SRR3552831_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3552831_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	347770
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCT	5628	1.6183109526411135	Illumina PCR Primer Index 5 (95% over 24bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4022	1.1565114874773557	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCC	1906	0.5480633752192541	No Hit
AAACTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCT	1845	0.5305230468413031	Illumina PCR Primer Index 5 (95% over 21bp)
AATCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCT	1795	0.5161457284987204	Illumina PCR Primer Index 5 (95% over 21bp)
AATGATACCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGT	1687	0.48509072087874167	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTT	1430	0.41119130459786646	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	968	0.27834488311240185	No Hit
CCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC	774	0.22256088794318082	Illumina PCR Primer Index 5 (95% over 23bp)
AAAAAACTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCT	750	0.21565977513874113	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	737	0.2119216723696696	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	520	0.1495241107628605	No Hit
AACTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTG	495	0.14233545159156913	Illumina PCR Primer Index 5 (95% over 22bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	475	0.13658452425453604	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCT	448	0.12882077234954137	No Hit
ACCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTG	434	0.1247951232136182	Illumina PCR Primer Index 5 (95% over 22bp)
AATGATCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCT	397	0.11415590764010695	No Hit
AATGACTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTT	392	0.1127181758058487	No Hit
TACCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCT	386	0.11099289760473877	Illumina PCR Primer Index 5 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC	379	0.10898007303677719	Illumina PCR Primer Index 5 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC	365	0.10495442390085401	Illumina PCR Primer Index 5 (95% over 23bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTGATC	65	0.0	45.000004	16
CGGGTTA	25	3.8851755E-5	45.000004	6
GATCGGG	50	2.1827873E-11	45.000004	3
CGCGGTA	25	3.8851755E-5	45.000004	10
TCGGGCG	25	3.8851755E-5	45.000004	5
TAGCCCG	25	3.8851755E-5	45.000004	1
CGCTATA	25	3.8851755E-5	45.000004	33
TAACACG	25	3.8851755E-5	45.000004	1
TCGCTCG	25	3.8851755E-5	45.000004	21
ACGCTAT	25	3.8851755E-5	45.000004	32
CGTACCA	25	3.8851755E-5	45.000004	45
ATGCGAG	25	3.8851755E-5	45.000004	1
CGGTCCC	25	3.8851755E-5	45.000004	20
ATCGGGC	50	2.1827873E-11	45.000004	4
ATACCCG	25	3.8851755E-5	45.000004	1
ACGACGT	25	3.8851755E-5	45.000004	23
TCGTTTA	20	7.026493E-4	45.0	38
ACCGGTC	20	7.026493E-4	45.0	32
ACCGGGT	45	3.8380676E-10	45.0	4
ATAGGAC	20	7.026493E-4	45.0	16

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content