##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552820_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	518288
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.96124355570648	33.0	33.0	33.0	27.0	33.0
2	32.097748742012165	33.0	33.0	33.0	33.0	33.0
3	32.14401066588461	33.0	33.0	33.0	33.0	33.0
4	32.089477279041766	33.0	33.0	33.0	33.0	33.0
5	32.1432716945019	33.0	33.0	33.0	33.0	33.0
6	35.57816889451425	37.0	37.0	37.0	33.0	37.0
7	35.75197187664001	37.0	37.0	37.0	33.0	37.0
8	35.76932709227302	37.0	37.0	37.0	33.0	37.0
9	35.64642824066928	37.0	37.0	37.0	33.0	37.0
10	35.702574630321365	37.0	37.0	37.0	33.0	37.0
11	35.66860317043806	37.0	37.0	37.0	33.0	37.0
12	35.66844109838545	37.0	37.0	37.0	33.0	37.0
13	35.69636765659247	37.0	37.0	37.0	33.0	37.0
14	35.75929984873275	37.0	37.0	37.0	33.0	37.0
15	35.80822824375637	37.0	37.0	37.0	33.0	37.0
16	35.8249834069089	37.0	37.0	37.0	33.0	37.0
17	35.83044369153829	37.0	37.0	37.0	33.0	37.0
18	35.81231863365542	37.0	37.0	37.0	33.0	37.0
19	35.79154446948415	37.0	37.0	37.0	33.0	37.0
20	35.76956827092273	37.0	37.0	37.0	33.0	37.0
21	35.65547919303553	37.0	37.0	37.0	33.0	37.0
22	35.72591686475473	37.0	37.0	37.0	33.0	37.0
23	35.74851819837619	37.0	37.0	37.0	33.0	37.0
24	35.76083760380329	37.0	37.0	37.0	33.0	37.0
25	35.75670283703269	37.0	37.0	37.0	33.0	37.0
26	35.70150186768746	37.0	37.0	37.0	33.0	37.0
27	35.70466227271324	37.0	37.0	37.0	33.0	37.0
28	35.67963757601951	37.0	37.0	37.0	33.0	37.0
29	35.66907202173309	37.0	37.0	37.0	33.0	37.0
30	35.659141249652706	37.0	37.0	37.0	33.0	37.0
31	35.64613110857284	37.0	37.0	37.0	33.0	37.0
32	35.617224400333406	37.0	37.0	37.0	33.0	37.0
33	35.59616661007008	37.0	37.0	37.0	33.0	37.0
34	35.56983954866792	37.0	37.0	37.0	33.0	37.0
35	35.541743200691506	37.0	37.0	37.0	33.0	37.0
36	35.5338942827154	37.0	37.0	37.0	33.0	37.0
37	35.52584470410274	37.0	37.0	37.0	33.0	37.0
38	35.5086805019603	37.0	37.0	37.0	33.0	37.0
39	35.50953330966567	37.0	37.0	37.0	33.0	37.0
40	35.490289182848144	37.0	37.0	37.0	33.0	37.0
41	35.499986493995614	37.0	37.0	37.0	33.0	37.0
42	35.48260233692464	37.0	37.0	37.0	33.0	37.0
43	35.464849658876915	37.0	37.0	37.0	33.0	37.0
44	35.41200838144044	37.0	37.0	37.0	33.0	37.0
45	35.20713387151545	37.0	37.0	37.0	33.0	37.0
46	35.35195875652147	37.0	37.0	37.0	33.0	37.0
47	35.36815052634829	37.0	37.0	37.0	33.0	37.0
48	35.39024827894916	37.0	37.0	37.0	33.0	37.0
49	35.396920245114686	37.0	37.0	37.0	33.0	37.0
50	35.398579554224675	37.0	37.0	37.0	33.0	37.0
51	35.07279350476955	37.0	37.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	347.0
15	1358.0
16	977.0
17	675.0
18	534.0
19	514.0
20	544.0
21	629.0
22	798.0
23	1089.0
24	1504.0
25	2175.0
26	2967.0
27	4257.0
28	5574.0
29	6783.0
30	8249.0
31	10268.0
32	13378.0
33	18296.0
34	27875.0
35	58122.0
36	351375.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.33710215169944	19.326320501342884	23.831923563732904	13.504653783224772
2	29.361088815484827	23.54578921371901	26.385523106844072	20.70759886395209
3	32.5185996974655	24.59173278177384	25.367170376315872	17.522497144444788
4	29.28661284845491	26.05096008396876	25.250632852776832	19.411794214799492
5	26.120998363844038	29.537438644151514	23.810699842558577	20.530863149445867
6	27.14803352576174	35.649677399438154	21.94127589293983	15.261013181860278
7	81.33894668601242	6.508736455407033	7.354598215663878	4.79771864291668
8	82.62182415954065	5.119161547247862	6.96504646065508	5.293967832556416
9	77.41834655635476	7.2612138424968355	9.29598987435557	6.024449726792826
10	43.99175747846757	24.91143919982712	15.887305899422715	15.209497422282592
11	33.22129781125552	25.93480844626925	21.018429907696106	19.825463834779118
12	30.015744142252952	23.057643626709474	27.148612354521042	19.77799987651653
13	26.05636248572222	24.11554965578983	28.326142993856696	21.50194486463125
14	20.98119192418115	28.07956194239496	27.72551168462322	23.213734448800665
15	20.51639243046337	25.51882351125243	32.31813200382799	21.646652054456208
16	22.62043497051832	24.638810854196894	30.211002377056772	22.529751798228013
17	23.295349303861947	24.18963973698021	27.044616120766836	25.47039483839101
18	24.502014324082364	24.14584169419319	28.588545364739293	22.76359861698515
19	25.303306269873122	26.7864584941191	25.488145587009537	22.422089648998238
20	28.02958972617541	25.80052017411169	25.314496959219586	20.855393140493316
21	25.65041058253326	26.695582378909023	26.178495353934494	21.47551168462322
22	23.62122989534776	25.513806995338502	25.235197419195504	25.629765690118234
23	23.586307226870005	26.327833173833852	26.05539777112339	24.030461828172754
24	23.789090235544716	24.422135955298984	26.833729509461925	24.95504429969438
25	22.85273824591733	25.72122063408761	25.543520204982556	25.882520915012503
26	22.488462013397957	28.270768375883677	25.829847497916212	23.410922112802147
27	23.308855308245608	27.987335226746517	25.45013583181552	23.253673633192356
28	20.682130398542895	27.798444108295	26.79494798258883	24.724477510573273
29	23.30422467817121	26.327833173833852	25.516315253295463	24.85162689469947
30	23.53980798320625	26.12119130676381	25.1254128978483	25.213587812181643
31	25.03936035563239	27.687116043589665	22.616769055042756	24.65675454573519
32	24.669488778439785	28.592597166054396	22.796977742104776	23.940936313401043
33	24.339556385638872	26.97014015373692	23.48443336523323	25.20587009539098
34	22.426141450313338	26.68149754576606	25.624749174204304	25.267611829716298
35	21.690643040163	28.34948908714846	24.272026363720556	25.687841508967985
36	23.100862840737197	29.805436359707343	24.24559318371253	22.84810761584293
37	24.385669743463094	28.42068502454234	24.119408514185164	23.074236717809406
38	22.479393696168927	28.597613681968326	23.670623282808016	25.252369339054738
39	23.766515913932025	24.141596949958323	25.043605099867257	27.048282036242398
40	23.42577871762418	24.996912913283733	26.510936004692372	25.066372364399715
41	20.61286389034668	27.44053499212793	25.67066958910876	26.275931528416635
42	22.61483962584509	26.250077177167906	26.14530917173463	24.98977402525237
43	23.717315469391533	24.971830333714074	26.60856512209428	24.70228907480011
44	23.39876670885685	25.008682431389495	25.14412836106566	26.44842249868799
45	22.445821628129533	24.845838607106472	25.033186182199856	27.675153582564132
46	22.336422992621863	26.17463649553916	25.345367826382244	26.143572685456736
47	20.984857839656716	26.580781341647885	27.69367610286173	24.74068471583367
48	21.63179544963418	24.758628407371962	27.651228660513073	25.958347482480782
49	22.617926712561356	23.65943259346155	28.39907541752848	25.32356527644862
50	20.66862439415923	24.309650233075047	27.022041799154138	27.999683573611584
51	20.532599635723766	24.34823881702837	25.20702775290958	29.91213379433828
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	161.0
1	252.5
2	344.0
3	759.0
4	1174.0
5	947.0
6	720.0
7	702.5
8	685.0
9	726.0
10	767.0
11	773.0
12	779.0
13	840.0
14	901.0
15	1022.5
16	1144.0
17	1095.0
18	1046.0
19	1262.0
20	1478.0
21	1576.0
22	1674.0
23	1780.5
24	1887.0
25	2306.0
26	3116.0
27	3507.0
28	4101.0
29	4695.0
30	5715.5
31	6736.0
32	7659.5
33	8583.0
34	9218.5
35	9854.0
36	11414.0
37	12974.0
38	14516.0
39	16058.0
40	18279.0
41	20500.0
42	22654.5
43	24809.0
44	26364.0
45	27919.0
46	32865.0
47	37811.0
48	43097.5
49	48384.0
50	48948.5
51	49513.0
52	44267.0
53	39021.0
54	35541.5
55	32062.0
56	30563.5
57	29065.0
58	28289.5
59	27514.0
60	26549.5
61	25585.0
62	24130.5
63	22676.0
64	19372.0
65	16068.0
66	14339.0
67	12610.0
68	10649.5
69	8689.0
70	7425.0
71	6161.0
72	5329.5
73	4498.0
74	3828.5
75	2422.5
76	1686.0
77	1463.5
78	1241.0
79	1062.0
80	883.0
81	558.0
82	233.0
83	211.0
84	189.0
85	137.0
86	85.0
87	59.0
88	33.0
89	17.0
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	518288.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.140951004586135
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.60266163666302	20.865696545519558
2	10.72011017769776	6.247884109041111
3	3.7314297715214697	3.262122364468835
4	1.7638443876321308	2.056004115188086
5	1.1089460409805252	1.6157871123471625
6	0.7775171047673396	1.3594552713151622
7	0.6153533179807621	1.255238662285083
8	0.516589120845842	1.2043118608056724
9	0.4663850860623201	1.2231814447990583
>10	8.523861169230406	54.9106534667209
>50	0.12748666601815165	2.393132431631436
>100	0.041831562287206005	2.175645299261309
>500	0.0019919791565336195	0.34732153256617304
>1k	0.0019919791565336195	1.0835657840504562
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTTCTGC	2138	0.4125119624610255	No Hit
GCTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTTCTGC	1790	0.34536782638224306	No Hit
CTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTTCTGCT	1672	0.32260056184978236	No Hit
TCTGTCTCTTATACACATCTGACGCAAGCCCATTCGTATGCCGTCTTCTGC	787	0.15184607785632698	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.33938659586947795	0.0
2	0.0	0.0	0.0	1.3953631957521686	0.0
3	0.0	0.0	0.0	1.8514802580804495	0.0
4	0.0	0.0	0.0	2.332872842898157	0.0
5	0.0	0.0	0.0	3.505772852159417	0.0
6	0.0	0.0	0.0	4.122418423733523	0.0
7	0.0	0.0	0.0	4.830133053437471	0.0
8	0.0	0.0	0.0	6.003418948538265	0.0
9	0.0	0.0	0.0	6.426156885746921	0.0
10	0.0	0.0	0.0	7.617579415305776	0.0
11	0.0	0.0	0.0	9.372781156422684	0.0
12	0.0	0.0	0.0	10.618034760596425	0.0
13	0.0	0.0	0.0	11.127789954619825	0.0
14	0.0	0.0	0.0	11.313786929274844	0.0
15	0.0	0.0	0.0	11.675554903837249	0.0
16	0.0	0.0	0.0	12.452921927576945	0.0
17	0.0	0.0	0.0	13.375960855740438	0.0
18	0.0	0.0	0.0	14.423447967153397	0.0
19	1.9294291976661623E-4	0.0	0.0	15.015589787917143	0.0
20	1.9294291976661623E-4	0.0	0.0	15.588244373784459	0.0
21	1.9294291976661623E-4	0.0	0.0	16.338985274596364	0.0
22	3.8588583953323247E-4	0.0	0.0	17.096093291760564	0.0
23	9.647145988330812E-4	0.0	0.0	17.819822183805144	0.0
24	0.0015435433581329299	0.0	0.0	18.38958262587596	0.0
25	0.002315315037199395	0.0	0.0	18.893356589386595	0.0
26	0.002894143796499244	0.0	0.0	19.343878307041646	0.0
27	0.0030870867162658597	0.0	0.0	19.78841879418393	0.0
28	0.0030870867162658597	0.0	0.0	20.21945327694255	0.0
29	0.0030870867162658597	0.0	0.0	20.691777544531227	0.0
30	0.0030870867162658597	0.0	0.0	21.214652857098756	0.0
31	0.0030870867162658597	0.0	0.0	21.687941839286264	0.0
32	0.0034729725557990925	0.0	0.0	22.169334424103972	0.0
33	0.003665915475565709	0.0	0.0	22.645710493007748	0.0
34	0.003665915475565709	0.0	0.0	23.0887074367919	0.0
35	0.003665915475565709	0.0	0.0	23.586886055629304	0.0
36	0.003665915475565709	0.0	0.0	24.05573735066218	0.0
37	0.0038588583953323248	0.0	0.0	24.510503812552095	0.0
38	0.0038588583953323248	0.0	0.0	24.97781156422684	0.0
39	0.0038588583953323248	0.0	0.0	25.453608804371314	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGACAT	25	3.88783E-5	45.000004	37
CGGTCGT	20	7.029696E-4	45.000004	37
GCGTACG	20	7.029696E-4	45.000004	1
GTAGTAG	50	2.1827873E-11	45.000004	1
CCGTAAG	30	2.1631859E-6	44.999996	1
GCTACGA	30	2.1631859E-6	44.999996	10
CGCAAGG	85	0.0	42.35294	2
CGACCAA	85	0.0	39.705883	29
CACGACC	85	0.0	39.705883	27
GGCGATA	85	0.0	39.705883	8
CGGGTAC	40	3.453315E-7	39.375004	6
TTAATCG	40	3.453315E-7	39.375004	20
GGCCGAT	80	0.0	39.375004	8
ATAGCGG	40	3.453315E-7	39.375004	2
TCGATGG	40	3.453315E-7	39.375004	2
GGCCTAT	40	3.453315E-7	39.375004	8
ACGCATT	35	6.241651E-6	38.571426	17
TACGAAT	35	6.241651E-6	38.571426	12
CAAGGCG	35	6.241651E-6	38.571426	1
CGAATAT	35	6.241651E-6	38.571426	14
>>END_MODULE