FastQCFastQC Report
Sat 18 Jun 2016
SRR3552852_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552852_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences327691
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40741.2432443979236536No Hit
CTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGCT36011.098901098901099No Hit
AAACTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCT14770.4507294982163074No Hit
AAAAACTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTT10040.30638619919375265No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9640.2941795777119298No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCC7760.23680845674736262No Hit
AATCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCT7690.23467229798804362No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGT7610.23223097369167905No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6980.2130055448578081No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5990.18279415669029664No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA5870.1791321702457498No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCT5520.16845137644915487No Hit
CCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC5510.16814621091210927No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG5250.16021190694892443No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3910.119319724984818No Hit
GAAAAACTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCT3700.11291124870686103No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTTA207.025896E-445.00000438
TAATACG207.025896E-445.0000041
GACAGCG207.025896E-445.0000041
ATTAACG207.025896E-445.0000041
TAGCCGT406.7902874E-945.00000444
AGAACCG207.025896E-445.0000041
CCGTAGT406.7902874E-945.00000427
CGTAGTG406.7902874E-945.00000428
AAGTTCG207.025896E-445.0000041
GCGGGTT207.025896E-445.0000045
ATGGTCG207.025896E-445.00000444
CATAAGA207.025896E-445.00000414
ATTTGCG406.7902874E-945.00000412
AGTACGG406.7902874E-945.0000042
TAGTCAT207.025896E-445.00000415
GTAGTGC406.7902874E-945.00000429
TGCGTAG207.025896E-445.0000041
CGAACAG253.884681E-545.01
TGCCACG253.884681E-545.01
GACCGAA253.884681E-545.09