FastQCFastQC Report
Sat 18 Jun 2016
SRR3552867_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552867_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences828273
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGCT57480.6939740882535106TruSeq Adapter, Index 22 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26500.3199428207849344No Hit
AATCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCT17040.2057292704217088No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCC15570.18798149885363885No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGT13710.16552513482873402No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG13080.1579189470138469No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11230.13558331612886088No Hit
AAACTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCT10030.1210953393385997TruSeq Adapter, Index 20 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGC9750.1177148114208721TruSeq Adapter, Index 20 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGT502.1827873E-1145.028
CTATCCG406.8102963E-945.01
CGTCTAG207.032145E-445.01
ATCCGCG253.8898586E-545.01
ACGATAG406.8102963E-945.01
CCGTACC253.8898586E-545.025
CGTAGCG453.8380676E-1045.01
ACCCGTA253.8898586E-545.023
ATACGAG351.2113742E-745.01
GATTCGT207.032145E-445.09
ATAACGG850.042.352942
TACGCGG650.041.538462
TACGAAG650.041.538461
CCCAACG556.002665E-1140.9090921
ACTACGG1650.040.909092
GACCGGA501.0804797E-940.59
CTACGAA950.040.26315710
ATTGCGG950.040.2631572
ATTACCG451.9272193E-840.01
CGACTAG451.9272193E-840.01