##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552843_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	316594
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.074679242183997	31.0	31.0	33.0	28.0	34.0
2	31.34319033209726	31.0	31.0	34.0	30.0	34.0
3	31.55260365010076	31.0	31.0	34.0	30.0	34.0
4	35.48436799181286	37.0	35.0	37.0	33.0	37.0
5	34.84859473015913	35.0	35.0	37.0	32.0	37.0
6	34.94314484797564	35.0	35.0	37.0	32.0	37.0
7	34.937869953315605	35.0	35.0	37.0	32.0	37.0
8	35.34948230225462	37.0	35.0	37.0	33.0	37.0
9	36.83394189403463	39.0	37.0	39.0	33.0	39.0
10	36.18732825006159	37.0	35.0	39.0	32.0	39.0
11	36.41363386545544	38.0	35.0	39.0	32.0	39.0
12	36.09091770532606	37.0	35.0	39.0	32.0	39.0
13	35.69144077272469	37.0	35.0	39.0	30.0	39.0
14	36.71515568835796	38.0	35.0	40.0	31.0	41.0
15	36.78550130450988	38.0	35.0	40.0	32.0	41.0
16	36.714773495391576	38.0	35.0	40.0	32.0	41.0
17	36.63474355167818	38.0	35.0	40.0	31.0	41.0
18	36.55434404947662	38.0	35.0	40.0	31.0	41.0
19	36.60478404518089	38.0	35.0	40.0	31.0	41.0
20	36.49505044315433	38.0	35.0	40.0	31.0	41.0
21	36.60136010158121	38.0	35.0	40.0	31.0	41.0
22	36.60242771499144	38.0	35.0	40.0	31.0	41.0
23	36.548399527470515	38.0	35.0	40.0	31.0	41.0
24	36.055057897496475	38.0	34.0	40.0	30.0	41.0
25	36.23903485220819	38.0	35.0	40.0	30.0	41.0
26	36.092244325539966	38.0	35.0	40.0	30.0	41.0
27	35.990975823925915	38.0	34.0	40.0	30.0	41.0
28	35.90135315261818	38.0	34.0	40.0	30.0	41.0
29	35.390620163363806	38.0	34.0	40.0	27.0	41.0
30	35.576508082907445	38.0	34.0	40.0	29.0	41.0
31	35.34271022192461	38.0	34.0	40.0	27.0	41.0
32	35.40841266732787	38.0	34.0	40.0	28.0	41.0
33	35.347403930586175	38.0	34.0	40.0	27.0	41.0
34	35.2997056166573	38.0	34.0	40.0	27.0	41.0
35	35.22187407215551	38.0	34.0	40.0	27.0	41.0
36	35.22430305059477	38.0	34.0	40.0	27.0	41.0
37	35.12646165119996	37.0	34.0	40.0	27.0	41.0
38	35.00304806787242	37.0	34.0	40.0	26.0	41.0
39	34.94380815808259	37.0	34.0	40.0	26.0	41.0
40	34.79314516383759	37.0	33.0	40.0	26.0	41.0
41	34.88503572398719	37.0	34.0	40.0	26.0	41.0
42	34.798909644528955	37.0	33.0	40.0	26.0	41.0
43	34.74052572063905	37.0	33.0	40.0	26.0	41.0
44	34.68149112112042	37.0	33.0	40.0	26.0	41.0
45	34.40762932967776	37.0	33.0	40.0	24.0	41.0
46	34.28344504317833	36.0	33.0	40.0	24.0	41.0
47	34.24505518108366	36.0	33.0	40.0	24.0	41.0
48	34.30322116022413	36.0	33.0	40.0	25.0	41.0
49	34.16139914211893	36.0	33.0	39.0	24.0	41.0
50	34.01181323714284	36.0	33.0	39.0	24.0	41.0
51	32.628849567584986	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	7.0
11	4.0
12	6.0
13	12.0
14	23.0
15	39.0
16	82.0
17	134.0
18	281.0
19	489.0
20	846.0
21	1289.0
22	1645.0
23	2072.0
24	2384.0
25	2834.0
26	3211.0
27	3927.0
28	4996.0
29	6331.0
30	8005.0
31	10841.0
32	14531.0
33	20249.0
34	32520.0
35	34703.0
36	36627.0
37	46847.0
38	52404.0
39	29251.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.350354081252327	34.10298363203346	20.417000953903106	25.12966133281111
2	21.862701125100287	35.308312854949875	20.256227218456445	22.572758801493396
3	20.574931931748548	32.74477722256265	21.76825840034871	24.91203244534009
4	21.63464879309147	31.339823243649594	19.80359703595141	27.22193092730753
5	17.616568854747722	36.14724220926486	19.032262140154266	27.20392679583315
6	19.541431612727976	37.2202252727468	22.19625135030986	21.042091764215368
7	75.42183364182517	9.909221273934438	8.586391403501013	6.08255368073937
8	77.30784537925544	7.826111676153054	6.535499725200099	8.330543219391396
9	72.95968969721473	9.615469655141917	11.025793287301717	6.399047360341637
10	37.88479882752042	32.57768624800217	13.31484488019356	16.222670044283845
11	29.81673689330815	26.70012697650619	25.7051618160799	17.777974314105762
12	29.8704334257756	22.658989115397006	30.07858645457589	17.3919910042515
13	24.10247825290435	31.898583043266772	24.581956701643115	19.416982002185765
14	17.286177249095054	34.07234502233144	29.84895481278862	18.792522915784886
15	15.755194349861338	30.758321383222675	32.37395528658155	21.112528980334435
16	16.108959740235125	28.37166844602235	31.603252114695795	23.916119699046728
17	16.733418826636008	27.05420822883567	27.546952879713448	28.665420064814874
18	20.3686740746824	27.122750273220593	26.734555929676496	25.774019722420515
19	22.02094796490142	31.946594060531787	25.39119503212316	20.641262942443632
20	23.831468694921572	29.746299677189082	24.714302861077595	21.70792876681175
21	19.57649228980966	31.65600106129617	26.346677448088084	22.420829200806082
22	19.12891589859568	29.001497185669972	25.749066627920936	26.120520287813413
23	20.673796723879796	30.007517514545444	22.5449629493926	26.773722812182164
24	17.550553706008326	31.31265911546018	27.383652248621264	23.75313492991023
25	19.21261931685376	29.87074928773129	25.127450299121275	25.789181096293678
26	21.754992198209695	33.48673695648054	21.32415649064733	23.434114354662437
27	17.843673600889467	30.453514595980973	26.114203048699597	25.588608754429963
28	20.028490748403318	31.628836933106758	28.330290529826847	20.012381788663085
29	18.20501967819984	32.485770418896124	27.9332520515234	21.375957851380633
30	25.00205310271199	30.675881412787355	23.935071416388183	20.38699406811247
31	25.249057152062264	34.980448144942734	19.09038074000139	20.680113962993612
32	24.496989835562268	29.84895481278862	23.42116401447911	22.232891337170003
33	25.932898286133028	29.579208702628602	22.995382098207802	21.492510913030568
34	21.722774278729222	29.551096988572112	24.168493401643744	24.557635331054918
35	22.352603018376847	29.05013992684637	24.789478006532025	23.807779048244754
36	27.684352830438986	27.686879726084513	26.21843749407759	18.410329949398914
37	22.439780918147534	32.004080936467524	22.812813887818468	22.74332425756647
38	23.851052136174406	29.425383930207143	24.442661579183433	22.280902354435018
39	24.724094581704012	25.720323189953064	27.439559814778548	22.116022413564377
40	26.648009753817192	26.479023607522567	22.38861128132561	24.484355357334632
41	22.745535291256306	22.72058219675673	27.868500350606773	26.66538216138019
42	24.93066830072585	25.980909303398043	23.364624724410444	25.723797671465665
43	21.27109168209126	26.520401523718075	25.842245904849744	26.36626088934092
44	21.972936947636406	24.976152422345336	25.426887433116228	27.624023196902026
45	24.07405067689217	28.916214457633437	25.213680613024884	21.79605425244951
46	21.85669974794216	27.130962684068553	29.62595627207085	21.386381295918433
47	22.595816724258828	26.183376816995903	25.430993638540212	25.78981282020506
48	22.418618167116243	22.32417544236467	32.227395339140976	23.029811051378104
49	23.3384081820881	21.462188165284243	29.637327302475725	25.56207635015193
50	21.163698617156356	23.725654939765125	27.38839017795663	27.722256265121892
51	21.397752326323307	24.751258709893428	30.886245475277484	22.964743488505786
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3695.0
1	2817.0
2	1939.0
3	1287.0
4	635.0
5	721.0
6	807.0
7	774.0
8	741.0
9	677.5
10	614.0
11	644.5
12	675.0
13	681.0
14	687.0
15	683.0
16	679.0
17	751.5
18	824.0
19	819.5
20	815.0
21	827.0
22	839.0
23	895.0
24	951.0
25	1191.5
26	1559.5
27	1687.0
28	1954.5
29	2222.0
30	2770.0
31	3318.0
32	4078.5
33	4839.0
34	5716.0
35	6593.0
36	7155.5
37	7718.0
38	8541.0
39	9364.0
40	10102.0
41	10840.0
42	14747.0
43	18654.0
44	24674.0
45	30694.0
46	33938.0
47	37182.0
48	37135.5
49	37089.0
50	34454.0
51	31819.0
52	27568.5
53	23318.0
54	19902.0
55	16486.0
56	15335.5
57	14185.0
58	12730.5
59	11276.0
60	10761.5
61	10247.0
62	8941.0
63	7635.0
64	6477.5
65	5320.0
66	4407.0
67	3494.0
68	3034.5
69	2575.0
70	2203.0
71	1831.0
72	1352.5
73	874.0
74	708.5
75	554.0
76	565.0
77	504.0
78	443.0
79	335.0
80	227.0
81	160.0
82	93.0
83	58.0
84	23.0
85	28.0
86	33.0
87	46.0
88	59.0
89	33.0
90	7.0
91	4.5
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	2.0
98	3.0
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	316594.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.116183820222176
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.4369318314428	25.644156690040738
2	10.005544641781944	6.626909111573798
3	3.22190290617794	3.200913866756901
4	1.6609977823601505	2.2002363154248052
5	0.9578876353202697	1.5860791505191996
6	0.6136110034359693	1.2192272870337926
7	0.5128347260560971	1.1888190340226896
8	0.4110654505787727	1.0890335218807272
9	0.337233244306976	1.0051090297883738
>10	4.071742091155875	31.474251457525188
>50	0.692425633476625	14.560533635044584
>100	0.06585027638250325	4.360428482837509
>500	0.006984120222386709	1.5182366354958214
>1k	0.004988657301704792	4.326065782055885
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGCT	3771	1.1911154349103268	Illumina Single End Adapter 2 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3286	1.0379223864002478	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCC	2289	0.7230080165764354	No Hit
AATCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCT	1934	0.6108770223061712	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGT	1813	0.5726577256675742	No Hit
AAACTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCT	948	0.29943713399495886	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCG	708	0.22363026462914648	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	684	0.21604957769256525	No Hit
AAAAACTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTT	603	0.19046475928160356	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	567	0.17909372887673172	No Hit
CCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC	545	0.17214476585153224	Illumina Single End Adapter 2 (95% over 21bp)
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	540	0.17056545607307783	No Hit
AATGACTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTT	467	0.14750753330764324	No Hit
AATGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTC	452	0.14276960397227995	No Hit
GAACTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCT	447	0.14119029419382553	No Hit
AACTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTG	392	0.12381788663082685	No Hit
TTCCTAGGGGGAGAAAGAAGATTAGAAAATGGAAGTGAAAGACGAAGAAGA	354	0.11181513231457324	No Hit
CAAGGTGGGGTGAGGCCCGGAGTTGGGGAAGCTAGGAGGCTTAAAGCCTAC	345	0.10897237471335529	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	342	0.10802478884628262	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	338	0.10676134102351909	No Hit
GCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC	333	0.10518203124506464	Illumina Single End Adapter 2 (95% over 21bp)
AATGATCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCT	324	0.10233927364384669	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.5445649633284269	0.0
2	0.0	0.0	0.0	2.055313745680588	0.0
3	0.0	0.0	0.0	2.6428169832656336	0.0
4	0.0	0.0	0.0	5.591704201595735	0.0
5	0.0	0.0	0.0	6.203212947813288	0.0
6	0.0	0.0	0.0	8.04247711580131	0.0
7	0.0	0.0	0.0	9.380784222063589	0.0
8	0.0	0.0	0.0	10.064625356134355	0.0
9	0.0	0.0	0.0	11.91905089799554	0.0
10	0.0	0.0	0.0	13.893819844974953	0.0
11	0.0	0.0	0.0	16.451038238248355	0.0
12	0.0	0.0	0.0	17.584982659178632	0.0
13	0.0	0.0	0.0	18.146585216397025	0.0
14	0.0	0.0	0.0	18.73345673007069	0.0
15	0.0	0.0	0.0	19.074271780261153	0.0
16	0.0	0.0	0.0	19.730632924186814	0.0
17	0.0	0.0	0.0	20.570194002413185	0.0
18	0.0	0.0	0.0	21.801739767651945	0.0
19	0.0	0.0	0.0	22.338073368415067	0.0
20	0.0	0.0	0.0	22.807760096527414	0.0
21	0.0	0.0	0.0	23.33872404404379	0.0
22	0.0	0.0	0.0	23.859896270933753	0.0
23	0.0	0.0	0.0	24.431290548778563	0.0
24	0.0	0.0	0.0	24.844122124866548	0.0
25	0.0	0.0	0.0	25.173566144652142	0.0
26	0.0	0.0	0.0	25.50174671661497	0.0
27	0.0	0.0	0.0	25.836876251602998	0.0
28	0.0	0.0	0.0	26.167583719211358	0.0
29	0.0	0.0	0.0	26.53303600194571	0.0
30	0.0	0.0	0.0	26.95439585083735	0.0
31	0.0	0.0	0.0	27.291104695603835	0.0
32	0.0	0.0	0.0	27.624339058857718	0.0
33	0.0	0.0	0.0	27.96894445251647	0.0
34	0.0	0.0	0.0	28.294598128833776	0.0
35	0.0	0.0	0.0	28.700480741896563	0.0
36	0.0	0.0	0.0	29.01413166389761	0.0
37	0.0	0.0	0.0	29.343575683683202	0.0
38	0.0	0.0	0.0	29.668597636089125	0.0
39	0.0	0.0	0.0	30.020467854728768	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCGAC	60	0.0	45.000004	9
TAGAAGG	30	2.160501E-6	45.000004	2
GAGTACG	30	2.160501E-6	45.000004	1
TCTACGG	30	2.160501E-6	45.000004	2
GTCAATT	30	2.160501E-6	45.000004	15
AAGGGCG	30	2.160501E-6	45.000004	5
TGATCCA	40	6.7884685E-9	45.0	31
TCACGCC	20	7.025535E-4	45.0	43
CGGGTAT	20	7.025535E-4	45.0	6
CTACTCG	20	7.025535E-4	45.0	23
CTATCCC	20	7.025535E-4	45.0	14
TTACTAG	40	6.7884685E-9	45.0	41
ATAGACG	20	7.025535E-4	45.0	1
CGATTAC	40	6.7884685E-9	45.0	38
GGATAAT	20	7.025535E-4	45.0	8
ATCACGC	20	7.025535E-4	45.0	42
CCGTAAC	20	7.025535E-4	45.0	17
TCGTGAC	20	7.025535E-4	45.0	15
CGGATCC	20	7.025535E-4	45.0	6
GTATGGA	20	7.025535E-4	45.0	12
>>END_MODULE