Basic Statistics
Measure | Value |
---|---|
Filename | SRR3552867_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 828273 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 47 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGCT | 5748 | 0.6939740882535106 | TruSeq Adapter, Index 22 (95% over 22bp) |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 2650 | 0.3199428207849344 | No Hit |
AATCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCT | 1704 | 0.2057292704217088 | No Hit |
AATGATACGGCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCC | 1557 | 0.18798149885363885 | No Hit |
AATGATACCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGT | 1371 | 0.16552513482873402 | No Hit |
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG | 1308 | 0.1579189470138469 | No Hit |
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 1123 | 0.13558331612886088 | No Hit |
AAACTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCT | 1003 | 0.1210953393385997 | TruSeq Adapter, Index 20 (95% over 21bp) |
CCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGC | 975 | 0.1177148114208721 | TruSeq Adapter, Index 20 (95% over 23bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGACGGT | 50 | 2.1827873E-11 | 45.0 | 28 |
CTATCCG | 40 | 6.8102963E-9 | 45.0 | 1 |
CGTCTAG | 20 | 7.032145E-4 | 45.0 | 1 |
ATCCGCG | 25 | 3.8898586E-5 | 45.0 | 1 |
ACGATAG | 40 | 6.8102963E-9 | 45.0 | 1 |
CCGTACC | 25 | 3.8898586E-5 | 45.0 | 25 |
CGTAGCG | 45 | 3.8380676E-10 | 45.0 | 1 |
ACCCGTA | 25 | 3.8898586E-5 | 45.0 | 23 |
ATACGAG | 35 | 1.2113742E-7 | 45.0 | 1 |
GATTCGT | 20 | 7.032145E-4 | 45.0 | 9 |
ATAACGG | 85 | 0.0 | 42.35294 | 2 |
TACGCGG | 65 | 0.0 | 41.53846 | 2 |
TACGAAG | 65 | 0.0 | 41.53846 | 1 |
CCCAACG | 55 | 6.002665E-11 | 40.909092 | 1 |
ACTACGG | 165 | 0.0 | 40.90909 | 2 |
GACCGGA | 50 | 1.0804797E-9 | 40.5 | 9 |
CTACGAA | 95 | 0.0 | 40.263157 | 10 |
ATTGCGG | 95 | 0.0 | 40.263157 | 2 |
ATTACCG | 45 | 1.9272193E-8 | 40.0 | 1 |
CGACTAG | 45 | 1.9272193E-8 | 40.0 | 1 |