##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552814_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	363668
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.027087893353276	33.0	33.0	33.0	27.0	33.0
2	32.1727179735363	33.0	33.0	33.0	33.0	33.0
3	32.185691344853	33.0	33.0	33.0	33.0	33.0
4	32.11964209113807	33.0	33.0	33.0	33.0	33.0
5	32.14231386869342	33.0	33.0	33.0	33.0	33.0
6	35.65428357732877	37.0	37.0	37.0	33.0	37.0
7	35.81604925371493	37.0	37.0	37.0	33.0	37.0
8	35.85499136575118	37.0	37.0	37.0	37.0	37.0
9	35.75461134881265	37.0	37.0	37.0	37.0	37.0
10	35.83807483748914	37.0	37.0	37.0	33.0	37.0
11	35.83074122551338	37.0	37.0	37.0	33.0	37.0
12	35.81584027189634	37.0	37.0	37.0	33.0	37.0
13	35.83991167768404	37.0	37.0	37.0	33.0	37.0
14	35.892792327067546	37.0	37.0	37.0	37.0	37.0
15	35.929933345798915	37.0	37.0	37.0	37.0	37.0
16	35.97172970951527	37.0	37.0	37.0	37.0	37.0
17	35.96015871619169	37.0	37.0	37.0	37.0	37.0
18	35.94080040036517	37.0	37.0	37.0	37.0	37.0
19	35.924728048659766	37.0	37.0	37.0	37.0	37.0
20	35.90828997877185	37.0	37.0	37.0	37.0	37.0
21	35.81024450872774	37.0	37.0	37.0	37.0	37.0
22	35.8497228241143	37.0	37.0	37.0	37.0	37.0
23	35.879186510773565	37.0	37.0	37.0	37.0	37.0
24	35.876126027035646	37.0	37.0	37.0	37.0	37.0
25	35.87187489688397	37.0	37.0	37.0	37.0	37.0
26	35.82193374176447	37.0	37.0	37.0	33.0	37.0
27	35.81284303265616	37.0	37.0	37.0	33.0	37.0
28	35.79725463884642	37.0	37.0	37.0	33.0	37.0
29	35.7869595345205	37.0	37.0	37.0	33.0	37.0
30	35.7551530516845	37.0	37.0	37.0	33.0	37.0
31	35.74322733922149	37.0	37.0	37.0	33.0	37.0
32	35.71611469802127	37.0	37.0	37.0	33.0	37.0
33	35.68731370370778	37.0	37.0	37.0	33.0	37.0
34	35.640152556727564	37.0	37.0	37.0	33.0	37.0
35	35.6311993356578	37.0	37.0	37.0	33.0	37.0
36	35.6057530494847	37.0	37.0	37.0	33.0	37.0
37	35.60850830977705	37.0	37.0	37.0	33.0	37.0
38	35.5790528724001	37.0	37.0	37.0	33.0	37.0
39	35.6021536126357	37.0	37.0	37.0	33.0	37.0
40	35.57854691641827	37.0	37.0	37.0	33.0	37.0
41	35.565678036010866	37.0	37.0	37.0	33.0	37.0
42	35.54559103358008	37.0	37.0	37.0	33.0	37.0
43	35.56264779964143	37.0	37.0	37.0	33.0	37.0
44	35.53599986801148	37.0	37.0	37.0	33.0	37.0
45	35.49744547224392	37.0	37.0	37.0	33.0	37.0
46	35.51073231628848	37.0	37.0	37.0	33.0	37.0
47	35.50422913206551	37.0	37.0	37.0	33.0	37.0
48	35.49720074353531	37.0	37.0	37.0	33.0	37.0
49	35.4879505483023	37.0	37.0	37.0	33.0	37.0
50	35.47532639660349	37.0	37.0	37.0	33.0	37.0
51	35.16986922137774	37.0	37.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	309.0
15	1145.0
16	896.0
17	556.0
18	437.0
19	434.0
20	456.0
21	507.0
22	596.0
23	746.0
24	933.0
25	1291.0
26	1706.0
27	2539.0
28	3382.0
29	4011.0
30	4951.0
31	5764.0
32	7732.0
33	10914.0
34	17048.0
35	37954.0
36	259361.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.91790314242661	19.396812477314473	23.61219573897071	15.073088641288207
2	28.68687924150599	24.913657511796476	26.016586556969546	20.382876689727993
3	32.22967101862138	23.753808418667578	26.134001341883256	17.88251922082779
4	29.214833309502076	26.327309524071406	25.698714211863567	18.75914295456295
5	25.356094019820276	29.575326946555652	24.960403444900294	20.108175588723782
6	26.62950823278375	35.2973041345403	23.190382436728004	14.88280519594795
7	80.23774431624449	6.3195002034822965	8.239383173663892	5.203372306609325
8	81.1341663275295	4.95176919607994	7.787872455096406	6.126192021294147
9	75.95911655685956	7.0044656114918	10.551107053686328	6.485310777962318
10	43.67362539459067	23.722186169803226	17.035592903417403	15.5685955321887
11	32.619862071999734	25.177084593640352	22.287360999593037	19.915692334766874
12	28.773221729709515	22.05033162114896	29.348196706886505	19.828249942255024
13	24.994775454535457	24.62768239163193	29.63417182705105	20.74337032678157
14	19.766380324911733	28.022537039277584	28.787245509640663	23.423837126170024
15	19.405886687858157	25.161410957246723	34.40170705148652	21.030995303408602
16	21.79130415653838	23.7505087057426	31.745713122958303	22.712474014760716
17	20.999373054544254	24.77699440148707	28.40915340365388	25.814479140314795
18	22.474069755931232	24.38350363518374	30.14727718688474	22.995149422000285
19	23.872323107889613	25.846926317410386	26.76259665409109	23.518153920608906
20	25.829602824554264	25.37369248875348	27.591374550414116	21.205330136278143
21	24.16297282136454	27.000450960766415	27.17588569794428	21.660690519924767
22	21.874896883971097	26.154899523741438	25.650868374451424	26.31933521783605
23	21.893595257212624	26.76782119955564	26.617684261469254	24.720899281762488
24	23.55280090632115	23.92924315584544	28.10667971886446	24.41127621896895
25	21.9351166448519	24.841063827447012	26.95755469274173	26.266264834959358
26	21.569123486256693	27.288625889547607	27.55397780393106	23.588272820264635
27	22.155372482594014	26.236292442557495	29.193385175489734	22.414949899358756
28	19.61679333897951	26.113653112179243	30.683480537193265	23.586073011647986
29	24.074980476698528	23.97516416071802	28.07918211115633	23.870673251427128
30	25.666816986922136	24.03015937613428	27.256178712452016	23.04684492449157
31	26.292937514436243	25.803755073308622	25.212281531506758	22.691025880748374
32	26.938856319500204	24.36260545332556	26.970478568364552	21.728059658809684
33	26.59238646237777	23.80577889723594	26.99550139137895	22.60633324900734
34	24.02713463928638	23.55720052355445	29.685042401311083	22.73062243584808
35	25.38854119691587	22.49689277032898	28.527393116798837	23.587172915956312
36	25.35086947435573	24.98955090907091	29.076795318807264	20.582784297766093
37	25.073968564734866	25.25710263207101	30.440951637207565	19.22797716598656
38	24.46902119515602	26.119977561952112	28.046459957983654	21.364541284908213
39	26.99962603253517	22.715773727685693	28.335184838919012	21.949415400860126
40	27.875974790193254	21.75500731436365	28.363232398781307	22.00578549666179
41	21.97196343918079	23.35536808297678	29.257454601449673	25.415213876392755
42	22.799916407272566	22.64592980410704	30.55726651781295	23.99688727080744
43	24.376354257179624	21.200930519044846	30.802270202492384	23.62044502128315
44	23.681764686472278	22.46857023438961	28.8469153183673	25.002749760770815
45	25.560951197245842	21.739058701892937	27.017499477545453	25.682490623315772
46	24.890559521321645	23.41641277208883	28.945356753962404	22.74767095262712
47	22.336581717390587	23.043270235489512	31.799883410143316	22.820264636976585
48	22.282411430205574	21.313120758494012	31.624723649042537	24.779744162257884
49	24.067831098694413	20.88965759978882	32.05781097044557	22.9847003310712
50	23.018522388552196	20.824763245597634	30.381831780635082	25.774882585215085
51	22.09047812840283	20.608082096857576	28.450399815216077	28.851039959523522
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	150.0
1	205.5
2	261.0
3	647.5
4	1034.0
5	790.5
6	547.0
7	537.0
8	527.0
9	578.0
10	629.0
11	666.5
12	704.0
13	760.5
14	817.0
15	817.0
16	817.0
17	841.5
18	866.0
19	783.0
20	700.0
21	749.5
22	799.0
23	948.5
24	1098.0
25	1434.5
26	2278.0
27	2785.0
28	2877.0
29	2969.0
30	3683.0
31	4397.0
32	4853.0
33	5309.0
34	6554.5
35	7800.0
36	8028.0
37	8256.0
38	9219.5
39	10183.0
40	12118.5
41	14054.0
42	15762.0
43	17470.0
44	19946.0
45	22422.0
46	26169.5
47	29917.0
48	34104.5
49	38292.0
50	39426.0
51	40560.0
52	35122.5
53	29685.0
54	26981.5
55	24278.0
56	21942.0
57	19606.0
58	18729.0
59	17852.0
60	16893.5
61	15935.0
62	14465.5
63	12996.0
64	10942.0
65	8888.0
66	7341.0
67	5794.0
68	5095.0
69	4396.0
70	3890.0
71	3384.0
72	2719.5
73	2055.0
74	1738.5
75	1194.5
76	967.0
77	842.0
78	717.0
79	534.0
80	351.0
81	208.5
82	66.0
83	79.5
84	93.0
85	70.5
86	48.0
87	24.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	363668.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.927216413556813
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.15830510445711	22.399593902783497
2	11.570032903445641	7.387978888405641
3	4.2970025451807	4.115739905687659
4	2.0932214706313257	2.6732293957739994
5	1.2699113899306618	2.0272367886178486
6	0.8897880554064849	1.704507348429643
7	0.7054285136536088	1.576565817379874
8	0.629626804574992	1.6081784999553608
9	0.5340192282634209	1.5344772722790148
>10	7.725188470186926	48.78151098764234
>50	0.08268682006647092	1.803422674187798
>100	0.04048208899087639	2.3525893531071382
>500	0.001722642084718144	0.32147012902188715
>1k	0.0025839631270772163	1.7134990367282967
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGC	3027	0.8323525853250767	No Hit
CTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGCT	1619	0.44518626879461487	TruSeq Adapter, Index 21 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGC	1585	0.4358370821738509	No Hit
TCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGC	664	0.18258411518197917	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	505	0.13886291892605343	No Hit
ACTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGC	462	0.1270389476115578	TruSeq Adapter, Index 21 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCC	426	0.1171398088366312	No Hit
CGCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTG	408	0.11219023944916792	TruSeq Adapter, Index 21 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTG	404	0.11109033514084274	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.45316057502997237	0.0
2	0.0	0.0	0.0	2.0584709130305665	0.0
3	0.0	0.0	0.0	2.6939406271654365	0.0
4	0.0	0.0	0.0	3.348383690618916	0.0
5	0.0	0.0	0.0	4.925646468757218	0.0
6	0.0	0.0	0.0	5.780822068480043	0.0
7	0.0	0.0	0.0	6.760836807197774	0.0
8	0.0	0.0	0.0	8.408218484991806	0.0
9	0.0	0.0	0.0	9.004916572258214	0.0
10	0.0	0.0	0.0	10.498586622963803	0.0
11	0.0	0.0	0.0	12.667047966826885	0.0
12	0.0	0.0	0.0	14.337527635095746	0.0
13	0.0	0.0	0.0	14.969697636305641	0.0
14	0.0	0.0	0.0	15.199302660668522	0.0
15	0.0	0.0	0.0	15.686010317102411	0.0
16	0.0	0.0	0.0	16.65035141942651	0.0
17	0.0	0.0	0.0	17.80195123024297	0.0
18	0.0	0.0	0.0	19.17930640034317	0.0
19	0.0	0.0	0.0	19.969312669797727	0.0
20	0.0	0.0	0.0	20.712298030071384	0.0
21	0.0016498564624877635	0.0	0.0	21.615594443283435	0.0
22	0.0021998086166503516	0.0	0.0	22.54748836851194	0.0
23	0.003024736847894233	0.0	0.0	23.402663968234762	0.0
24	0.005224545464544585	0.0	0.0	24.145924304585503	0.0
25	0.006874401927032348	0.0	0.0	24.815491052278453	0.0
26	0.006874401927032348	0.0	0.0	25.39734043138247	0.0
27	0.007149378004113642	0.0	0.0	25.959391532936635	0.0
28	0.007149378004113642	0.0	0.0	26.48294598369942	0.0
29	0.007149378004113642	0.0	0.0	27.024923831626648	0.0
30	0.007149378004113642	0.0	0.0	27.653794119911566	0.0
31	0.007149378004113642	0.0	0.0	28.30191273359218	0.0
32	0.007149378004113642	0.0	0.0	28.924183596027145	0.0
33	0.007149378004113642	0.0	0.0	29.45818713771902	0.0
34	0.007149378004113642	0.0	0.0	30.035911875666816	0.0
35	0.007149378004113642	0.0	0.0	30.59988780976055	0.0
36	0.007149378004113642	0.0	0.0	31.151214844308544	0.0
37	0.007149378004113642	0.0	0.0	31.684943409923335	0.0
38	0.008249282312438818	0.0	0.0	32.223071592771426	0.0
39	0.008524258389520112	0.0	0.0	32.77687341201315	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAAAT	20	7.026921E-4	45.0	35
CTCACTA	25	3.8855283E-5	45.0	45
ATTAGCG	25	3.8855283E-5	45.0	1
TACCAGT	20	7.026921E-4	45.0	16
TTGTCGG	25	3.8855283E-5	45.0	1
GTCTCCC	20	7.026921E-4	45.0	30
GCCGGAC	20	7.026921E-4	45.0	19
CTGACCG	20	7.026921E-4	45.0	20
AGACGCG	20	7.026921E-4	45.0	20
CGGATGA	20	7.026921E-4	45.0	32
AATCTAC	20	7.026921E-4	45.0	44
CGTAAGG	35	1.208864E-7	45.0	2
ACGTATG	20	7.026921E-4	45.0	1
GGTTACG	25	3.8855283E-5	45.0	1
TACGTAG	25	3.8855283E-5	45.0	1
GATCGAG	30	2.1613941E-6	44.999996	1
GTTTCAA	30	2.1613941E-6	44.999996	23
GCGAATG	30	2.1613941E-6	44.999996	1
CGGGATC	30	2.1613941E-6	44.999996	6
GCGATCT	30	2.1613941E-6	44.999996	9
>>END_MODULE