##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552861_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	305444
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.921016618430876	31.0	31.0	33.0	28.0	34.0
2	31.2151916554262	31.0	31.0	34.0	28.0	34.0
3	31.43259648249761	31.0	31.0	34.0	30.0	34.0
4	35.406784877096946	37.0	35.0	37.0	33.0	37.0
5	34.69589188198164	35.0	35.0	37.0	32.0	37.0
6	34.79971451395346	35.0	35.0	37.0	32.0	37.0
7	34.82855777163736	35.0	35.0	37.0	32.0	37.0
8	35.23226516153534	37.0	35.0	37.0	33.0	37.0
9	36.7161410929663	39.0	37.0	39.0	32.0	39.0
10	36.0193717997407	37.0	35.0	39.0	31.0	39.0
11	36.3224388103875	37.0	35.0	39.0	32.0	39.0
12	36.11431555375126	37.0	35.0	39.0	32.0	39.0
13	35.81410340356989	37.0	35.0	39.0	30.0	39.0
14	36.80400335249669	38.0	35.0	40.0	31.0	41.0
15	36.795271801050276	38.0	35.0	40.0	31.0	41.0
16	36.6545946229096	38.0	35.0	40.0	31.0	41.0
17	36.58203795131023	38.0	35.0	40.0	31.0	41.0
18	36.488862115477794	38.0	35.0	40.0	31.0	41.0
19	36.53722449941724	38.0	35.0	40.0	31.0	41.0
20	36.43322834955016	38.0	35.0	40.0	30.0	41.0
21	36.59490446693993	38.0	35.0	40.0	31.0	41.0
22	36.57091643640078	38.0	35.0	40.0	31.0	41.0
23	36.50254711174552	38.0	35.0	40.0	31.0	41.0
24	36.00700946818402	38.0	34.0	40.0	29.0	41.0
25	36.28092874634957	38.0	35.0	40.0	30.0	41.0
26	36.14415735781354	38.0	35.0	40.0	30.0	41.0
27	36.06783240135671	38.0	34.0	40.0	30.0	41.0
28	36.17464412461859	38.0	35.0	40.0	30.0	41.0
29	35.685428425505165	38.0	34.0	40.0	29.0	41.0
30	35.86136902345438	38.0	34.0	40.0	30.0	41.0
31	35.73766058590118	38.0	34.0	40.0	29.0	41.0
32	35.73433755451081	38.0	34.0	40.0	29.0	41.0
33	35.72551760715549	38.0	34.0	40.0	29.0	41.0
34	35.675135213001404	38.0	34.0	40.0	29.0	41.0
35	35.619668417123926	38.0	34.0	40.0	29.0	41.0
36	35.58739408860544	38.0	34.0	40.0	29.0	41.0
37	35.52298948416076	38.0	34.0	40.0	28.0	41.0
38	35.43048152852896	38.0	34.0	40.0	28.0	41.0
39	35.412867825198724	38.0	34.0	40.0	27.0	41.0
40	35.11592304972434	38.0	34.0	40.0	26.0	41.0
41	35.27645656814342	38.0	34.0	40.0	27.0	41.0
42	35.143387986013806	38.0	34.0	40.0	27.0	41.0
43	35.08797357289716	38.0	34.0	40.0	27.0	41.0
44	35.03094511596234	37.0	34.0	40.0	26.0	41.0
45	34.79602480323725	37.0	33.0	40.0	26.0	41.0
46	34.785322350414475	37.0	33.0	40.0	26.0	41.0
47	34.683067927345114	37.0	33.0	40.0	26.0	41.0
48	34.69956194916253	37.0	33.0	40.0	26.0	41.0
49	34.59099212949018	37.0	33.0	40.0	26.0	41.0
50	34.48349615641492	37.0	33.0	40.0	25.0	41.0
51	33.214733306268904	35.0	31.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	5.0
12	11.0
13	9.0
14	18.0
15	33.0
16	53.0
17	122.0
18	243.0
19	485.0
20	791.0
21	1117.0
22	1638.0
23	1866.0
24	2166.0
25	2524.0
26	2924.0
27	3498.0
28	4439.0
29	5666.0
30	7479.0
31	9924.0
32	13555.0
33	18581.0
34	27437.0
35	32082.0
36	36970.0
37	49301.0
38	55276.0
39	27225.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.464923193776926	36.87418970416836	19.59311690522649	25.067770196828228
2	20.234150940925343	36.88204711829337	19.54040675213787	23.343395188643417
3	19.431057738898126	32.621364309005905	23.40265318683621	24.544924765259754
4	21.502141145349064	31.378255915978055	19.651392726653658	27.468210212019223
5	16.09100195125784	37.202891528398006	20.103194038841817	26.60291248150234
6	20.697083589790598	34.32707795864381	22.93513704639803	22.04070140516756
7	73.27235106926311	10.294522072785846	9.384371603305352	7.0487552546456955
8	75.77886617514176	6.592370450884615	7.261560220531423	10.367203153442201
9	70.54124487631121	10.155380364322102	13.029229580545042	6.27414517882165
10	32.00652165372376	35.75778211390631	15.06953811500635	17.166158117363576
11	23.45241680962795	23.93139167899844	33.65592383546575	18.960267675907858
12	24.877555296551904	22.967548879663703	33.708961380809576	18.445934442974817
13	22.086208928641586	30.516559500268464	27.416482235696233	19.98074933539372
14	14.679941331307866	31.83202158169746	34.756289205222565	18.731747881772108
15	13.044944408795065	31.10357381385786	32.89506423436047	22.956417542986603
16	15.297730516886892	28.478215319338407	33.08855305718888	23.135501106585824
17	13.996673694687079	29.972433572111417	30.67305299825827	25.357839734943234
18	16.68063540288891	29.158863817917524	28.121030368905593	26.039470410287972
19	18.49209675095926	33.69979439766373	28.987637668443316	18.820471182933694
20	20.529458755123688	31.860177315645423	26.91066120139862	20.69970272783227
21	16.562774191013737	32.557522819240184	27.221029059336573	23.658673930409503
22	16.15811736357565	30.969670381477453	27.991710428098116	24.880501826848782
23	18.75106402482943	30.75882976912298	23.442922434226894	27.04718377182069
24	14.762771571875696	33.856287895653544	27.721284425295632	23.659656107175127
25	18.856811723261874	29.800552638126792	26.872683699794397	24.469951938816937
26	20.310760728644205	32.19117088566153	25.078246748994903	22.419821636699364
27	15.419520435824571	30.834129987821008	27.10284045520619	26.643509121148227
28	21.78107934678697	27.53859954688912	29.648642631709905	21.031678474614004
29	17.94698864603659	28.355770615890307	29.986838831340606	23.710401906732496
30	21.336480664213408	27.170610652034416	26.38650620080931	25.106402482942862
31	22.445685624860857	28.446130878327942	27.854533073165623	21.253650423645578
32	22.104542894933278	25.449836958656906	24.31476800984796	28.130852136561856
33	21.85572478097458	24.715823522478754	29.70004321577769	23.728408480768977
34	17.573106690588126	24.39497911237412	30.919906758685716	27.112007438352038
35	22.319312214350255	22.97638847055434	30.12139704823143	24.582902266863975
36	19.813779285237228	22.898141721559433	36.33988554366758	20.948193449535758
37	18.44233312816752	25.771336153271957	28.971922840193294	26.81440787836723
38	20.957033040426396	24.984285171749974	32.46585298778172	21.592828800041904
39	18.55004518013122	23.011092049606475	33.16909155197025	25.269771218292057
40	22.81236495069473	24.810112491978888	29.456790770157543	22.920731787168844
41	20.353649114076557	20.99762968007229	35.50143397807781	23.14728722777334
42	25.953693639423264	24.122261363785178	26.855331910268333	23.068713086523225
43	20.229567449352416	23.132881968544154	30.95002684616493	25.6875237359385
44	21.007451447728553	22.53015282670473	28.696258561307474	27.76613716425924
45	23.774570788753422	25.584395175547726	28.3220492136038	22.318984822095047
46	18.111012165896202	23.79945260014929	34.33788190306571	23.751653330888807
47	20.279003679888948	24.871662235958148	28.84849595997957	26.000838124173338
48	20.340553423868204	21.634080224198215	36.32220636188631	21.703159990047276
49	22.311127407970037	20.49835649087885	31.64868191878053	25.541834182370582
50	19.049645761579864	21.568929165411664	28.523068058302016	30.85835701470646
51	18.15619229711502	21.678605570906615	35.10921805633766	25.05598407564071
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2679.0
1	2155.5
2	1632.0
3	1146.5
4	661.0
5	801.0
6	941.0
7	877.0
8	813.0
9	828.0
10	843.0
11	919.5
12	996.0
13	1012.0
14	1028.0
15	1054.5
16	1081.0
17	1103.0
18	1125.0
19	1141.0
20	1157.0
21	1204.0
22	1251.0
23	1701.5
24	2152.0
25	2067.0
26	2121.0
27	2260.0
28	2814.0
29	3368.0
30	3674.0
31	3980.0
32	4652.5
33	5325.0
34	5998.0
35	6671.0
36	7771.5
37	8872.0
38	9408.5
39	9945.0
40	11283.5
41	12622.0
42	15754.0
43	18886.0
44	26193.5
45	33501.0
46	35758.5
47	38016.0
48	39132.5
49	40249.0
50	36244.0
51	32239.0
52	27624.5
53	23010.0
54	19384.5
55	15759.0
56	12940.5
57	10122.0
58	8493.0
59	6864.0
60	5906.5
61	4949.0
62	4306.5
63	3664.0
64	2964.0
65	2264.0
66	1792.0
67	1320.0
68	1162.0
69	1004.0
70	882.0
71	760.0
72	652.0
73	544.0
74	499.5
75	343.0
76	231.0
77	180.0
78	129.0
79	82.5
80	36.0
81	25.0
82	14.0
83	22.5
84	31.0
85	18.5
86	6.0
87	4.5
88	3.0
89	2.0
90	1.0
91	1.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	305444.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.85355733212237
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.51806481280343	33.647783920627724
2	9.419418589121584	8.07311189088362
3	3.4213607180678176	4.398524330567717
4	1.7064257955221036	2.9250566264567603
5	1.0725008830353266	2.298023903995312
6	0.7881229411485611	2.0264322985962457
7	0.5870264540676935	1.7609320263403678
8	0.49500791624894025	1.6970280095062713
9	0.4321600392195729	1.6667635515613395
>10	3.4181590803620523	28.41703547913607
>50	0.08428543105038233	2.352680218500471
>100	0.048272565056128064	4.150764918641235
>500	0.004597387148202673	1.3722052523535893
>1k	0.004597387148202673	5.2136575728332835
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGCT	3878	1.2696271656997682	TruSeq Adapter, Index 20 (95% over 23bp)
AATCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCT	2985	0.9772658817983002	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCC	2916	0.9546758161888923	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGT	2769	0.9065491546731971	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2323	0.7605322088500674	No Hit
AAACTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCT	1007	0.32968400099527245	TruSeq Adapter, Index 23 (95% over 22bp)
AAAAACTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTT	796	0.2606042351462134	No Hit
AATGACTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTT	755	0.24718115268265212	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCT	744	0.24357983787535523	No Hit
CCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGC	662	0.2167336729482327	TruSeq Adapter, Index 23 (95% over 24bp)
AACTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTG	654	0.21411453490656224	TruSeq Adapter, Index 23 (95% over 23bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	568	0.1859588009586045	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	468	0.15321957543772344	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTAT	425	0.13914170846374457	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCT	414	0.13554039365644766	No Hit
AACCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCT	399	0.1306295098283155	No Hit
ACCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTG	399	0.1306295098283155	TruSeq Adapter, Index 20 (95% over 21bp)
AAATTAGGGAATGTAAAAATAAAGTTAAGGAAAATAATGTATGTATTGGCA	398	0.1303021175731067	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTCTCTTGTC	389	0.1273555872762274	No Hit
AATGCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTC	389	0.1273555872762274	No Hit
TACCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCT	363	0.11884338864079831	TruSeq Adapter, Index 23 (95% over 22bp)
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	363	0.11884338864079831	No Hit
GCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGC	333	0.10902162098453398	TruSeq Adapter, Index 23 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGC	315	0.10312856039077539	TruSeq Adapter, Index 23 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.2739225520881076E-4	0.0	0.0	1.512224826809497	0.0
2	3.2739225520881076E-4	0.0	0.0	2.0720655832165633	0.0
3	3.2739225520881076E-4	0.0	0.0	2.9147732481240425	0.0
4	3.2739225520881076E-4	0.0	0.0	6.644098427207606	0.0
5	3.2739225520881076E-4	0.0	0.0	7.261560220531423	0.0
6	3.2739225520881076E-4	0.0	0.0	9.929152315972813	0.0
7	3.2739225520881076E-4	0.0	0.0	12.073898979845733	0.0
8	3.2739225520881076E-4	0.0	0.0	13.42930291641021	0.0
9	3.2739225520881076E-4	0.0	0.0	16.13749165149749	0.0
10	3.2739225520881076E-4	0.0	0.0	18.804428962428464	0.0
11	3.2739225520881076E-4	0.0	0.0	22.333717473579444	0.0
12	3.2739225520881076E-4	0.0	0.0	23.834156179201425	0.0
13	3.2739225520881076E-4	0.0	0.0	24.437867497806472	0.0
14	3.2739225520881076E-4	0.0	0.0	25.11818860413038	0.0
15	3.2739225520881076E-4	0.0	0.0	25.57817472269876	0.0
16	3.2739225520881076E-4	0.0	0.0	26.42644805594479	0.0
17	6.547845104176215E-4	0.0	0.0	27.572648341430835	0.0
18	6.547845104176215E-4	0.0	0.0	29.08290881470908	0.0
19	6.547845104176215E-4	0.0	0.0	29.802844383913254	0.0
20	6.547845104176215E-4	0.0	0.0	30.433074475190214	0.0
21	6.547845104176215E-4	0.0	0.0	31.123544741425597	0.0
22	6.547845104176215E-4	0.0	0.0	31.900446563036105	0.0
23	6.547845104176215E-4	0.0	0.0	32.6285669386205	0.0
24	6.547845104176215E-4	0.0	0.0	33.18447898796506	0.0
25	6.547845104176215E-4	0.0	0.0	33.61434501905423	0.0
26	9.821767656264324E-4	0.0	0.0	34.03046057542463	0.0
27	9.821767656264324E-4	0.0	0.0	34.50714369900866	0.0
28	9.821767656264324E-4	0.0	0.0	34.97105852463954	0.0
29	9.821767656264324E-4	0.0	0.0	35.445122510181896	0.0
30	9.821767656264324E-4	0.0	0.0	36.09761527481306	0.0
31	9.821767656264324E-4	0.0	0.0	36.558256177891856	0.0
32	0.001309569020835243	0.0	0.0	36.97273477298621	0.0
33	0.001309569020835243	0.0	0.0	37.40391037309622	0.0
34	0.001309569020835243	0.0	0.0	37.835740757716636	0.0
35	0.001309569020835243	0.0	0.0	38.29343513049855	0.0
36	0.0016369612760440539	0.0	0.0	38.70594937206165	0.0
37	0.0016369612760440539	0.0	0.0	39.1240292819633	0.0
38	0.0016369612760440539	0.0	0.0	39.51984651851076	0.0
39	0.0016369612760440539	0.0	0.0	39.932033367818654	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	20	7.0251425E-4	45.000004	6
CTACTCG	20	7.0251425E-4	45.000004	1
GCGAAGG	80	0.0	45.000004	2
ACCGCGC	20	7.0251425E-4	45.000004	35
ACACCGC	20	7.0251425E-4	45.000004	33
CGCATTG	20	7.0251425E-4	45.000004	1
GGACTAC	25	3.884056E-5	45.000004	8
TAGCGAG	25	3.884056E-5	45.000004	1
GATCATT	25	3.884056E-5	45.000004	9
GGTCAGC	25	3.884056E-5	45.000004	21
TGCGGGC	40	6.7866495E-9	45.000004	4
TATTGCG	20	7.0251425E-4	45.000004	1
GTTACGG	20	7.0251425E-4	45.000004	2
CTAACGG	25	3.884056E-5	45.000004	2
TCTACCG	20	7.0251425E-4	45.000004	1
CGCTAGG	20	7.0251425E-4	45.000004	2
TAACACG	25	3.884056E-5	45.000004	1
TGCGAAG	40	6.7866495E-9	45.000004	1
CGGGCAC	20	7.0251425E-4	45.000004	6
CTTTGGC	25	3.884056E-5	45.000004	25
>>END_MODULE