##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552826_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	303563
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.013779676706317	31.0	31.0	33.0	28.0	34.0
2	31.28647101260694	31.0	31.0	34.0	28.0	34.0
3	31.534788495304106	31.0	31.0	34.0	30.0	34.0
4	35.500802139918235	37.0	35.0	37.0	33.0	37.0
5	34.76514924414372	35.0	35.0	37.0	32.0	37.0
6	34.86000270125147	35.0	35.0	37.0	32.0	37.0
7	34.88990423734118	35.0	35.0	37.0	32.0	37.0
8	35.27236850340786	37.0	35.0	37.0	33.0	37.0
9	36.824280297664735	39.0	37.0	39.0	33.0	39.0
10	36.066786795492206	37.0	35.0	39.0	31.0	39.0
11	36.289103085685674	37.0	35.0	39.0	32.0	39.0
12	36.048250280831326	37.0	35.0	39.0	32.0	39.0
13	35.79830216462481	37.0	35.0	39.0	30.0	39.0
14	36.81613042432708	38.0	35.0	40.0	31.0	41.0
15	36.785576634833625	38.0	35.0	40.0	32.0	41.0
16	36.655979154244754	38.0	35.0	40.0	31.0	41.0
17	36.62259234491687	38.0	35.0	40.0	31.0	41.0
18	36.47070295128194	38.0	35.0	40.0	31.0	41.0
19	36.57375240065489	38.0	35.0	40.0	31.0	41.0
20	36.39888919268817	38.0	35.0	40.0	30.0	41.0
21	36.511969508800476	38.0	35.0	40.0	31.0	41.0
22	36.51142267008825	38.0	35.0	40.0	31.0	41.0
23	36.38467797458847	38.0	35.0	40.0	31.0	41.0
24	35.92274420795683	38.0	34.0	40.0	29.0	41.0
25	36.14695466838844	38.0	34.0	40.0	30.0	41.0
26	35.90350932096467	38.0	34.0	40.0	30.0	41.0
27	35.83500624252626	38.0	34.0	40.0	29.0	41.0
28	35.85468584774824	38.0	34.0	40.0	29.0	41.0
29	35.495033979766966	38.0	34.0	40.0	27.0	41.0
30	35.54700342268327	38.0	34.0	40.0	28.0	41.0
31	35.36403975451554	38.0	34.0	40.0	27.0	41.0
32	35.3185401382909	38.0	34.0	40.0	27.0	41.0
33	35.17530792619654	38.0	34.0	40.0	27.0	41.0
34	35.185651742801326	38.0	34.0	40.0	27.0	41.0
35	34.9825308090907	38.0	34.0	40.0	25.0	41.0
36	34.967687102841914	37.0	33.0	40.0	26.0	41.0
37	34.955795007955516	37.0	33.0	40.0	26.0	41.0
38	34.896726544407585	37.0	33.0	40.0	26.0	41.0
39	34.86324749722463	37.0	33.0	40.0	26.0	41.0
40	34.53518709460639	37.0	33.0	40.0	24.0	41.0
41	34.728725174016596	37.0	33.0	40.0	25.0	41.0
42	34.71574928433307	37.0	33.0	40.0	25.0	41.0
43	34.75571792346235	37.0	33.0	40.0	25.0	41.0
44	34.65835098480381	37.0	33.0	40.0	25.0	41.0
45	34.51581385083162	37.0	33.0	40.0	24.0	41.0
46	34.438762299753265	37.0	33.0	40.0	24.0	41.0
47	34.3184742541087	37.0	33.0	40.0	24.0	41.0
48	34.348253904461345	37.0	33.0	40.0	24.0	41.0
49	34.24508256935134	37.0	33.0	40.0	24.0	41.0
50	34.1958539084144	37.0	33.0	40.0	24.0	41.0
51	32.87927382454384	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	2.0
11	6.0
12	3.0
13	13.0
14	18.0
15	35.0
16	68.0
17	143.0
18	301.0
19	566.0
20	927.0
21	1263.0
22	1782.0
23	2131.0
24	2440.0
25	2674.0
26	3251.0
27	3798.0
28	4626.0
29	5866.0
30	7718.0
31	10268.0
32	13720.0
33	19293.0
34	29376.0
35	33780.0
36	37736.0
37	47849.0
38	49878.0
39	24028.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.687389438106752	36.27879550538109	20.781847590121323	24.25196746639083
2	19.925682642482776	36.93928443189717	20.756482179975823	22.378550745644233
3	19.899987811426293	33.774537740106666	22.1703567299045	24.15511771856254
4	19.760972186992486	33.66088752581836	19.63480397808692	26.94333630910223
5	16.816278663737016	36.95509663562424	19.463505104377017	26.765119596261734
6	20.67050332220989	36.050836234982526	21.570481251008854	21.708179191798738
7	74.35688802653814	10.24367264785234	8.907212012004099	6.4922273136054125
8	76.40226246281662	7.675836646758663	6.92245102334606	8.999449867078663
9	71.14569298629938	10.946656871884914	12.345048638997506	5.562601502818196
10	34.1863138788324	36.71461937060841	13.512845768423688	15.586220982135504
11	24.469714688549	26.026228492932272	31.30453974957422	18.199517068944502
12	26.203786363950808	24.381429884406202	31.761775974015276	17.653007777627707
13	21.835006242526266	32.36099261108897	26.230798878651218	19.57320226773355
14	16.028962686493415	33.14699090468865	32.100091249592346	18.723955159225596
15	14.430941847326583	32.30499105622226	31.635937186020698	21.628129910430456
16	16.130753747986414	30.41543271083762	31.413578071108798	22.040235470067167
17	16.36266606931675	29.522372621169247	29.2278703267526	24.887090982761404
18	17.898096935397266	29.133985367123135	27.532011477024536	25.435906220455063
19	19.4552695816025	34.03510968069231	27.939834564818504	18.569786172886683
20	21.11423328930074	32.808346208200604	26.143502337241365	19.933918165257296
21	18.076313648237765	32.69403715209034	26.272305913434774	22.957343286237123
22	17.32819875940085	31.474191518729228	27.568906619054363	23.62870310281556
23	19.8354213128741	30.91615249552811	23.62870310281556	25.61972308878223
24	15.931124675932178	33.04454100137369	27.586695348247314	23.43763897444682
25	17.634230785701817	30.484611102143543	26.694294100400906	25.18686401175374
26	19.34128994640322	36.0462243422288	22.91748335600847	21.695002355359513
27	15.474547293313085	32.19990578561946	25.882930396655716	26.44261652441174
28	19.208862740189023	28.62634774330205	27.72340502630426	24.44138449020467
29	19.99354335014478	31.8971679684283	25.79299848795802	22.3162901934689
30	19.534330600237844	29.139256101698823	24.471361793103902	26.85505150495943
31	25.818363898103524	32.00324150176405	19.620968299825737	22.55742630030669
32	24.339922849622646	29.35601506112405	21.671613470679894	24.63244861857341
33	22.274124316863386	27.406172689029955	25.57920431673162	24.74049867737504
34	23.379990315025218	28.13056927227627	22.997203216465774	25.492237196232743
35	20.813801418486445	26.448216679898408	27.80608967496039	24.931892226654764
36	24.192671702414327	26.800038212825672	27.984965229622844	21.022324855137153
37	22.76463205331348	28.20963029091161	25.15326307883372	23.872474576941194
38	25.203335057302766	26.592503038907907	28.66719593626364	19.53696596752569
39	24.91311523472887	25.123944617756443	29.20546970480592	20.75747044270876
40	24.472679476747825	26.166891221920984	27.07543409440544	22.284995206925746
41	21.847194816232545	23.278858095354177	31.666573330741887	23.20737375767139
42	24.4815738413443	25.78212759789566	26.388262074099938	23.3480364866601
43	20.327905574790076	26.198845050286103	28.738680273946432	24.734569100977392
44	22.660205624532633	25.258348349436528	25.803869378020377	26.277576648010463
45	25.57854547490966	27.314264254866373	25.23726541113377	21.869924859090204
46	20.58320678080003	25.892483603074158	31.295315964066766	22.228993652059046
47	21.925267572134942	25.901048546759654	26.71340051323778	25.460283367867625
48	22.756067109627985	22.981391012738705	33.005998754788955	21.256543122844352
49	22.498130536330187	21.645259797801444	30.465504689306666	25.391104976561703
50	20.69422162780049	23.39184946782052	27.06719857163093	28.84673033274806
51	19.353807941020477	24.01412556866285	32.761897859752345	23.870168630564333
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3496.0
1	2800.5
2	2105.0
3	1421.0
4	737.0
5	905.0
6	1073.0
7	1020.5
8	968.0
9	968.5
10	969.0
11	1023.0
12	1077.0
13	1115.5
14	1154.0
15	1167.5
16	1181.0
17	1150.5
18	1120.0
19	1143.0
20	1166.0
21	1208.5
22	1251.0
23	1262.5
24	1274.0
25	1554.0
26	1945.5
27	2057.0
28	2308.5
29	2560.0
30	3137.0
31	3714.0
32	4374.5
33	5035.0
34	5923.5
35	6812.0
36	7789.5
37	8767.0
38	9542.5
39	10318.0
40	11564.0
41	12810.0
42	15020.5
43	17231.0
44	19509.5
45	21788.0
46	26459.5
47	31131.0
48	32787.5
49	34444.0
50	35696.5
51	36949.0
52	33409.0
53	29869.0
54	25261.0
55	20653.0
56	17399.5
57	14146.0
58	11521.0
59	8896.0
60	7546.5
61	6197.0
62	5130.5
63	4064.0
64	3422.5
65	2781.0
66	2173.0
67	1565.0
68	1176.0
69	787.0
70	626.5
71	466.0
72	475.5
73	485.0
74	366.0
75	192.5
76	138.0
77	134.0
78	130.0
79	100.5
80	71.0
81	44.0
82	17.0
83	17.5
84	18.0
85	11.0
86	4.0
87	4.0
88	4.0
89	2.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	303563.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.35831809931182
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.84823773290752	33.39878735460658
2	9.320025241187412	7.895611877196637
3	3.317092108145814	4.2151932804467185
4	1.6298440567978392	2.761498120404629
5	1.0513702510590013	2.226713776725526
6	0.7594345227257621	1.9301021453530136
7	0.5760398255329625	1.7080054717458117
8	0.4855822586898211	1.6454758221572614
9	0.40295477753037184	1.5361637981642027
>10	3.402097854474374	29.285274735379502
>50	0.16133655558822735	4.341040805833337
>100	0.03741137520886431	3.2872266562995316
>500	0.0038970182509233658	1.1261153082894146
>1k	0.004676421901108039	4.642790847397841
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCT	3802	1.2524583035481927	Illumina Single End Adapter 1 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3000	0.9882627329417616	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCC	2131	0.7019959612996314	No Hit
AATCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCT	1990	0.6555476128513686	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGT	1967	0.6479709318988152	No Hit
AAACTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCT	1173	0.3864107285802288	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTT	958	0.31558523271940253	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	754	0.24838336687936277	No Hit
CCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC	608	0.20028791387619702	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	581	0.1913935492797212	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCT	510	0.16800466460009947	No Hit
AATGACTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTT	476	0.15680435362675954	No Hit
AACTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTG	471	0.15515724907185657	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	429	0.1413215708106719	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCT	385	0.1268270507275261	No Hit
AACCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCT	347	0.11430905611026376	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	342	0.11266195155536084	No Hit
ACCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTG	336	0.1106854260894773	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCACGGTGTTCGTAT	314	0.10343816604790439	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.588418219611744E-4	0.0	0.0	1.5008416704275553	0.0
2	6.588418219611744E-4	0.0	0.0	2.0410919644357186	0.0
3	6.588418219611744E-4	0.0	0.0	2.7216755665216117	0.0
4	6.588418219611744E-4	0.0	0.0	6.036638193719261	0.0
5	6.588418219611744E-4	0.0	0.0	6.540322766608579	0.0
6	6.588418219611744E-4	0.0	0.0	8.999449867078662	0.0
7	6.588418219611744E-4	0.0	0.0	10.915691306252738	0.0
8	6.588418219611744E-4	0.0	0.0	12.093700483919319	0.0
9	6.588418219611744E-4	0.0	0.0	14.368681295151253	0.0
10	6.588418219611744E-4	0.0	0.0	16.724370229573434	0.0
11	6.588418219611744E-4	0.0	0.0	19.787984701692896	0.0
12	6.588418219611744E-4	0.0	0.0	21.123127653897214	0.0
13	9.882627329417617E-4	0.0	0.0	21.684790307119115	0.0
14	9.882627329417617E-4	0.0	0.0	22.30508988249556	0.0
15	9.882627329417617E-4	0.0	0.0	22.722795597618944	0.0
16	9.882627329417617E-4	0.0	0.0	23.49989952662215	0.0
17	9.882627329417617E-4	0.0	0.0	24.57117632913102	0.0
18	9.882627329417617E-4	0.0	0.0	25.93893195152242	0.0
19	9.882627329417617E-4	0.0	0.0	26.592503038907903	0.0
20	9.882627329417617E-4	0.0	0.0	27.181837048652174	0.0
21	9.882627329417617E-4	0.0	0.0	27.849902656120804	0.0
22	9.882627329417617E-4	0.0	0.0	28.595052756758893	0.0
23	9.882627329417617E-4	0.0	0.0	29.304295978100097	0.0
24	9.882627329417617E-4	0.0	0.0	29.82741638473727	0.0
25	9.882627329417617E-4	0.0	0.0	30.273122877294004	0.0
26	9.882627329417617E-4	0.0	0.0	30.695111064260136	0.0
27	9.882627329417617E-4	0.0	0.0	31.13982929408393	0.0
28	9.882627329417617E-4	0.0	0.0	31.60595988312146	0.0
29	9.882627329417617E-4	0.0	0.0	32.05990189845271	0.0
30	9.882627329417617E-4	0.0	0.0	32.71017877672839	0.0
31	0.0013176836439223489	0.0	0.0	33.16939152663533	0.0
32	0.0013176836439223489	0.0	0.0	33.58709724175871	0.0
33	0.0013176836439223489	0.0	0.0	34.031156629760545	0.0
34	0.0013176836439223489	0.0	0.0	34.43930913846549	0.0
35	0.0013176836439223489	0.0	0.0	34.90313378112616	0.0
36	0.0013176836439223489	0.0	0.0	35.33698112088759	0.0
37	0.0013176836439223489	0.0	0.0	35.781699350711385	0.0
38	0.0013176836439223489	0.0	0.0	36.1717337093124	0.0
39	0.0013176836439223489	0.0	0.0	36.59075710807971	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAGTAG	30	2.1602063E-6	45.000004	1
CACGGGT	30	2.1602063E-6	45.000004	4
ATTACCG	30	2.1602063E-6	45.000004	1
CGATTGG	30	2.1602063E-6	45.000004	2
ATATAGG	35	1.2079727E-7	45.000004	2
TCTCGTA	65	0.0	45.000004	28
CCGACGG	30	2.1602063E-6	45.000004	2
CGTAACG	30	2.1602063E-6	45.000004	1
ATAACGG	30	2.1602063E-6	45.000004	2
TTTCTAC	30	2.1602063E-6	45.000004	40
ATTGACG	35	1.2079727E-7	45.000004	1
AGTACGG	30	2.1602063E-6	45.000004	2
TGCAACG	30	2.1602063E-6	45.000004	1
TATCCGG	30	2.1602063E-6	45.000004	2
CGGAAAG	20	7.0250774E-4	45.0	1
TCGTTGC	20	7.0250774E-4	45.0	16
GTACCGC	20	7.0250774E-4	45.0	29
CTCGTTG	20	7.0250774E-4	45.0	15
GCGAATG	25	3.8840015E-5	45.0	1
CCGGCTA	25	3.8840015E-5	45.0	41
>>END_MODULE