##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552866_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	841511
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.181673204509508	31.0	31.0	34.0	28.0	34.0
2	31.458838921891694	31.0	31.0	34.0	30.0	34.0
3	31.646693863775994	31.0	31.0	34.0	30.0	34.0
4	35.56947324515069	37.0	35.0	37.0	33.0	37.0
5	34.968852457068294	37.0	35.0	37.0	32.0	37.0
6	35.06150602903587	36.0	35.0	37.0	32.0	37.0
7	35.171973984891466	37.0	35.0	37.0	32.0	37.0
8	35.51114958687409	37.0	35.0	37.0	33.0	37.0
9	37.01972166733412	39.0	37.0	39.0	34.0	39.0
10	36.302068540993524	38.0	35.0	39.0	32.0	39.0
11	36.51977930175601	38.0	35.0	39.0	32.0	39.0
12	36.37829452021423	38.0	35.0	39.0	32.0	39.0
13	36.23487512343867	38.0	35.0	39.0	32.0	39.0
14	37.29505615494034	39.0	36.0	40.0	32.0	41.0
15	37.207152372339756	39.0	36.0	40.0	32.0	41.0
16	37.078828440745276	39.0	36.0	40.0	32.0	41.0
17	37.02848091112297	39.0	36.0	40.0	32.0	41.0
18	36.939151122207555	38.0	36.0	40.0	32.0	41.0
19	37.13315215130878	39.0	36.0	40.0	32.0	41.0
20	36.96844248025278	39.0	35.0	40.0	31.0	41.0
21	37.10742224403484	39.0	35.0	40.0	32.0	41.0
22	37.09467018256446	39.0	35.0	40.0	32.0	41.0
23	37.01982386445334	39.0	35.0	40.0	31.0	41.0
24	36.593098604771654	39.0	35.0	40.0	30.0	41.0
25	36.761538470679525	39.0	35.0	40.0	31.0	41.0
26	36.694311779643996	39.0	35.0	40.0	31.0	41.0
27	36.53546418288056	39.0	35.0	40.0	30.0	41.0
28	36.46285788302232	38.0	35.0	40.0	30.0	41.0
29	36.151500099226276	38.0	35.0	40.0	30.0	41.0
30	36.16795858877662	38.0	35.0	40.0	30.0	41.0
31	36.184020173236	38.0	35.0	40.0	30.0	41.0
32	36.160234387904616	38.0	35.0	40.0	30.0	41.0
33	36.142764622209334	38.0	35.0	40.0	30.0	41.0
34	36.104432384128074	38.0	35.0	40.0	30.0	41.0
35	36.02108944505776	38.0	35.0	40.0	30.0	41.0
36	35.99401077347771	38.0	35.0	40.0	30.0	41.0
37	35.91031370950588	38.0	35.0	40.0	29.0	41.0
38	35.860107592176455	38.0	35.0	40.0	29.0	41.0
39	35.86967252953318	38.0	35.0	40.0	29.0	41.0
40	35.68742179246617	38.0	34.0	40.0	29.0	41.0
41	35.76859244858356	38.0	35.0	40.0	29.0	41.0
42	35.686316637572176	38.0	34.0	40.0	29.0	41.0
43	35.66930913559062	38.0	34.0	40.0	29.0	41.0
44	35.55964093160993	38.0	34.0	40.0	28.0	41.0
45	35.41242716969832	38.0	34.0	40.0	28.0	41.0
46	35.34603825737275	38.0	34.0	40.0	28.0	41.0
47	35.258179631638804	38.0	34.0	40.0	27.0	41.0
48	35.26147132954887	38.0	34.0	40.0	28.0	41.0
49	35.10192736636835	37.0	34.0	40.0	27.0	41.0
50	35.01435750691316	37.0	34.0	40.0	27.0	41.0
51	33.77471120401278	36.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	8.0
10	13.0
11	20.0
12	29.0
13	36.0
14	48.0
15	75.0
16	151.0
17	267.0
18	468.0
19	883.0
20	1473.0
21	2155.0
22	3017.0
23	3867.0
24	4751.0
25	5621.0
26	6904.0
27	8706.0
28	10850.0
29	13818.0
30	18285.0
31	23926.0
32	32400.0
33	45202.0
34	68887.0
35	79973.0
36	98818.0
37	136557.0
38	169855.0
39	104446.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.060449596024295	31.633573417340948	20.64667009700408	26.659306889630674
2	24.870738469253524	30.938276505001124	19.88779706979469	24.303187955950666
3	21.28266891341884	31.190679622726265	20.69265880065739	26.83399266319751
4	20.75338290289729	31.80730852003123	19.46867004709386	27.970638529977627
5	18.41152403236559	33.32529224216915	19.2623744668816	29.000809258583665
6	20.43407632223465	36.23410745670586	20.814463506715896	22.517352714343602
7	81.26774338065694	6.2877371775294675	6.998838993192008	5.445680448621586
8	83.0067580815937	5.1414657681242435	5.901645967788894	5.950130182493158
9	76.06056248819088	8.729891825537633	10.02185354677479	5.187692139496692
10	33.45458348138052	36.11004490731553	14.138496109973605	16.296875501330348
11	26.05075869477642	28.15970320055234	27.873194765130823	17.916343339540422
12	27.225787898197407	24.360465876263053	29.85546237660589	18.558283848933645
13	25.254215334083568	28.20390939631211	26.44683194872081	20.09504332088351
14	19.065704429294446	30.328421137691603	29.206035334059806	21.399839098954143
15	17.45740697388388	30.792467359309622	30.201031240233345	21.549094426573152
16	20.280661809530713	28.83123334097831	29.021367516289153	21.866737333201826
17	19.702535082726193	27.710986546818756	28.518106121013272	24.068372249441776
18	22.00137609609381	27.464049786633804	26.955084366098603	23.57948975117378
19	22.870764612702626	31.238569668132683	26.16840421575	19.72226150341469
20	23.94407203233232	30.077919361719573	25.590871658243326	20.387136947704786
21	23.262322179983386	29.028735215582447	26.70089874047992	21.008043863954246
22	20.642510911919153	27.646459761072638	27.453354739272573	24.257674587735632
23	21.206496409434934	28.88672875339716	25.40335182784301	24.503423009324894
24	18.761846250375815	29.86116640186522	28.167070899845637	23.209916447913336
25	20.31892631231202	29.32617636608434	26.23887269447458	24.116024627129057
26	21.64594402212211	30.328539971551173	24.3336094240004	23.691906582326318
27	18.82328335577313	30.240127580031633	26.247666400082707	24.688922664112532
28	20.3089442681082	30.18855368497857	27.47628967416944	22.026212372743792
29	19.96800992500395	28.706576622290143	26.958055212587833	24.367358240118072
30	21.127709560540502	29.86580092238842	26.32134339301566	22.685146124055418
31	21.70274660699622	29.664139862699358	23.898677497976852	24.734436032327565
32	22.440229539483145	28.868666006742632	24.536102320706444	24.15500213306778
33	21.121292532123764	27.58526032339447	27.439451177702967	23.853995966778808
34	18.699101972523234	28.218050625600853	27.536538440971064	25.546308960904852
35	20.34186124720889	28.29422312958476	27.576585451645908	23.78733017156044
36	20.349110112642617	28.62172924655768	28.081867022534464	22.94729361826524
37	20.36158766789739	30.040011360516978	24.778523394227765	24.81987757735787
38	20.930683021374648	27.96933135752236	27.047655942703063	24.052329678399925
39	20.450831896433915	25.826994537207476	28.00914070047807	25.713032865880542
40	22.94218376230376	26.1970431759062	27.712174885414452	23.14859817637559
41	20.49432508903627	24.214656730571555	30.57666507033182	24.714353110060355
42	23.81561263013793	27.10766704178555	25.827351038786183	23.249369289290335
43	20.605315913873973	27.130245475103713	27.04516043165211	25.219278179370203
44	20.89740954069525	26.038399973381214	26.379690818064173	26.684499667859363
45	22.288122199234472	26.59299759599102	26.69329337346749	24.425586831307015
46	19.346152337877935	26.655979541562736	29.461528132133747	24.53633998842558
47	20.24679415955347	27.631605528626483	27.213072675223497	24.90852763659655
48	20.932227861549045	25.050534098781835	30.673633499740347	23.343604539928773
49	21.504888230813382	24.965211387611095	29.038004256628852	24.491896124946674
50	20.062126341782815	24.994325683205567	28.008784198899363	26.934763776112252
51	20.473647997471215	24.771749864232316	29.844648495385083	24.909953642911383
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3373.0
1	2656.0
2	1939.0
3	1423.0
4	907.0
5	1496.0
6	2085.0
7	1899.5
8	1714.0
9	1601.0
10	1488.0
11	1479.0
12	1470.0
13	1531.5
14	1593.0
15	1833.5
16	2074.0
17	2010.0
18	1946.0
19	1973.5
20	2001.0
21	2324.0
22	2647.0
23	3233.5
24	3820.0
25	4733.5
26	6890.5
27	8134.0
28	9079.0
29	10024.0
30	11693.5
31	13363.0
32	15920.5
33	18478.0
34	21651.0
35	24824.0
36	27270.5
37	29717.0
38	31580.0
39	33443.0
40	37402.0
41	41361.0
42	45724.0
43	50087.0
44	58335.5
45	66584.0
46	72409.0
47	78234.0
48	82768.5
49	87303.0
50	83053.0
51	78803.0
52	70249.0
53	61695.0
54	54331.0
55	46967.0
56	41683.5
57	36400.0
58	33080.5
59	29761.0
60	27810.5
61	25860.0
62	22929.0
63	19998.0
64	17580.0
65	15162.0
66	13088.5
67	11015.0
68	9131.5
69	7248.0
70	6152.5
71	5057.0
72	4291.0
73	3525.0
74	2891.0
75	1900.0
76	1543.0
77	1217.5
78	892.0
79	672.0
80	452.0
81	323.0
82	194.0
83	182.0
84	170.0
85	138.0
86	106.0
87	70.5
88	35.0
89	59.0
90	83.0
91	46.0
92	9.0
93	9.0
94	9.0
95	7.0
96	5.0
97	7.0
98	9.0
99	4.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	841511.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.23471991830597
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.87375790283075	26.65986290789376
2	8.886583572247824	6.084593992530464
3	3.3245226926341758	3.4144230973315017
4	1.636802143205288	2.241418517372639
5	1.01059373049795	1.729869665739665
6	0.6641584668404484	1.3642367456192552
7	0.5002872064813172	1.198903467481972
8	0.43069792611773583	1.1795858296028738
9	0.35376627245721615	1.0899980328704322
>10	4.749358768858001	38.29694328335817
>50	0.5144061631063287	10.858765820022702
>100	0.04809488197331557	2.9887823251597085
>500	0.004182163649853509	0.8646589026120699
>1k	0.002439595462414547	1.322222983130531
>5k	3.4851363748779243E-4	0.7057344292742367
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGCT	5915	0.7029022793522604	TruSeq Adapter, Index 22 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2800	0.33273480679396944	No Hit
AATCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCT	1850	0.21984264020315836	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCC	1638	0.19464986197447212	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGT	1357	0.16125754743550588	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1316	0.15638535919316562	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1063	0.1263203927221391	No Hit
AAACTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCT	1058	0.1257262234242927	TruSeq Adapter, Index 20 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGC	977	0.11610068079918147	TruSeq Adapter, Index 20 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.565015787078244E-4	0.0	0.0	0.8673683409961367	0.0
2	3.565015787078244E-4	0.0	0.0	1.1966569658625972	0.0
3	3.565015787078244E-4	0.0	0.0	1.6006920883981315	0.0
4	3.565015787078244E-4	0.0	0.0	3.3814174740437144	0.0
5	3.565015787078244E-4	0.0	0.0	3.721163478552271	0.0
6	3.565015787078244E-4	0.0	0.0	5.15192314776634	0.0
7	3.565015787078244E-4	0.0	0.0	6.195403268644141	0.0
8	3.565015787078244E-4	0.0	0.0	6.866458073631836	0.0
9	3.565015787078244E-4	0.0	0.0	8.024850536713126	0.0
10	3.565015787078244E-4	0.0	0.0	9.438854631727928	0.0
11	3.565015787078244E-4	0.0	0.0	11.113936716216426	0.0
12	3.565015787078244E-4	0.0	0.0	11.908341067437027	0.0
13	4.7533543827709914E-4	0.0	0.0	12.280291047888857	0.0
14	4.7533543827709914E-4	0.0	0.0	12.678859812884205	0.0
15	4.7533543827709914E-4	0.0	0.0	12.980460148471025	0.0
16	4.7533543827709914E-4	0.0	0.0	13.583660819644663	0.0
17	4.7533543827709914E-4	0.0	0.0	14.330531627037555	0.0
18	4.7533543827709914E-4	0.0	0.0	15.228677937662134	0.0
19	4.7533543827709914E-4	0.0	0.0	15.66360986368568	0.0
20	5.941692978463739E-4	0.0	0.0	16.114703194610648	0.0
21	5.941692978463739E-4	0.0	0.0	16.64173136180038	0.0
22	5.941692978463739E-4	0.0	0.0	17.229364797370444	0.0
23	5.941692978463739E-4	0.0	0.0	17.825554270829496	0.0
24	5.941692978463739E-4	0.0	0.0	18.237313594237033	0.0
25	5.941692978463739E-4	0.0	0.0	18.59963803206375	0.0
26	5.941692978463739E-4	0.0	0.0	18.941047710606277	0.0
27	5.941692978463739E-4	0.0	0.0	19.29790579089281	0.0
28	5.941692978463739E-4	0.0	0.0	19.665934253978854	0.0
29	5.941692978463739E-4	0.0	0.0	20.06616669300817	0.0
30	5.941692978463739E-4	0.0	0.0	20.536273441464225	0.0
31	5.941692978463739E-4	0.0	0.0	20.94482425066339	0.0
32	5.941692978463739E-4	0.0	0.0	21.30180116480949	0.0
33	5.941692978463739E-4	0.0	0.0	21.67375114526132	0.0
34	7.130031574156488E-4	0.0	0.0	22.04415628553875	0.0
35	7.130031574156488E-4	0.0	0.0	22.440467207202282	0.0
36	7.130031574156488E-4	0.0	0.0	22.808614504147897	0.0
37	7.130031574156488E-4	0.0	0.0	23.17937614600403	0.0
38	7.130031574156488E-4	0.0	0.0	23.540036909796783	0.0
39	9.506708765541983E-4	0.0	0.0	23.936347831460314	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGGTA	20	7.032209E-4	45.0	26
TAACGCG	35	1.2114106E-7	45.0	1
CATAGCG	50	2.1827873E-11	45.0	1
CCGAACG	35	1.2114106E-7	45.0	1
TAGTGCG	35	1.2114106E-7	45.0	1
ATGTTCG	20	7.032209E-4	45.0	15
TATCGTG	25	3.889912E-5	45.0	1
CGATACG	25	3.889912E-5	45.0	1
GCCCGTA	20	7.032209E-4	45.0	11
GCGTAAG	25	3.889912E-5	45.0	1
TCGCGCG	25	3.889912E-5	45.0	1
ATAACGG	75	0.0	42.0	2
CTATCCG	65	0.0	41.53846	1
AGTACGG	115	0.0	41.086956	2
CTAGACG	55	6.002665E-11	40.909092	1
TACTACG	55	6.002665E-11	40.909092	1
CGACGGT	50	1.0804797E-9	40.5	28
CACTGCG	45	1.9272193E-8	40.0	1
TAGCGGG	440	0.0	39.375	3
TAGCGCG	80	0.0	39.375	1
>>END_MODULE