##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552845_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	316896
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.054216525295367	31.0	31.0	33.0	28.0	34.0
2	31.33178392911239	31.0	31.0	34.0	30.0	34.0
3	31.535232379077048	31.0	31.0	34.0	30.0	34.0
4	35.49625113601939	37.0	35.0	37.0	33.0	37.0
5	34.83368360597799	35.0	35.0	37.0	32.0	37.0
6	34.90838319196203	35.0	35.0	37.0	32.0	37.0
7	34.858388872058974	35.0	35.0	37.0	32.0	37.0
8	35.27156859032616	37.0	35.0	37.0	33.0	37.0
9	36.77252473997778	39.0	37.0	39.0	32.0	39.0
10	36.199674341108754	38.0	35.0	39.0	32.0	39.0
11	36.38461829748561	38.0	35.0	39.0	32.0	39.0
12	36.066797940018176	37.0	35.0	39.0	32.0	39.0
13	35.651365116631325	37.0	35.0	39.0	30.0	39.0
14	36.66291780268605	38.0	35.0	40.0	31.0	41.0
15	36.666568842774915	38.0	35.0	40.0	31.0	41.0
16	36.596880364536	38.0	35.0	40.0	31.0	41.0
17	36.49628900333232	38.0	35.0	40.0	31.0	41.0
18	36.386114056346564	38.0	35.0	40.0	31.0	41.0
19	36.442867691608605	38.0	35.0	40.0	31.0	41.0
20	36.32448184893467	38.0	35.0	40.0	30.0	41.0
21	36.39500025244875	38.0	35.0	40.0	31.0	41.0
22	36.37327072604261	38.0	35.0	40.0	30.0	41.0
23	36.29993436332425	38.0	35.0	40.0	31.0	41.0
24	35.85128243966475	38.0	34.0	40.0	29.0	41.0
25	36.07660872967787	38.0	34.0	40.0	30.0	41.0
26	35.93087953145511	38.0	34.0	40.0	30.0	41.0
27	35.87883406543472	38.0	34.0	40.0	30.0	41.0
28	35.91649941936787	38.0	34.0	40.0	30.0	41.0
29	35.3636240280723	38.0	34.0	40.0	27.0	41.0
30	35.4714606684843	38.0	34.0	40.0	28.0	41.0
31	35.29389452691104	38.0	34.0	40.0	27.0	41.0
32	35.33090035847723	38.0	34.0	40.0	27.0	41.0
33	35.26848240432192	38.0	34.0	40.0	27.0	41.0
34	35.11255427648187	38.0	34.0	40.0	27.0	41.0
35	35.12202741593457	38.0	34.0	40.0	27.0	41.0
36	35.042720640210035	37.0	34.0	40.0	26.0	41.0
37	34.91263064222963	37.0	33.0	40.0	26.0	41.0
38	34.865832954660206	37.0	33.0	40.0	26.0	41.0
39	34.78622324043219	37.0	33.0	40.0	25.0	41.0
40	34.57738500959305	37.0	33.0	40.0	24.0	41.0
41	34.626057129152784	37.0	33.0	40.0	25.0	41.0
42	34.60644817227103	37.0	33.0	40.0	25.0	41.0
43	34.5505086842371	37.0	33.0	40.0	25.0	41.0
44	34.46692921336969	37.0	33.0	40.0	24.0	41.0
45	34.29240507926891	37.0	33.0	40.0	24.0	41.0
46	34.247888897303845	36.0	33.0	40.0	23.0	41.0
47	34.09038612036757	36.0	33.0	40.0	23.0	41.0
48	34.10684262344744	36.0	33.0	40.0	23.0	41.0
49	33.99643100575583	36.0	33.0	39.0	24.0	41.0
50	33.90084128546905	36.0	33.0	39.0	23.0	41.0
51	32.6933978339897	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	2.0
10	10.0
11	10.0
12	7.0
13	14.0
14	21.0
15	45.0
16	97.0
17	182.0
18	351.0
19	649.0
20	1076.0
21	1554.0
22	2014.0
23	2526.0
24	2916.0
25	3211.0
26	3558.0
27	4122.0
28	5125.0
29	6267.0
30	8073.0
31	10674.0
32	14391.0
33	20098.0
34	31705.0
35	33215.0
36	35536.0
37	47343.0
38	54016.0
39	28084.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.660708876098152	35.33146521256185	19.30980510956276	25.69802080177724
2	22.125870948197516	34.61861304655155	19.839000807836012	23.416515197414924
3	20.747816318287388	34.530255983035445	19.371023932141775	25.350903766535392
4	19.092383621124913	34.169569827325056	19.40005553872564	27.337991012824396
5	16.429680399878823	36.27246793900838	19.39942441684338	27.898427244269413
6	20.135312531556092	37.87204634959103	20.661037059476925	21.331604059375948
7	73.29533979602141	10.741063314147228	8.868524689488035	7.09507220034333
8	75.44620317075633	9.283487327072603	7.4450292840553365	7.825280218115722
9	69.82227607795618	12.025711905483186	12.576681308694335	5.575330707866303
10	36.61958497425023	31.28849843481773	13.754039180046451	18.33787741088559
11	29.29794001817631	28.005717964253257	25.019564778349995	17.67677723922044
12	29.10260779561749	25.260337776431385	28.476850449358782	17.160203978592346
13	24.16376350600828	33.15788145006564	22.84156316267798	19.836791881248107
14	16.036491467232153	34.80100727052408	29.389452691103706	19.77304857114006
15	14.594693527213975	32.635312531556096	31.6412955669999	21.12869837423003
16	16.95792941532869	29.48222760779562	30.488867009996973	23.070975966878724
17	16.07656770675553	29.40018176310209	27.74033121276381	26.78291931737857
18	19.447074118953854	28.00761132990003	26.254670301928705	26.290644249217408
19	21.58594617792588	33.56874179541553	25.421904978289405	19.42340704836918
20	23.79613500959305	29.95683126325356	25.082361405634657	21.164672321518733
21	19.964278501464204	31.85114359285065	25.174505200444308	23.010072705240837
22	18.649336059779863	30.333611026961528	25.33039230536201	25.686660607896595
23	21.035923457538118	30.81705038877108	21.960832575987073	26.186193577703726
24	17.342913763506008	31.103264162375037	26.804061900434213	24.74976017368474
25	20.56983994749066	29.286579824295668	25.069738967989498	25.07384126022417
26	20.267848126830255	33.02976370796728	24.496680298899324	22.20570786630314
27	17.18923558517621	30.572806220337274	25.002208926587905	27.23574926789862
28	21.312039281025953	28.250593254569324	29.29541553064728	21.141951933757447
29	21.218002120569523	29.34369635464001	27.98362869837423	21.45467282641624
30	22.860181258204584	27.603062203372712	24.9062784004847	24.630478137938
31	24.303872563869536	29.604980813894777	25.05680096940321	21.034345652832474
32	26.300111077451277	27.058719579925278	25.091512672927397	21.549656669696052
33	22.171311723720084	26.96657578511562	28.387862263960418	22.474250227203875
34	21.040972432596185	29.668724124002825	25.859903564576392	23.4303998788246
35	22.70871200646269	24.916691911541957	31.023427244269413	21.351168837725943
36	23.151444006866605	26.912299303241443	30.68419923255579	19.25205745733616
37	20.246705543774613	29.3992350802787	27.330101989296175	23.02395738665051
38	22.255250934060385	26.884214379480966	30.36895385236797	20.49158083409068
39	23.459431485408462	25.717585580127235	29.320975966878727	21.50200696758558
40	23.633305563970513	26.020839644552158	26.483767545188325	23.862087246289004
41	19.311382914268403	24.420630112087245	31.204874785418564	25.063112188225787
42	25.813516106230434	24.592295264061395	26.843822579016457	22.75036605069171
43	20.764225487226092	23.903425729576895	29.994067454306776	25.338281328890233
44	22.33193224275472	23.35718974048268	26.238576693931133	28.072301322831468
45	23.656341512672928	26.16094870241341	28.07324800565485	22.109461779258808
46	21.257447238210645	24.556636877713824	31.619206301120872	22.566709582954662
47	20.742136221347067	25.889881853983642	27.326315258002627	26.041666666666668
48	20.82449762698172	23.922043825103504	33.01114561244067	22.2423129354741
49	21.623497929920227	21.779700595779055	31.709141169342626	24.887660304958093
50	19.852254367363425	23.621945370089872	27.496718166212258	29.029082096334445
51	19.71530091891346	23.458484802585076	32.733451984247196	24.092762294254268
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5499.0
1	4317.5
2	3136.0
3	2036.0
4	936.0
5	997.5
6	1059.0
7	979.5
8	900.0
9	890.0
10	880.0
11	885.0
12	890.0
13	903.5
14	917.0
15	951.5
16	986.0
17	976.5
18	967.0
19	1034.0
20	1101.0
21	1083.0
22	1065.0
23	1060.5
24	1056.0
25	1242.5
26	1807.0
27	2185.0
28	2670.5
29	3156.0
30	3370.5
31	3585.0
32	4119.0
33	4653.0
34	5072.5
35	5492.0
36	6037.0
37	6582.0
38	7299.5
39	8017.0
40	10968.5
41	13920.0
42	18943.5
43	23967.0
44	27662.5
45	31358.0
46	35015.0
47	38672.0
48	35093.5
49	31515.0
50	29129.0
51	26743.0
52	23298.0
53	19853.0
54	17591.0
55	15329.0
56	14126.5
57	12924.0
58	11980.0
59	11036.0
60	10386.5
61	9737.0
62	9024.0
63	8311.0
64	7331.5
65	6352.0
66	5063.0
67	3774.0
68	3382.5
69	2991.0
70	2469.0
71	1947.0
72	1733.5
73	1520.0
74	1227.0
75	776.5
76	619.0
77	494.0
78	369.0
79	339.5
80	310.0
81	216.0
82	122.0
83	91.0
84	60.0
85	35.5
86	11.0
87	8.0
88	5.0
89	7.0
90	9.0
91	7.5
92	6.0
93	5.0
94	4.0
95	3.0
96	2.0
97	3.0
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	316896.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.11637370595993
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.60071503340119	25.367379051898563
2	10.652746701181634	7.055606815025256
3	3.49495711728615	3.472209179470578
4	1.6756176099028988	2.2196151583132706
5	0.9396999100785788	1.5559726696809577
6	0.6942560589786688	1.379474585805873
7	0.4698153216511009	1.0891005835208536
8	0.3979958465736808	1.0544143350843125
9	0.31420717961189787	0.9364862143010956
>10	4.08668814771454	32.808727963516375
>50	0.5905099786412156	12.325281235310563
>100	0.07181878118609378	4.440298841258699
>500	0.005984898432174481	1.641410872058806
>1k	0.0039899322881163215	2.980899921404441
>5k	9.974830720290804E-4	1.6731225733503428
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCT	5065	1.598316166818136	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4930	1.5557154397657276	No Hit
AAACTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCT	1866	0.5888367161466223	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1175	0.3707841058265172	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTT	1053	0.3322856710087852	No Hit
AATCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCT	980	0.3092497223063718	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	901	0.28432040795718466	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGT	858	0.2707512874886398	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCC	856	0.2701201656063819	No Hit
CCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC	762	0.24045743714026052	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCT	612	0.1931232959709179	No Hit
ACCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG	432	0.13632232656770676	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	402	0.12685549833383825	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCT	361	0.11391749974755125	No Hit
GCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC	355	0.11202413410077755	No Hit
AACTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG	333	0.10508179339594062	No Hit
AGCCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCT	330	0.10413511057255377	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	325	0.102557305866909	No Hit
GAACTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCT	322	0.10161062304352216	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.311218822579016E-4	0.0	0.0	1.8955745733616076	0.0
2	6.311218822579016E-4	0.0	0.0	2.4986115318590327	0.0
3	6.311218822579016E-4	0.0	0.0	3.1922144804604664	0.0
4	6.311218822579016E-4	0.0	0.0	6.360446329395133	0.0
5	6.311218822579016E-4	0.0	0.0	6.8792285166111276	0.0
6	6.311218822579016E-4	0.0	0.0	9.096990810865394	0.0
7	6.311218822579016E-4	0.0	0.0	10.832260426133494	0.0
8	6.311218822579016E-4	0.0	0.0	11.789672321518731	0.0
9	6.311218822579016E-4	0.0	0.0	13.367792588104615	0.0
10	6.311218822579016E-4	0.0	0.0	15.419885388266183	0.0
11	6.311218822579016E-4	0.0	0.0	17.82666868625669	0.0
12	6.311218822579016E-4	0.0	0.0	19.00560436231445	0.0
13	6.311218822579016E-4	0.0	0.0	19.57740078764011	0.0
14	9.466828233868524E-4	0.0	0.0	20.108174290619004	0.0
15	9.466828233868524E-4	0.0	0.0	20.513038978087447	0.0
16	9.466828233868524E-4	0.0	0.0	21.317403817025145	0.0
17	9.466828233868524E-4	0.0	0.0	22.30510956275876	0.0
18	9.466828233868524E-4	0.0	0.0	23.63488336867616	0.0
19	9.466828233868524E-4	0.0	0.0	24.17323033424215	0.0
20	9.466828233868524E-4	0.0	0.0	24.69895486216298	0.0
21	9.466828233868524E-4	0.0	0.0	25.25875997172574	0.0
22	9.466828233868524E-4	0.0	0.0	25.81193830152479	0.0
23	9.466828233868524E-4	0.0	0.0	26.357543168736747	0.0
24	9.466828233868524E-4	0.0	0.0	26.760514490558418	0.0
25	9.466828233868524E-4	0.0	0.0	27.108578208623648	0.0
26	9.466828233868524E-4	0.0	0.0	27.414356760577604	0.0
27	9.466828233868524E-4	0.0	0.0	27.734966676764618	0.0
28	9.466828233868524E-4	0.0	0.0	28.062834494597595	0.0
29	0.0012622437645158032	0.0	0.0	28.408689286074928	0.0
30	0.0012622437645158032	0.0	0.0	28.864043724124002	0.0
31	0.0015778047056447541	0.0	0.0	29.197276077956175	0.0
32	0.0015778047056447541	0.0	0.0	29.546602039785924	0.0
33	0.0015778047056447541	0.0	0.0	29.88709229526406	0.0
34	0.0015778047056447541	0.0	0.0	30.217169039684943	0.0
35	0.0015778047056447541	0.0	0.0	30.56681056245582	0.0
36	0.0015778047056447541	0.0	0.0	30.902567403817024	0.0
37	0.0015778047056447541	0.0	0.0	31.234221952943553	0.0
38	0.0015778047056447541	0.0	0.0	31.581654549126526	0.0
39	0.0015778047056447541	0.0	0.0	31.940762900131272	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGCC	45	3.8380676E-10	45.000004	5
GGTACCT	90	0.0	45.000004	8
CGGGTAC	40	6.7884685E-9	45.0	6
AGTCCTC	20	7.025544E-4	45.0	21
CGAACAG	20	7.025544E-4	45.0	1
AATCACG	20	7.025544E-4	45.0	1
GTCGAGA	25	3.8843886E-5	45.0	40
GGTCGAG	25	3.8843886E-5	45.0	39
CGAAACG	35	1.2082091E-7	45.0	1
AGCCCGA	20	7.025544E-4	45.0	15
CAGTTAC	20	7.025544E-4	45.0	16
TATGGAT	20	7.025544E-4	45.0	37
TAGAACG	20	7.025544E-4	45.0	1
ATTACGG	35	1.2082091E-7	45.0	2
ATTACCG	20	7.025544E-4	45.0	1
GATCCGT	20	7.025544E-4	45.0	42
GATCCAG	20	7.025544E-4	45.0	1
CCCAGCG	20	7.025544E-4	45.0	1
TTGTAGC	20	7.025544E-4	45.0	11
TAAGTCT	25	3.8843886E-5	45.0	45
>>END_MODULE