##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552818_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1558763
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.654297029118602	33.0	33.0	33.0	27.0	33.0
2	31.858339593639315	33.0	33.0	33.0	27.0	33.0
3	31.887115616678095	33.0	33.0	33.0	27.0	33.0
4	31.792147363005153	33.0	33.0	33.0	27.0	33.0
5	31.85831778147159	33.0	33.0	33.0	27.0	33.0
6	35.31997038677464	37.0	37.0	37.0	33.0	37.0
7	35.61832299073047	37.0	37.0	37.0	33.0	37.0
8	35.68770557166163	37.0	37.0	37.0	33.0	37.0
9	35.55901506515102	37.0	37.0	37.0	33.0	37.0
10	35.55884249241225	37.0	37.0	37.0	33.0	37.0
11	35.56042515764103	37.0	37.0	37.0	33.0	37.0
12	35.58173436243996	37.0	37.0	37.0	33.0	37.0
13	35.655573682464876	37.0	37.0	37.0	33.0	37.0
14	35.703992204074645	37.0	37.0	37.0	33.0	37.0
15	35.71847420037555	37.0	37.0	37.0	33.0	37.0
16	35.73642946361955	37.0	37.0	37.0	33.0	37.0
17	35.74782503818733	37.0	37.0	37.0	33.0	37.0
18	35.732417307826786	37.0	37.0	37.0	33.0	37.0
19	35.72893570093722	37.0	37.0	37.0	33.0	37.0
20	35.71506636993565	37.0	37.0	37.0	33.0	37.0
21	35.64023203014185	37.0	37.0	37.0	33.0	37.0
22	35.681108673993414	37.0	37.0	37.0	33.0	37.0
23	35.696775584229286	37.0	37.0	37.0	33.0	37.0
24	35.69578377213213	37.0	37.0	37.0	33.0	37.0
25	35.69260946019376	37.0	37.0	37.0	33.0	37.0
26	35.650089846884995	37.0	37.0	37.0	33.0	37.0
27	35.6489787093997	37.0	37.0	37.0	33.0	37.0
28	35.62782411437787	37.0	37.0	37.0	33.0	37.0
29	35.62004101970601	37.0	37.0	37.0	33.0	37.0
30	35.58875723891316	37.0	37.0	37.0	33.0	37.0
31	35.56286747889192	37.0	37.0	37.0	33.0	37.0
32	35.53190895601192	37.0	37.0	37.0	33.0	37.0
33	35.5021943682266	37.0	37.0	37.0	33.0	37.0
34	35.46091484080646	37.0	37.0	37.0	33.0	37.0
35	35.44213713053235	37.0	37.0	37.0	33.0	37.0
36	35.445306951730316	37.0	37.0	37.0	33.0	37.0
37	35.44278572175501	37.0	37.0	37.0	33.0	37.0
38	35.405663336889575	37.0	37.0	37.0	33.0	37.0
39	35.39794375411785	37.0	37.0	37.0	33.0	37.0
40	35.38983155232707	37.0	37.0	37.0	33.0	37.0
41	35.38174372884139	37.0	37.0	37.0	33.0	37.0
42	35.36910293611024	37.0	37.0	37.0	33.0	37.0
43	35.36167845913715	37.0	37.0	37.0	33.0	37.0
44	35.33701018050852	37.0	37.0	37.0	33.0	37.0
45	35.27963006563538	37.0	37.0	37.0	33.0	37.0
46	35.29602447581833	37.0	37.0	37.0	33.0	37.0
47	35.28042749282604	37.0	37.0	37.0	33.0	37.0
48	35.27100207023133	37.0	37.0	37.0	33.0	37.0
49	35.26270189887751	37.0	37.0	37.0	33.0	37.0
50	35.24736666189793	37.0	37.0	37.0	33.0	37.0
51	34.8448686554659	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	905.0
15	3796.0
16	3443.0
17	2822.0
18	2782.0
19	2776.0
20	2925.0
21	3287.0
22	3741.0
23	4467.0
24	5672.0
25	7304.0
26	9688.0
27	13263.0
28	17516.0
29	21256.0
30	24798.0
31	30396.0
32	38913.0
33	53642.0
34	82488.0
35	174674.0
36	1048209.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.03104448848221	19.643909946540944	24.222604719254946	13.102440845721897
2	30.12953219957107	23.424728454550177	26.44802320814646	19.99771613773229
3	32.57441958783984	24.32582759534323	26.33787176113367	16.761881055683254
4	28.87007197373815	26.91903772414408	25.757411485902605	18.453478816215167
5	25.350037176915286	30.27971538970324	24.95016881976285	19.420078613618617
6	26.46925799496139	35.44361779180029	23.07951882357998	15.007605389658337
7	82.62461965032529	5.912123908509504	7.229193918511025	4.234062522654181
8	84.2869634447315	4.468735786004672	6.605045154394864	4.63925561486897
9	78.31934681539143	7.364878432449321	9.153027111882949	5.162747640276296
10	39.30924714020028	32.48595200168339	15.248116615547072	12.956684242569269
11	25.927738854463446	26.0580344799049	28.3796831205257	19.63454354510596
12	24.33609214486102	24.150175491720034	31.392264250562786	20.121468112856157
13	23.597942727662897	24.861508773302933	32.57704987865378	18.963498620380392
14	21.19167570695481	27.290999337295023	30.213059971272095	21.304264984478078
15	20.578048106094386	26.550732856758852	32.56659286883253	20.304626168314233
16	22.624286052465962	27.09327845220858	29.792020980739213	20.490414514586245
17	23.176582969957586	26.08664691168574	28.945131492086993	21.791638626269677
18	23.0975459386706	25.033504131160413	31.303796664406324	20.56515326576266
19	22.809432864393113	26.999357824120796	29.204247213976725	20.98696209750937
20	23.61353201224304	27.393259911866014	29.177431078361494	19.81577699752945
21	23.22925293967075	27.609841906691397	29.97889993539749	19.182005218240363
22	22.083280139443907	25.567709780126936	28.79610306377557	23.55290701665359
23	20.360503809751705	27.175009927743986	29.348977362177575	23.115508900326734
24	20.91440456310549	26.284175336468724	30.000070568777936	22.80134953164785
25	20.21115461426785	28.552127552424583	27.701517164572167	23.5352006687354
26	21.296694879208705	27.599577357173604	28.86051311199971	22.243214651617983
27	21.915839675434945	26.46656355071297	29.851491214507913	21.766105559344172
28	19.626075291753782	26.989606502078896	30.048506411815012	23.335811794352317
29	21.983842315990305	25.52799880417998	28.58946485129555	23.898694028534166
30	22.722440807229834	26.375465673742575	29.08684642886699	21.815247090160593
31	22.50322852159052	27.81417059552992	27.44984324108283	22.232757641796734
32	22.691198084635058	27.45747749978669	26.683017238669382	23.16830717690887
33	21.530726608214334	26.99615015239648	27.45580951049005	24.017313728899133
34	21.490502404791492	25.71571175348658	28.96899656971586	23.824789272006072
35	20.990811303578543	26.592047668567957	28.154312105175705	24.26282892267779
36	22.197729866567272	27.8997512771345	28.406563409575412	21.495955446722817
37	21.898967322165074	26.906463650984787	29.076196958742283	22.118372068107853
38	20.39129745830508	29.203477372762887	26.668133641868586	23.73709152706345
39	20.95501368713525	26.71297689257443	27.72916729483571	24.602842125454607
40	21.639210065930484	26.149388970613234	30.115097676811676	22.096303286644602
41	19.95915992360609	27.374847876168474	27.75444374802327	24.91154845220216
42	22.137361484715765	25.501631742606158	28.04820232453554	24.312804448142533
43	22.35054334751338	24.16698369155542	28.850376869350892	24.632096091580312
44	21.881453434550345	24.845149647508954	27.45997948373165	25.81341743420905
45	22.591567800878003	24.176157632686945	27.968908679510612	25.26336588692444
46	22.123055268825347	25.380189291123795	29.015443656283864	23.481311783767
47	19.624535609326113	26.20731952195427	30.917849602537395	23.25029526618222
48	20.39655803993295	25.666826836408102	29.415119553132836	24.521495570526117
49	22.23044811815523	23.956303812702764	30.178481270084035	23.63476679905797
50	20.617630775172362	24.113287266890477	29.428463467505967	25.840618490431194
51	20.190882128970216	23.712905682262154	27.47467062022899	28.621541568538646
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	306.0
1	449.5
2	593.0
3	2688.0
4	4783.0
5	3630.5
6	2478.0
7	2459.0
8	2440.0
9	2530.0
10	2620.0
11	2715.5
12	2811.0
13	2993.0
14	3175.0
15	3099.0
16	3023.0
17	3185.5
18	3348.0
19	3613.0
20	3878.0
21	4255.5
22	4633.0
23	4993.5
24	5354.0
25	7629.0
26	11546.5
27	13189.0
28	17186.5
29	21184.0
30	23123.5
31	25063.0
32	29072.5
33	33082.0
34	38323.0
35	43564.0
36	48073.0
37	52582.0
38	56437.5
39	60293.0
40	66779.0
41	73265.0
42	81594.0
43	89923.0
44	100158.5
45	110394.0
46	125810.0
47	141226.0
48	160794.0
49	180362.0
50	177233.0
51	174104.0
52	150504.5
53	126905.0
54	110936.0
55	94967.0
56	81852.0
57	68737.0
58	60920.0
59	53103.0
60	48146.5
61	43190.0
62	38315.0
63	33440.0
64	28676.0
65	23912.0
66	20289.5
67	16667.0
68	13545.5
69	10424.0
70	8496.5
71	6569.0
72	5742.5
73	4916.0
74	4135.0
75	2687.5
76	2021.0
77	1813.5
78	1606.0
79	1174.0
80	742.0
81	522.5
82	303.0
83	243.0
84	183.0
85	147.0
86	111.0
87	58.0
88	5.0
89	18.0
90	31.0
91	15.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1558763.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.38879671314989
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.57407306022124	21.14272362732902
2	12.126392499821213	7.370129530818648
3	4.908791192360218	4.475167729558061
4	2.3900992823235825	2.9052896485910717
5	1.4423797606781739	2.191609266520541
6	0.9152513708299589	1.6688032709750036
7	0.6828461738797894	1.4525611495069588
8	0.532195173828331	1.2938216759350898
9	0.4523367237892433	1.237137187061119
>10	6.695661841756009	46.576184673215806
>50	0.2218511972050361	4.158537987399198
>100	0.053012124566020974	3.037430351911113
>500	0.0029805992656613115	0.6287325621272905
>1k	0.0017031995803778923	1.0948150253465743
>5k	4.257998950944731E-4	0.7670563137045848
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCTTCTGC	6211	0.39845698159373816	TruSeq Adapter, Index 20 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCTTCTGCT	5645	0.3621461376745535	TruSeq Adapter, Index 23 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCTTCTGC	4704	0.30177775582304683	TruSeq Adapter, Index 20 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2612	0.16756877087793334	No Hit
TCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCTTCTGC	2474	0.15871559691883885	TruSeq Adapter, Index 20 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCC	2088	0.13395237120716877	No Hit
ACTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCTTCTGC	1657	0.10630224094362002	TruSeq Adapter, Index 23 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.370678544461217	0.0
2	0.0	0.0	0.0	1.3643510912178438	0.0
3	0.0	0.0	0.0	1.834339152263686	0.0
4	0.0	0.0	0.0	2.350132765532669	0.0
5	0.0	0.0	0.0	3.6200500011868386	0.0
6	0.0	0.0	0.0	4.323107489721016	0.0
7	0.0	0.0	0.0	5.133750287888537	0.0
8	0.0	0.0	0.0	6.625830867168389	0.0
9	0.0	0.0	0.0	7.179025932742823	0.0
10	0.0	0.0	0.0	8.621066833123445	0.0
11	0.0	0.0	0.0	10.812997229213165	0.0
12	0.0	0.0	0.0	12.470657822901877	0.0
13	0.0	0.0	0.0	13.12444547375066	0.0
14	0.0	0.0	0.0	13.380225217047107	0.0
15	0.0	0.0	0.0	13.83686936371982	0.0
16	0.0	0.0	0.0	14.765811095079881	0.0
17	0.0	0.0	0.0	16.0155199988709	0.0
18	0.0	0.0	0.0	17.333039082913825	0.0
19	0.0	0.0	0.0	18.187242063097468	0.0
20	0.0	0.0	0.0	19.0336183242738	0.0
21	2.5661373794476773E-4	0.0	0.0	20.053016398259388	0.0
22	3.849206069171516E-4	0.0	0.0	21.162421740829107	0.0
23	4.490740414033435E-4	0.0	0.0	22.20991901911965	0.0
24	8.98148082806687E-4	0.0	0.0	23.041668297233127	0.0
25	0.001026454951779071	0.0	0.0	23.744276711725902	0.0
26	0.001026454951779071	0.0	0.0	24.413846107458287	0.0
27	0.001026454951779071	0.0	0.0	25.077449233783454	0.0
28	0.001026454951779071	0.0	0.0	25.766521273599643	0.0
29	0.001090608386265263	0.0	0.0	26.434935907511278	0.0
30	0.001090608386265263	0.0	0.0	27.262258598645207	0.0
31	0.001090608386265263	0.0	0.0	27.981803519842337	0.0
32	0.001090608386265263	0.0	0.0	28.678317358058923	0.0
33	0.0011547618207514549	0.0	0.0	29.342177098122036	0.0
34	0.0013472221242100306	0.0	0.0	29.974986575893833	0.0
35	0.0013472221242100306	0.0	0.0	30.649624092950628	0.0
36	0.0014755289931824146	0.0	0.0	31.30777417734447	0.0
37	0.0016038358621547984	0.0	0.0	31.945074395530302	0.0
38	0.0016038358621547984	0.0	0.0	32.58917487777167	0.0
39	0.0016679892966409903	0.0	0.0	33.2785035313258	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCG	30	2.1660017E-6	45.000004	1
CCGTAGT	20	7.0340605E-4	45.0	29
GCGATAT	120	0.0	39.375004	9
CGTATGG	170	0.0	38.38235	2
CGTTAGG	195	0.0	38.076923	2
CGTTTTT	2290	0.0	37.631004	1
TACGACT	30	1.1401082E-4	37.500004	26
TGTTACG	60	1.5643309E-10	37.500004	1
GCGATCG	30	1.1401082E-4	37.500004	9
CCGTTGA	30	1.1401082E-4	37.500004	38
GGCGATA	410	0.0	36.76829	8
ACGACGG	160	0.0	36.5625	2
CGACCAA	230	0.0	36.195656	29
TTACGGG	305	0.0	36.14754	3
ACACGCG	965	0.0	36.139896	36
TTACACG	965	0.0	36.139896	34
CGAAAGG	250	0.0	36.000004	2
CGTTCAC	25	0.0021073383	36.0	39
CATCGCG	25	0.0021073383	36.0	1
CGCACGA	25	0.0021073383	36.0	40
>>END_MODULE