SRR3552809_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3552809_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1450471
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGCT	7425	0.5119026854035689	Illumina PCR Primer Index 7 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGC	7065	0.4870831612627898	RNA PCR Primer, Index 14 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGC	6395	0.44089126911189536	Illumina PCR Primer Index 7 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGC	4000	0.2757724904531011	RNA PCR Primer, Index 14 (95% over 24bp)
ACTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGC	2439	0.1681522760537784	RNA PCR Primer, Index 14 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCC	2155	0.14857242923160824	No Hit
GCCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTG	1747	0.12044363520539192	RNA PCR Primer, Index 14 (95% over 23bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1678	0.11568655974507593	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATTGCG	50	2.1827873E-11	45.000004	1
TACGCTA	20	7.033899E-4	45.0	18
ACTACGT	20	7.033899E-4	45.0	23
CCGTTAG	20	7.033899E-4	45.0	1
TTACGAG	85	0.0	42.35294	1
GCAACGT	45	1.9288564E-8	40.000004	39
CAGTACG	85	0.0	39.705883	1
AATTGAC	40	3.459063E-7	39.375	31
AAGTACG	35	6.24942E-6	38.57143	1
TTAGGCG	35	6.24942E-6	38.57143	1
TAGCACG	155	0.0	37.741936	1
TGCGGGT	275	0.0	37.636364	4
ATTTGCG	90	0.0	37.500004	1
ATTTACG	60	1.5643309E-10	37.499996	1
TTGTCGC	30	1.14006936E-4	37.499996	42
CCTACGG	30	1.14006936E-4	37.499996	2
GGCGAAT	60	1.5643309E-10	37.499996	8
ACTTGCG	55	2.750312E-9	36.81818	1
CTAACGG	80	0.0	36.5625	2
TCAACGG	80	0.0	36.5625	2