##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552824_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	317234
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.98983085041326	31.0	31.0	33.0	28.0	34.0
2	31.26430332183814	31.0	31.0	34.0	28.0	34.0
3	31.516019720458715	31.0	31.0	34.0	30.0	34.0
4	35.49384996564051	37.0	35.0	37.0	33.0	37.0
5	34.743479576590154	35.0	35.0	37.0	32.0	37.0
6	34.84305276231425	35.0	35.0	37.0	32.0	37.0
7	34.828322310975494	35.0	35.0	37.0	32.0	37.0
8	35.2054666271585	36.0	35.0	37.0	32.0	37.0
9	36.780023578809335	39.0	37.0	39.0	32.0	39.0
10	36.078585523619786	37.0	35.0	39.0	31.0	39.0
11	36.31402686975545	37.0	35.0	39.0	32.0	39.0
12	36.067281565027706	37.0	35.0	39.0	32.0	39.0
13	35.79008870423725	37.0	35.0	39.0	30.0	39.0
14	36.78765201712301	38.0	35.0	40.0	31.0	41.0
15	36.729237723573135	38.0	35.0	40.0	31.0	41.0
16	36.6411891537477	38.0	35.0	40.0	31.0	41.0
17	36.58587036698462	38.0	35.0	40.0	31.0	41.0
18	36.44255975084638	38.0	35.0	40.0	31.0	41.0
19	36.49417464710592	38.0	35.0	40.0	31.0	41.0
20	36.3129424967059	38.0	34.0	40.0	30.0	41.0
21	36.40298013453791	38.0	35.0	40.0	31.0	41.0
22	36.42059489209858	38.0	35.0	40.0	30.0	41.0
23	36.24465851705681	38.0	35.0	40.0	30.0	41.0
24	35.75133182445766	38.0	34.0	40.0	29.0	41.0
25	35.89144290965029	38.0	34.0	40.0	30.0	41.0
26	35.72889097637706	38.0	34.0	40.0	29.0	41.0
27	35.631666214844564	38.0	34.0	40.0	29.0	41.0
28	35.45243574144007	38.0	34.0	40.0	27.0	41.0
29	35.29665483523204	38.0	34.0	40.0	27.0	41.0
30	35.20487400467793	37.0	34.0	40.0	27.0	41.0
31	35.07733408146668	37.0	34.0	40.0	27.0	41.0
32	35.10403992005901	38.0	34.0	40.0	26.0	41.0
33	34.96885264505066	38.0	34.0	40.0	25.0	41.0
34	34.83901788585082	37.0	34.0	40.0	25.0	41.0
35	34.77609272650473	37.0	34.0	40.0	25.0	41.0
36	34.804507083099544	37.0	33.0	40.0	25.0	41.0
37	34.76271143698342	37.0	33.0	40.0	25.0	41.0
38	34.74902753172737	37.0	33.0	40.0	25.0	41.0
39	34.73009828706885	37.0	33.0	40.0	25.0	41.0
40	34.38893687309683	37.0	33.0	40.0	24.0	41.0
41	34.444050763789505	37.0	33.0	40.0	24.0	41.0
42	34.341328483075586	37.0	33.0	40.0	23.0	41.0
43	34.40374297836928	37.0	33.0	40.0	24.0	41.0
44	34.28714450531784	37.0	33.0	40.0	23.0	41.0
45	34.10372154308807	37.0	33.0	40.0	23.0	41.0
46	34.027717709955425	37.0	33.0	40.0	23.0	41.0
47	33.90195250193863	37.0	33.0	40.0	23.0	41.0
48	33.90961876721915	37.0	33.0	40.0	23.0	41.0
49	33.812904669739055	37.0	33.0	40.0	22.0	41.0
50	33.74735368844449	37.0	33.0	40.0	22.0	41.0
51	32.47065888271749	35.0	31.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	4.0
11	4.0
12	5.0
13	9.0
14	19.0
15	40.0
16	66.0
17	151.0
18	332.0
19	648.0
20	1192.0
21	1731.0
22	2322.0
23	2760.0
24	3206.0
25	3344.0
26	3742.0
27	4318.0
28	5157.0
29	6434.0
30	8253.0
31	10822.0
32	14369.0
33	20169.0
34	30471.0
35	34587.0
36	38928.0
37	49365.0
38	50943.0
39	23840.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.037997188195465	35.87099743407075	19.59689062332537	25.49411475440842
2	19.820069727708884	37.116765542155	20.133087878348473	22.93007685178764
3	19.270633034290146	36.93866357326138	21.1093388476645	22.68136454478398
4	18.393677852941362	36.115611819666235	18.772893195559114	26.717817131833282
5	15.545307249538196	37.39542419791069	20.590163727721492	26.46910482482962
6	19.771840344981936	37.58739605464736	20.677480976187923	21.96328262418278
7	70.926823732639	11.247533366537004	10.25079279018012	7.574850110643879
8	73.66612658164004	9.220323168386743	8.231778434846202	8.881771815127005
9	68.06931161224837	12.023931861023723	14.270538466873035	5.636218059854871
10	31.468253718075616	36.63888486101742	14.248787960937353	17.64407345996961
11	23.235214384334594	26.45870240894734	32.11919277252753	18.186890434190534
12	24.27829299507619	25.433591607456957	31.88056765668245	18.407547740784405
13	20.49307451282019	32.84988368207695	26.937843988979743	19.719197816123113
14	14.755669316655846	33.885712124173324	32.82592660307533	18.5326919560955
15	13.008378673156093	33.462995769684206	32.06686546839242	21.46176008876728
16	13.848137337107625	30.532351513393895	32.529615362792136	23.089895786706343
17	13.360799914258875	31.258314052087734	30.283323981666534	25.09756205198686
18	16.357641362527346	29.44640234022835	27.63259928002673	26.563357017217577
19	17.076984182023363	35.311473549493435	28.242243895673226	19.369298372809975
20	20.556749907008705	32.32377361821243	26.897495224345434	20.221981250433434
21	17.035374518494233	34.18549083641728	25.847481669682317	22.931652975406166
22	15.619700284332701	31.62239860796762	28.150828725798622	24.60707238190106
23	18.800317746521493	32.09775749131556	22.941740166564745	26.160184595598203
24	13.974227226589836	34.461627694383324	27.522585851453503	24.041559227573337
25	16.10924428024739	32.367274630083784	26.875114268962342	24.64836682070648
26	19.453463374039355	34.54264044837564	23.22134449649155	22.78255168109345
27	14.15232919548346	32.522680418870614	24.96453721858313	28.3604531670628
28	17.24373806086359	32.24906535869421	28.5029347421777	22.0042618382645
29	16.784140413700925	30.154397069670967	24.67169345026069	28.389769066367414
30	19.011833536127906	31.80302237465089	22.95245780717073	26.232686282050473
31	19.57293354432375	31.57227787689844	23.899708101905848	24.955080476871963
32	19.45377859876306	27.770667709009757	22.548970160827654	30.226583531399537
33	21.11280631962526	28.224276086422012	25.608856553837228	25.0540610401155
34	14.437607570436963	28.14736125383786	28.30749541348027	29.107535762244908
35	18.341350548806243	26.87700561730458	27.60013113348506	27.18151270040412
36	19.772786019153056	25.143900086371573	31.20630197267632	23.877011921799053
37	16.96035103425232	27.141479160493514	28.74975570083913	27.148414104415036
38	20.444845130093242	26.21881639420743	30.7605742133567	22.575764262342624
39	20.4650195124104	23.836347932441036	30.772552752857514	24.926079802291053
40	23.694181582049843	25.914624535831592	25.984289199770515	24.406904682348046
41	18.647118530800608	22.364878922183625	34.22962229773606	24.758380249279714
42	22.36740071997327	25.74156616251726	27.82961473234269	24.061418385166785
43	18.13992195035841	24.823631767086756	30.22815965501806	26.80828662753677
44	20.443269006474715	23.228909889860482	26.980399326679986	29.34742177698481
45	23.426240566900145	25.15493295170127	27.527944671756494	23.890881809642096
46	18.36373150418934	24.211780578374324	32.20997749295473	25.214510424481613
47	20.182578159970245	24.73946676585738	27.330298769993128	27.747656304179248
48	19.984301808759465	21.165448848484083	36.49009879142841	22.360150551328044
49	20.642806256580315	19.873657930738826	32.39848187773063	27.085053934950228
50	19.03547539040582	20.8470718775415	29.035979749963747	31.081472982088933
51	18.12100846693608	21.57744756236721	34.74123202431013	25.560311946386577
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4538.0
1	3636.5
2	2735.0
3	1869.0
4	1003.0
5	1117.5
6	1232.0
7	1175.5
8	1119.0
9	1177.0
10	1235.0
11	1268.5
12	1302.0
13	1365.0
14	1428.0
15	1510.5
16	1593.0
17	1610.0
18	1627.0
19	1578.5
20	1530.0
21	1533.5
22	1537.0
23	1634.5
24	1732.0
25	1965.0
26	2302.5
27	2407.0
28	3111.5
29	3816.0
30	3900.0
31	3984.0
32	4781.0
33	5578.0
34	6100.5
35	6623.0
36	6626.0
37	6629.0
38	7468.5
39	8308.0
40	10046.0
41	11784.0
42	16082.5
43	20381.0
44	26314.0
45	32247.0
46	34826.5
47	37406.0
48	39859.5
49	42313.0
50	38238.0
51	34163.0
52	29422.5
53	24682.0
54	20727.5
55	16773.0
56	13947.5
57	11122.0
58	9205.0
59	7288.0
60	6379.0
61	5470.0
62	4862.5
63	4255.0
64	3197.5
65	2140.0
66	1736.5
67	1333.0
68	1188.5
69	1044.0
70	871.0
71	698.0
72	734.5
73	771.0
74	613.5
75	403.5
76	351.0
77	282.5
78	214.0
79	156.0
80	98.0
81	62.5
82	27.0
83	35.5
84	44.0
85	26.0
86	8.0
87	5.5
88	3.0
89	2.5
90	2.0
91	3.0
92	4.0
93	3.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	317234.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.62050120635629
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.04265074903046	32.89794740853622
2	9.311152074064571	7.750696322623428
3	3.239524468327964	4.044918961261942
4	1.691442671303705	2.8159476696591343
5	1.0132426249548956	2.108583294713342
6	0.7174065593842721	1.7915292342180609
7	0.5404586133337813	1.5745910857771002
8	0.4320046652802101	1.4384200553957223
9	0.38499917763704816	1.4421472863560048
>10	3.441442786075524	29.53261929795856
>50	0.1295939976077403	3.566194807859523
>100	0.04698729737824502	4.344979079980978
>500	0.003789298175664921	1.285987637682936
>1k	0.004547157810797905	3.684794599806735
>5k	7.578596351329842E-4	1.7206432581703253
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCT	5455	1.7195508678136642	TruSeq Adapter, Index 14 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3919	1.235365692201971	No Hit
AAACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCT	2076	0.6544065264126796	No Hit
AAAAACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTT	1654	0.5213816930089461	No Hit
AATCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCT	1430	0.45077135489890746	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCC	1357	0.4277599500684038	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGT	1246	0.39277000573709	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	983	0.30986590340253567	No Hit
CCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	910	0.286854498572032	TruSeq Adapter, Index 15 (95% over 21bp)
AAAAAACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCT	883	0.2783434310319827	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	741	0.23358152026579748	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCT	560	0.1765258452750966	No Hit
GCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	493	0.15540578878682612	TruSeq Adapter, Index 15 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG	488	0.1538296651682985	TruSeq Adapter, Index 14 (95% over 21bp)
AACCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCT	459	0.14468814818083814	No Hit
AACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG	454	0.14311202456231048	No Hit
TCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	452	0.14248157511489942	TruSeq Adapter, Index 15 (95% over 21bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	418	0.13176393450891138	No Hit
GAAAACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTT	397	0.12514421531109526	No Hit
GAACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCT	391	0.1232528669688621	No Hit
GGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	377	0.11883972083698469	No Hit
TAAAACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTT	368	0.11600269832363491	No Hit
CAAAAACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCT	358	0.11285045108657962	No Hit
AATGACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTT	342	0.10780685550729115	No Hit
AATGATCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCT	339	0.10686118133617455	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.041710535440716	0.0
2	0.0	0.0	0.0	2.8562512214958042	0.0
3	0.0	0.0	0.0	3.6972707843421575	0.0
4	0.0	0.0	0.0	7.534816570733276	0.0
5	0.0	0.0	0.0	8.164005119249513	0.0
6	0.0	0.0	0.0	11.349981401741301	0.0
7	0.0	0.0	0.0	13.890377450084165	0.0
8	0.0	0.0	0.0	15.328117414905085	0.0
9	0.0	0.0	0.0	17.63367104408733	0.0
10	0.0	0.0	0.0	20.348386364639353	0.0
11	0.0	0.0	0.0	23.566200344225397	0.0
12	0.0	0.0	0.0	25.113323288172136	0.0
13	0.0	0.0	0.0	25.739674814175025	0.0
14	0.0	0.0	0.0	26.36697201434903	0.0
15	0.0	0.0	0.0	26.86817932504082	0.0
16	0.0	0.0	0.0	27.789581192432085	0.0
17	0.0	0.0	0.0	29.05426278393867	0.0
18	0.0	0.0	0.0	30.675148313232505	0.0
19	0.0	0.0	0.0	31.417502537559027	0.0
20	0.0	0.0	0.0	32.05898485029978	0.0
21	3.1522472370552966E-4	0.0	0.0	32.76256643361052	0.0
22	3.1522472370552966E-4	0.0	0.0	33.53581268086018	0.0
23	3.1522472370552966E-4	0.0	0.0	34.291721568306045	0.0
24	3.1522472370552966E-4	0.0	0.0	34.783156912562966	0.0
25	3.1522472370552966E-4	0.0	0.0	35.19326427810386	0.0
26	3.1522472370552966E-4	0.0	0.0	35.56649035097121	0.0
27	3.1522472370552966E-4	0.0	0.0	36.009696312501184	0.0
28	3.1522472370552966E-4	0.0	0.0	36.44029328508294	0.0
29	3.1522472370552966E-4	0.0	0.0	36.886966718573674	0.0
30	3.1522472370552966E-4	0.0	0.0	37.45846914265179	0.0
31	3.1522472370552966E-4	0.0	0.0	37.895055384983955	0.0
32	6.304494474110593E-4	0.0	0.0	38.296336458261095	0.0
33	6.304494474110593E-4	0.0	0.0	38.70266112711752	0.0
34	6.304494474110593E-4	0.0	0.0	39.102366076776136	0.0
35	6.304494474110593E-4	0.0	0.0	39.52918035267342	0.0
36	6.304494474110593E-4	0.0	0.0	39.918167661726045	0.0
37	6.304494474110593E-4	0.0	0.0	40.2750020489607	0.0
38	6.304494474110593E-4	0.0	0.0	40.63246688564278	0.0
39	6.304494474110593E-4	0.0	0.0	41.006953857404945	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTG	20	7.025557E-4	45.0	10
TCTTGTC	20	7.025557E-4	45.0	24
CCTTACG	20	7.025557E-4	45.0	1
CTCACCC	20	7.025557E-4	45.0	14
TCGCAGG	50	2.1827873E-11	45.0	2
CTCGTCC	20	7.025557E-4	45.0	37
ATTAGCG	20	7.025557E-4	45.0	1
ACGCATG	25	3.8843988E-5	45.0	1
ACTTGCG	20	7.025557E-4	45.0	1
CCGGCCC	20	7.025557E-4	45.0	19
TTTCGAG	20	7.025557E-4	45.0	1
GGCCGTA	25	3.8843988E-5	45.0	42
GACCGAA	20	7.025557E-4	45.0	9
CCGGACA	20	7.025557E-4	45.0	24
TAAACGG	35	1.2082091E-7	45.0	2
TGAACGG	25	3.8843988E-5	45.0	2
TTCAACG	25	3.8843988E-5	45.0	1
TACGGGT	25	3.8843988E-5	45.0	4
CGACACG	40	6.7884685E-9	45.0	1
CATTCGG	25	3.8843988E-5	45.0	2
>>END_MODULE