##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552852_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	327691
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.28006567162357	31.0	31.0	34.0	30.0	34.0
2	31.535876786362763	31.0	31.0	34.0	30.0	34.0
3	31.74110061002591	31.0	31.0	34.0	30.0	34.0
4	35.62623630188195	37.0	35.0	37.0	35.0	37.0
5	34.98200133662505	37.0	35.0	37.0	32.0	37.0
6	35.06501856932293	36.0	35.0	37.0	32.0	37.0
7	35.05885422547461	37.0	35.0	37.0	32.0	37.0
8	35.453253217207674	37.0	35.0	37.0	33.0	37.0
9	37.03121233112902	39.0	37.0	39.0	34.0	39.0
10	36.46019268152009	38.0	35.0	39.0	32.0	39.0
11	36.580427903116046	38.0	35.0	39.0	32.0	39.0
12	36.24635708640152	38.0	35.0	39.0	32.0	39.0
13	36.02012261551278	38.0	35.0	39.0	31.0	39.0
14	37.051191518839396	39.0	36.0	40.0	32.0	41.0
15	36.99466265475707	39.0	35.0	40.0	32.0	41.0
16	36.898627060248835	38.0	35.0	40.0	32.0	41.0
17	36.81842345380252	38.0	35.0	40.0	32.0	41.0
18	36.70366290194116	38.0	35.0	40.0	31.0	41.0
19	36.793631805572936	38.0	35.0	40.0	31.0	41.0
20	36.645034498963966	38.0	35.0	40.0	31.0	41.0
21	36.7296569023867	38.0	35.0	40.0	31.0	41.0
22	36.730831789704325	39.0	35.0	40.0	31.0	41.0
23	36.61232075339268	38.0	35.0	40.0	31.0	41.0
24	36.20675880631448	38.0	35.0	40.0	30.0	41.0
25	36.39939149991913	38.0	35.0	40.0	31.0	41.0
26	36.25569515183511	38.0	35.0	40.0	30.0	41.0
27	36.144099166592916	38.0	35.0	40.0	30.0	41.0
28	36.15716025157847	38.0	35.0	40.0	30.0	41.0
29	35.719443011861784	38.0	34.0	40.0	29.0	41.0
30	35.90022307600758	38.0	34.0	40.0	30.0	41.0
31	35.788898688093354	38.0	34.0	40.0	29.0	41.0
32	35.7212282302535	38.0	34.0	40.0	29.0	41.0
33	35.70484389256952	38.0	34.0	40.0	29.0	41.0
34	35.60172845760182	38.0	34.0	40.0	28.0	41.0
35	35.48153901083643	38.0	34.0	40.0	28.0	41.0
36	35.46756548089511	38.0	34.0	40.0	28.0	41.0
37	35.337748061435924	38.0	34.0	40.0	27.0	41.0
38	35.30303853325236	38.0	34.0	40.0	27.0	41.0
39	35.327570790775454	38.0	34.0	40.0	27.0	41.0
40	35.11370467910318	38.0	34.0	40.0	27.0	41.0
41	35.165826952830564	38.0	34.0	40.0	27.0	41.0
42	35.0772618106692	37.0	34.0	40.0	27.0	41.0
43	35.05499693308635	37.0	34.0	40.0	27.0	41.0
44	34.94172864070115	37.0	34.0	40.0	26.0	41.0
45	34.7650438980625	37.0	34.0	40.0	26.0	41.0
46	34.70073331278552	37.0	33.0	40.0	25.0	41.0
47	34.55951185720694	37.0	33.0	40.0	25.0	41.0
48	34.55998181213399	37.0	33.0	40.0	25.0	41.0
49	34.44057053748806	36.0	33.0	40.0	25.0	41.0
50	34.35833758022039	36.0	33.0	40.0	24.0	41.0
51	33.14634823660094	35.0	31.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	3.0
11	11.0
12	10.0
13	11.0
14	20.0
15	43.0
16	76.0
17	150.0
18	270.0
19	594.0
20	942.0
21	1422.0
22	1889.0
23	2245.0
24	2636.0
25	2859.0
26	3248.0
27	3658.0
28	4356.0
29	5603.0
30	7267.0
31	9763.0
32	13153.0
33	18882.0
34	30163.0
35	32792.0
36	37386.0
37	51255.0
38	61405.0
39	35574.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.346912182513403	33.03630554394231	20.15099590773015	26.465786365814136
2	22.97347195986463	34.81145347293639	18.858314692805113	23.356759874393866
3	21.193136216740772	34.22248398643845	19.486650533581944	25.097729263238843
4	20.82663240674904	33.917928780466966	18.973972431345384	26.281466381438612
5	17.706314790458084	36.662282455117776	18.336786789994232	27.2946159644299
6	19.79669871922024	38.17346219456744	19.982544531280993	22.047294554931323
7	75.89436389769631	10.361895810382341	7.798810464736597	5.944929827184756
8	78.17669694926013	8.558672651980066	6.8430320027098706	6.421598396049938
9	72.38434989059816	11.493144456210272	10.91516092904596	5.207344724145613
10	36.63054523926504	32.03810907226625	13.799585585200692	17.531760103268017
11	29.292839901004296	29.146970774296516	23.289928621780884	18.270260702918296
12	29.562911401289632	26.664143964893753	26.113625336063546	17.659319297753065
13	25.08308131746066	31.247120000244134	23.190139491166985	20.479659191128228
14	18.609299614575928	33.902060172540594	27.971167960059933	19.517472252823545
15	17.16434079666515	32.20198296565972	29.01330826907056	21.62036796860457
16	18.63249219539139	29.99685679496843	28.825326298250488	22.545324711389693
17	18.356927715439237	30.008453085376164	27.441400587748827	24.193218611435775
18	21.17818310542554	28.725842333173627	25.613153855308813	24.48282070609202
19	21.333512363781733	33.32834896289492	25.097424097701797	20.240714575621546
20	24.620755528836618	31.124443454351812	24.35953382912561	19.89526718768596
21	21.854124769981475	31.673436255496796	25.34949083130144	21.12294814322029
22	20.389635357699785	29.64286477199557	25.698600205681572	24.268899664623074
23	21.703372994680965	31.363388069858495	23.060138972385573	23.87309996307497
24	18.765544369543257	31.13024159955568	27.115178628647108	22.98903540225395
25	20.98989596906842	30.10732671937893	26.09897739028536	22.80379992126729
26	21.690861207662095	33.68630813784937	22.566381133445837	22.05644952104269
27	17.68373254071671	31.476909649639445	26.063578187988075	24.775779621655765
28	20.418015752645022	29.78446158118471	28.99866032329237	20.798862342877893
29	19.769538986423186	32.92278396416136	25.10902038810953	22.198656661305925
30	20.306325166086346	28.82776762254685	29.47014107802778	21.39576613333903
31	24.84322120534284	30.68714123976551	21.804077621906004	22.665559932985648
32	21.86083841179648	29.712747679979007	24.51364242533362	23.912771482890893
33	22.309736916790513	27.770063871146906	27.41363052387768	22.506568688184906
34	19.046906994699274	29.160703223463567	25.506651082879912	26.28573869895725
35	18.90805667534354	29.42436624747094	29.292229569930207	22.37534750725531
36	22.797391444989334	28.963566286532128	28.528705396242195	19.710336872236343
37	20.257498680159053	31.717380092831355	24.13004934526734	23.89507188174225
38	22.06865614252451	29.376150092617742	26.227757246918593	22.327436517939155
39	21.05123424201458	25.60857637225313	27.721542550756656	25.618646834975635
40	22.674714899097015	27.14447452020348	25.34247202394939	24.83833855675011
41	19.544326820083555	24.61373672148457	30.076810165674374	25.765126292757508
42	23.418708478414114	27.892740417039224	25.230781437390714	23.457769667155947
43	21.29292534735467	26.34280465438477	27.52654177258454	24.837728225676017
44	21.887387813519442	24.786460415452364	26.344635647607046	26.98151612342115
45	24.35190469069947	26.316560418198854	26.199376851973355	23.132158039128324
46	20.24620755528837	26.173437781324477	29.873875083539065	23.70647957984809
47	20.54435428498189	27.388912115376986	26.50423722348188	25.56249637615925
48	21.28621170553967	24.57192904290933	31.419538528674877	22.722320722876123
49	22.276473873252545	23.868522480019287	29.39476519037752	24.46023845635065
50	19.946534997909616	24.737328763988025	27.391048274136303	27.925087963966057
51	19.680735815142924	24.047349484727988	32.05123119035921	24.220683509769874
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4635.0
1	3600.0
2	2565.0
3	1745.5
4	926.0
5	1163.5
6	1401.0
7	1229.5
8	1058.0
9	1037.0
10	1016.0
11	972.0
12	928.0
13	910.0
14	892.0
15	946.0
16	1000.0
17	1068.5
18	1137.0
19	1294.0
20	1451.0
21	1323.5
22	1196.0
23	1592.5
24	1989.0
25	2070.0
26	2406.5
27	2662.0
28	3225.5
29	3789.0
30	4079.0
31	4369.0
32	5017.0
33	5665.0
34	6174.5
35	6684.0
36	7854.0
37	9024.0
38	9291.5
39	9559.0
40	12461.5
41	15364.0
42	19426.0
43	23488.0
44	25922.5
45	28357.0
46	31842.0
47	35327.0
48	32512.5
49	29698.0
50	27407.5
51	25117.0
52	22854.0
53	20591.0
54	18633.5
55	16676.0
56	15146.0
57	13616.0
58	12877.5
59	12139.0
60	11525.5
61	10912.0
62	9659.0
63	8406.0
64	7795.5
65	7185.0
66	5934.0
67	4683.0
68	4191.5
69	3700.0
70	3060.5
71	2421.0
72	2158.5
73	1896.0
74	1699.0
75	1288.0
76	1074.0
77	869.5
78	665.0
79	530.5
80	396.0
81	277.0
82	158.0
83	122.5
84	87.0
85	67.5
86	48.0
87	34.0
88	20.0
89	39.0
90	58.0
91	32.0
92	6.0
93	4.0
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	327691.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.88016468584915
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.45267943568352	24.05443846398174
2	10.07657543289643	6.424857685402398
3	3.4328871068049023	3.283230189386055
4	1.5551913470565273	1.9831902504867869
5	0.9728678894358794	1.550759426639516
6	0.7463224612803265	1.4275729784619051
7	0.6016427412695694	1.3426328771603724
8	0.4669460499384537	1.1909053577155715
9	0.42803380497612575	1.2281209374374573
>10	5.902675795453067	43.17062855502935
>50	0.30032207807334727	6.020686976796033
>100	0.049887388384276955	2.9352757513669587
>500	0.009977477676855391	2.157243188748452
>1k	0.003990991070742156	3.2304573613873893
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4074	1.2432443979236536	No Hit
CTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGCT	3601	1.098901098901099	No Hit
AAACTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCT	1477	0.4507294982163074	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTT	1004	0.30638619919375265	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	964	0.2941795777119298	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCC	776	0.23680845674736262	No Hit
AATCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCT	769	0.23467229798804362	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGT	761	0.23223097369167905	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	698	0.2130055448578081	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	599	0.18279415669029664	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	587	0.1791321702457498	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCT	552	0.16845137644915487	No Hit
CCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC	551	0.16814621091210927	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	525	0.16021190694892443	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	391	0.119319724984818	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCT	370	0.11291124870686103	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.2972586979807197	0.0
2	0.0	0.0	0.0	1.7238801187704271	0.0
3	0.0	0.0	0.0	2.191393721524241	0.0
4	0.0	0.0	0.0	4.485323063495794	0.0
5	0.0	0.0	0.0	4.870747136784349	0.0
6	3.051655370455704E-4	0.0	0.0	6.662374004778893	0.0
7	3.051655370455704E-4	0.0	0.0	8.10031401533762	0.0
8	3.051655370455704E-4	0.0	0.0	8.731091180410814	0.0
9	3.051655370455704E-4	0.0	0.0	9.974945909408559	0.0
10	3.051655370455704E-4	0.0	0.0	11.403120622781827	0.0
11	3.051655370455704E-4	0.0	0.0	13.27897317900095	0.0
12	3.051655370455704E-4	0.0	0.0	14.208812570378802	0.0
13	3.051655370455704E-4	0.0	0.0	14.558837441370072	0.0
14	3.051655370455704E-4	0.0	0.0	14.983322703400459	0.0
15	3.051655370455704E-4	0.0	0.0	15.317784132002405	0.0
16	3.051655370455704E-4	0.0	0.0	16.00227043159562	0.0
17	3.051655370455704E-4	0.0	0.0	16.82469155393343	0.0
18	3.051655370455704E-4	0.0	0.0	17.815869218257443	0.0
19	3.051655370455704E-4	0.0	0.0	18.263547061103296	0.0
20	3.051655370455704E-4	0.0	0.0	18.74265695426484	0.0
21	3.051655370455704E-4	0.0	0.0	19.2586918774089	0.0
22	3.051655370455704E-4	0.0	0.0	19.82782560399889	0.0
23	3.051655370455704E-4	0.0	0.0	20.390245688773874	0.0
24	3.051655370455704E-4	0.0	0.0	20.773228437766065	0.0
25	3.051655370455704E-4	0.0	0.0	21.089685099682324	0.0
26	3.051655370455704E-4	0.0	0.0	21.392104146894482	0.0
27	3.051655370455704E-4	0.0	0.0	21.734499879459612	0.0
28	3.051655370455704E-4	0.0	0.0	22.071097466820877	0.0
29	3.051655370455704E-4	0.0	0.0	22.44767173953511	0.0
30	3.051655370455704E-4	0.0	0.0	22.85079541397231	0.0
31	3.051655370455704E-4	0.0	0.0	23.20478743694517	0.0
32	3.051655370455704E-4	0.0	0.0	23.5130046293612	0.0
33	3.051655370455704E-4	0.0	0.0	23.849907382259506	0.0
34	3.051655370455704E-4	0.0	0.0	24.18436881086145	0.0
35	3.051655370455704E-4	0.0	0.0	24.575591029353873	0.0
36	3.051655370455704E-4	0.0	0.0	24.89479418110354	0.0
37	6.103310740911408E-4	0.0	0.0	25.21308183624207	0.0
38	6.103310740911408E-4	0.0	0.0	25.55395174112197	0.0
39	6.103310740911408E-4	0.0	0.0	25.916793564669156	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTTA	20	7.025896E-4	45.000004	38
TAATACG	20	7.025896E-4	45.000004	1
GACAGCG	20	7.025896E-4	45.000004	1
ATTAACG	20	7.025896E-4	45.000004	1
TAGCCGT	40	6.7902874E-9	45.000004	44
AGAACCG	20	7.025896E-4	45.000004	1
CCGTAGT	40	6.7902874E-9	45.000004	27
CGTAGTG	40	6.7902874E-9	45.000004	28
AAGTTCG	20	7.025896E-4	45.000004	1
GCGGGTT	20	7.025896E-4	45.000004	5
ATGGTCG	20	7.025896E-4	45.000004	44
CATAAGA	20	7.025896E-4	45.000004	14
ATTTGCG	40	6.7902874E-9	45.000004	12
AGTACGG	40	6.7902874E-9	45.000004	2
TAGTCAT	20	7.025896E-4	45.000004	15
GTAGTGC	40	6.7902874E-9	45.000004	29
TGCGTAG	20	7.025896E-4	45.000004	1
CGAACAG	25	3.884681E-5	45.0	1
TGCCACG	25	3.884681E-5	45.0	1
GACCGAA	25	3.884681E-5	45.0	9
>>END_MODULE