	25.725378782399382
2	22.86841777883216	35.12224899970201	18.841187685702465	23.168145535763365
3	21.106793925594612	30.882663526661595	22.643621961133757	25.366920586610036
4	21.678764751321435	31.49455947831169	19.764383894551376	27.062291875815497
5	17.933614352214832	36.064539833992484	17.797058849056985	28.2047869647357
6	19.88126614725428	35.589488698006924	22.70061652495176	21.828628629787037
7	77.43680691572617	9.189722462512332	7.636982239105214	5.736488382656294
8	79.52361802648366	5.85655307293279	5.9459506375001085	8.673878263083434
9	75.1567350504995	8.399031381727601	10.769947374055032	5.674286193717868
10	38.22975463406307	31.112956281987113	13.88497513359005	16.77231395035976
11	29.27466461447662	24.9902357028992	26.72379624298779	19.011303439636393
12	29.785590501291782	22.17609295032215	30.29044082546644	17.747875722919627
13	24.53861887995556	30.24501876191606	25.52372796523621	19.69263439289217
14	18.110673606309327	30.376077327446787	31.28365066093442	20.229598405309464
15	17.28960471232211	28.66710835042688	30.58669683231736	23.456590104933646
16	18.058886667611755	26.622247553139474	32.80514513361898	22.51372064562979
17	17.813838974445027	26.70412299253284	28.48165903363837	27.000378999383763
18	21.05732148695056	27.06315981333557	27.28448388095369	24.59503481876018
19	22.101739636102728	31.311424661576698	27.297502943754754	19.28933275856582
20	24.92166863881359	29.679991436349802	24.67633163314017	20.722008291696444
21	20.982100235211067	29.254123426501604	26.628323115779974	23.135453222507355
22	20.074816214230125	27.82897001854493	27.133752064968014	24.962461702256928
23	21.685708251482	28.013551397813373	24.378050438742417	25.922689911962205
24	18.946208125631063	30.47617945476165	27.642363451729658	22.935248967877634
25	20.39161340905606	27.48237363552989	27.016291187251735	25.10972176816232
26	21.586763374193904	31.669882857366037	23.825752863470534	22.91760090496952
27	18.178951357888252	29.16154342436069	27.15892225305008	25.50058296470098
28	23.543673169447445	26.74057636837583	27.880467644735813	21.835282817440916
29	20.54089866250828	28.0667848990444	27.430586696832314	23.961729741615002
30	23.568554045022815	29.389232367126	24.814912323844847	22.227301264006343
31	28.521873472068325	26.91387455988335	22.235980639207053	22.32827132884127
32	26.56062398921443	27.925600395779508	22.337240016548677	23.176535598457384
33	26.08962322832254	28.406148469392186	23.64175010921547	21.86247819306981
34	22.39915289298041	26.732475618188502	25.784398533764215	25.083972955066873
35	23.256964475317304	30.0549404450205	24.646532444951063	22.041562634711138
36	24.673438508073264	27.144745940222247	27.141274190141967	21.04054136156252
37	22.796089652159573	31.21016528423507	24.08584480698516	21.907900256620195
38	25.308768772765276	30.355246826965082	24.41971143970583	19.916272960563813
39	23.658819547110202	26.783683931872694	27.225464129588854	22.33203239142825
40	24.104650119920034	29.29260198989142	23.742141549037026	22.86060634115152
41	20.468859848342383	24.833139011766338	30.959042028427845	23.73895911146343
42	25.04896614175734	26.715695492800457	24.135895870642592	24.099442494799607
43	22.532525958564662	25.3651847115699	27.38285013322841	24.71943919663703
44	22.542362583792137	25.073557704826023	26.336985421542785	26.047094289839055
45	25.439538025789314	27.61169632602048	25.220528458224724	21.728237189965487
46	21.375275931803255	25.81622290950015	30.924324527625004	21.884176631071583
47	22.294711078065195	27.143299377688795	25.670120093621527	24.891869450624483
48	22.301365265719074	23.545409044487585	32.281200184002756	21.87202550579059
49	23.27056216313175	23.25436066275709	29.253255488981534	24.221821685129626
50	21.241902866219004	25.107985893122176	26.473830237207324	27.176281003451496
51	20.356606595746527	24.55337381779677	31.07968534371772	24.010334242738978
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2414.0
1	1936.5
2	1459.0
3	996.0
4	533.0
5	692.5
6	852.0
7	764.0
8	676.0
9	656.5
10	637.0
11	613.5
12	590.0
13	660.5
14	731.0
15	703.0
16	675.0
17	785.0
18	895.0
19	897.5
20	900.0
21	907.5
22	915.0
23	1242.0
24	1569.0
25	1848.5
26	2357.5
27	2587.0
28	3320.0
29	4053.0
30	4419.0
31	4785.0
32	5422.5
33	6060.0
34	7230.0
35	8400.0
36	9201.0
37	10002.0
38	10420.0
39	10838.0
40	12729.0
41	14620.0
42	18053.5
43	21487.0
44	26632.5
45	31778.0
46	32656.0
47	33534.0
48	34389.5
49	35245.0
50	32703.5
51	30162.0
52	27314.5
53	24467.0
54	21746.0
55	19025.0
56	17047.5
57	15070.0
58	14048.5
59	13027.0
60	12044.0
61	11061.0
62	10070.0
63	9079.0
64	8126.5
65	7174.0
66	5874.5
67	4575.0
68	4490.0
69	4405.0
70	3792.0
71	3179.0
72	2684.5
73	2190.0
74	1878.0
75	1185.5
76	805.0
77	704.0
78	603.0
79	498.0
80	393.0
81	303.5
82	214.0
83	194.5
84	175.0
85	103.0
86	31.0
87	39.5
88	48.0
89	33.5
90	19.0
91	14.0
92	9.0
93	5.5
94	2.0
95	2.5
96	3.0
97	2.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	345647.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.788621203796936
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.21125136997578	23.156507286937376
2	9.847362058402165	6.063734005455726
3	3.287852546533486	3.0368533988747592
4	1.610790264890957	1.9837604521796555
5	1.0459810017982358	1.610215642536696
6	0.7638794255581368	1.4111276567330084
7	0.5875714760975145	1.2663360925405538
8	0.5228340876914102	1.2877872542690862
9	0.44615092222620506	1.2362734569732563
>10	6.312833516979001	45.369252817073445
>50	0.30374100165304097	5.679435198230221
>100	0.04979360682836738	2.88034412633886
>500	0.004979360682836737	0.9192326688060359
>1k	0.004979360682836737	4.099139943051332
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGCT	3132	0.9061267709541816	Illumina Paired End PCR Primer 2 (95% over 23bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCC	3111	0.9000512083136841	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGT	2619	0.7577094550220311	No Hit
AATCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCT	2422	0.7007148912040319	RNA PCR Primer, Index 23 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2085	0.6032165764493833	No Hit
AAACTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCT	780	0.22566375521847434	RNA PCR Primer, Index 23 (95% over 21bp)
AATGACTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTT	635	0.18371344174837334	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTT	577	0.16693331636033296	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCT	504	0.14581350337193727	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	502	0.14523487835855656	No Hit
AACTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTG	491	0.1420524407849627	RNA PCR Primer, Index 1 (95% over 21bp)
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	436	0.12614025291699335	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGCAGGATATCGTAT	430	0.12440437787685124	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	427	0.12353644035678019	No Hit
CCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC	393	0.11369981512930821	RNA PCR Primer, Index 1 (95% over 22bp)
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	382	0.11051737755571435	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCAGGATATC	379	0.10964944003564331	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.8931250669035174E-4	0.0	0.0	1.1306332761458946	0.0
2	2.8931250669035174E-4	0.0	0.0	1.455241908652469	0.0
3	2.8931250669035174E-4	0.0	0.0	1.919009856877103	0.0
4	2.8931250669035174E-4	0.0	0.0	4.339398287848585	0.0
5	2.8931250669035174E-4	0.0	0.0	4.686573295877007	0.0
6	2.8931250669035174E-4	0.0	0.0	6.376158334948662	0.0
7	2.8931250669035174E-4	0.0	0.0	7.634089114038311	0.0
8	2.8931250669035174E-4	0.0	0.0	8.360263505831094	0.0
9	2.8931250669035174E-4	0.0	0.0	10.206945236035608	0.0
10	2.8931250669035174E-4	0.0	0.0	11.652929144473987	0.0
11	2.8931250669035174E-4	0.0	0.0	14.197432640815629	0.0
12	2.8931250669035174E-4	0.0	0.0	15.023419847416584	0.0
13	2.8931250669035174E-4	0.0	0.0	15.401261981154184	0.0
14	2.8931250669035174E-4	0.0	0.0	15.907848180368989	0.0
15	2.8931250669035174E-4	0.0	0.0	16.22117362511464	0.0
16	2.8931250669035174E-4	0.0	0.0	16.842327576978825	0.0
17	2.8931250669035174E-4	0.0	0.0	17.538124155569122	0.0
18	2.8931250669035174E-4	0.0	0.0	18.54348511631809	0.0
19	2.8931250669035174E-4	0.0	0.0	19.00291337694237	0.0
20	2.8931250669035174E-4	0.0	0.0	19.367157822865526	0.0
21	2.8931250669035174E-4	0.0	0.0	19.850888334051792	0.0
22	2.8931250669035174E-4	0.0	0.0	20.343298220438772	0.0
23	2.8931250669035174E-4	0.0	0.0	20.855670669787383	0.0
24	2.8931250669035174E-4	0.0	0.0	21.208342615442923	0.0
25	2.8931250669035174E-4	0.0	0.0	21.526007747788928	0.0
26	2.8931250669035174E-4	0.0	0.0	21.800565316638075	0.0
27	5.786250133807035E-4	0.0	0.0	22.089588510821734	0.0
28	5.786250133807035E-4	0.0	0.0	22.395102517886745	0.0
29	5.786250133807035E-4	0.0	0.0	22.723472212980294	0.0
30	5.786250133807035E-4	0.0	0.0	23.12879903485348	0.0
31	5.786250133807035E-4	0.0	0.0	23.426212291731158	0.0
32	5.786250133807035E-4	0.0	0.0	23.72362554860884	0.0
33	5.786250133807035E-4	0.0	0.0	24.047076931088654	0.0
34	5.786250133807035E-4	0.0	0.0	24.341886375406123	0.0
35	5.786250133807035E-4	0.0	0.0	24.671991945539812	0.0
36	5.786250133807035E-4	0.0	0.0	24.936134264148105	0.0
37	5.786250133807035E-4	0.0	0.0	25.213584958064153	0.0
38	5.786250133807035E-4	0.0	0.0	25.50752646486155	0.0
39	5.786250133807035E-4	0.0	0.0	25.773693971016673	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGTAG	25	3.8851253E-5	45.000004	1
TATGCCA	50	2.1827873E-11	45.000004	10
TCTAGCG	25	3.8851253E-5	45.000004	1
CGGGCTA	25	3.8851253E-5	45.000004	6
CGTAATG	25	3.8851253E-5	45.000004	1
TATAGCG	25	3.8851253E-5	45.000004	1
CAGTACG	25	3.8851253E-5	45.000004	1
ACGCGAG	20	7.026433E-4	45.0	1
AGACTCG	20	7.026433E-4	45.0	38
AACCGCG	35	1.2086275E-7	45.0	1
CTGTCCG	20	7.026433E-4	45.0	1
GCGCGTA	20	7.026433E-4	45.0	23
TAAACCG	40	6.7921064E-9	45.0	1
ATTAACG	20	7.026433E-4	45.0	1
TAACGTA	20	7.026433E-4	45.0	13
TGACGTG	20	7.026433E-4	45.0	20
TAACGCG	20	7.026433E-4	45.0	1
CGACAAT	35	1.2086275E-7	45.0	20
ACGGGTA	70	0.0	45.0	5
CGTTCCT	20	7.026433E-4	45.0	38
>>END_MODULE