SRR3552843_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3552843_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	316594
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGCT	3771	1.1911154349103268	Illumina Single End Adapter 2 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3286	1.0379223864002478	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCC	2289	0.7230080165764354	No Hit
AATCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCT	1934	0.6108770223061712	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGT	1813	0.5726577256675742	No Hit
AAACTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCT	948	0.29943713399495886	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCG	708	0.22363026462914648	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	684	0.21604957769256525	No Hit
AAAAACTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTT	603	0.19046475928160356	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	567	0.17909372887673172	No Hit
CCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC	545	0.17214476585153224	Illumina Single End Adapter 2 (95% over 21bp)
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	540	0.17056545607307783	No Hit
AATGACTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTT	467	0.14750753330764324	No Hit
AATGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTC	452	0.14276960397227995	No Hit
GAACTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCT	447	0.14119029419382553	No Hit
AACTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTG	392	0.12381788663082685	No Hit
TTCCTAGGGGGAGAAAGAAGATTAGAAAATGGAAGTGAAAGACGAAGAAGA	354	0.11181513231457324	No Hit
CAAGGTGGGGTGAGGCCCGGAGTTGGGGAAGCTAGGAGGCTTAAAGCCTAC	345	0.10897237471335529	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	342	0.10802478884628262	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	338	0.10676134102351909	No Hit
GCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC	333	0.10518203124506464	Illumina Single End Adapter 2 (95% over 21bp)
AATGATCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCT	324	0.10233927364384669	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCGAC	60	0.0	45.000004	9
TAGAAGG	30	2.160501E-6	45.000004	2
GAGTACG	30	2.160501E-6	45.000004	1
TCTACGG	30	2.160501E-6	45.000004	2
GTCAATT	30	2.160501E-6	45.000004	15
AAGGGCG	30	2.160501E-6	45.000004	5
TGATCCA	40	6.7884685E-9	45.0	31
TCACGCC	20	7.025535E-4	45.0	43
CGGGTAT	20	7.025535E-4	45.0	6
CTACTCG	20	7.025535E-4	45.0	23
CTATCCC	20	7.025535E-4	45.0	14
TTACTAG	40	6.7884685E-9	45.0	41
ATAGACG	20	7.025535E-4	45.0	1
CGATTAC	40	6.7884685E-9	45.0	38
GGATAAT	20	7.025535E-4	45.0	8
ATCACGC	20	7.025535E-4	45.0	42
CCGTAAC	20	7.025535E-4	45.0	17
TCGTGAC	20	7.025535E-4	45.0	15
CGGATCC	20	7.025535E-4	45.0	6
GTATGGA	20	7.025535E-4	45.0	12

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content