##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3552815_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 912857 Sequences flagged as poor quality 0 Sequence length 51 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.656157536174888 31.0 31.0 34.0 30.0 34.0 2 31.83718479455161 31.0 31.0 34.0 30.0 34.0 3 31.878010466042326 33.0 31.0 34.0 30.0 34.0 4 35.48802057715502 37.0 35.0 37.0 33.0 37.0 5 35.37828926107813 37.0 35.0 37.0 33.0 37.0 6 35.35149536017142 37.0 35.0 37.0 33.0 37.0 7 35.75709338921649 37.0 35.0 37.0 35.0 37.0 8 35.74325989722377 37.0 35.0 37.0 35.0 37.0 9 37.348624154714265 39.0 37.0 39.0 35.0 39.0 10 36.77998196869827 39.0 37.0 39.0 32.0 39.0 11 36.572268164674206 38.0 35.0 39.0 32.0 39.0 12 36.341368911012346 38.0 35.0 39.0 32.0 39.0 13 36.24255277661233 38.0 35.0 39.0 32.0 39.0 14 37.23478157038835 39.0 36.0 40.0 32.0 41.0 15 37.3155072481232 39.0 36.0 41.0 32.0 41.0 16 37.397569389290986 39.0 36.0 41.0 32.0 41.0 17 37.35345842777127 39.0 36.0 41.0 32.0 41.0 18 37.3539009943507 39.0 36.0 41.0 32.0 41.0 19 37.349927754292295 39.0 35.0 41.0 32.0 41.0 20 37.283703800266636 39.0 35.0 40.0 32.0 41.0 21 37.216350425093964 39.0 35.0 40.0 32.0 41.0 22 37.147116141958705 39.0 35.0 40.0 32.0 41.0 23 37.120093289529464 39.0 35.0 40.0 32.0 41.0 24 37.1049014248672 39.0 35.0 40.0 32.0 41.0 25 37.05958545533419 39.0 35.0 40.0 32.0 41.0 26 36.95550124499237 39.0 35.0 40.0 31.0 41.0 27 36.8634747830164 39.0 35.0 40.0 31.0 41.0 28 36.805947700461296 39.0 35.0 40.0 31.0 41.0 29 36.785153643999 39.0 35.0 40.0 31.0 41.0 30 36.646617159095015 38.0 35.0 40.0 31.0 41.0 31 36.521333571413706 38.0 35.0 40.0 30.0 41.0 32 36.301460141073576 38.0 35.0 40.0 30.0 41.0 33 36.23151380774864 38.0 35.0 40.0 30.0 41.0 34 36.09390298809124 38.0 35.0 40.0 30.0 41.0 35 35.96515883648808 38.0 35.0 40.0 30.0 41.0 36 35.89678668181325 38.0 35.0 40.0 30.0 41.0 37 35.84583675208713 38.0 34.0 40.0 29.0 41.0 38 35.752632668643614 38.0 34.0 40.0 29.0 41.0 39 35.80886381985349 38.0 34.0 40.0 29.0 41.0 40 35.65496786462721 38.0 34.0 40.0 29.0 41.0 41 35.443467049055876 38.0 34.0 40.0 27.0 41.0 42 35.36612634837658 38.0 34.0 40.0 27.0 41.0 43 35.366864689650185 38.0 34.0 40.0 27.0 41.0 44 35.281799887605615 38.0 34.0 40.0 27.0 41.0 45 35.24265136817705 37.0 34.0 40.0 27.0 41.0 46 35.06475493971126 37.0 34.0 40.0 26.0 41.0 47 34.91327119143524 37.0 33.0 40.0 26.0 41.0 48 34.82534613855182 37.0 33.0 40.0 26.0 41.0 49 34.82648980070263 37.0 33.0 40.0 26.0 41.0 50 34.71827460379884 37.0 33.0 40.0 26.0 41.0 51 33.10962396081752 35.0 31.0 39.0 22.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 2.0 10 4.0 11 6.0 12 6.0 13 5.0 14 23.0 15 46.0 16 96.0 17 207.0 18 364.0 19 666.0 20 1168.0 21 1657.0 22 2481.0 23 3475.0 24 4912.0 25 6756.0 26 8423.0 27 10611.0 28 13568.0 29 17454.0 30 22237.0 31 27925.0 32 36132.0 33 48688.0 34 76314.0 35 84963.0 36 86066.0 37 117609.0 38 171996.0 39 168968.0 40 28.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.77708009030987 20.83404081909872 25.669847522667844 11.719031567923563 2 30.3047465265644 25.099002362911165 26.02422942476204 18.57202168576239 3 32.15914431285513 23.857077285927588 26.648094937104062 17.33568346411322 4 28.241444169240086 26.991850859444575 26.1174532265185 18.64925174479683 5 25.127374824315307 29.839065702514194 24.732241742134857 20.30131773103564 6 26.79466772999495 35.14263460761105 23.4562478022297 14.606449860164297 7 80.78833815153962 5.851080727868658 8.118686716539392 5.241894404052332 8 81.14195323035261 4.713991348042464 7.901894820327827 6.24216060127709 9 75.73847820633462 6.937559771136115 10.595635460975815 6.728326561553452 10 43.52927128783588 23.735261930400927 17.142224904886525 15.593241876876663 11 32.286327431350145 25.093196415210706 22.30239785640029 20.31807829703886 12 28.63427678157696 21.839784325474856 29.529597735461305 19.99634115748688 13 24.553791009982945 24.656983514394916 29.98761032669958 20.801615148922558 14 19.332819926888877 28.288987212674055 28.79092782330639 23.587265037130678 15 19.211771394643414 25.614636246421945 34.30405857653499 20.869533782399653 16 21.391083159793922 24.276967805472268 31.408095682018107 22.923853352715703 17 20.543414795526573 25.218955433326357 28.13759438772995 26.10003538341712 18 22.082976851796065 25.066576692735005 29.81956648193529 23.03087997353364 19 23.297405836839722 26.518830441131524 26.446968145065437 23.736795576963317 20 25.432570490230123 26.142977487163925 27.16186653550337 21.26258548710258 21 23.887092940077142 27.414808672114034 27.145872792781343 21.55222559502748 22 21.620691959419712 26.346842933778237 25.173712859735968 26.858752247066082 23 21.741521399299124 27.067218633367546 26.42429208517873 24.766967882154596 24 23.758923905934886 24.08800063974971 27.802163975299525 24.35091147901588 25 22.127452602105258 25.281834942384187 26.241240413339657 26.349472042170895 26 21.49175610199626 27.19900269154972 27.368580182876396 23.940661023577626 27 22.23338376109292 26.583024504385683 28.686968495613225 22.496623238908175 28 19.52792167886098 26.447406329797545 30.034057908303275 23.990614083038196 29 24.51260164516458 23.932335513667528 27.58142841649897 23.973634424668923 30 25.99213239313496 24.162711136574515 26.81646742041744 23.02868904987309 31 26.277719292287838 25.766029071366052 25.176670606677714 22.779581029668392 32 27.500583333424622 24.400316807561314 26.504041706422804 21.59505815259126 33 27.043118473101487 23.578391796305446 26.74142828504355 22.637061445549524 34 24.541521837483856 23.376607727168658 29.479316037451646 22.602554397895837 35 25.553947661024672 22.265590338903028 28.624198532738426 23.556263467333878 36 25.40156892043332 24.8214123351193 29.262962325972197 20.51405641847518 37 24.98606024820974 25.023086858073064 30.75640543918708 19.23444745453012 38 24.789643942041305 25.69624815277749 27.99945665093218 21.514651254249024 39 27.43748473200074 22.464745299647152 28.236952775735958 21.86081719261615 40 27.94424537468629 21.643148926940363 28.4985490608058 21.91405663756755 41 22.130629441413056 23.149299397386446 29.307109437732304 25.412961723468186 42 22.70081732407157 22.339314920080582 30.943181681249087 24.01668607459876 43 24.475575035301258 21.20989377306632 30.57510650627645 23.739424685355974 44 23.763086660889932 22.35388456242325 28.471600699781018 25.4114280769058 45 25.930129253541356 21.18327405059062 26.85831406233397 26.02828263353406 46 25.324777046130993 23.080285302079076 28.75412030580912 22.840817345980806 47 22.07738999646166 22.57297692847839 32.34372963125659 23.005903443803355 48 22.195809420314465 20.777514988656492 32.079613783977116 24.947061807051927 49 24.00277370935426 20.26034745858333 32.57980165568101 23.157077176381407 50 22.909721895105147 20.14203758091355 30.596139373417742 26.352101150563563 51 22.037515185839624 19.882194034772148 28.447390993331922 29.632899786056306 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 412.0 1 567.5 2 723.0 3 1084.5 4 1446.0 5 1120.0 6 794.0 7 862.5 8 931.0 9 1046.5 10 1162.0 11 1237.5 12 1313.0 13 1448.5 14 1584.0 15 1640.5 16 1697.0 17 1829.5 18 1962.0 19 1822.5 20 1683.0 21 1777.5 22 1872.0 23 2327.5 24 2783.0 25 3449.5 26 5395.0 27 6674.0 28 7063.5 29 7453.0 30 9842.5 31 12232.0 32 13066.0 33 13900.0 34 17187.0 35 20474.0 36 20768.5 37 21063.0 38 23242.0 39 25421.0 40 29887.5 41 34354.0 42 39096.0 43 43838.0 44 51222.5 45 58607.0 46 69596.0 47 80585.0 48 90913.5 49 101242.0 50 102049.5 51 102857.0 52 88512.0 53 74167.0 54 66489.5 55 58812.0 56 53017.5 57 47223.0 58 45627.5 59 44032.0 60 41225.5 61 38419.0 62 34622.0 63 30825.0 64 26008.0 65 21191.0 66 17141.0 67 13091.0 68 12054.0 69 11017.0 70 9570.5 71 8124.0 72 6635.5 73 5147.0 74 4548.5 75 3190.5 76 2431.0 77 1960.0 78 1489.0 79 1174.5 80 860.0 81 611.5 82 363.0 83 336.5 84 310.0 85 220.5 86 131.0 87 81.5 88 32.0 89 23.5 90 15.0 91 10.0 92 5.0 93 4.5 94 4.0 95 22.0 96 40.0 97 20.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 912857.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.938585070411378 #Duplication Level Percentage of deduplicated Percentage of total 1 66.54059933202522 16.594283970778694 2 13.325825750435408 6.64654478221424 3 5.898274935431327 4.41283893737788 4 3.121725126812101 3.114056305657773 5 1.8086379357753744 2.25524355114537 6 1.147910697298409 1.717636114668697 7 0.7945479093559459 1.3870430440993538 8 0.5886897291696386 1.1744871112779574 9 0.4412837375197317 0.9904492825492421 >10 4.801882491490536 31.166881619475873 >50 1.4259418604466036 23.202711817189282 >100 0.09588381405145789 4.202161784372994 >500 0.006157676131745002 1.064086960851016 >1k 0.0021991700470517864 1.5097508431247688 >5k 4.3983400941035726E-4 0.5618238752168748 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGC 5122 0.5610955494672222 No Hit GCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGC 4811 0.5270266865456474 No Hit CTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGCT 4111 0.4503443584263472 TruSeq Adapter, Index 21 (95% over 23bp) TCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGC 2268 0.24845074310653256 No Hit ACTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGC 1480 0.16212835088080607 TruSeq Adapter, Index 21 (95% over 22bp) CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCC 1094 0.11984352423216342 No Hit GCCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTG 996 0.1091079982954614 No Hit CGCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTG 983 0.10768389791610297 TruSeq Adapter, Index 21 (95% over 21bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.0954618302757168E-4 0.0 0.0 0.47553998052268864 0.0 2 1.0954618302757168E-4 0.0 0.0 2.0858688710279925 0.0 3 1.0954618302757168E-4 0.0 0.0 2.795399498497574 0.0 4 1.0954618302757168E-4 0.0 0.0 3.5861038475905866 0.0 5 1.0954618302757168E-4 0.0 0.0 5.591456274093313 0.0 6 1.0954618302757168E-4 0.0 0.0 6.647810117028187 0.0 7 1.0954618302757168E-4 0.0 0.0 7.796949576987414 0.0 8 1.0954618302757168E-4 0.0 0.0 9.599970203438216 0.0 9 1.0954618302757168E-4 0.0 0.0 10.215510205870142 0.0 10 1.0954618302757168E-4 0.0 0.0 11.789469763610292 0.0 11 1.0954618302757168E-4 0.0 0.0 14.093664177412235 0.0 12 1.0954618302757168E-4 0.0 0.0 15.787248167018493 0.0 13 1.0954618302757168E-4 0.0 0.0 16.4297365304752 0.0 14 1.0954618302757168E-4 0.0 0.0 16.685198229295498 0.0 15 1.0954618302757168E-4 0.0 0.0 17.18034697658012 0.0 16 1.0954618302757168E-4 0.0 0.0 18.25094182330858 0.0 17 1.0954618302757168E-4 0.0 0.0 19.504259703327026 0.0 18 1.0954618302757168E-4 0.0 0.0 20.955965720808408 0.0 19 1.0954618302757168E-4 0.0 0.0 21.79541812134869 0.0 20 1.0954618302757168E-4 0.0 0.0 22.55544953919398 0.0 21 0.0025195622096341485 0.0 0.0 23.470926990755398 0.0 22 0.0029577469417444353 0.0 0.0 24.44972213610675 0.0 23 0.003724570222937437 0.0 0.0 25.373306005212207 0.0 24 0.006134586249544014 0.0 0.0 26.1226018971208 0.0 25 0.011502349217895027 0.0 0.0 26.772210762474298 0.0 26 0.011502349217895027 0.0 0.0 27.35324371725254 0.0 27 0.01172144158395017 0.0 0.0 27.913572443438568 0.0 28 0.01172144158395017 0.0 0.0 28.472148430696155 0.0 29 0.01172144158395017 0.0 0.0 29.0682987587322 0.0 30 0.01172144158395017 0.0 0.0 29.709582114175603 0.0 31 0.01172144158395017 0.0 0.0 30.354809132208 0.0 32 0.01172144158395017 0.0 0.0 30.983823315152318 0.0 33 0.01172144158395017 0.0 0.0 31.541522932945686 0.0 34 0.011940533950005314 0.0 0.0 32.139206907544114 0.0 35 0.011940533950005314 0.0 0.0 32.75748556455173 0.0 36 0.011940533950005314 0.0 0.0 33.35659363952952 0.0 37 0.012050080133032885 0.0 0.0 33.93565476301327 0.0 38 0.013364634329363745 0.0 0.0 34.528737797924535 0.0 39 0.013693272878446459 0.0 0.0 35.127626780536275 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTCCGT 30 2.1650085E-6 45.000004 9 GTCGTAG 30 2.1650085E-6 45.000004 1 ACTCGAT 30 2.1650085E-6 45.000004 16 TGTACCG 30 2.1650085E-6 45.000004 1 AATCACG 20 7.032523E-4 45.0 11 ACGTTAG 20 7.032523E-4 45.0 1 GACACGC 20 7.032523E-4 45.0 11 CACGATC 20 7.032523E-4 45.0 16 ATATGCG 25 3.8901722E-5 45.0 1 ACGATTG 20 7.032523E-4 45.0 1 TCAGCGA 20 7.032523E-4 45.0 39 CTCGATC 50 2.1827873E-11 45.0 16 ATAACGG 20 7.032523E-4 45.0 2 GACGAAT 20 7.032523E-4 45.0 9 CGATCTC 25 3.8901722E-5 45.0 10 TAAGACG 40 6.8121153E-9 45.0 1 CCGTTCA 20 7.032523E-4 45.0 14 CGTTAGG 120 0.0 43.125004 2 AGTACGG 75 0.0 42.0 2 GCCGATA 70 0.0 41.785717 9 >>END_MODULE