FastQCFastQC Report
Sat 18 Jun 2016
SRR3552817_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552817_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences673807
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTCTGC40680.6037337100979954Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTCTGC37990.5638112990811909Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTCTGCT36830.5465956868955056Illumina Single End Adapter 1 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTCTGC18430.2735204591225677Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCC12860.19085583854130336No Hit
ACTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTCTGC10920.1620642112652436Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTCTG9960.14781680807709033No Hit
GCCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTCTG9170.1360923825368392No Hit
GGCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTCTG6870.10195797906522193No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT6790.10077069546620918No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAAT207.031206E-445.033
ACACGTG453.8380676E-1045.042
TAGCGGA207.031206E-445.026
CACTCGC253.8890816E-545.022
TAATTCG207.031206E-445.012
TGCGATG406.8084773E-945.01
TCGCTCA207.031206E-445.028
TCGTCCC750.045.038
AATTCCG207.031206E-445.018
TTACGCA207.031206E-445.01
CGCCGTT207.031206E-445.026
CCTATCG207.031206E-445.012
ATGCACG207.031206E-445.01
GACCTAG502.1827873E-1145.01
ACGTACG302.164159E-644.99999621
AAATGCG302.164159E-644.9999961
TACGAAT1200.043.12499612
TAGCGAG850.042.352941
AAGTACG700.041.7857131
AAGCTAC1250.041.3999988
Produced by FastQC (version 0.11.5)