FastQCFastQC Report
Sat 18 Jun 2016
SRR3552824_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552824_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences317234
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCT54551.7195508678136642TruSeq Adapter, Index 14 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39191.235365692201971No Hit
AAACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCT20760.6544065264126796No Hit
AAAAACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTT16540.5213816930089461No Hit
AATCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCT14300.45077135489890746No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCC13570.4277599500684038No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGT12460.39277000573709No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9830.30986590340253567No Hit
CCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC9100.286854498572032TruSeq Adapter, Index 15 (95% over 21bp)
AAAAAACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCT8830.2783434310319827No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7410.23358152026579748No Hit
GAAAAACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCT5600.1765258452750966No Hit
GCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC4930.15540578878682612TruSeq Adapter, Index 15 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG4880.1538296651682985TruSeq Adapter, Index 14 (95% over 21bp)
AACCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCT4590.14468814818083814No Hit
AACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG4540.14311202456231048No Hit
TCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC4520.14248157511489942TruSeq Adapter, Index 15 (95% over 21bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4180.13176393450891138No Hit
GAAAACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTT3970.12514421531109526No Hit
GAACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCT3910.1232528669688621No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3770.11883972083698469No Hit
TAAAACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTT3680.11600269832363491No Hit
CAAAAACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCT3580.11285045108657962No Hit
AATGACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTT3420.10780685550729115No Hit
AATGATCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCT3390.10686118133617455No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTG207.025557E-445.010
TCTTGTC207.025557E-445.024
CCTTACG207.025557E-445.01
CTCACCC207.025557E-445.014
TCGCAGG502.1827873E-1145.02
CTCGTCC207.025557E-445.037
ATTAGCG207.025557E-445.01
ACGCATG253.8843988E-545.01
ACTTGCG207.025557E-445.01
CCGGCCC207.025557E-445.019
TTTCGAG207.025557E-445.01
GGCCGTA253.8843988E-545.042
GACCGAA207.025557E-445.09
CCGGACA207.025557E-445.024
TAAACGG351.2082091E-745.02
TGAACGG253.8843988E-545.02
TTCAACG253.8843988E-545.01
TACGGGT253.8843988E-545.04
CGACACG406.7884685E-945.01
CATTCGG253.8843988E-545.02
Produced by FastQC (version 0.11.5)