##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552857_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	529754
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.097903932768794	31.0	31.0	33.0	28.0	34.0
2	31.39336559988221	31.0	31.0	34.0	30.0	34.0
3	31.582102258784264	31.0	31.0	34.0	30.0	34.0
4	35.533330564752696	37.0	35.0	37.0	33.0	37.0
5	34.91080577022542	35.0	35.0	37.0	32.0	37.0
6	35.023361786791604	35.0	35.0	37.0	32.0	37.0
7	35.07270733208244	37.0	35.0	37.0	32.0	37.0
8	35.4823370847601	37.0	35.0	37.0	33.0	37.0
9	36.98473064856518	39.0	37.0	39.0	33.0	39.0
10	36.32971718948795	38.0	35.0	39.0	32.0	39.0
11	36.44253370432314	38.0	35.0	39.0	32.0	39.0
12	36.194124065132115	37.0	35.0	39.0	32.0	39.0
13	35.89013013587439	37.0	35.0	39.0	31.0	39.0
14	36.95805789102111	39.0	36.0	40.0	31.0	41.0
15	36.92558810315732	38.0	35.0	40.0	32.0	41.0
16	36.85688074087218	38.0	35.0	40.0	32.0	41.0
17	36.79218656206466	38.0	35.0	40.0	32.0	41.0
18	36.70975584894121	38.0	35.0	40.0	31.0	41.0
19	36.86625490321923	39.0	35.0	40.0	31.0	41.0
20	36.7082683660718	38.0	35.0	40.0	31.0	41.0
21	36.86285709971043	39.0	35.0	40.0	31.0	41.0
22	36.84959622768304	39.0	35.0	40.0	31.0	41.0
23	36.78813751288334	39.0	35.0	40.0	31.0	41.0
24	36.293264420844395	38.0	35.0	40.0	30.0	41.0
25	36.57478565522865	38.0	35.0	40.0	31.0	41.0
26	36.44030059235041	38.0	35.0	40.0	31.0	41.0
27	36.314304375238315	38.0	35.0	40.0	30.0	41.0
28	36.38651902581198	38.0	35.0	40.0	30.0	41.0
29	35.959841737863236	38.0	35.0	40.0	29.0	41.0
30	35.968341909641076	38.0	35.0	40.0	30.0	41.0
31	35.87000003775337	38.0	34.0	40.0	29.0	41.0
32	35.83042884055618	38.0	34.0	40.0	29.0	41.0
33	35.83431743790514	38.0	34.0	40.0	29.0	41.0
34	35.791642913503246	38.0	34.0	40.0	29.0	41.0
35	35.76835285811905	38.0	34.0	40.0	29.0	41.0
36	35.76274836999815	38.0	35.0	40.0	29.0	41.0
37	35.578508137739405	38.0	34.0	40.0	28.0	41.0
38	35.47005213740717	38.0	34.0	40.0	28.0	41.0
39	35.51529011578959	38.0	34.0	40.0	28.0	41.0
40	35.404710488264364	38.0	34.0	40.0	27.0	41.0
41	35.528484919415426	38.0	34.0	40.0	28.0	41.0
42	35.4622447400114	38.0	34.0	40.0	28.0	41.0
43	35.43250452096633	38.0	34.0	40.0	28.0	41.0
44	35.359817953238675	38.0	34.0	40.0	28.0	41.0
45	35.16516345322546	38.0	34.0	40.0	27.0	41.0
46	35.1730029409877	38.0	34.0	40.0	27.0	41.0
47	35.08579831393439	37.0	34.0	40.0	27.0	41.0
48	35.11293166262077	37.0	34.0	40.0	27.0	41.0
49	35.04267452440189	37.0	34.0	40.0	27.0	41.0
50	34.93686126013206	37.0	34.0	40.0	27.0	41.0
51	33.77863310140178	36.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	7.0
10	9.0
11	17.0
12	10.0
13	20.0
14	44.0
15	61.0
16	114.0
17	191.0
18	438.0
19	774.0
20	1207.0
21	1778.0
22	2382.0
23	2948.0
24	3471.0
25	3959.0
26	4618.0
27	5601.0
28	6980.0
29	8928.0
30	11602.0
31	15552.0
32	21432.0
33	30573.0
34	47933.0
35	53337.0
36	61007.0
37	82370.0
38	100869.0
39	61520.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.99512981497072	33.597292328137215	19.776915322961226	26.630662533930842
2	23.012190563922122	33.007584652499084	19.58437312412931	24.39585165944948
3	21.18889144772857	32.7574685608792	21.39861143096607	24.65502856042616
4	20.89328254246311	32.58493564937688	19.07111602743915	27.45066578072086
5	17.766548246922156	34.700634634188695	19.13982716506152	28.392989953827623
6	20.800409246555947	35.942343049792925	21.402575535059672	21.85467216859146
7	78.96382094330576	7.922922715071524	7.336990376665396	5.77626596495732
8	81.14558832967755	5.977868973146026	6.110383309989165	6.766159387187261
9	76.0705912555639	8.161335261272214	10.449567157586351	5.318506325577532
10	38.1443084903559	27.955617135500628	15.082472241833	18.81760213231047
11	28.436406331995606	28.996288843500945	25.353088414622636	17.214216409880812
12	28.68765502478509	25.6432230809017	28.442069337843602	17.22705255646961
13	24.101941655938415	30.33709986144512	25.172438528071524	20.38851995454494
14	18.73001430852811	31.744545581534073	30.683864586204162	18.84157552373366
15	16.941070761145742	31.878003752685206	30.29727005364754	20.88365543252151
16	19.694612971303663	28.574961208409945	30.931526708623245	20.79889911166315
17	18.79740407811928	28.957969170596165	28.803369110945837	23.441257640338723
18	19.771252317113223	28.550799050125153	27.855193165129478	23.822755467632145
19	22.307335102708052	31.70207303767409	26.737315810734795	19.253276048883066
20	23.62398396236744	30.67706897918657	26.368654130030166	19.330292928415833
21	21.158877516734183	30.30161169146434	26.844535388123543	21.694975403677933
22	19.78087942705482	28.686899957338692	28.560426160066747	22.97179445553974
23	22.12989425280413	29.18430063765446	25.101273421248354	23.584531688293055
24	18.782302729191287	31.690935792839696	27.613571582281587	21.91318989568743
25	20.175779701521837	27.99204913978941	27.22357924621617	24.608591912472583
26	21.482801451239634	32.996069873941494	23.35725638692676	22.16387228789212
27	17.15626498336964	32.37238416321538	26.40112203022535	24.07022882318963
28	19.402024335823796	29.75834066377979	27.3734601343265	23.46617486606991
29	19.082064505411946	30.613643313688993	26.833586910150746	23.47070527074831
30	19.797113377152414	31.15332777100315	26.391872454006954	22.657686397837487
31	22.872314319476587	31.95558693280277	22.770191447351035	22.401907300369604
32	21.327068790419705	31.160500911743945	23.746682422407382	23.76574787542897
33	20.86345737833032	29.700955537853417	26.495505461025303	22.940081622790956
34	20.19484515454343	29.625071259490255	24.72657120097253	25.453512384993786
35	19.047708936600763	27.703235841541545	27.411024739784885	25.83803048207281
36	22.22295631557289	28.024328273122993	28.003752685208603	21.74896272609551
37	20.135194826277857	31.746999550734866	24.54327857835901	23.57452704462826
38	21.000502119851856	30.56173242674902	26.729576369409198	21.708189083989925
39	21.203237729210162	27.49559229378164	28.283505174099677	23.01766480290852
40	22.958392008366147	26.785262593581173	26.413958176814145	23.842387221238535
41	21.236083163128548	24.26994416276234	29.5293664606591	24.964606213450015
42	23.020118772109317	26.608388044262053	25.034072418518782	25.33742076510984
43	20.632784273455226	26.44302827350053	27.701348172925545	25.2228392801187
44	21.066381754550225	25.49843889805457	26.838683615413945	26.596495731981257
45	23.158107347938856	28.780150786969045	25.758748400200847	22.30299346489125
46	20.015327869161915	26.761666735881185	30.033751514854064	23.18925388010284
47	20.399468432517732	26.731652804886796	26.809047218142762	26.05983154445271
48	20.94557096312628	24.604816575240584	32.57115566848009	21.87845679315305
49	21.91828660095063	24.000385084397664	29.61506661582546	24.466261698826248
50	20.144444402496255	24.882681395515654	27.295121886762537	27.677752315225558
51	19.929438947133953	25.32080928128905	31.43817696515741	23.311574806419582
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3764.0
1	3032.5
2	2301.0
3	1581.5
4	862.0
5	1142.5
6	1423.0
7	1282.5
8	1142.0
9	1060.0
10	978.0
11	991.5
12	1005.0
13	1030.5
14	1056.0
15	1107.5
16	1159.0
17	1246.0
18	1333.0
19	1424.5
20	1516.0
21	1561.5
22	1607.0
23	1818.5
24	2030.0
25	2926.0
26	4056.5
27	4291.0
28	5550.5
29	6810.0
30	7617.5
31	8425.0
32	10381.5
33	12338.0
34	13784.5
35	15231.0
36	17014.5
37	18798.0
38	18965.0
39	19132.0
40	21596.0
41	24060.0
42	29052.5
43	34045.0
44	41390.0
45	48735.0
46	50026.0
47	51317.0
48	54554.5
49	57792.0
50	53077.5
51	48363.0
52	42811.5
53	37260.0
54	32643.5
55	28027.0
56	25199.5
57	22372.0
58	20224.5
59	18077.0
60	15847.5
61	13618.0
62	12235.0
63	10852.0
64	9339.0
65	7826.0
66	6783.0
67	5740.0
68	4920.0
69	4100.0
70	3409.0
71	2718.0
72	2600.5
73	2483.0
74	1985.5
75	1164.5
76	841.0
77	650.0
78	459.0
79	304.0
80	149.0
81	171.0
82	193.0
83	168.0
84	143.0
85	93.5
86	44.0
87	30.5
88	17.0
89	11.0
90	5.0
91	4.0
92	3.0
93	1.5
94	0.0
95	2.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	529754.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.10477771049226
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.0721839238523	24.28417926324093
2	9.059584817285831	5.635927437820528
3	3.1841178371620247	2.971238325868145
4	1.526125864081033	1.8987922304189382
5	0.9056014533176289	1.408426594987179
6	0.5926109748958366	1.1059819585759865
7	0.4565892489870089	0.9941474966319062
8	0.37266454226878626	0.9273318198282355
9	0.33181105453834037	0.9288818183969193
>10	4.581380404513086	35.58585548349548
>50	0.8531167875613159	16.82097576201099
>100	0.05687445250408772	3.44720815532595
>500	0.0030577662636606306	0.6568375322999949
>1k	0.003669319516392756	2.3587562700607982
>5k	6.115532527321261E-4	0.9754598510380463
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGCT	5128	0.9679964662843509	Illumina Single End Adapter 1 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3243	0.6121709321685159	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCC	2090	0.3945227407438169	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGT	2046	0.38621699883342075	No Hit
AATCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCT	1938	0.36583017778063026	No Hit
AAACTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCT	1722	0.32505653567504916	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTT	1361	0.2569116986374808	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	811	0.15308992475752897	No Hit
CCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC	769	0.14516171657033264	Illumina Single End Adapter 1 (95% over 21bp)
AAAAAACTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCT	675	0.12741763157994088	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	602	0.11363765068314728	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	596	0.1125050495135478	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.7753372319982483E-4	0.0	0.0	1.2373667777874258	0.0
2	3.7753372319982483E-4	0.0	0.0	1.6390626592720394	0.0
3	3.7753372319982483E-4	0.0	0.0	2.1447690814982048	0.0
4	3.7753372319982483E-4	0.0	0.0	4.59043254038667	0.0
5	3.7753372319982483E-4	0.0	0.0	4.929646590681712	0.0
6	3.7753372319982483E-4	0.0	0.0	6.656297073736112	0.0
7	3.7753372319982483E-4	0.0	0.0	7.923111481933124	0.0
8	3.7753372319982483E-4	0.0	0.0	8.465816209032871	0.0
9	3.7753372319982483E-4	0.0	0.0	9.807759828146649	0.0
10	3.7753372319982483E-4	0.0	0.0	11.024739784881284	0.0
11	3.7753372319982483E-4	0.0	0.0	13.024913450393957	0.0
12	3.7753372319982483E-4	0.0	0.0	13.737697119795225	0.0
13	3.7753372319982483E-4	0.0	0.0	14.034249859368687	0.0
14	3.7753372319982483E-4	0.0	0.0	14.40008003714932	0.0
15	3.7753372319982483E-4	0.0	0.0	14.668884048067593	0.0
16	3.7753372319982483E-4	0.0	0.0	15.244622975947326	0.0
17	3.7753372319982483E-4	0.0	0.0	15.9492896702998	0.0
18	3.7753372319982483E-4	0.0	0.0	16.83781528785059	0.0
19	3.7753372319982483E-4	0.0	0.0	17.255933886294393	0.0
20	3.7753372319982483E-4	0.0	0.0	17.6697108469214	0.0
21	3.7753372319982483E-4	0.0	0.0	18.21468077636035	0.0
22	3.7753372319982483E-4	0.0	0.0	18.723596235233714	0.0
23	5.663005847997372E-4	0.0	0.0	19.258750287869464	0.0
24	5.663005847997372E-4	0.0	0.0	19.616463490601298	0.0
25	5.663005847997372E-4	0.0	0.0	19.944917829785144	0.0
26	5.663005847997372E-4	0.0	0.0	20.241281802497006	0.0
27	7.550674463996497E-4	0.0	0.0	20.56105286604726	0.0
28	7.550674463996497E-4	0.0	0.0	20.92461784148869	0.0
29	7.550674463996497E-4	0.0	0.0	21.23476179509735	0.0
30	7.550674463996497E-4	0.0	0.0	21.604367310109975	0.0
31	7.550674463996497E-4	0.0	0.0	21.91885290153543	0.0
32	7.550674463996497E-4	0.0	0.0	22.24164423487128	0.0
33	7.550674463996497E-4	0.0	0.0	22.58689882473752	0.0
34	7.550674463996497E-4	0.0	0.0	22.903272084778973	0.0
35	7.550674463996497E-4	0.0	0.0	23.243996269966814	0.0
36	7.550674463996497E-4	0.0	0.0	23.57207307542746	0.0
37	7.550674463996497E-4	0.0	0.0	23.885992366268116	0.0
38	9.438343079995621E-4	0.0	0.0	24.184999075042377	0.0
39	0.0011326011695994744	0.0	0.0	24.50986684385583	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCCGAC	35	1.2102646E-7	45.000004	31
CGACACG	45	3.8380676E-10	45.000004	1
ACTACCG	45	3.8380676E-10	45.000004	1
ACGTATG	35	1.2102646E-7	45.000004	1
TCGCCTC	25	3.8879483E-5	45.0	24
AGCCGGC	20	7.029841E-4	45.0	18
GCGACAA	25	3.8879483E-5	45.0	9
ATAGGCG	25	3.8879483E-5	45.0	1
CGACGGT	25	3.8879483E-5	45.0	28
TAGCGCA	20	7.029841E-4	45.0	20
TTCACTC	20	7.029841E-4	45.0	19
TCCTCGC	25	3.8879483E-5	45.0	21
TGCGGCG	20	7.029841E-4	45.0	21
TCTAGCG	40	6.8030204E-9	45.0	1
ATGTACG	25	3.8879483E-5	45.0	1
TAACCCG	20	7.029841E-4	45.0	1
TCTAACG	25	3.8879483E-5	45.0	1
CTTGACG	20	7.029841E-4	45.0	1
TATTACG	40	6.8030204E-9	45.0	1
CGGCTAA	20	7.029841E-4	45.0	5
>>END_MODULE