FastQCFastQC Report
Sat 18 Jun 2016
SRR3552820_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552820_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences518288
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTTCTGC21380.4125119624610255No Hit
GCTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTTCTGC17900.34536782638224306No Hit
CTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTTCTGCT16720.32260056184978236No Hit
TCTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTTCTGC7870.15184607785632698No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT253.88783E-545.00000437
CGGTCGT207.029696E-445.00000437
GCGTACG207.029696E-445.0000041
GTAGTAG502.1827873E-1145.0000041
CCGTAAG302.1631859E-644.9999961
GCTACGA302.1631859E-644.99999610
CGCAAGG850.042.352942
CGACCAA850.039.70588329
CACGACC850.039.70588327
GGCGATA850.039.7058838
CGGGTAC403.453315E-739.3750046
TTAATCG403.453315E-739.37500420
GGCCGAT800.039.3750048
ATAGCGG403.453315E-739.3750042
TCGATGG403.453315E-739.3750042
GGCCTAT403.453315E-739.3750048
ACGCATT356.241651E-638.57142617
TACGAAT356.241651E-638.57142612
CAAGGCG356.241651E-638.5714261
CGAATAT356.241651E-638.57142614
Produced by FastQC (version 0.11.5)