##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552844_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	320144
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.125487280723675	31.0	31.0	33.0	28.0	34.0
2	31.401400619721127	31.0	31.0	34.0	30.0	34.0
3	31.61715665450547	31.0	31.0	34.0	30.0	34.0
4	35.56074453995702	37.0	35.0	37.0	33.0	37.0
5	34.87357564096157	35.0	35.0	37.0	32.0	37.0
6	34.95631340896597	35.0	35.0	37.0	32.0	37.0
7	34.919726747963416	35.0	35.0	37.0	32.0	37.0
8	35.2700597231246	37.0	35.0	37.0	33.0	37.0
9	36.85752036583538	39.0	37.0	39.0	33.0	39.0
10	36.26484019691139	38.0	35.0	39.0	32.0	39.0
11	36.39864873307012	38.0	35.0	39.0	32.0	39.0
12	36.0505584986756	37.0	35.0	39.0	32.0	39.0
13	35.70330226398121	37.0	35.0	39.0	30.0	39.0
14	36.697792243490426	38.0	35.0	40.0	31.0	41.0
15	36.691313908741066	38.0	35.0	40.0	31.0	41.0
16	36.620764406017294	38.0	35.0	40.0	31.0	41.0
17	36.54839072417412	38.0	35.0	40.0	31.0	41.0
18	36.38271527812484	38.0	35.0	40.0	31.0	41.0
19	36.500443550402316	38.0	35.0	40.0	31.0	41.0
20	36.32715278124844	38.0	35.0	40.0	30.0	41.0
21	36.4043367984407	38.0	35.0	40.0	31.0	41.0
22	36.40066969863562	38.0	35.0	40.0	31.0	41.0
23	36.29189677145285	38.0	35.0	40.0	31.0	41.0
24	35.86385501524314	38.0	34.0	40.0	29.0	41.0
25	36.05504398020891	38.0	34.0	40.0	30.0	41.0
26	35.85862299465241	38.0	34.0	40.0	30.0	41.0
27	35.76091383877255	38.0	34.0	40.0	29.0	41.0
28	35.743602878704586	38.0	34.0	40.0	29.0	41.0
29	35.29687890449298	38.0	34.0	40.0	27.0	41.0
30	35.418330501274426	38.0	34.0	40.0	28.0	41.0
31	35.22517679544205	38.0	34.0	40.0	27.0	41.0
32	35.24132265480534	38.0	34.0	40.0	27.0	41.0
33	35.16572854715378	38.0	34.0	40.0	27.0	41.0
34	35.00108701084512	37.0	34.0	40.0	26.0	41.0
35	34.98683717327203	37.0	34.0	40.0	26.0	41.0
36	34.88000399820081	37.0	33.0	40.0	25.0	41.0
37	34.732804612924184	37.0	33.0	40.0	25.0	41.0
38	34.77124981258434	37.0	33.0	40.0	25.0	41.0
39	34.68943350492278	37.0	33.0	40.0	25.0	41.0
40	34.44072042580839	37.0	33.0	40.0	24.0	41.0
41	34.49594557449148	37.0	33.0	40.0	24.0	41.0
42	34.48745876855415	37.0	33.0	40.0	24.0	41.0
43	34.470425808386224	37.0	33.0	40.0	24.0	41.0
44	34.33503048628118	37.0	33.0	40.0	24.0	41.0
45	34.168720950572244	36.0	33.0	40.0	23.0	41.0
46	34.1077671297916	36.0	33.0	40.0	23.0	41.0
47	33.941791818681594	36.0	33.0	39.0	23.0	41.0
48	33.9710567744515	36.0	33.0	39.0	23.0	41.0
49	33.82929556699485	36.0	33.0	39.0	23.0	41.0
50	33.76219451246939	36.0	33.0	39.0	23.0	40.0
51	32.44050177420161	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	7.0
10	4.0
11	13.0
12	2.0
13	7.0
14	41.0
15	54.0
16	77.0
17	168.0
18	349.0
19	683.0
20	1124.0
21	1698.0
22	2130.0
23	2426.0
24	2904.0
25	3365.0
26	3803.0
27	4224.0
28	5382.0
29	6485.0
30	8342.0
31	10889.0
32	14617.0
33	20485.0
34	32131.0
35	34272.0
36	35180.0
37	47849.0
38	53346.0
39	28086.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.806711979609176	35.41781198460693	19.378467189764606	25.397008846019293
2	22.038832525363585	34.923971712729276	19.63460192913189	23.40259383277525
3	20.661952121545305	34.69313808786046	19.438440201909142	25.206469588685092
4	19.08609875556	34.243965215652956	19.42719526213204	27.242740766655004
5	16.494764855814882	36.56354640411815	19.29475486031286	27.64693387975411
6	19.822329951521816	37.967914438502675	20.776275675945826	21.433479934029688
7	73.24204108151332	10.765155679944025	8.953783297516118	7.039019941026538
8	75.54319306312159	9.198360737668049	7.4607051826678	7.797741016542556
9	69.96101754210605	11.97742265980309	12.520615722924683	5.540944075166175
10	36.496389124893795	31.3452696286671	13.796604028187314	18.361737218251786
11	29.33086361137488	27.983969713628866	24.97313708831026	17.71202958668599
12	29.160940076965364	25.13337748013394	28.63555150182418	17.070130941076517
13	24.320930581238443	33.083862261982105	22.661364885801387	19.93384227097806
14	15.929081913139086	34.8205807386676	29.483607376680492	19.76672997151282
15	14.597805987305712	32.79617921935129	31.511444849817583	21.094569943525414
16	16.929256834424507	29.36085011744715	30.558436203708332	23.15145684442001
17	16.054025688440202	29.505784896796445	27.727210255385078	26.712979159378282
18	19.285071717727025	28.013331500824627	26.37125793392973	26.330338847518615
19	21.47221250437303	33.547716027787494	25.40669198860513	19.573379479234347
20	23.794292568344243	30.127380178919488	25.034984257084314	21.043342995651955
21	20.109388275276125	31.76914138637613	25.112761757209256	23.00870858113849
22	18.725635963816284	30.480658703583387	25.202096556549552	25.591608776050776
23	21.074578939477234	30.70836873406967	21.887650557249238	26.32940176920386
24	17.580838622619822	31.10662701784197	26.564920785646457	24.74761357389175
25	20.68694087660553	29.071917637063322	25.02561347393673	25.215528012394422
26	20.21402868709081	33.178819531210955	24.386213703833278	22.22093807786496
27	17.09980508771053	30.460355340096957	25.082150532260485	27.357689039932033
28	21.416612524364037	28.053001149482732	29.23996701484332	21.29041931130991
29	21.26886651007047	29.25308611124994	28.03832025588485	21.439727122794743
30	22.9484232095557	27.531048528162327	24.781036033784797	24.739492228497177
31	24.347168774051678	29.572629816582534	25.11869658653606	20.961504822829728
32	26.337835474036687	26.95162177020341	24.99875056224699	21.71179219351292
33	22.25873356989355	26.841046529061924	28.3125718426708	22.587648058373734
34	21.010857614073668	29.52796241691239	26.048902993652856	23.412276975361088
35	22.828477185266628	24.69513718826528	30.942325953321003	21.534059673147084
36	22.993715328102354	26.5864735868859	30.991678744564943	19.428132340446798
37	20.224024189114896	29.338984956769455	27.400169923534413	23.03682093058124
38	22.258421210455296	26.796691488830028	30.429431755709928	20.515455545004748
39	23.342933180068968	25.702496376630517	29.37396921385377	21.58060122944675
40	23.89674646409116	25.92989404767854	26.29910290369334	23.87425658453696
41	19.323491928632116	24.4449372782248	31.346831425858362	24.884739367284723
42	25.685316607526616	24.497413663851265	26.95661952121545	22.860650207406668
43	20.923396971362887	23.798353241041532	29.994939777100306	25.283310010495274
44	22.35306612024589	23.17176020790644	26.35782647808486	28.11734719376281
45	23.836773451946623	25.99923784297066	27.91025288620121	22.253735818881502
46	21.095819381278424	24.453058623619373	31.7547728522165	22.6963491428857
47	20.81188465190664	25.868359238342748	27.387675546004296	25.93208056374631
48	20.680068968963965	23.822092558348743	33.13477685041731	22.363061622269978
49	21.519691138987458	21.656504572942175	31.657004348043383	25.16679994002699
50	19.924471487830473	23.374481483332502	27.495127192763256	29.205919836073768
51	19.69832325453546	23.45288370233395	32.79617921935129	24.052613823779296
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5515.0
1	4266.5
2	3018.0
3	1994.0
4	970.0
5	991.5
6	1013.0
7	939.0
8	865.0
9	855.0
10	845.0
11	892.0
12	939.0
13	922.0
14	905.0
15	949.5
16	994.0
17	972.5
18	951.0
19	1018.0
20	1085.0
21	1099.5
22	1114.0
23	1082.5
24	1051.0
25	1249.0
26	1855.5
27	2264.0
28	2748.0
29	3232.0
30	3426.0
31	3620.0
32	4188.0
33	4756.0
34	5068.5
35	5381.0
36	5994.0
37	6607.0
38	7373.0
39	8139.0
40	11423.5
41	14708.0
42	19426.0
43	24144.0
44	27883.5
45	31623.0
46	35087.0
47	38551.0
48	35210.0
49	31869.0
50	29702.5
51	27536.0
52	23497.0
53	19458.0
54	17515.5
55	15573.0
56	14298.0
57	13023.0
58	12337.5
59	11652.0
60	10783.5
61	9915.0
62	9061.0
63	8207.0
64	7335.5
65	6464.0
66	5153.5
67	3843.0
68	3435.0
69	3027.0
70	2458.0
71	1889.0
72	1721.0
73	1553.0
74	1246.5
75	755.5
76	571.0
77	461.5
78	352.0
79	323.5
80	295.0
81	207.5
82	120.0
83	91.5
84	63.0
85	40.5
86	18.0
87	14.0
88	10.0
89	10.0
90	10.0
91	10.0
92	10.0
93	7.0
94	4.0
95	3.5
96	3.0
97	2.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	320144.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.85514978033521
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.5826050228862	25.16132958595177
2	10.609251473274412	6.971370924233455
3	3.493945306355608	3.443822890938381
4	1.69931267856168	2.2332469031106643
5	0.9564278020576312	1.5711789345340135
6	0.648203710478334	1.277809799756082
7	0.5095812946566391	1.1719658832840705
8	0.36298868123738837	0.9540837992496597
9	0.33008026161633414	0.9760352791443199
>10	4.086612787163851	32.09088479032067
>50	0.6322382882508183	13.106524655279294
>100	0.07778325943779785	4.6056150058560865
>500	0.00498610637421781	1.3996718577944942
>1k	0.00498610637421781	3.3763151906770275
>5k	9.972212748435621E-4	1.6601444998700143
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCT	5067	1.5827252736268678	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4935	1.5414938277775	No Hit
AAACTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCT	1913	0.5975436053775801	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTT	1232	0.38482682792743267	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1164	0.3635863861262432	No Hit
AATCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCT	1061	0.3314133639862062	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCC	949	0.29642910690189417	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGT	904	0.2823729321805188	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	865	0.27019091408866014	No Hit
CCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC	856	0.267379679144385	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCT	698	0.21802688790044483	No Hit
ACCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG	422	0.1318156829426758	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	398	0.12431905642460893	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCT	391	0.12213254035683943	No Hit
GCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC	382	0.11932130541256435	No Hit
AACTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG	346	0.10807636563546404	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	335	0.1046404118146834	No Hit
GAACTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCT	329	0.10276625518516667	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	321	0.1002673796791444	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.1235943825278623E-4	0.0	0.0	1.885089209855565	0.0
2	3.1235943825278623E-4	0.0	0.0	2.5435429056924383	0.0
3	3.1235943825278623E-4	0.0	0.0	3.243852766255185	0.0
4	3.1235943825278623E-4	0.0	0.0	6.429294317557099	0.0
5	3.1235943825278623E-4	0.0	0.0	6.942500874606427	0.0
6	3.1235943825278623E-4	0.0	0.0	9.21866410115448	0.0
7	3.1235943825278623E-4	0.0	0.0	11.01441851166975	0.0
8	3.1235943825278623E-4	0.0	0.0	11.93837773002149	0.0
9	3.1235943825278623E-4	0.0	0.0	13.582950172422409	0.0
10	3.1235943825278623E-4	0.0	0.0	15.642648308261283	0.0
11	3.1235943825278623E-4	0.0	0.0	18.140899095407068	0.0
12	3.1235943825278623E-4	0.0	0.0	19.31505822379929	0.0
13	3.1235943825278623E-4	0.0	0.0	19.89011194962267	0.0
14	6.247188765055725E-4	0.0	0.0	20.409878054875307	0.0
15	6.247188765055725E-4	0.0	0.0	20.810322854715377	0.0
16	6.247188765055725E-4	0.0	0.0	21.596843920235894	0.0
17	6.247188765055725E-4	0.0	0.0	22.570155929831575	0.0
18	6.247188765055725E-4	0.0	0.0	23.904867809485733	0.0
19	6.247188765055725E-4	0.0	0.0	24.461179969013944	0.0
20	6.247188765055725E-4	0.0	0.0	24.990316857414165	0.0
21	6.247188765055725E-4	0.0	0.0	25.57317956919386	0.0
22	6.247188765055725E-4	0.0	0.0	26.134177120295867	0.0
23	6.247188765055725E-4	0.0	0.0	26.692675795891848	0.0
24	6.247188765055725E-4	0.0	0.0	27.064383527412666	0.0
25	6.247188765055725E-4	0.0	0.0	27.387050827127794	0.0
26	6.247188765055725E-4	0.0	0.0	27.710030486281173	0.0
27	6.247188765055725E-4	0.0	0.0	28.02238992453396	0.0
28	9.370783147583587E-4	0.0	0.0	28.343807786496075	0.0
29	9.370783147583587E-4	0.0	0.0	28.70395821880154	0.0
30	9.370783147583587E-4	0.0	0.0	29.134701884152133	0.0
31	9.370783147583587E-4	0.0	0.0	29.49672647308711	0.0
32	9.370783147583587E-4	0.0	0.0	29.82501624269079	0.0
33	9.370783147583587E-4	0.0	0.0	30.167674546454094	0.0
34	9.370783147583587E-4	0.0	0.0	30.492528362236992	0.0
35	9.370783147583587E-4	0.0	0.0	30.82644060172922	0.0
36	9.370783147583587E-4	0.0	0.0	31.182218001899145	0.0
37	9.370783147583587E-4	0.0	0.0	31.498325753410963	0.0
38	9.370783147583587E-4	0.0	0.0	31.84785596481583	0.0
39	9.370783147583587E-4	0.0	0.0	32.236743465440554	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAT	65	0.0	45.000004	10
ATTACGG	30	2.1605774E-6	45.000004	2
CCATAAT	30	2.1605774E-6	45.000004	11
GAACGTA	65	0.0	45.000004	9
GTCCATA	30	2.1605774E-6	45.000004	9
CCACGAT	60	0.0	45.000004	35
ACAACCG	20	7.0256524E-4	45.0	1
CCGGGTG	25	3.8844788E-5	45.0	5
GTACCGG	25	3.8844788E-5	45.0	2
CGAACAG	25	3.8844788E-5	45.0	1
TCCGCAA	20	7.0256524E-4	45.0	33
CTGTACG	20	7.0256524E-4	45.0	23
GTCGAGA	20	7.0256524E-4	45.0	40
CTCCGAG	20	7.0256524E-4	45.0	16
CAACGGT	20	7.0256524E-4	45.0	32
CGAAACG	20	7.0256524E-4	45.0	1
GGATGCA	20	7.0256524E-4	45.0	8
GGGTCGA	20	7.0256524E-4	45.0	7
CTATATA	25	3.8844788E-5	45.0	39
ACTCTTA	25	3.8844788E-5	45.0	39
>>END_MODULE