##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552831_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	347770
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.879526698680163	31.0	30.0	33.0	28.0	34.0
2	31.15245420824108	31.0	31.0	34.0	28.0	34.0
3	31.37074790810018	31.0	31.0	34.0	30.0	34.0
4	35.391928573482474	37.0	35.0	37.0	33.0	37.0
5	34.67670586882135	35.0	35.0	37.0	32.0	37.0
6	34.788512522644275	35.0	35.0	37.0	32.0	37.0
7	34.79032406475544	35.0	35.0	37.0	32.0	37.0
8	35.24068493544584	37.0	35.0	37.0	33.0	37.0
9	36.70303648963395	39.0	37.0	39.0	32.0	39.0
10	35.982022601144436	37.0	35.0	39.0	31.0	39.0
11	36.273108663772035	37.0	35.0	39.0	32.0	39.0
12	36.00324064755442	37.0	35.0	39.0	32.0	39.0
13	35.61383960663657	37.0	35.0	39.0	30.0	39.0
14	36.61110504068781	38.0	35.0	40.0	31.0	41.0
15	36.6696322281968	38.0	35.0	40.0	31.0	41.0
16	36.53297294188688	38.0	35.0	40.0	31.0	41.0
17	36.48751186128763	38.0	35.0	40.0	31.0	41.0
18	36.391390861776465	38.0	35.0	40.0	31.0	41.0
19	36.44173160422118	38.0	35.0	40.0	31.0	41.0
20	36.29029818558242	38.0	34.0	40.0	30.0	41.0
21	36.400109267619406	38.0	35.0	40.0	31.0	41.0
22	36.37918451850361	38.0	35.0	40.0	30.0	41.0
23	36.293052879776866	38.0	35.0	40.0	30.0	41.0
24	35.769499956868046	38.0	34.0	40.0	29.0	41.0
25	36.06646634269776	38.0	34.0	40.0	30.0	41.0
26	35.95197400580844	38.0	34.0	40.0	30.0	41.0
27	35.789173879288036	38.0	34.0	40.0	29.0	41.0
28	35.92147683814015	38.0	34.0	40.0	30.0	41.0
29	35.447890847399144	38.0	34.0	40.0	27.0	41.0
30	35.339215573511225	38.0	34.0	40.0	27.0	41.0
31	35.350792190240675	38.0	34.0	40.0	27.0	41.0
32	35.334189263018665	38.0	34.0	40.0	27.0	41.0
33	35.17607326681427	38.0	34.0	40.0	27.0	41.0
34	35.2636225091296	38.0	34.0	40.0	27.0	41.0
35	35.133502027201885	38.0	34.0	40.0	27.0	41.0
36	35.12087011530609	38.0	34.0	40.0	26.0	41.0
37	34.962676481582655	38.0	34.0	40.0	26.0	41.0
38	34.88957644420163	38.0	34.0	40.0	25.0	41.0
39	34.83394484860684	37.0	34.0	40.0	25.0	41.0
40	34.5865830865227	37.0	33.0	40.0	24.0	41.0
41	34.748857003191766	37.0	33.0	40.0	25.0	41.0
42	34.688883457457514	37.0	33.0	40.0	25.0	41.0
43	34.63285504787647	37.0	33.0	40.0	25.0	41.0
44	34.51140983983667	37.0	33.0	40.0	24.0	41.0
45	34.3324869885269	37.0	33.0	40.0	23.0	41.0
46	34.28172067745924	37.0	33.0	40.0	23.0	41.0
47	34.173088535526354	37.0	33.0	40.0	23.0	41.0
48	34.267708543002556	37.0	33.0	40.0	24.0	41.0
49	34.18090117031371	37.0	33.0	40.0	24.0	41.0
50	34.029973833280614	36.0	33.0	40.0	23.0	41.0
51	32.82483825516864	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	2.0
10	3.0
11	3.0
12	12.0
13	20.0
14	19.0
15	44.0
16	96.0
17	198.0
18	378.0
19	751.0
20	1182.0
21	1732.0
22	2341.0
23	2819.0
24	3133.0
25	3411.0
26	3809.0
27	4485.0
28	5450.0
29	6744.0
30	8821.0
31	11736.0
32	15549.0
33	21766.0
34	33614.0
35	37858.0
36	41773.0
37	54049.0
38	57947.0
39	28021.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.410529947954107	35.67990338442073	19.998562268165742	24.911004399459415
2	20.57221727003479	35.90505218966558	20.252465710095752	23.27026483020387
3	20.06412283980792	34.23210742732265	21.817580584869308	23.886189148000113
4	18.963107801132935	34.6476119274233	20.218247692440407	26.171032579003363
5	15.695143341863876	38.04353451994134	19.422606895361877	26.838715242832905
6	19.93386433562412	37.15070305086695	21.268942116916353	21.646490496592573
7	73.56183684619145	10.645541593581966	8.938953906317394	6.853667653909193
8	75.7515024297668	8.417632343215343	7.3922419990223425	8.438623227995516
9	70.67602150846824	11.28418207435949	12.472036115823675	5.567760301348593
10	33.91120568191621	36.57503522442994	13.164735313569311	16.349023780084536
11	25.127526813698708	26.868044972251777	30.38905023435029	17.615377979699225
12	26.748425683641486	25.853006297265434	30.45116024959025	16.94740776950283
13	22.374557897460964	33.67282974379619	24.56278574920206	19.38982660954079
14	15.307530839347844	35.68622940449148	30.336716795583285	18.669522960577392
15	13.726313368030596	32.35989303275153	32.57411507605602	21.339678523161858
16	15.383443080196683	30.770911809529288	31.778186732610635	22.0674583776634
17	14.538919400753372	30.383586853380105	28.13957500646979	26.93791873939673
18	17.768640193231157	29.471202231359804	26.978462777122814	25.781694798286225
19	19.92552549098542	33.98625528366449	26.619317364925095	19.468901860424996
20	21.31523708197947	31.941513068982374	25.696581073698134	21.046668775340024
21	17.77381602783449	33.22339477240705	26.05141329039308	22.951375909365385
22	17.599275383155533	31.307185783707624	26.38180406590563	24.711734767231217
23	19.166115536130203	31.75662075509676	22.477499496793858	26.59976421197918
24	15.171521407827013	32.991057307990914	28.513960376110646	23.32346090807143
25	19.05023435028898	30.08885182735716	26.890473588866204	23.97044023348765
26	19.621876527590075	34.21427955257785	22.35788020818357	23.805963711648502
27	15.14017885384018	34.287603876125026	25.028898409868596	25.543318860166202
28	21.24018748023119	29.32541622336602	28.77016418897547	20.66423210742732
29	19.672772234522817	29.983897403456304	26.0905195962849	24.252810765735973
30	21.700261667193836	34.48198522011675	22.851884866434713	20.96586824625471
31	23.695833453144317	30.645541593581964	23.715961698823936	21.94266325444978
32	24.817551830232627	29.851338528337696	22.975817350547775	22.355292290881902
33	21.262328550478767	31.755470569629352	25.55769617850878	21.4245047013831
34	20.12709549414843	27.549242315323347	27.96187135175547	24.36179083877275
35	21.013313396785232	29.458837737585185	27.91988958219513	21.607959283434454
36	21.366707881645915	28.051010725479486	30.99404779020617	19.58823360266843
37	18.961670069298677	32.024038876268804	27.651608821922537	21.362682232509993
38	22.054518791155072	29.531299422031804	29.830635189924376	18.58354659688875
39	22.05566897662248	26.999166115536134	29.803318285073466	21.141846622767922
40	23.92500790752509	28.33194352589355	25.95163470109555	21.791413865485808
41	19.729418868792592	23.13770595508526	33.20959254679817	23.923282629323978
42	24.93717111884291	25.033211605371363	27.147540040831586	22.88207723495414
43	19.94795410759985	25.41449808781666	29.33260488253731	25.30494292204618
44	21.13983379819996	25.22586767116197	27.386203525318457	26.24809500531961
45	23.880725767029933	27.63608131811254	27.00089139373724	21.48230152112028
46	19.914311182678208	26.898237340771196	31.363257325243694	21.824194151306898
47	20.27403168760963	26.73146044799724	26.551743968714952	26.44276389567818
48	20.422980705638786	22.709549414843146	35.62296920378411	21.244500675733963
49	22.057681801190444	21.005549644880237	32.453633148345176	24.48313540558415
50	19.746671650803695	23.818615751789977	27.822986456566124	28.61172614084021
51	19.204071656554618	23.761106478419645	33.218506484170575	23.816315380855162
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4676.0
1	3681.5
2	2687.0
3	1866.0
4	1045.0
5	1221.5
6	1398.0
7	1296.5
8	1195.0
9	1134.5
10	1074.0
11	1172.0
12	1270.0
13	1252.5
14	1235.0
15	1302.0
16	1369.0
17	1389.5
18	1410.0
19	1378.0
20	1346.0
21	1366.5
22	1387.0
23	1518.5
24	1650.0
25	1870.5
26	2393.5
27	2696.0
28	3008.5
29	3321.0
30	3808.5
31	4296.0
32	5176.5
33	6057.0
34	6537.0
35	7017.0
36	7768.5
37	8520.0
38	9327.0
39	10134.0
40	14005.5
41	17877.0
42	25448.0
43	33019.0
44	36577.5
45	40136.0
46	42974.5
47	45813.0
48	41505.5
49	37198.0
50	32757.0
51	28316.0
52	24496.0
53	20676.0
54	18035.5
55	15395.0
56	13503.5
57	11612.0
58	10532.0
59	9452.0
60	8173.0
61	6894.0
62	5985.0
63	5076.0
64	4121.5
65	3167.0
66	2775.5
67	2384.0
68	1939.5
69	1495.0
70	1362.0
71	1229.0
72	1071.5
73	914.0
74	789.0
75	455.5
76	247.0
77	176.5
78	106.0
79	76.0
80	46.0
81	59.0
82	72.0
83	70.0
84	68.0
85	39.0
86	10.0
87	17.5
88	25.0
89	13.5
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	347770.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.012492413726775
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.02113751006272	30.828115276353817
2	9.44679970144093	7.370864033729213
3	3.2524381818898225	3.8065715969127596
4	1.6229454464864297	2.53260587675777
5	0.9521269865170814	1.8572423419201085
6	0.6605374338750353	1.5461526976819417
7	0.49654661174592635	1.356006464665978
8	0.3876820018107316	1.2099552923663661
9	0.34400748382718016	1.2078530417765792
>10	3.387447230655431	28.562755882504753
>50	0.3624911774222808	8.933504002201909
>100	0.05696289930921556	4.421152284348744
>500	0.0036988895655334776	1.0819831525571801
>1k	0.004438667478640173	3.6609646012770956
>5k	7.397779131066955E-4	1.6242734549458016
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCT	5628	1.6183109526411135	Illumina PCR Primer Index 5 (95% over 24bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4022	1.1565114874773557	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCC	1906	0.5480633752192541	No Hit
AAACTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCT	1845	0.5305230468413031	Illumina PCR Primer Index 5 (95% over 21bp)
AATCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCT	1795	0.5161457284987204	Illumina PCR Primer Index 5 (95% over 21bp)
AATGATACCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGT	1687	0.48509072087874167	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTT	1430	0.41119130459786646	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	968	0.27834488311240185	No Hit
CCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC	774	0.22256088794318082	Illumina PCR Primer Index 5 (95% over 23bp)
AAAAAACTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCT	750	0.21565977513874113	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	737	0.2119216723696696	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	520	0.1495241107628605	No Hit
AACTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTG	495	0.14233545159156913	Illumina PCR Primer Index 5 (95% over 22bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	475	0.13658452425453604	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCT	448	0.12882077234954137	No Hit
ACCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTG	434	0.1247951232136182	Illumina PCR Primer Index 5 (95% over 22bp)
AATGATCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCT	397	0.11415590764010695	No Hit
AATGACTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTT	392	0.1127181758058487	No Hit
TACCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCT	386	0.11099289760473877	Illumina PCR Primer Index 5 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC	379	0.10898007303677719	Illumina PCR Primer Index 5 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC	365	0.10495442390085401	Illumina PCR Primer Index 5 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.9199470914684993	0.0
2	0.0	0.0	0.0	2.5352963165310407	0.0
3	0.0	0.0	0.0	3.2719901084049803	0.0
4	0.0	0.0	0.0	6.6650372372545075	0.0
5	0.0	0.0	0.0	7.238117146389856	0.0
6	0.0	0.0	0.0	9.69433821203669	0.0
7	0.0	0.0	0.0	11.664605917704229	0.0
8	0.0	0.0	0.0	12.750093452569226	0.0
9	0.0	0.0	0.0	14.847456652385198	0.0
10	0.0	0.0	0.0	17.07766627368663	0.0
11	0.0	0.0	0.0	19.947379014866147	0.0
12	0.0	0.0	0.0	21.12028064525405	0.0
13	0.0	0.0	0.0	21.63211317824999	0.0
14	0.0	0.0	0.0	22.201167438249417	0.0
15	0.0	0.0	0.0	22.617247031083764	0.0
16	0.0	0.0	0.0	23.42467722920321	0.0
17	0.0	0.0	0.0	24.490899157489146	0.0
18	0.0	0.0	0.0	25.94473358829111	0.0
19	0.0	0.0	0.0	26.577048048997902	0.0
20	0.0	0.0	0.0	27.12453633148345	0.0
21	0.0	0.0	0.0	27.751962503953763	0.0
22	0.0	0.0	0.0	28.488943842194555	0.0
23	0.0	0.0	0.0	29.189694338212036	0.0
24	0.0	0.0	0.0	29.644017597837653	0.0
25	0.0	0.0	0.0	30.081950714552722	0.0
26	0.0	0.0	0.0	30.483940535411335	0.0
27	0.0	0.0	0.0	30.897719757310867	0.0
28	0.0	0.0	0.0	31.288207723495415	0.0
29	0.0	0.0	0.0	31.72211519107456	0.0
30	0.0	0.0	0.0	32.289731719239725	0.0
31	0.0	0.0	0.0	32.73571613422664	0.0
32	0.0	0.0	0.0	33.14288178968859	0.0
33	0.0	0.0	0.0	33.533944848606836	0.0
34	0.0	0.0	0.0	33.93881013313397	0.0
35	0.0	0.0	0.0	34.365816487908674	0.0
36	0.0	0.0	0.0	34.75745463956063	0.0
37	0.0	0.0	0.0	35.135290565603704	0.0
38	0.0	0.0	0.0	35.49788653420364	0.0
39	0.0	0.0	0.0	35.90994047790206	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTGATC	65	0.0	45.000004	16
CGGGTTA	25	3.8851755E-5	45.000004	6
GATCGGG	50	2.1827873E-11	45.000004	3
CGCGGTA	25	3.8851755E-5	45.000004	10
TCGGGCG	25	3.8851755E-5	45.000004	5
TAGCCCG	25	3.8851755E-5	45.000004	1
CGCTATA	25	3.8851755E-5	45.000004	33
TAACACG	25	3.8851755E-5	45.000004	1
TCGCTCG	25	3.8851755E-5	45.000004	21
ACGCTAT	25	3.8851755E-5	45.000004	32
CGTACCA	25	3.8851755E-5	45.000004	45
ATGCGAG	25	3.8851755E-5	45.000004	1
CGGTCCC	25	3.8851755E-5	45.000004	20
ATCGGGC	50	2.1827873E-11	45.000004	4
ATACCCG	25	3.8851755E-5	45.000004	1
ACGACGT	25	3.8851755E-5	45.000004	23
TCGTTTA	20	7.026493E-4	45.0	38
ACCGGTC	20	7.026493E-4	45.0	32
ACCGGGT	45	3.8380676E-10	45.0	4
ATAGGAC	20	7.026493E-4	45.0	16
>>END_MODULE