##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552851_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	783418
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.297035554454965	31.0	31.0	34.0	30.0	34.0
2	31.578900408211197	31.0	31.0	34.0	30.0	34.0
3	31.728574017957207	31.0	31.0	34.0	30.0	34.0
4	35.64851969191415	37.0	35.0	37.0	35.0	37.0
5	35.105841326086455	37.0	35.0	37.0	33.0	37.0
6	35.2080741060328	37.0	35.0	37.0	33.0	37.0
7	35.308255618328914	37.0	35.0	37.0	33.0	37.0
8	35.702430886193575	37.0	35.0	37.0	35.0	37.0
9	37.22049531667641	39.0	37.0	39.0	34.0	39.0
10	36.5380358888869	38.0	35.0	39.0	32.0	39.0
11	36.72162753472603	39.0	35.0	39.0	32.0	39.0
12	36.54200311966281	39.0	35.0	39.0	32.0	39.0
13	36.307700359195216	39.0	35.0	39.0	32.0	39.0
14	37.43010627787465	39.0	36.0	40.0	32.0	41.0
15	37.348677206804034	39.0	36.0	40.0	32.0	41.0
16	37.257368352527	39.0	36.0	40.0	32.0	41.0
17	37.179971611579006	39.0	36.0	40.0	32.0	41.0
18	37.11535476591041	39.0	36.0	40.0	32.0	41.0
19	37.30046284359052	39.0	36.0	40.0	32.0	41.0
20	37.1211128669497	39.0	35.0	40.0	32.0	41.0
21	37.25350962066228	39.0	35.0	40.0	32.0	41.0
22	37.26448460464273	39.0	35.0	40.0	32.0	41.0
23	37.21118611009704	39.0	35.0	40.0	32.0	41.0
24	36.77720322994876	39.0	35.0	40.0	31.0	41.0
25	37.01291264688838	39.0	35.0	40.0	32.0	41.0
26	36.9679481451792	39.0	35.0	40.0	32.0	41.0
27	36.874414424994065	39.0	35.0	40.0	31.0	41.0
28	36.92715638394829	39.0	35.0	40.0	31.0	41.0
29	36.508849681779076	39.0	35.0	40.0	30.0	41.0
30	36.56048495183925	39.0	35.0	40.0	31.0	41.0
31	36.52777189188913	39.0	35.0	40.0	31.0	41.0
32	36.4827512260377	39.0	35.0	40.0	30.0	41.0
33	36.45466021970391	39.0	35.0	40.0	30.0	41.0
34	36.39640268668833	38.0	35.0	40.0	30.0	41.0
35	36.28998057231261	38.0	35.0	40.0	30.0	41.0
36	36.30246815876071	38.0	35.0	40.0	30.0	41.0
37	36.205647559795665	38.0	35.0	40.0	30.0	41.0
38	36.08919121082232	38.0	35.0	40.0	30.0	41.0
39	36.064532599455205	38.0	35.0	40.0	30.0	41.0
40	35.93229412650718	38.0	35.0	40.0	30.0	41.0
41	35.99334327268457	38.0	35.0	40.0	30.0	41.0
42	35.92123744922889	38.0	35.0	40.0	30.0	41.0
43	35.906466279814865	38.0	35.0	40.0	30.0	41.0
44	35.82292339466287	38.0	35.0	40.0	30.0	41.0
45	35.65079306321785	38.0	34.0	40.0	29.0	41.0
46	35.62431294660067	38.0	34.0	40.0	29.0	41.0
47	35.50460801258077	38.0	34.0	40.0	29.0	41.0
48	35.4820887444506	38.0	34.0	40.0	29.0	41.0
49	35.37771534480954	37.0	34.0	40.0	28.0	41.0
50	35.27366105961313	37.0	34.0	40.0	28.0	41.0
51	34.07267512362493	36.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	6.0
10	14.0
11	17.0
12	24.0
13	28.0
14	67.0
15	83.0
16	176.0
17	239.0
18	483.0
19	867.0
20	1355.0
21	1958.0
22	2782.0
23	3500.0
24	4128.0
25	4872.0
26	5779.0
27	6650.0
28	8421.0
29	11034.0
30	14166.0
31	19423.0
32	27325.0
33	38902.0
34	63591.0
35	70702.0
36	87880.0
37	128936.0
38	169308.0
39	110696.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.162115243714084	32.106870151056015	19.21949712669354	26.511517478536362
2	26.46096975050356	29.985780260346328	19.511040083327163	24.042209905822943
3	21.7314383892124	31.453196122631848	20.158459468636156	26.656906019519592
4	20.5759888080182	32.07074639592146	20.07702146236109	27.276243333699252
5	19.103084177284668	33.39634779900385	18.34882527590635	29.15174274780513
6	20.16114002997123	36.93826284307994	20.25202382380798	22.64857330314085
7	83.38588084521929	5.837624358899081	5.978290006101468	4.7982047897801685
8	85.11765111345412	4.998991598354901	5.240369764289307	4.642987523901672
9	78.88330367696427	8.26748938625357	8.359394346313206	4.489812590468945
10	36.113798763878286	34.643319402924114	13.427952893602138	15.814928939595466
11	28.484793558483467	28.130704170698145	25.25254717149721	18.13195509932118
12	29.062135411746986	24.82697614810995	27.578636181451028	18.53225225869204
13	25.886053167019398	28.048755581311635	26.0859464551491	19.979244796519865
14	20.389370680785994	29.90561871184987	28.891217715191637	20.813792892172504
15	18.40345766883069	30.385566836605744	30.40675603573061	20.80421945883296
16	22.36341263540026	28.02233290529449	28.607078213673926	21.007176245631324
17	20.908888996678655	27.254288259907227	28.26651927834183	23.570303465072286
18	22.655210883589604	27.70372904375442	26.855905787204275	22.7851542854517
19	24.59083145906783	30.4560273059848	25.549195959245257	19.403945275702117
20	25.453972208961247	29.545019389393655	25.264801166171825	19.736207235473273
21	23.936391555976503	28.9172574538752	26.958277701048484	20.18807328909982
22	23.159411706138997	26.486371260297826	26.532451386105503	23.821765647457678
23	22.196196666402866	29.172804301152134	25.658460745093937	22.972538287351068
24	20.282275873160945	29.33019154525426	28.419821857552417	21.96771072403238
25	21.687272950072632	28.507514506942655	26.82450492585057	22.980707617134147
26	23.001258587369705	30.46713248865867	24.683629939572487	21.847978984399134
27	20.61275079204205	29.193099979831967	26.340472136203154	23.853677091922833
28	21.665956105169908	29.145998687801406	28.895047088527452	20.292998118501234
29	21.389475350323835	28.307621218812944	28.63554322213684	21.66736020872638
30	23.548858974391703	27.884986048316478	27.580295576563213	20.9858594007286
31	25.034783474467016	27.5855290534555	25.447079336956772	21.932608135120716
32	24.256782458406622	27.475115455606076	26.948448976153216	21.319653109834086
33	23.98004641200483	27.05337380555463	26.438631739377954	22.52794804306258
34	20.46634108483594	27.638246759711926	28.481347122481232	23.414065032970903
35	21.541884409089402	27.928257967011227	29.314618760355266	21.215238863544112
36	23.844103658583286	27.661733582838284	27.383848724435744	21.110314034142693
37	22.83544672192878	30.09210919330421	25.15987633677041	21.9125677479966
38	23.01440610248935	28.712896563520367	26.871989155214713	21.40070817877557
39	22.072380261878077	26.08313824803617	26.8820731716657	24.962408318420053
40	22.825873288589232	27.343129721298208	26.190233055661217	23.64076393445134
41	21.214345343099087	25.013466629564295	28.883176031186412	24.889011996150202
42	23.624425274885184	27.045076829993693	26.406720294912805	22.923777600208318
43	22.46476338302158	26.13189893517892	27.422270103571783	23.981067578227716
44	22.857912378832246	25.372534202686175	27.176551981190116	24.59300143729146
45	23.848571260808406	26.415272562029468	27.357043111085016	22.379113066077114
46	21.32973712628507	25.947706077726068	29.920170330525973	22.80238646546288
47	21.833172074167305	27.37925347643276	26.80178397739138	23.98579047200856
48	21.93350165556574	25.45971626896497	30.407394264619906	22.19938781084938
49	22.605046092890387	24.952451947746923	29.305938847460745	23.136563111901946
50	21.4155150890074	25.50222231299255	27.662882394839027	25.41938020316102
51	21.124227423929497	25.49903116854604	30.111383705761163	23.265357701763296
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4205.0
1	3343.0
2	2481.0
3	1792.5
4	1104.0
5	1738.0
6	2372.0
7	2040.5
8	1709.0
9	1554.0
10	1399.0
11	1362.5
12	1326.0
13	1283.0
14	1240.0
15	1415.5
16	1591.0
17	1643.5
18	1696.0
19	1714.5
20	1733.0
21	1998.5
22	2264.0
23	2780.5
24	3297.0
25	4035.5
26	6072.0
27	7370.0
28	8753.5
29	10137.0
30	11425.0
31	12713.0
32	14255.0
33	15797.0
34	17790.0
35	19783.0
36	23482.5
37	27182.0
38	28510.5
39	29839.0
40	34969.0
41	40099.0
42	45565.0
43	51031.0
44	57762.5
45	64494.0
46	70144.5
47	75795.0
48	72847.0
49	69899.0
50	64823.5
51	59748.0
52	55643.5
53	51539.0
54	46896.0
55	42253.0
56	39113.5
57	35974.0
58	32907.0
59	29840.0
60	28829.0
61	27818.0
62	24946.0
63	22074.0
64	19341.5
65	16609.0
66	14555.5
67	12502.0
68	10401.5
69	8301.0
70	7908.5
71	7516.0
72	6248.5
73	4981.0
74	4396.0
75	2996.0
76	2181.0
77	1713.0
78	1245.0
79	1028.5
80	812.0
81	592.5
82	373.0
83	328.5
84	284.0
85	208.5
86	133.0
87	96.5
88	60.0
89	36.0
90	12.0
91	12.5
92	13.0
93	8.5
94	4.0
95	3.5
96	3.0
97	2.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	783418.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.418346915657153
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.52817435166361	20.982760331958087
2	9.60089441775905	5.264813076934277
3	3.3486627441788257	2.754443904702945
4	1.580005108950745	1.7328451282288877
5	0.9197694188794795	1.2609278504624977
6	0.6012758409732568	0.9891593759884955
7	0.46355618746435573	0.8896961053957974
8	0.38559339639856166	0.8457866808673815
9	0.34729376384267713	0.8569998808814547
>10	4.749257566616069	32.84830342003847
>50	1.3676097448469549	24.71445487426379
>100	0.10087001548557872	4.341231395505845
>500	0.004222465764512597	0.7720774812085479
>1k	0.0028149771763417318	1.7465004935635546
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGCT	4984	0.6361865568572589	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3573	0.45607836429594417	No Hit
AAACTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCT	1515	0.19338335345881766	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1205	0.15381316232202988	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1188	0.15164318409839958	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTT	1112	0.14194210498099355	No Hit
CCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC	809	0.10326543428923	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	798	0.10186133073276334	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.276457778606057E-4	0.0	0.0	0.7363884924778343	0.0
2	1.276457778606057E-4	0.0	0.0	0.9927012144219306	0.0
3	1.276457778606057E-4	0.0	0.0	1.286797086612766	0.0
4	1.276457778606057E-4	0.0	0.0	2.6328218141528534	0.0
5	1.276457778606057E-4	0.0	0.0	2.875731729421586	0.0
6	1.276457778606057E-4	0.0	0.0	3.982803560806619	0.0
7	1.276457778606057E-4	0.0	0.0	4.798332435558029	0.0
8	1.276457778606057E-4	0.0	0.0	5.187524412255016	0.0
9	1.276457778606057E-4	0.0	0.0	5.919955885619172	0.0
10	1.276457778606057E-4	0.0	0.0	6.894403753807035	0.0
11	1.276457778606057E-4	0.0	0.0	8.100018125700457	0.0
12	1.276457778606057E-4	0.0	0.0	8.665105984289358	0.0
13	1.276457778606057E-4	0.0	0.0	8.896527779550636	0.0
14	1.276457778606057E-4	0.0	0.0	9.164328621502186	0.0
15	1.276457778606057E-4	0.0	0.0	9.401494476767192	0.0
16	1.276457778606057E-4	0.0	0.0	9.874932666852178	0.0
17	1.276457778606057E-4	0.0	0.0	10.478697196132844	0.0
18	1.276457778606057E-4	0.0	0.0	11.135307077447798	0.0
19	1.276457778606057E-4	0.0	0.0	11.469100786553284	0.0
20	1.276457778606057E-4	0.0	0.0	11.841826457906253	0.0
21	1.276457778606057E-4	0.0	0.0	12.268673939072118	0.0
22	1.276457778606057E-4	0.0	0.0	12.734708674041189	0.0
23	1.276457778606057E-4	0.0	0.0	13.189382934780665	0.0
24	1.276457778606057E-4	0.0	0.0	13.519474916328193	0.0
25	1.276457778606057E-4	0.0	0.0	13.813060205407586	0.0
26	1.276457778606057E-4	0.0	0.0	14.076265799356154	0.0
27	1.276457778606057E-4	0.0	0.0	14.349044826644269	0.0
28	1.276457778606057E-4	0.0	0.0	14.672754519298765	0.0
29	1.276457778606057E-4	0.0	0.0	14.976551470607006	0.0
30	0.004978185336563623	0.0	0.0	15.350553599738582	0.0
31	0.004978185336563623	0.0	0.0	15.640054223926436	0.0
32	0.004978185336563623	0.0	0.0	15.947042319681191	0.0
33	0.004978185336563623	0.0	0.0	16.264114431886938	0.0
34	0.005105831114424228	0.0	0.0	16.559231470300656	0.0
35	0.005105831114424228	0.0	0.0	16.89698219851982	0.0
36	0.005105831114424228	0.0	0.0	17.191205716488515	0.0
37	0.005105831114424228	0.0	0.0	17.49142858601666	0.0
38	0.005105831114424228	0.0	0.0	17.79675728665923	0.0
39	0.005105831114424228	0.0	0.0	18.116637605977907	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACCG	25	3.8896636E-5	45.000004	1
ACACGAT	25	3.8896636E-5	45.000004	28
TAGCCGT	110	0.0	45.000004	44
TAGCACG	30	2.164612E-6	45.000004	1
TCGGACG	25	3.8896636E-5	45.000004	1
CGTAGTG	25	3.8896636E-5	45.000004	28
GCTAACG	50	2.1827873E-11	45.000004	1
CAACGTT	20	7.03191E-4	45.0	31
CGCATCG	35	1.211265E-7	45.0	21
CGCTAAA	20	7.03191E-4	45.0	35
GAACGAT	20	7.03191E-4	45.0	43
GCGATAG	40	6.8102963E-9	45.0	1
TATAGCG	35	1.211265E-7	45.0	1
TAGGCCG	20	7.03191E-4	45.0	1
TTACCGG	70	0.0	45.0	2
TCGACGC	20	7.03191E-4	45.0	19
CGCCCGA	20	7.03191E-4	45.0	20
CTTACCG	35	1.211265E-7	45.0	1
GCGACCG	85	0.0	42.35294	32
CTAACGG	85	0.0	42.35294	2
>>END_MODULE