SRR3552861_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3552861_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	305444
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGCT	3878	1.2696271656997682	TruSeq Adapter, Index 20 (95% over 23bp)
AATCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCT	2985	0.9772658817983002	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCC	2916	0.9546758161888923	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGT	2769	0.9065491546731971	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2323	0.7605322088500674	No Hit
AAACTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCT	1007	0.32968400099527245	TruSeq Adapter, Index 23 (95% over 22bp)
AAAAACTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTT	796	0.2606042351462134	No Hit
AATGACTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTT	755	0.24718115268265212	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCT	744	0.24357983787535523	No Hit
CCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGC	662	0.2167336729482327	TruSeq Adapter, Index 23 (95% over 24bp)
AACTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTG	654	0.21411453490656224	TruSeq Adapter, Index 23 (95% over 23bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	568	0.1859588009586045	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	468	0.15321957543772344	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTAT	425	0.13914170846374457	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCT	414	0.13554039365644766	No Hit
AACCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCT	399	0.1306295098283155	No Hit
ACCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTG	399	0.1306295098283155	TruSeq Adapter, Index 20 (95% over 21bp)
AAATTAGGGAATGTAAAAATAAAGTTAAGGAAAATAATGTATGTATTGGCA	398	0.1303021175731067	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTCTCTTGTC	389	0.1273555872762274	No Hit
AATGCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTC	389	0.1273555872762274	No Hit
TACCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCT	363	0.11884338864079831	TruSeq Adapter, Index 23 (95% over 22bp)
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	363	0.11884338864079831	No Hit
GCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGC	333	0.10902162098453398	TruSeq Adapter, Index 23 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGC	315	0.10312856039077539	TruSeq Adapter, Index 23 (95% over 24bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	20	7.0251425E-4	45.000004	6
CTACTCG	20	7.0251425E-4	45.000004	1
GCGAAGG	80	0.0	45.000004	2
ACCGCGC	20	7.0251425E-4	45.000004	35
ACACCGC	20	7.0251425E-4	45.000004	33
CGCATTG	20	7.0251425E-4	45.000004	1
GGACTAC	25	3.884056E-5	45.000004	8
TAGCGAG	25	3.884056E-5	45.000004	1
GATCATT	25	3.884056E-5	45.000004	9
GGTCAGC	25	3.884056E-5	45.000004	21
TGCGGGC	40	6.7866495E-9	45.000004	4
TATTGCG	20	7.0251425E-4	45.000004	1
GTTACGG	20	7.0251425E-4	45.000004	2
CTAACGG	25	3.884056E-5	45.000004	2
TCTACCG	20	7.0251425E-4	45.000004	1
CGCTAGG	20	7.0251425E-4	45.000004	2
TAACACG	25	3.884056E-5	45.000004	1
TGCGAAG	40	6.7866495E-9	45.000004	1
CGGGCAC	20	7.0251425E-4	45.000004	6
CTTTGGC	25	3.884056E-5	45.000004	25

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content