SRR3552847_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3552847_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	564857
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT	7440	1.3171475258339722	Illumina Single End Adapter 2 (95% over 22bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCC	5500	0.9736977677536084	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGT	5200	0.9205869804215934	No Hit
AATCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCT	4913	0.8697776605406324	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3933	0.6962824219227167	No Hit
AAACTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCT	1915	0.3390238591360291	No Hit
AATGACTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTT	1301	0.23032378106317172	No Hit
CCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	1279	0.2264289899921573	Illumina Single End Adapter 2 (95% over 21bp)
AAAAACTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTT	1235	0.21863940785012845	No Hit
AACTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG	1105	0.1956247333395886	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCT	1036	0.18340925225322516	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	976	0.17278709478682214	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	910	0.16110272157377883	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGATTATGCTCGTAT	796	0.14092062238761316	No Hit
ACCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG	776	0.13737990323214547	No Hit
AAAAAAGGGCATCTTCTATGAAAAAACTTTCTACCCCTAACACTAGCATTA	717	0.12693478172351585	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGATTATGCTC	695	0.12303999065250142	No Hit
AACCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCT	675	0.11949927149703377	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	663	0.11737484000375316	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCT	625	0.11064747360836459	No Hit
AGCCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCT	619	0.1095852578617243	No Hit
GCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	586	0.10374307125520264	Illumina Single End Adapter 2 (95% over 21bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGATCGA	45	3.8380676E-10	45.000004	8
TCGACGT	45	3.8380676E-10	45.000004	26
GACAACG	20	7.0302375E-4	45.0	1
TAAACCG	35	1.2104647E-7	45.0	1
ATTGTCG	20	7.0302375E-4	45.0	1
TTCGTAC	35	1.2104647E-7	45.0	33
TAACGCG	20	7.0302375E-4	45.0	1
TAGCCGT	35	1.2104647E-7	45.0	44
GCCGATG	25	3.8882783E-5	45.0	9
TACGCCG	20	7.0302375E-4	45.0	1
CGCTCAG	35	1.2104647E-7	45.0	1
CGTAGCG	20	7.0302375E-4	45.0	1
CGTAGCA	20	7.0302375E-4	45.0	11
TGTTCGA	25	3.8882783E-5	45.0	42
AATTCGT	35	1.2104647E-7	45.0	31
CGTACAG	20	7.0302375E-4	45.0	1
TCGTACA	35	1.2104647E-7	45.0	34
CAATACG	20	7.0302375E-4	45.0	1
TACGTAG	25	3.8882783E-5	45.0	1
AGTCGCA	20	7.0302375E-4	45.0	11

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content