##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552821_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1158992
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.802405883733453	31.0	31.0	34.0	30.0	34.0
2	31.975646941480182	33.0	31.0	34.0	30.0	34.0
3	32.01580770186507	33.0	31.0	34.0	30.0	34.0
4	35.63025111476179	37.0	35.0	37.0	33.0	37.0
5	35.50020017394426	37.0	35.0	37.0	33.0	37.0
6	35.46592556290294	37.0	35.0	37.0	33.0	37.0
7	35.80007713599404	37.0	35.0	37.0	35.0	37.0
8	35.771116625481454	37.0	35.0	37.0	35.0	37.0
9	37.37066433590569	39.0	37.0	39.0	35.0	39.0
10	36.852970512307245	39.0	37.0	39.0	32.0	39.0
11	36.67088383698938	39.0	35.0	39.0	32.0	39.0
12	36.46328188632881	38.0	35.0	39.0	32.0	39.0
13	36.370488320885734	38.0	35.0	39.0	32.0	39.0
14	37.407485987823904	39.0	36.0	41.0	32.0	41.0
15	37.510091527810374	39.0	36.0	41.0	32.0	41.0
16	37.58813520714552	39.0	36.0	41.0	33.0	41.0
17	37.57481156039041	39.0	36.0	41.0	33.0	41.0
18	37.522497998260555	39.0	36.0	41.0	32.0	41.0
19	37.48040711238732	39.0	36.0	41.0	32.0	41.0
20	37.422410163314325	39.0	35.0	41.0	32.0	41.0
21	37.33278573104905	39.0	35.0	41.0	32.0	41.0
22	37.29962760743819	39.0	35.0	41.0	32.0	41.0
23	37.25613205268026	39.0	35.0	41.0	32.0	41.0
24	37.225869548711294	39.0	35.0	41.0	32.0	41.0
25	37.15461021301269	39.0	35.0	41.0	32.0	41.0
26	37.04763967309524	39.0	35.0	41.0	31.0	41.0
27	36.97405158965722	39.0	35.0	41.0	31.0	41.0
28	36.8771095917832	39.0	35.0	40.0	31.0	41.0
29	36.85461590761627	39.0	35.0	40.0	31.0	41.0
30	36.74039855322556	39.0	35.0	40.0	31.0	41.0
31	36.595602903212445	39.0	35.0	40.0	30.0	41.0
32	36.48423802752737	38.0	35.0	40.0	30.0	41.0
33	36.38978526167566	38.0	35.0	40.0	30.0	41.0
34	36.24592404434198	38.0	35.0	40.0	30.0	41.0
35	36.18400817261897	38.0	35.0	40.0	30.0	41.0
36	36.09392040669823	38.0	35.0	40.0	30.0	41.0
37	36.039206482874775	38.0	35.0	40.0	30.0	41.0
38	35.96698682993498	38.0	35.0	40.0	29.0	41.0
39	35.93716781479078	38.0	35.0	40.0	29.0	41.0
40	35.79110899816392	38.0	34.0	40.0	29.0	41.0
41	35.66295884699808	38.0	34.0	40.0	28.0	41.0
42	35.560537087400085	38.0	34.0	40.0	28.0	41.0
43	35.49819757168298	38.0	34.0	40.0	28.0	41.0
44	35.37416824274887	38.0	34.0	40.0	28.0	41.0
45	35.29336095503679	38.0	34.0	40.0	27.0	41.0
46	35.18766738683269	37.0	34.0	40.0	27.0	41.0
47	35.05949393956127	37.0	34.0	40.0	27.0	41.0
48	34.98625788616315	37.0	34.0	40.0	26.0	41.0
49	34.962516566119525	37.0	34.0	40.0	27.0	41.0
50	34.8427055579331	37.0	34.0	40.0	26.0	41.0
51	33.35635621298508	35.0	31.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	7.0
10	8.0
11	3.0
12	10.0
13	12.0
14	29.0
15	62.0
16	138.0
17	243.0
18	477.0
19	832.0
20	1419.0
21	2182.0
22	3405.0
23	4868.0
24	6482.0
25	8508.0
26	10871.0
27	13514.0
28	16941.0
29	21082.0
30	26163.0
31	33149.0
32	42198.0
33	56692.0
34	90428.0
35	104431.0
36	105902.0
37	145976.0
38	216739.0
39	246181.0
40	36.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.990029266811156	20.522833634744675	25.814328312878775	10.672808785565389
2	30.486232864420117	23.645547165122796	26.564721758217484	19.3034982122396
3	31.97071248119055	24.87609923105595	26.241509863743666	16.91167842400983
4	28.581991937821833	26.532970029128762	25.859626295953724	19.02541173709568
5	25.843319022046746	30.18165785441142	23.799215180087526	20.175807943454313
6	27.299411902756876	35.606026616232036	22.386694645001864	14.707866836009224
7	82.18857420931293	6.043441197178238	7.208073912503278	4.559910681005563
8	83.0192960779712	4.751974129243342	7.066916769054489	5.161813023730966
9	77.68500559106533	6.958546737164709	9.30800212598534	6.048445545784613
10	43.918163369548715	25.033649930284245	15.945148887999228	15.103037812167816
11	33.100055910653396	25.71277454891837	21.162182310145365	20.024987230282868
12	29.81426964120546	22.946405152063175	27.449628642820656	19.78969656391071
13	25.776105443350772	24.344861741927467	28.60640970774604	21.272623106975715
14	20.70808081505308	28.47077460413877	27.707870287284127	23.11327429352403
15	20.375119068984084	25.850566699338735	32.50824854701327	21.266065684663914
16	22.620432237668595	25.094047241050845	30.107369162168506	22.178151359112057
17	22.898346149067468	24.81535679279926	26.848157709457876	25.4381393486754
18	24.167552493891243	24.61820271408258	28.607445090216327	22.606799701809848
19	25.09421980479589	27.368264837030797	25.218293137484988	22.319222220688324
20	27.74402239187156	26.161957977276806	25.189216146444497	20.90480348440714
21	25.450218810828716	26.966622720432927	26.26713557988321	21.316022888855144
22	23.37168850173254	25.67412027002775	25.023382387453925	25.930808840785787
23	23.72958570896089	26.604411419578394	26.00017946629485	23.665823405165867
24	24.09757789527451	24.500341676215193	26.591382856827313	24.81069757168298
25	22.751666965777158	26.251432279083893	25.323988431326534	25.67291232381242
26	22.486781617129367	28.54238855833345	25.746079351712524	23.224750472824663
27	23.435019396164943	28.31037660311719	25.269113160401453	22.985490840316412
28	20.600487320016015	28.359125861093087	26.35212322431906	24.688263594571836
29	23.211031655093393	26.816319698496628	25.073425873517678	24.89922277289231
30	23.734762621312314	26.544100390684317	24.635200242969756	25.085936745033617
31	25.192408575727875	27.921159076162734	22.193336968676228	24.693095379433164
32	24.677392078633847	28.949035023537697	22.42077598464873	23.95279691317973
33	24.265913828568273	27.17922125433135	23.092825489735908	25.46203942736447
34	22.49221737509836	26.756785206455262	25.304575010008694	25.446422408437673
35	21.43500559106534	28.67224277648163	23.953228322542348	25.939523309910683
36	22.73009649764623	30.092442398221902	24.215784060631997	22.96167704349987
37	24.112677222966163	28.747221723704737	24.1619441721772	22.978156881151897
38	22.46866242389939	28.529014868092272	23.62475323384458	25.377569474163757
39	23.91241699683863	24.10586095503679	25.080846114554717	26.90087593356986
40	23.396278835401798	24.938222179273023	26.576283529135665	25.089215456189518
41	20.71645015668788	27.50778262490164	25.43649999309745	26.33926722531303
42	22.548559437856344	26.28534105498571	26.23417590457915	24.93192360257879
43	23.642182172094373	24.99439167828596	26.604583983323437	24.75884216629623
44	23.326304236785067	24.860395930256637	25.122865386473762	26.690434446484527
45	22.57030246973232	24.37402501484048	24.880758452172234	28.17491406325497
46	22.60360727252647	25.996555627648853	25.10215773706807	26.297679362756604
47	20.973483854936013	26.361355384679104	27.738672915775087	24.9264878446098
48	21.627414166793212	24.617081049739774	27.52892168366995	26.22658309979706
49	22.656929469746125	23.536486878252827	28.67819622568577	25.128387426315278
50	20.70454325827961	24.057111697060897	27.280171045184094	27.958173999475406
51	20.50997763573864	23.934764001822273	25.280070958211965	30.275187404227122
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	405.0
1	769.0
2	1133.0
3	1409.5
4	1686.0
5	1401.0
6	1116.0
7	1151.0
8	1186.0
9	1294.0
10	1402.0
11	1455.5
12	1509.0
13	1660.0
14	1811.0
15	2023.0
16	2235.0
17	2337.0
18	2439.0
19	2819.5
20	3200.0
21	3482.5
22	3765.0
23	3959.5
24	4154.0
25	5069.0
26	7247.0
27	8510.0
28	9784.0
29	11058.0
30	13612.5
31	16167.0
32	17817.5
33	19468.0
34	21378.5
35	23289.0
36	26447.0
37	29605.0
38	32831.0
39	36057.0
40	41590.5
41	47124.0
42	52031.5
43	56939.0
44	61118.0
45	65297.0
46	76852.0
47	88407.0
48	99941.0
49	111475.0
50	110767.5
51	110060.0
52	98037.5
53	86015.0
54	78133.5
55	70252.0
56	66881.5
57	63511.0
58	62045.0
59	60579.0
60	58260.0
61	55941.0
62	52201.5
63	48462.0
64	41297.5
65	34133.0
66	30245.5
67	26358.0
68	22594.0
69	18830.0
70	16072.0
71	13314.0
72	11284.5
73	9255.0
74	7818.5
75	5176.5
76	3971.0
77	3467.5
78	2964.0
79	2460.0
80	1956.0
81	1324.5
82	693.0
83	544.0
84	395.0
85	352.0
86	309.0
87	214.0
88	119.0
89	79.0
90	39.0
91	28.0
92	17.0
93	14.5
94	12.0
95	7.5
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1158992.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.2418911644756
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.06074824729292	16.05102394500831
2	12.49269484715706	5.807077079772591
3	5.057578610578068	3.5264307486850557
4	2.591379092201322	2.409142033073628
5	1.5106385497612713	1.7555048381206366
6	0.9558123878568593	1.3328932495335999
7	0.7022311301067269	1.1424825648773076
8	0.5070032871748161	0.9426972176438756
9	0.3756944296825793	0.7858664141204017
>10	4.674316701465699	28.701155569011664
>50	1.9179602071049973	30.06900737693264
>100	0.14650567340436865	5.316833624267835
>500	0.005205785349393458	0.7705480826704687
>1k	0.0022310508640257676	1.389337256282016
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTTCTGC	4387	0.3785185747615169	No Hit
CTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTTCTGCT	3662	0.31596421718182693	No Hit
CCTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTTCTGC	3300	0.28473017932824385	No Hit
TCTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTTCTGC	2229	0.19232229385535016	No Hit
ACTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTTCTGC	1442	0.12441846017919019	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3361541753523752	0.0
2	0.0	0.0	0.0	1.3830984165550755	0.0
3	0.0	0.0	0.0	1.8756816267929373	0.0
4	0.0	0.0	0.0	2.4256422822590666	0.0
5	0.0	0.0	0.0	3.8526581719287103	0.0
6	0.0	0.0	0.0	4.580877176028825	0.0
7	0.0	0.0	0.0	5.4046964948852105	0.0
8	0.0	0.0	0.0	6.73671604290625	0.0
9	0.0	0.0	0.0	7.169419633612656	0.0
10	0.0	0.0	0.0	8.351222441569917	0.0
11	0.0	0.0	0.0	10.125091458784874	0.0
12	0.0	0.0	0.0	11.376868865358864	0.0
13	0.0	0.0	0.0	11.865310545715587	0.0
14	0.0	0.0	0.0	12.05840937642365	0.0
15	0.0	0.0	0.0	12.448403440230821	0.0
16	0.0	0.0	0.0	13.26462995430512	0.0
17	8.628187252371026E-5	0.0	0.0	14.242462415616329	0.0
18	8.628187252371026E-5	0.0	0.0	15.339622706627829	0.0
19	1.7256374504742052E-4	0.0	0.0	15.941179921863137	0.0
20	2.588456175711308E-4	0.0	0.0	16.493901597250023	0.0
21	4.314093626185513E-4	0.0	0.0	17.22652097684885	0.0
22	7.765368527133923E-4	0.0	0.0	17.98347184449936	0.0
23	0.0012942280878556539	0.0	0.0	18.715228405372944	0.0
24	0.0017256374504742052	0.0	0.0	19.302894239131934	0.0
25	0.003968966136090672	0.0	0.0	19.821793420489527	0.0
26	0.004227811753661803	0.0	0.0	20.283401438491378	0.0
27	0.004572939243756644	0.0	0.0	20.743715228405375	0.0
28	0.004572939243756644	0.0	0.0	21.204891837044606	0.0
29	0.004572939243756644	0.0	0.0	21.701444013418556	0.0
30	0.004572939243756644	0.0	0.0	22.225692670872622	0.0
31	0.004572939243756644	0.0	0.0	22.740450322349076	0.0
32	0.005004348606375195	0.0	0.0	23.22492303656971	0.0
33	0.0051769123514226155	0.0	0.0	23.69636718803926	0.0
34	0.005349476096470036	0.0	0.0	24.178941701064375	8.628187252371026E-5
35	0.005349476096470036	0.0	0.0	24.68722821210155	8.628187252371026E-5
36	0.005349476096470036	0.0	0.0	25.175238483095654	8.628187252371026E-5
37	0.005522039841517457	0.0	0.0	25.66247221723705	1.7256374504742052E-4
38	0.005522039841517457	0.0	0.0	26.15695362867043	1.7256374504742052E-4
39	0.005608321714041167	0.0	0.0	26.669467951461268	1.7256374504742052E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTACGG	50	2.1827873E-11	45.0	2
TCCGATC	20	7.033312E-4	45.0	15
ACCGAGT	20	7.033312E-4	45.0	17
CGTTACG	25	3.8908256E-5	45.0	20
TACGCGC	20	7.033312E-4	45.0	13
TCCACGT	25	3.8908256E-5	45.0	23
CGAATCC	20	7.033312E-4	45.0	44
CGTCGCA	20	7.033312E-4	45.0	32
ACCCGTT	20	7.033312E-4	45.0	42
CCAACCG	20	7.033312E-4	45.0	2
GTAACGT	20	7.033312E-4	45.0	9
ATACCCG	20	7.033312E-4	45.0	26
CGATCAA	65	0.0	41.53846	10
CGACAAT	40	3.4582627E-7	39.375	20
TATACGG	80	0.0	39.375	2
ACGCGAT	35	6.248334E-6	38.571426	12
TACGACG	35	6.248334E-6	38.571426	1
TTACGCG	70	0.0	38.571426	1
CGTAAGG	150	0.0	37.500004	2
CGAGTTA	30	1.1399279E-4	37.499996	40
>>END_MODULE