##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552833_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	368593
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.15010051737282	31.0	31.0	33.0	28.0	34.0
2	31.42348064124929	31.0	31.0	34.0	30.0	34.0
3	31.59295211791868	31.0	31.0	34.0	30.0	34.0
4	35.52184658959882	37.0	35.0	37.0	33.0	37.0
5	34.90616479423104	35.0	35.0	37.0	32.0	37.0
6	35.01592542451973	35.0	35.0	37.0	32.0	37.0
7	34.97355348582312	36.0	35.0	37.0	32.0	37.0
8	35.41658957169561	37.0	35.0	37.0	33.0	37.0
9	36.83299194504508	39.0	37.0	39.0	33.0	39.0
10	36.27826898503227	38.0	35.0	39.0	32.0	39.0
11	36.4685276171821	38.0	35.0	39.0	32.0	39.0
12	36.090576869338264	37.0	35.0	39.0	32.0	39.0
13	35.76453432376632	37.0	35.0	39.0	30.0	39.0
14	36.738687386901	39.0	35.0	40.0	31.0	41.0
15	36.79622239163522	38.0	35.0	40.0	32.0	41.0
16	36.70173877420353	38.0	35.0	40.0	31.0	41.0
17	36.60707609748422	38.0	35.0	40.0	31.0	41.0
18	36.53605738578866	38.0	35.0	40.0	31.0	41.0
19	36.58547503615098	38.0	35.0	40.0	31.0	41.0
20	36.45203788460443	38.0	35.0	40.0	30.0	41.0
21	36.56881167032472	38.0	35.0	40.0	31.0	41.0
22	36.56731137053607	38.0	35.0	40.0	31.0	41.0
23	36.53806773324507	38.0	35.0	40.0	31.0	41.0
24	36.07595912022203	38.0	34.0	40.0	30.0	41.0
25	36.22751924209087	38.0	35.0	40.0	30.0	41.0
26	36.14522793433407	38.0	35.0	40.0	30.0	41.0
27	36.06024531122403	38.0	34.0	40.0	30.0	41.0
28	35.985832612122316	38.0	34.0	40.0	30.0	41.0
29	35.55325792947777	38.0	34.0	40.0	28.0	41.0
30	35.634002816114254	38.0	34.0	40.0	29.0	41.0
31	35.4730692118407	38.0	34.0	40.0	28.0	41.0
32	35.45355988854916	38.0	34.0	40.0	28.0	41.0
33	35.369627203989225	38.0	34.0	40.0	28.0	41.0
34	35.27635901929771	38.0	34.0	40.0	27.0	41.0
35	35.21573931138139	38.0	34.0	40.0	27.0	41.0
36	35.14578139031398	37.0	34.0	40.0	27.0	41.0
37	35.030931135425796	37.0	34.0	40.0	26.0	41.0
38	34.86154104934169	37.0	33.0	40.0	26.0	41.0
39	34.830512245213555	37.0	33.0	40.0	26.0	41.0
40	34.687229545867666	37.0	33.0	40.0	25.0	41.0
41	34.77728822847965	37.0	33.0	40.0	26.0	41.0
42	34.70834497670873	37.0	33.0	40.0	26.0	41.0
43	34.681895749512336	37.0	33.0	40.0	26.0	41.0
44	34.55980987159278	37.0	33.0	40.0	25.0	41.0
45	34.38476856587076	36.0	33.0	40.0	24.0	41.0
46	34.322244318258896	36.0	33.0	40.0	24.0	41.0
47	34.212383306248356	36.0	33.0	40.0	24.0	41.0
48	34.237207434758666	36.0	33.0	40.0	24.0	41.0
49	34.1731720352801	36.0	33.0	39.0	24.0	41.0
50	34.06289593128464	36.0	33.0	39.0	24.0	41.0
51	32.83957915641371	35.0	31.0	39.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	8.0
10	6.0
11	11.0
12	9.0
13	15.0
14	31.0
15	57.0
16	102.0
17	183.0
18	341.0
19	644.0
20	1066.0
21	1729.0
22	2139.0
23	2535.0
24	2961.0
25	3395.0
26	3867.0
27	4623.0
28	5571.0
29	7155.0
30	9476.0
31	12468.0
32	16496.0
33	23982.0
34	37039.0
35	38190.0
36	40675.0
37	54145.0
38	62205.0
39	37467.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.266798338546852	33.94448619480023	19.80043028489418	25.98828518175874
2	24.542245783289427	33.831624583212374	18.572246353023523	23.053883280474672
3	20.704950989302564	31.556757724644797	21.33084459010345	26.40744669594919
4	20.829749886731435	32.930630804166114	18.797426972297355	27.442192336805093
5	17.110471441400136	35.68488821003112	17.67803512275057	29.52660522581818
6	19.14496477144167	36.96841773989197	21.427156782684424	22.459460705981936
7	77.9152072882556	9.700943859487293	6.84901775128665	5.534831100970447
8	79.94563108903317	7.085321750548708	5.669939472534747	7.299107687883384
9	74.93902488652796	9.807294224252766	9.963021544087923	5.290659345131351
10	38.17272710008058	32.32155792432304	12.93052228338574	16.57519269221065
11	31.30526081613053	27.078376420604837	23.408474930343225	18.207887832921408
12	30.215440879235363	23.66105704666122	28.583559644377406	17.539942429726015
13	26.202071119093418	30.986209721834108	22.320011503202718	20.491707655869753
14	17.847598842083272	32.79226680919062	30.323961659608294	19.03617268911781
15	17.035049499040948	29.162518007666993	31.360877716071656	22.441554777220404
16	19.713071056693966	27.466880814339934	30.278111629900728	22.541936499065365
17	18.5879818661776	27.215112603874736	26.804904054065055	27.392001475882616
18	23.198758522272534	27.32905942326631	24.671656813884148	24.800525240577002
19	22.47031278401923	31.90890765695496	25.241119608891104	20.3796599501347
20	24.667858586571096	30.707040014324743	23.512654879501238	21.112446519602923
21	21.838721842248766	29.976966464365844	25.95193072033381	22.232380973051576
22	20.2630001112338	29.218948813460916	25.509979842265047	25.008071233040237
23	20.57635386456064	29.260186710002635	23.287474260227405	26.875985165209322
24	19.316427604430903	30.513059119408126	27.486414554807066	22.684098721353905
25	20.070104424120913	29.48916555658952	25.010512950598628	25.43021706869094
26	21.857712978814032	31.564354179270904	22.864514518723905	23.713418323191163
27	18.42058856245235	29.20999584908015	25.34394304829446	27.025472540173034
28	19.52587271055066	31.227668458163883	27.109847446912987	22.136611384372465
29	20.9922597553399	30.920554649708485	25.872439248710638	22.214746346240975
30	22.970593581538445	29.470174420024254	24.737583187960706	22.821648810476596
31	25.65376987625918	30.66742992948862	20.906799640796216	22.772000553455978
32	25.14616392606479	29.465833588809335	22.272804963740494	23.115197521385376
33	25.43482920185679	28.69479344425966	22.543835612721892	23.32654174116166
34	19.934182146703815	29.80360451772009	23.90767052005871	26.354542815517384
35	24.224279896796737	28.852148575800406	24.644797920741848	22.278773606661005
36	26.00347809101095	27.919955072396924	24.483644561888042	21.592922274704076
37	21.866123339292933	31.487033123255188	22.381597046064357	24.26524649138752
38	24.012393073118588	29.950378873174476	23.751400596321687	22.285827457385246
39	22.203080362350885	26.858621840349656	24.701771330437637	26.236526466861825
40	26.870016522288815	26.41748486813369	21.94615741481797	24.76634119475953
41	22.587243924871064	22.44101217331854	26.910169211026798	28.0615746907836
42	25.699891207917673	26.262842756102263	23.124150485766144	24.91311555021392
43	23.16104755109294	24.445119684855655	26.832848155011085	25.56098460904032
44	22.964896240568866	24.84990219564669	24.647510940251173	27.537690623533273
45	24.48201675018245	27.483972837248672	24.99911826865947	23.034892143909406
46	21.690862278990647	25.512692861774365	28.27129109885429	24.52515376038069
47	22.36206330559723	27.18635459707591	24.374038573711385	26.077543523615475
48	22.189515264804268	23.805932288459086	30.44848925508623	23.556063191650413
49	23.703651452957597	22.483606579614914	28.185017078457815	25.627724888969677
50	21.182442422943463	24.069095180863446	26.636696844487005	28.111765551706082
51	20.383186875496822	24.196335795850707	29.816355709413905	25.604121619238562
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3448.0
1	2799.0
2	2150.0
3	1502.5
4	855.0
5	1032.5
6	1210.0
7	1086.0
8	962.0
9	919.5
10	877.0
11	856.0
12	835.0
13	931.0
14	1027.0
15	990.0
16	953.0
17	1042.5
18	1132.0
19	1063.0
20	994.0
21	991.0
22	988.0
23	1322.0
24	1656.0
25	1799.0
26	2357.0
27	2772.0
28	3311.0
29	3850.0
30	4344.0
31	4838.0
32	5455.0
33	6072.0
34	6750.0
35	7428.0
36	7791.0
37	8154.0
38	9325.0
39	10496.0
40	11559.5
41	12623.0
42	16101.5
43	19580.0
44	20673.5
45	21767.0
46	27459.0
47	33151.0
48	32559.0
49	31967.0
50	34202.5
51	36438.0
52	33487.5
53	30537.0
54	27504.0
55	24471.0
56	23381.0
57	22291.0
58	20012.0
59	17733.0
60	16699.5
61	15666.0
62	13390.0
63	11114.0
64	9593.5
65	8073.0
66	7202.5
67	6332.0
68	5479.5
69	4627.0
70	3851.5
71	3076.0
72	2678.5
73	2281.0
74	1852.0
75	1295.5
76	1168.0
77	874.5
78	581.0
79	468.0
80	355.0
81	315.0
82	275.0
83	204.5
84	134.0
85	158.0
86	182.0
87	113.0
88	44.0
89	44.5
90	45.0
91	27.0
92	9.0
93	7.0
94	5.0
95	4.0
96	3.0
97	2.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	368593.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.765733255315105
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.10350290403633	21.891730643326078
2	10.01766744021902	5.763310988515913
3	3.278400958639036	2.8291682244053957
4	1.5418880770625742	1.7741416453733103
5	0.96824729195583	1.392617166279131
6	0.6622756327262302	1.1430506515498666
7	0.4859781134306772	0.978566174520769
8	0.42622279746812547	0.9808489039441833
9	0.3575086640635774	0.9255598980225233
>10	5.062878157503061	35.197615219344144
>50	1.009787256705524	18.64697288044736
>100	0.07468840656105946	3.733175877622976
>500	0.004979227104070631	0.9344398183552908
>1k	0.005975072524884757	3.8088019082930553
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGCT	3259	0.8841730580884607	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2964	0.8041389825634235	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCC	2269	0.6155841266654549	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGT	1898	0.5149311028695608	No Hit
AATCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCT	1853	0.5027225150776059	No Hit
AAACTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCT	1053	0.28568095433174256	No Hit
AAAAACTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTT	862	0.23386228170366774	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	716	0.19425219686754766	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	574	0.15572731983515695	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	568	0.15409950812956297	No Hit
AACTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTG	542	0.1470456574053224	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	468	0.12696931303633002	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCT	439	0.1191015564592925	No Hit
AATGACTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTT	418	0.11340421548971359	No Hit
CCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC	418	0.11340421548971359	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	376	0.10200953355055575	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.426039018646583E-4	0.0	0.0	1.1166788300374668	0.0
2	5.426039018646583E-4	0.0	0.0	1.411855352651841	0.0
3	5.426039018646583E-4	0.0	0.0	1.9077953189561385	0.0
4	5.426039018646583E-4	0.0	0.0	4.055692864487389	0.0
5	5.426039018646583E-4	0.0	0.0	4.376914374391267	0.0
6	5.426039018646583E-4	0.0	0.0	5.993874001947948	0.0
7	5.426039018646583E-4	0.0	0.0	7.181633943129685	0.0
8	5.426039018646583E-4	0.0	0.0	7.781753858591997	0.0
9	5.426039018646583E-4	0.0	0.0	9.182757133206545	0.0
10	5.426039018646583E-4	0.0	0.0	10.417452311899575	0.0
11	5.426039018646583E-4	0.0	0.0	12.542289191601578	0.0
12	5.426039018646583E-4	0.0	0.0	13.381697427786204	0.0
13	5.426039018646583E-4	0.0	0.0	13.712414505972712	0.0
14	5.426039018646583E-4	0.0	0.0	14.118824828469341	0.0
15	5.426039018646583E-4	0.0	0.0	14.402064065242692	0.0
16	5.426039018646583E-4	0.0	0.0	14.961759990016088	0.0
17	5.426039018646583E-4	0.0	0.0	15.640014867346911	0.0
18	5.426039018646583E-4	0.0	0.0	16.67665962185934	0.0
19	5.426039018646583E-4	0.0	0.0	17.07248916826961	0.0
20	5.426039018646583E-4	0.0	0.0	17.484325529784886	0.0
21	5.426039018646583E-4	0.0	0.0	17.91135480055237	0.0
22	5.426039018646583E-4	0.0	0.0	18.328617201086292	0.0
23	5.426039018646583E-4	0.0	0.0	18.750491734786063	0.0
24	5.426039018646583E-4	0.0	0.0	19.04919518276256	0.0
25	5.426039018646583E-4	0.0	0.0	19.311815471265053	0.0
26	5.426039018646583E-4	0.0	0.0	19.534554372980494	0.0
27	5.426039018646583E-4	0.0	0.0	19.850078541914794	0.0
28	5.426039018646583E-4	0.0	0.0	20.121109190896192	0.0
29	5.426039018646583E-4	0.0	0.0	20.41791352521616	0.0
30	5.426039018646583E-4	0.0	0.0	20.799635370177946	0.0
31	5.426039018646583E-4	0.0	0.0	21.05981394112205	0.0
32	5.426039018646583E-4	0.0	0.0	21.325418551084802	0.0
33	5.426039018646583E-4	0.0	0.0	21.591023161047552	0.0
34	5.426039018646583E-4	0.0	0.0	21.862053810028947	0.0
35	5.426039018646583E-4	0.0	0.0	22.181918810178164	0.0
36	8.139058527969875E-4	0.0	0.0	22.439655663563876	0.0
37	8.139058527969875E-4	0.0	0.0	22.714213237907394	0.0
38	8.139058527969875E-4	0.0	0.0	22.994468153220488	0.0
39	8.139058527969875E-4	0.0	0.0	23.373205676722023	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATACCG	70	0.0	45.000004	1
ACATCCG	35	1.2089185E-7	45.000004	1
CGGAAAC	20	7.027043E-4	45.0	35
AGTCCGG	20	7.027043E-4	45.0	2
CGAAATC	25	3.885631E-5	45.0	35
CCTAGTA	20	7.027043E-4	45.0	43
TTAGCAG	25	3.885631E-5	45.0	1
TTTCGCG	20	7.027043E-4	45.0	36
TTGTCGT	25	3.885631E-5	45.0	28
ATAGCCG	20	7.027043E-4	45.0	1
TGAACTC	20	7.027043E-4	45.0	9
GGTTTAC	20	7.027043E-4	45.0	17
ATCACGC	40	6.7939254E-9	45.0	42
GACCCGG	20	7.027043E-4	45.0	2
GAAAGTG	25	3.885631E-5	45.0	9
ATCCGCG	20	7.027043E-4	45.0	1
ATATCCG	25	3.885631E-5	45.0	1
GTTCGCT	20	7.027043E-4	45.0	19
CTTGCCG	20	7.027043E-4	45.0	43
ACCAGTC	20	7.027043E-4	45.0	39
>>END_MODULE