FastQCFastQC Report
Sat 18 Jun 2016
SRR3552851_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552851_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences783418
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGCT49840.6361865568572589No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35730.45607836429594417No Hit
AAACTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCT15150.19338335345881766No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG12050.15381316232202988No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11880.15164318409839958No Hit
AAAAACTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTT11120.14194210498099355No Hit
CCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC8090.10326543428923No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7980.10186133073276334No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACCG253.8896636E-545.0000041
ACACGAT253.8896636E-545.00000428
TAGCCGT1100.045.00000444
TAGCACG302.164612E-645.0000041
TCGGACG253.8896636E-545.0000041
CGTAGTG253.8896636E-545.00000428
GCTAACG502.1827873E-1145.0000041
CAACGTT207.03191E-445.031
CGCATCG351.211265E-745.021
CGCTAAA207.03191E-445.035
GAACGAT207.03191E-445.043
GCGATAG406.8102963E-945.01
TATAGCG351.211265E-745.01
TAGGCCG207.03191E-445.01
TTACCGG700.045.02
TCGACGC207.03191E-445.019
CGCCCGA207.03191E-445.020
CTTACCG351.211265E-745.01
GCGACCG850.042.3529432
CTAACGG850.042.352942