##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552847_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	564857
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.964939798922558	31.0	31.0	33.0	28.0	34.0
2	31.252844525251525	31.0	31.0	34.0	28.0	34.0
3	31.461637193130297	31.0	31.0	34.0	30.0	34.0
4	35.424845580385835	37.0	35.0	37.0	33.0	37.0
5	34.73717241708964	35.0	35.0	37.0	32.0	37.0
6	34.85725590724732	35.0	35.0	37.0	32.0	37.0
7	34.914431440169814	35.0	35.0	37.0	32.0	37.0
8	35.315625016597124	37.0	35.0	37.0	33.0	37.0
9	36.83331002359889	39.0	37.0	39.0	33.0	39.0
10	36.103734219457316	37.0	35.0	39.0	31.0	39.0
11	36.37372821793834	38.0	35.0	39.0	32.0	39.0
12	36.18423069909729	37.0	35.0	39.0	32.0	39.0
13	35.93673265977053	37.0	35.0	39.0	31.0	39.0
14	36.96474505936901	39.0	36.0	40.0	32.0	41.0
15	36.91775440509722	38.0	35.0	40.0	32.0	41.0
16	36.76989397316489	38.0	35.0	40.0	31.0	41.0
17	36.7159546575505	38.0	35.0	40.0	31.0	41.0
18	36.634202992969904	38.0	35.0	40.0	31.0	41.0
19	36.71254140428462	38.0	35.0	40.0	31.0	41.0
20	36.639090955764026	38.0	35.0	40.0	31.0	41.0
21	36.76186185176071	38.0	35.0	40.0	31.0	41.0
22	36.75496276048628	39.0	35.0	40.0	31.0	41.0
23	36.7141488907812	39.0	35.0	40.0	31.0	41.0
24	36.19496615957667	38.0	35.0	40.0	30.0	41.0
25	36.48539718902306	38.0	35.0	40.0	31.0	41.0
26	36.286412313204934	38.0	35.0	40.0	30.0	41.0
27	36.267974018202835	38.0	35.0	40.0	30.0	41.0
28	36.371219618416696	38.0	35.0	40.0	30.0	41.0
29	35.75611880529054	38.0	34.0	40.0	29.0	41.0
30	36.034314879695216	38.0	35.0	40.0	30.0	41.0
31	35.88724225777498	38.0	34.0	40.0	29.0	41.0
32	35.79820556353201	38.0	34.0	40.0	29.0	41.0
33	35.96382447238859	38.0	35.0	40.0	30.0	41.0
34	35.774146376870604	38.0	35.0	40.0	29.0	41.0
35	35.78814992113048	38.0	35.0	40.0	29.0	41.0
36	35.73739548239643	38.0	35.0	40.0	29.0	41.0
37	35.66974119113333	38.0	34.0	40.0	29.0	41.0
38	35.65632894697242	38.0	34.0	40.0	29.0	41.0
39	35.554150873583936	38.0	34.0	40.0	28.0	41.0
40	35.43747355525381	38.0	34.0	40.0	28.0	41.0
41	35.594507990517954	38.0	34.0	40.0	29.0	41.0
42	35.49229450993791	38.0	34.0	40.0	28.0	41.0
43	35.48274696073519	38.0	34.0	40.0	28.0	41.0
44	35.42081978270607	38.0	34.0	40.0	28.0	41.0
45	35.208155338430785	38.0	34.0	40.0	27.0	41.0
46	35.14988041221052	38.0	34.0	40.0	27.0	41.0
47	35.056155805805716	37.0	34.0	40.0	27.0	41.0
48	35.12673827181039	37.0	34.0	40.0	27.0	41.0
49	35.02780526752789	37.0	34.0	40.0	27.0	41.0
50	34.954059168957805	37.0	34.0	40.0	27.0	41.0
51	33.72577838284734	36.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	10.0
11	12.0
12	15.0
13	23.0
14	45.0
15	56.0
16	118.0
17	202.0
18	396.0
19	766.0
20	1251.0
21	1776.0
22	2520.0
23	3104.0
24	3468.0
25	4152.0
26	4842.0
27	5754.0
28	7361.0
29	9683.0
30	12648.0
31	17130.0
32	23416.0
33	32944.0
34	49591.0
35	58626.0
36	71131.0
37	93999.0
38	104852.0
39	54956.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.70845187366006	35.88695900024254	19.3008141883698	26.103774937727604
2	21.10551874191167	35.361339241613365	19.869807756653454	23.66333425982151
3	20.386752753351733	31.06591579815776	23.723703521422234	24.823627927068266
4	21.917405644260405	31.194089831585693	20.179620682756873	26.708883841397025
5	16.749726036855346	36.36495608623068	19.513434373655635	27.37188350325835
6	20.35878107202354	35.141637618016595	22.24828584933886	22.251295460621005
7	75.40864324953041	9.185864740987542	9.075217267379179	6.330274742102869
8	77.49200240060759	5.633815284222378	6.973269340735796	9.900912974434236
9	72.73398399949014	8.902430172592355	12.207691504221422	6.155894323696086
10	33.32365536764172	34.54626569202471	14.449851909421321	17.68022703091225
11	24.2587061858134	25.174336159417336	30.945885418787412	19.621072235981853
12	25.323754507778073	23.352636153929225	33.17264369566102	18.150965642631675
13	22.40071380898174	29.89889476451562	26.606734093761787	21.093657332740854
14	16.61712610448308	30.33475729255369	33.699148634079066	19.34896796888416
15	15.42195635355497	31.324565332464676	29.55402871877307	23.699449595207284
16	16.483995064237497	28.397098734724008	31.445303855666122	23.67360234537237
17	15.82542130132051	29.02345195332624	30.204812899548028	24.94631384580522
18	18.276838208608552	28.009212951242528	27.498641249024093	26.21530759112483
19	18.91788541170597	32.30162678341598	29.398414111890265	19.382073692987785
20	22.18933287540032	30.68688181256495	26.76783681533556	20.355948496699163
21	18.421653622067176	31.170189977286288	27.060300217577193	23.347856183069343
22	17.8740814046741	29.29502511255061	28.253522572969796	24.57737090980549
23	20.762600091704623	29.322111614089934	23.833288779283958	26.081999514921478
24	16.459564102064768	33.05101999267071	27.341433318521325	23.147982586743193
25	20.050915541455623	28.378332923200034	26.469708262445184	25.101043272899158
26	21.627774817343152	33.33923453192578	23.25508934119609	21.77790130953498
27	16.7686688843371	29.718141051628997	28.5242105524053	24.988979511628607
28	22.394163478544126	27.66204543804892	28.81525766698474	21.12853341642221
29	18.46308003618615	33.64656895462037	26.58779124628003	21.302559762913447
30	21.44861442807649	27.059238001830554	29.7946205853871	21.697526984705863
31	24.492216614116494	30.479395670054544	24.617026964346728	20.41136075148223
32	20.886879334061543	30.02228882708367	25.1539770242734	23.93685481458139
33	22.95678375234794	25.294012466872147	29.743634229548366	22.005569551231552
34	21.04532651626872	29.212526356228214	25.509111155566806	24.23303597193626
35	20.59477000373546	26.64798347192298	28.77383125286577	23.98341527147579
36	22.111614089937806	27.44305195828325	31.930028308049646	18.5153056437293
37	18.579569696401034	29.69902116818947	27.898211405718616	23.82319772969088
38	20.3796713150408	27.67231352359978	31.62871310791935	20.319302053440076
39	20.883692686821618	27.354711015354326	29.345126288600476	22.416470009223573
40	23.797527515813737	26.32453877707101	26.12023928180053	23.757694425314728
41	22.073374323058754	22.376636918724564	31.01404426253016	24.53594449568652
42	24.385463931579142	25.03058296170535	24.870719491835988	25.713233614879517
43	20.066140633824137	24.928610250027884	29.773907378327614	25.23134173782037
44	21.489509734322137	23.6454536280864	28.269986917042722	26.59504972054874
45	24.403875671187574	26.608858525255062	27.3775486539071	21.609717149650265
46	18.8984114563509	25.556202720334525	33.239917359614914	22.30546846369966
47	21.147653299861734	25.79608644311746	27.48040654537343	25.575853711647373
48	21.439054486356724	22.585008241023836	34.65815241733748	21.317784855281957
49	21.77259023080178	21.747274088840186	31.415030706886878	25.065104973471165
50	19.620718164066304	22.767178241572648	27.96176731455926	29.65033627980179
51	18.96019700561381	22.08258019286297	34.67815748056588	24.27906532095734
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4529.0
1	3587.0
2	2645.0
3	1792.5
4	940.0
5	1340.5
6	1741.0
7	1505.5
8	1270.0
9	1224.0
10	1178.0
11	1235.0
12	1292.0
13	1242.5
14	1193.0
15	1363.5
16	1534.0
17	1541.0
18	1548.0
19	1603.5
20	1659.0
21	1568.0
22	1477.0
23	1962.0
24	2447.0
25	2866.5
26	3728.0
27	4170.0
28	4958.5
29	5747.0
30	6923.5
31	8100.0
32	9392.0
33	10684.0
34	12074.5
35	13465.0
36	15208.0
37	16951.0
38	18435.5
39	19920.0
40	24780.5
41	29641.0
42	41577.0
43	53513.0
44	58689.5
45	63866.0
46	67781.0
47	71696.0
48	65119.0
49	58542.0
50	51742.0
51	44942.0
52	39219.5
53	33497.0
54	28986.5
55	24476.0
56	21467.5
57	18459.0
58	16747.5
59	15036.0
60	13765.0
61	12494.0
62	11077.5
63	9661.0
64	8352.0
65	7043.0
66	6014.5
67	4986.0
68	4138.0
69	3290.0
70	2894.5
71	2499.0
72	2335.5
73	2172.0
74	1602.0
75	1023.0
76	1014.0
77	824.5
78	635.0
79	410.5
80	186.0
81	177.5
82	169.0
83	123.5
84	78.0
85	98.0
86	118.0
87	69.0
88	20.0
89	13.5
90	7.0
91	6.5
92	6.0
93	4.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	564857.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.09223562957365
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.40702061267555	26.812931710937015
2	10.166039240704775	7.134980889086079
3	3.7085805678444177	3.90427149414163
4	1.8510459140537234	2.598293575085312
5	1.1120103466910485	1.9511464554303082
6	0.7553594700733869	1.5904351505307106
7	0.5766022048583247	1.4163982306194016
8	0.497284101838003	1.396064870123208
9	0.3704655340376853	1.1700417431777659
>10	4.095527334814711	31.59998472618961
>50	0.3899111643505296	8.550089286451408
>100	0.056936180452739814	3.842259703128915
>500	0.007625381310634796	1.8148067582844485
>1k	0.004066870032338557	2.982220050942802
>5k	0.0015250762621269592	3.236075355871414
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT	7440	1.3171475258339722	Illumina Single End Adapter 2 (95% over 22bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCC	5500	0.9736977677536084	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGT	5200	0.9205869804215934	No Hit
AATCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCT	4913	0.8697776605406324	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3933	0.6962824219227167	No Hit
AAACTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCT	1915	0.3390238591360291	No Hit
AATGACTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTT	1301	0.23032378106317172	No Hit
CCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	1279	0.2264289899921573	Illumina Single End Adapter 2 (95% over 21bp)
AAAAACTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTT	1235	0.21863940785012845	No Hit
AACTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG	1105	0.1956247333395886	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCT	1036	0.18340925225322516	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	976	0.17278709478682214	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	910	0.16110272157377883	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGATTATGCTCGTAT	796	0.14092062238761316	No Hit
ACCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG	776	0.13737990323214547	No Hit
AAAAAAGGGCATCTTCTATGAAAAAACTTTCTACCCCTAACACTAGCATTA	717	0.12693478172351585	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGATTATGCTC	695	0.12303999065250142	No Hit
AACCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCT	675	0.11949927149703377	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	663	0.11737484000375316	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCT	625	0.11064747360836459	No Hit
AGCCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCT	619	0.1095852578617243	No Hit
GCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	586	0.10374307125520264	Illumina Single End Adapter 2 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7703595777338336E-4	0.0	0.0	1.5570312486169067	0.0
2	1.7703595777338336E-4	0.0	0.0	2.116110803265251	0.0
3	1.7703595777338336E-4	0.0	0.0	2.9294139932761745	0.0
4	1.7703595777338336E-4	0.0	0.0	6.543426035262022	0.0
5	1.7703595777338336E-4	0.0	0.0	7.015226862728088	0.0
6	1.7703595777338336E-4	0.0	0.0	9.29881368204696	0.0
7	1.7703595777338336E-4	0.0	0.0	10.999775164333627	0.0
8	1.7703595777338336E-4	0.0	0.0	11.852734408885789	0.0
9	1.7703595777338336E-4	0.0	0.0	14.284146252945435	0.0
10	1.7703595777338336E-4	0.0	0.0	16.3496247722875	0.0
11	1.7703595777338336E-4	0.0	0.0	19.647450593690085	0.0
12	1.7703595777338336E-4	0.0	0.0	20.83235225906734	0.0
13	1.7703595777338336E-4	0.0	0.0	21.296894612264698	0.0
14	1.7703595777338336E-4	0.0	0.0	21.907845702540644	0.0
15	1.7703595777338336E-4	0.0	0.0	22.33786604397219	0.0
16	1.7703595777338336E-4	0.0	0.0	23.140193004601166	0.0
17	1.7703595777338336E-4	0.0	0.0	24.20895908167908	0.0
18	1.7703595777338336E-4	0.0	0.0	25.593380271466938	0.0
19	1.7703595777338336E-4	0.0	0.0	26.267179126752435	0.0
20	1.7703595777338336E-4	0.0	0.0	26.836349730993863	0.0
21	1.7703595777338336E-4	0.0	0.0	27.544316526129624	0.0
22	1.7703595777338336E-4	0.0	0.0	28.271403204704907	0.0
23	1.7703595777338336E-4	0.0	0.0	29.002915782224527	0.0
24	1.7703595777338336E-4	0.0	0.0	29.549602819828735	0.0
25	1.7703595777338336E-4	0.0	0.0	30.00033636831977	0.0
26	1.7703595777338336E-4	0.0	0.0	30.412299042058432	0.0
27	1.7703595777338336E-4	0.0	0.0	30.84745342626541	0.0
28	1.7703595777338336E-4	0.0	0.0	31.281722630683518	0.0
29	1.7703595777338336E-4	0.0	0.0	31.760958968376066	0.0
30	1.7703595777338336E-4	0.0	0.0	32.34765613243706	0.0
31	1.7703595777338336E-4	0.0	0.0	32.80281557987243	0.0
32	1.7703595777338336E-4	0.0	0.0	33.238678107910495	0.0
33	1.7703595777338336E-4	0.0	0.0	33.669406593173136	0.0
34	1.7703595777338336E-4	0.0	0.0	34.08933588501161	0.0
35	1.7703595777338336E-4	0.0	0.0	34.55918931694217	0.0
36	1.7703595777338336E-4	0.0	0.0	34.972922350258564	0.0
37	1.7703595777338336E-4	0.0	0.0	35.40666044680335	0.0
38	1.7703595777338336E-4	0.0	0.0	35.83491042865717	0.0
39	1.7703595777338336E-4	0.0	0.0	36.26687816562422	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGATCGA	45	3.8380676E-10	45.000004	8
TCGACGT	45	3.8380676E-10	45.000004	26
GACAACG	20	7.0302375E-4	45.0	1
TAAACCG	35	1.2104647E-7	45.0	1
ATTGTCG	20	7.0302375E-4	45.0	1
TTCGTAC	35	1.2104647E-7	45.0	33
TAACGCG	20	7.0302375E-4	45.0	1
TAGCCGT	35	1.2104647E-7	45.0	44
GCCGATG	25	3.8882783E-5	45.0	9
TACGCCG	20	7.0302375E-4	45.0	1
CGCTCAG	35	1.2104647E-7	45.0	1
CGTAGCG	20	7.0302375E-4	45.0	1
CGTAGCA	20	7.0302375E-4	45.0	11
TGTTCGA	25	3.8882783E-5	45.0	42
AATTCGT	35	1.2104647E-7	45.0	31
CGTACAG	20	7.0302375E-4	45.0	1
TCGTACA	35	1.2104647E-7	45.0	34
CAATACG	20	7.0302375E-4	45.0	1
TACGTAG	25	3.8882783E-5	45.0	1
AGTCGCA	20	7.0302375E-4	45.0	11
>>END_MODULE