FastQCFastQC Report
Sat 18 Jun 2016
SRR3552826_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552826_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences303563
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCT38021.2524583035481927Illumina Single End Adapter 1 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30000.9882627329417616No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCC21310.7019959612996314No Hit
AATCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCT19900.6555476128513686No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGT19670.6479709318988152No Hit
AAACTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCT11730.3864107285802288No Hit
AAAAACTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTT9580.31558523271940253No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7540.24838336687936277No Hit
CCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC6080.20028791387619702No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5810.1913935492797212No Hit
AAAAAACTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCT5100.16800466460009947No Hit
AATGACTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTT4760.15680435362675954No Hit
AACTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTG4710.15515724907185657No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4290.1413215708106719No Hit
AATGATCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCT3850.1268270507275261No Hit
AACCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCT3470.11430905611026376No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3420.11266195155536084No Hit
ACCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTG3360.1106854260894773No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCACGGTGTTCGTAT3140.10343816604790439No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGTAG302.1602063E-645.0000041
CACGGGT302.1602063E-645.0000044
ATTACCG302.1602063E-645.0000041
CGATTGG302.1602063E-645.0000042
ATATAGG351.2079727E-745.0000042
TCTCGTA650.045.00000428
CCGACGG302.1602063E-645.0000042
CGTAACG302.1602063E-645.0000041
ATAACGG302.1602063E-645.0000042
TTTCTAC302.1602063E-645.00000440
ATTGACG351.2079727E-745.0000041
AGTACGG302.1602063E-645.0000042
TGCAACG302.1602063E-645.0000041
TATCCGG302.1602063E-645.0000042
CGGAAAG207.0250774E-445.01
TCGTTGC207.0250774E-445.016
GTACCGC207.0250774E-445.029
CTCGTTG207.0250774E-445.015
GCGAATG253.8840015E-545.01
CCGGCTA253.8840015E-545.041
Produced by FastQC (version 0.11.5)