FastQCFastQC Report
Sat 18 Jun 2016
SRR3552818_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552818_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1558763
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCTTCTGC62110.39845698159373816TruSeq Adapter, Index 20 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCTTCTGCT56450.3621461376745535TruSeq Adapter, Index 23 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCTTCTGC47040.30177775582304683TruSeq Adapter, Index 20 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26120.16756877087793334No Hit
TCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCTTCTGC24740.15871559691883885TruSeq Adapter, Index 20 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCC20880.13395237120716877No Hit
ACTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCTTCTGC16570.10630224094362002TruSeq Adapter, Index 23 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCG302.1660017E-645.0000041
CCGTAGT207.0340605E-445.029
GCGATAT1200.039.3750049
CGTATGG1700.038.382352
CGTTAGG1950.038.0769232
CGTTTTT22900.037.6310041
TACGACT301.1401082E-437.50000426
TGTTACG601.5643309E-1037.5000041
GCGATCG301.1401082E-437.5000049
CCGTTGA301.1401082E-437.50000438
GGCGATA4100.036.768298
ACGACGG1600.036.56252
CGACCAA2300.036.19565629
TTACGGG3050.036.147543
ACACGCG9650.036.13989636
TTACACG9650.036.13989634
CGAAAGG2500.036.0000042
CGTTCAC250.002107338336.039
CATCGCG250.002107338336.01
CGCACGA250.002107338336.040
Produced by FastQC (version 0.11.5)