FastQCFastQC Report
Sat 18 Jun 2016
SRR3552815_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552815_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences912857
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGC51220.5610955494672222No Hit
GCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGC48110.5270266865456474No Hit
CTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGCT41110.4503443584263472TruSeq Adapter, Index 21 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGC22680.24845074310653256No Hit
ACTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGC14800.16212835088080607TruSeq Adapter, Index 21 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCC10940.11984352423216342No Hit
GCCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTG9960.1091079982954614No Hit
CGCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTG9830.10768389791610297TruSeq Adapter, Index 21 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCCGT302.1650085E-645.0000049
GTCGTAG302.1650085E-645.0000041
ACTCGAT302.1650085E-645.00000416
TGTACCG302.1650085E-645.0000041
AATCACG207.032523E-445.011
ACGTTAG207.032523E-445.01
GACACGC207.032523E-445.011
CACGATC207.032523E-445.016
ATATGCG253.8901722E-545.01
ACGATTG207.032523E-445.01
TCAGCGA207.032523E-445.039
CTCGATC502.1827873E-1145.016
ATAACGG207.032523E-445.02
GACGAAT207.032523E-445.09
CGATCTC253.8901722E-545.010
TAAGACG406.8121153E-945.01
CCGTTCA207.032523E-445.014
CGTTAGG1200.043.1250042
AGTACGG750.042.02
GCCGATA700.041.7857179