FastQCFastQC Report
Sat 18 Jun 2016
SRR3552841_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552841_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences424626
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCT46681.0993203430783796Illumina Single End Adapter 1 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39500.9302303674292199No Hit
AAACTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCT16940.39893930187977183No Hit
AAAAACTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTT11760.27694959799918045No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9290.21878076236499883No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8320.19593713055724332No Hit
CCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC7860.18510406805047266No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCC6650.15660840363048895No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG6570.15472439275974623No Hit
AATCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCT5990.14106531394686148No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCT5970.1405943112291758No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGT5390.12693523241629104No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5270.12410921611017696No Hit
ACCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTG4400.10362059789084982No Hit
GAAAAACTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCT4380.10314959517316415No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4340.10220758973779279No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG207.0282567E-445.01
AATCCGG253.8866365E-545.02
ATCTCGC253.8866365E-545.010
TTAGCAC253.8866365E-545.034
AAGACCG351.2095006E-745.01
ACCGACG207.0282567E-445.01
ATTAACG351.2095006E-745.01
TACGGGT502.1827873E-1145.04
TCTAGTC253.8866365E-545.012
ATGTCGT207.0282567E-445.036
TCGATGG253.8866365E-545.02
ATCACGC253.8866365E-545.042
TCGATAG253.8866365E-545.01
TCTAGCG253.8866365E-545.01
ATCCGAT351.2095006E-745.024
GCCGATG406.7975634E-945.09
CGGATGA253.8866365E-545.026
CTACGCG351.2095006E-745.01
CGCAGAC351.2095006E-745.037
TACTAGA207.0282567E-445.030