##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552817_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	673807
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.647630552962497	31.0	31.0	34.0	30.0	34.0
2	31.818289213380094	31.0	31.0	34.0	30.0	34.0
3	31.850985519592406	33.0	31.0	34.0	30.0	34.0
4	35.48174032029943	37.0	35.0	37.0	33.0	37.0
5	35.3654844784931	37.0	35.0	37.0	33.0	37.0
6	35.33663645524609	37.0	35.0	37.0	33.0	37.0
7	35.72402928434997	37.0	35.0	37.0	35.0	37.0
8	35.69870007286953	37.0	35.0	37.0	35.0	37.0
9	37.293020108131856	39.0	37.0	39.0	34.0	39.0
10	36.773717102968654	39.0	37.0	39.0	32.0	39.0
11	36.59479791691093	39.0	35.0	39.0	32.0	39.0
12	36.41036825084928	38.0	35.0	39.0	32.0	39.0
13	36.30062169137453	38.0	35.0	39.0	32.0	39.0
14	37.31367142223218	39.0	36.0	41.0	32.0	41.0
15	37.431789815184466	39.0	36.0	41.0	32.0	41.0
16	37.52684077191243	39.0	36.0	41.0	33.0	41.0
17	37.524363801503995	39.0	36.0	41.0	32.0	41.0
18	37.448680408484925	39.0	36.0	41.0	32.0	41.0
19	37.41895676358364	39.0	36.0	41.0	32.0	41.0
20	37.363098038459086	39.0	35.0	41.0	32.0	41.0
21	37.30193809206494	39.0	35.0	41.0	32.0	41.0
22	37.2328322501844	39.0	35.0	40.0	32.0	41.0
23	37.18766501386896	39.0	35.0	40.0	32.0	41.0
24	37.15328721725954	39.0	35.0	41.0	32.0	41.0
25	37.121297344788644	39.0	35.0	40.0	32.0	41.0
26	37.01571369843293	39.0	35.0	40.0	31.0	41.0
27	36.90456317610236	39.0	35.0	40.0	31.0	41.0
28	36.87233287870266	39.0	35.0	40.0	31.0	41.0
29	36.840478059741145	39.0	35.0	40.0	31.0	41.0
30	36.732225993496655	39.0	35.0	40.0	31.0	41.0
31	36.605421136913094	39.0	35.0	40.0	30.0	41.0
32	36.48364145816235	38.0	35.0	40.0	30.0	41.0
33	36.35019523394681	38.0	35.0	40.0	30.0	41.0
34	36.26286458882143	38.0	35.0	40.0	30.0	41.0
35	36.2227299508613	38.0	35.0	40.0	30.0	41.0
36	36.14740274292193	38.0	35.0	40.0	30.0	41.0
37	36.04201054604657	38.0	35.0	40.0	30.0	41.0
38	35.97409644007854	38.0	35.0	40.0	29.0	41.0
39	35.944859581452846	38.0	35.0	40.0	29.0	41.0
40	35.791502611281864	38.0	35.0	40.0	29.0	41.0
41	35.702637402104756	38.0	34.0	40.0	28.0	41.0
42	35.610689707883715	38.0	34.0	40.0	28.0	41.0
43	35.4879112268053	38.0	34.0	40.0	27.0	41.0
44	35.38175323200857	38.0	34.0	40.0	27.0	41.0
45	35.28661471311518	38.0	34.0	40.0	27.0	41.0
46	35.114823680965024	38.0	34.0	40.0	26.0	41.0
47	35.00514687440172	37.0	34.0	40.0	26.0	41.0
48	34.946088419977826	37.0	33.0	40.0	26.0	41.0
49	34.89678498442432	37.0	33.0	40.0	26.0	41.0
50	34.77849295124568	37.0	33.0	40.0	26.0	41.0
51	33.066182156017966	35.0	31.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	4.0
10	4.0
11	8.0
12	5.0
13	8.0
14	16.0
15	35.0
16	77.0
17	150.0
18	307.0
19	518.0
20	888.0
21	1287.0
22	1910.0
23	2758.0
24	3892.0
25	5187.0
26	6457.0
27	8024.0
28	9887.0
29	12417.0
30	15516.0
31	19934.0
32	25513.0
33	34363.0
34	52597.0
35	59550.0
36	62053.0
37	84834.0
38	128437.0
39	137152.0
40	16.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.4375229108632	19.492970539041597	26.315398919868745	11.754107630226459
2	32.0369185835113	24.59190836545183	25.304426935309372	18.0667461157275
3	31.560372628957552	23.701445666192246	27.84343291179819	16.894748793052017
4	28.827542604929903	26.955047958836875	25.826089666625606	18.391319769607616
5	25.003302132509752	30.29265056611166	24.654982806649382	20.0490644947292
6	26.00492425872691	34.8157558470007	24.451512079868568	14.727807814403828
7	78.7188319503953	6.584526429674967	9.120564197166251	5.5760774227634915
8	78.19642716682968	5.844403516140378	9.238105273468515	6.7210640435614355
9	72.30275138133027	7.687067661808203	12.368823713615324	7.641357243246212
10	41.016789674194534	24.20663483757218	19.057831100003416	15.718744388229863
11	30.91330306749559	25.2027657771439	23.53596801457984	20.34796314078067
12	27.81879677711867	21.596837076492232	30.399802910922563	20.184563235466534
13	24.839160174946237	24.879379406862796	29.482181099335563	20.799279318855397
14	18.485708815729133	29.049119406595658	28.76521021598173	23.699961561693485
15	18.216047028303358	25.684060866093706	35.59327819390419	20.50661391169875
16	19.545211017398156	25.43354402670201	32.3128136098319	22.70843134606794
17	19.99385580737511	25.813920009735725	27.55685233308648	26.635371849802684
18	20.639441264338306	25.360377674912847	31.16055487699	22.839626183758853
19	22.891718251665534	26.723972888379016	27.666676065995162	22.717632793960288
20	24.458635781462647	26.62038239436515	27.64946045380947	21.27152137036273
21	22.735738868845235	27.52093700421634	28.17260728962448	21.57071683731395
22	20.283998236883853	26.90993118207439	25.85770109244932	26.948369488592434
23	20.948283410531502	27.180928663549057	27.27902797091749	24.59175995500195
24	22.600091717658024	24.61891906732937	28.245773641413642	24.53521557359897
25	21.02545684446733	26.70556999259432	26.229914500739827	26.03905866219852
26	21.105153256051064	28.79311138055853	26.44510965306089	23.656625710329514
27	22.065665687652398	28.190119722709916	27.67350294668948	22.070711642948204
28	18.929604471310032	27.142193536131266	29.53353111499287	24.39467087756583
29	21.291111549746443	26.525696527343882	28.28718015692921	23.896011765980465
30	22.2400479662574	28.164444714881263	26.217151202050438	23.3783561168109
31	22.672961248547434	28.082967377899017	24.704848717807916	24.539222655745636
32	24.85607896623217	28.56099743695153	23.660929613375934	22.921993983440363
33	22.81335753413069	27.482201876798552	25.393621615685202	24.310818973385555
34	21.46950091049811	24.461752400910054	27.88944015125993	26.179306537331907
35	23.93504371429801	25.03046124483717	25.363197473460502	25.671297567404316
36	22.880884288824543	27.467657652710642	26.662530962130106	22.988927096334706
37	21.460596283505513	27.486357369395094	28.428615315661602	22.624431031437787
38	21.471430246346507	28.343724538332193	26.82103332259831	23.363811892722993
39	22.909230684750977	24.16359580710797	27.969433383743414	24.95774012439764
40	24.65631850069827	22.876580385778123	28.112946288774086	24.35415482474952
41	21.88208196115505	24.240472420144048	24.923160489576393	28.954285129124514
42	23.848075190670325	24.129461403636355	26.10109423024694	25.92136917544638
43	23.196108084362436	24.735421270482497	27.519749720617327	24.54872092453774
44	21.10500484560119	24.412480131551025	28.03473398168912	26.44778104115867
45	22.46815482771773	23.281147272141727	27.237621455401918	27.013076444738626
46	23.3555009075299	24.305476197189996	28.15286869979089	24.186154195489213
47	21.171047495796273	23.921686773809117	30.01185799494514	24.895407735449467
48	21.273599116661003	22.615377993995313	29.149148049812485	26.961874839531202
49	22.167920487617373	21.899297573340736	30.790419214997765	25.142362724044126
50	20.699695906988204	22.12280371085489	29.78286067078555	27.394639711371354
51	20.682777115702272	21.669261376032008	26.37491151026926	31.27304999799646
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	412.0
1	614.0
2	816.0
3	1051.0
4	1286.0
5	1038.5
6	791.0
7	791.0
8	791.0
9	887.0
10	983.0
11	991.5
12	1000.0
13	1190.5
14	1381.0
15	1433.5
16	1486.0
17	1526.5
18	1567.0
19	1766.0
20	1965.0
21	1857.0
22	1749.0
23	1942.5
24	2136.0
25	3394.0
26	4753.0
27	4854.0
28	6347.0
29	7840.0
30	9091.0
31	10342.0
32	11361.5
33	12381.0
34	13227.0
35	14073.0
36	15586.5
37	17100.0
38	18815.5
39	20531.0
40	22666.0
41	24801.0
42	29624.5
43	34448.0
44	40174.5
45	45901.0
46	54246.0
47	62591.0
48	71620.0
49	80649.0
50	78599.5
51	76550.0
52	64133.5
53	51717.0
54	44629.5
55	37542.0
56	33652.0
57	29762.0
58	27739.0
59	25716.0
60	25372.5
61	25029.0
62	23044.0
63	21059.0
64	18417.0
65	15775.0
66	12986.5
67	10198.0
68	9029.5
69	7861.0
70	6655.5
71	5450.0
72	4637.5
73	3825.0
74	3336.0
75	2261.5
76	1676.0
77	1376.0
78	1076.0
79	871.0
80	666.0
81	519.0
82	372.0
83	234.0
84	96.0
85	67.0
86	38.0
87	26.5
88	15.0
89	9.5
90	4.0
91	2.5
92	1.0
93	1.0
94	1.0
95	2.0
96	3.0
97	2.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	673807.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.701709960234478
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.9342335867867	18.818944351919846
2	13.027658694342929	7.217768452232296
3	5.401994998143715	4.489334959356437
4	2.841417053940889	3.148484444173377
5	1.654908917991315	2.292190342840043
6	1.031789265660734	1.7149396186450185
7	0.7453074184764548	1.4452402956492099
8	0.5256410882367831	1.1648925575613907
9	0.43038839669272966	1.0730245081889105
>10	5.643045279048803	39.62754989582989
>50	0.6739991691165239	11.62874842123696
>100	0.08049353134350438	3.95315308992879
>500	0.005902858965190321	1.0813084486948452
>1k	0.003219741253740175	2.3444206137429755
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTCTGC	4068	0.6037337100979954	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTCTGC	3799	0.5638112990811909	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTCTGCT	3683	0.5465956868955056	Illumina Single End Adapter 1 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTCTGC	1843	0.2735204591225677	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCC	1286	0.19085583854130336	No Hit
ACTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTCTGC	1092	0.1620642112652436	Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTCTG	996	0.14781680807709033	No Hit
GCCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTCTG	917	0.1360923825368392	No Hit
GGCTGTCTCTTATACACATCTGACGCGATATCGCTCGTATGCCGTCTTCTG	687	0.10195797906522193	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	679	0.10077069546620918	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.936417995063869E-4	0.0	0.0	0.569302485726625	0.0
2	5.936417995063869E-4	0.0	0.0	2.2678600845642745	0.0
3	5.936417995063869E-4	0.0	0.0	3.1063791263670457	0.0
4	5.936417995063869E-4	0.0	0.0	3.984375347836992	0.0
5	5.936417995063869E-4	0.0	0.0	6.170906505868891	0.0
6	5.936417995063869E-4	0.0	0.0	7.303723469776954	0.0
7	7.420522493829835E-4	0.0	0.0	8.598456234500384	0.0
8	7.420522493829835E-4	0.0	0.0	10.59279585994209	0.0
9	7.420522493829835E-4	0.0	0.0	11.279342601071226	0.0
10	7.420522493829835E-4	0.0	0.0	13.005059312236293	0.0
11	7.420522493829835E-4	0.0	0.0	15.64171936474391	0.0
12	7.420522493829835E-4	0.0	0.0	17.500857070348037	0.0
13	7.420522493829835E-4	0.0	0.0	18.220499341799655	0.0
14	7.420522493829835E-4	0.0	0.0	18.51420362210544	0.0
15	7.420522493829835E-4	0.0	0.0	19.054566070106127	0.0
16	7.420522493829835E-4	0.0	0.0	20.265150109749527	0.0
17	7.420522493829835E-4	0.0	0.0	21.614943151377176	0.0
18	7.420522493829835E-4	0.0	0.0	23.27714018999506	0.0
19	7.420522493829835E-4	0.0	0.0	24.111503739201286	0.0
20	8.904626992595802E-4	0.0	0.0	24.92538664632454	0.0
21	0.0019293358483957572	0.0	0.0	25.93398406368589	0.0
22	0.0026713880977787406	0.0	0.0	26.924178585262545	0.0
23	0.0032650298972851277	0.0	0.0	27.85233753879078	0.0
24	0.0051943657456808845	0.0	0.0	28.572128220692274	0.0
25	0.0072721120439532385	0.0	0.0	29.23344518534239	0.0
26	0.0072721120439532385	0.0	0.0	29.818479178755936	0.0
27	0.0072721120439532385	0.0	0.0	30.401287015421328	0.0
28	0.007420522493829835	0.0	0.0	30.9677697026003	0.0
29	0.007420522493829835	0.0	0.0	31.553990979612855	0.0
30	0.007420522493829835	0.0	0.0	32.20670013817013	0.0
31	0.007420522493829835	0.0	0.0	32.798264191378244	0.0
32	0.007420522493829835	0.0	0.0	33.368308729354254	0.0
33	0.007420522493829835	0.0	0.0	33.91816944614704	0.0
34	0.008904626992595803	0.0	0.0	34.48554259602527	0.0
35	0.009349858342225592	0.0	0.0	35.09194769422105	0.0
36	0.009349858342225592	0.0	0.0	35.67965307573237	0.0
37	0.00994350014173198	0.0	0.0	36.21244659078935	0.0
38	0.00994350014173198	0.0	0.0	36.739600508751025	0.0
39	0.010091910591608577	0.0	0.0	37.27001945660998	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAAAT	20	7.031206E-4	45.0	33
ACACGTG	45	3.8380676E-10	45.0	42
TAGCGGA	20	7.031206E-4	45.0	26
CACTCGC	25	3.8890816E-5	45.0	22
TAATTCG	20	7.031206E-4	45.0	12
TGCGATG	40	6.8084773E-9	45.0	1
TCGCTCA	20	7.031206E-4	45.0	28
TCGTCCC	75	0.0	45.0	38
AATTCCG	20	7.031206E-4	45.0	18
TTACGCA	20	7.031206E-4	45.0	1
CGCCGTT	20	7.031206E-4	45.0	26
CCTATCG	20	7.031206E-4	45.0	12
ATGCACG	20	7.031206E-4	45.0	1
GACCTAG	50	2.1827873E-11	45.0	1
ACGTACG	30	2.164159E-6	44.999996	21
AAATGCG	30	2.164159E-6	44.999996	1
TACGAAT	120	0.0	43.124996	12
TAGCGAG	85	0.0	42.35294	1
AAGTACG	70	0.0	41.785713	1
AAGCTAC	125	0.0	41.399998	8
>>END_MODULE